BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.10 - phase: 0 /pseudo
(200 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y240_METJA (Q57692) Hypothetical protein MJ0240 35 0.088
YE29_METJA (Q58824) Hypothetical protein MJ1429 31 2.2
SYD_HALN1 (O07683) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 31 2.2
PNP_BACSU (P50849) Polyribonucleotide nucleotidyltransferase (EC... 31 2.2
HD12_XENLA (O42227) Probable histone deacetylase 1-2 (HD1) (RPD3... 31 2.2
FRYA_ECOLI (P77439) Putative phosphoenolpyruvate-protein phospho... 30 2.8
FRYA_ECOL6 (Q8FFD8) Putative phosphoenolpyruvate-protein phospho... 30 2.8
FRYA_ECO57 (Q8XBQ8) Putative phosphoenolpyruvate-protein phospho... 30 2.8
CBIO_METJA (Q58488) Probable cobalt transport ATP-binding protei... 30 3.7
ASP1_STRTR (P80485) Acid shock protein (T786P28D) 30 3.7
ADHX_MAIZE (P93629) Alcohol dehydrogenase class III (EC 1.1.1.1)... 30 3.7
PANE_LACLA (Q9CFY8) Putative 2-dehydropantoate 2-reductase (EC 1... 30 4.8
HD11_XENLA (Q91695) Probable histone deacetylase 1-1 (HD1) (Mate... 30 4.8
MCM4_MOUSE (P49717) DNA replication licensing factor MCM4 (CDC21... 29 6.3
HDRB_METTM (Q50755) CoB--CoM heterodisulfide reductase subunit B... 29 6.3
FRYA_SHIFL (Q83QP3) Putative phosphoenolpyruvate-protein phospho... 29 6.3
FLIG_THEMA (Q9WY63) Flagellar motor switch protein fliG 29 6.3
DCXR_MOUSE (Q91X52) L-xylulose reductase (EC 1.1.1.10) (XR) (Dic... 29 6.3
DCXR_CAVPO (Q920N9) L-xylulose reductase (EC 1.1.1.10) (XR) (Dic... 29 6.3
>Y240_METJA (Q57692) Hypothetical protein MJ0240
Length = 175
Score = 35.4 bits (80), Expect = 0.088
Identities = 32/178 (17%), Positives = 75/178 (41%), Gaps = 27/178 (15%)
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
+EVE+K+++ + + L + Q +++F+G + LR+R +D
Sbjct: 2 IEVEIKVKIDDKNKVVEQLKKLGFKFIKKKFQEDIYFNGIDRDFRETDEALRIR--DEDG 59
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVG 120
V+ K + ++ + E+++ KI ++ K+ + ++
Sbjct: 60 NFFVTYKGPKI-----DKISKTREEIEVKI-----EDKEKMRQIFKKL------------ 97
Query: 121 ENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLK 178
GF + + +R +Y + ++ +D+ G E+E SD E ++LEE ++
Sbjct: 98 --GFKEVPPIRKIREIYKKEDIEASIDDVE-GLGLFLELEKSISDINEKDKVLEEMME 152
>YE29_METJA (Q58824) Hypothetical protein MJ1429
Length = 503
Score = 30.8 bits (68), Expect = 2.2
Identities = 17/54 (31%), Positives = 29/54 (53%)
Query: 89 KIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGL 142
++G D V + LG+ E+ I+G +V + F G+ G K++ V +W GL
Sbjct: 447 ELGEDLVKDLASLGIGEAVIVGAAISLPSIVKIDKFDGVYGGKDINIVGEWMGL 500
>SYD_HALN1 (O07683) Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS)
Length = 436
Score = 30.8 bits (68), Expect = 2.2
Identities = 22/64 (34%), Positives = 29/64 (44%), Gaps = 11/64 (17%)
Query: 26 HVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDERCVVSLKAKAVLVDGVSRVEEDEED 85
HV+ H Q L D GS+LS A D + +A+ +L D V ED+ D
Sbjct: 256 HVVEHAQREL--DELGSDLSVPEA---------DFPRITFEEAREILADEYDHVPEDDND 304
Query: 86 LDPK 89
LD K
Sbjct: 305 LDTK 308
>PNP_BACSU (P50849) Polyribonucleotide nucleotidyltransferase (EC
2.7.7.8) (Polynucleotide phosphorylase) (PNPase)
(Vegetative protein 15) (VEG15)
Length = 704
Score = 30.8 bits (68), Expect = 2.2
Identities = 16/69 (23%), Positives = 37/69 (53%), Gaps = 11/69 (15%)
Query: 118 VVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFL 177
V+G +G K + + + G+K+++++ GT+F + S ++AK+I+E+ +
Sbjct: 568 VIGPSG-------KQINKIIEETGVKIDIEQD----GTIFISSTDESGNQKAKKIIEDLV 616
Query: 178 KENGIDYSY 186
+E + Y
Sbjct: 617 REVEVGQLY 625
>HD12_XENLA (O42227) Probable histone deacetylase 1-2 (HD1) (RPD3
homolog)
Length = 480
Score = 30.8 bits (68), Expect = 2.2
Identities = 31/96 (32%), Positives = 43/96 (44%), Gaps = 14/96 (14%)
Query: 80 EEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNVYDW 139
EEDEED D +I D+ RI EEF ++ G GG KNV N
Sbjct: 395 EEDEEDPDKRISIRSSDK---------RIA--CDEEFS---DSEDEGEGGRKNVANFKKV 440
Query: 140 KGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
K +K E ++ D + E E + ++KR+ EE
Sbjct: 441 KRVKTEEEKEGEDKKDVKEEEKAKDEKTDSKRVKEE 476
>FRYA_ECOLI (P77439) Putative phosphoenolpyruvate-protein
phosphotransferase fryA (EC 2.7.3.9) (Phosphotransferase
system, enzyme I)
Length = 831
Score = 30.4 bits (67), Expect = 2.8
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 4/34 (11%)
Query: 154 GTLFEIECE----SSDPEEAKRILEEFLKENGID 183
GTLF C SD E+A+R+LEE+++ ID
Sbjct: 56 GTLFNDSCSLNISGSDEEQARRVLEEYIQVRFID 89
>FRYA_ECOL6 (Q8FFD8) Putative phosphoenolpyruvate-protein
phosphotransferase fryA (EC 2.7.3.9) (Phosphotransferase
system, enzyme I)
Length = 831
Score = 30.4 bits (67), Expect = 2.8
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 4/34 (11%)
Query: 154 GTLFEIECE----SSDPEEAKRILEEFLKENGID 183
GTLF C SD E+A+R+LEE+++ ID
Sbjct: 56 GTLFNDSCSLNISGSDEEQARRVLEEYIQVRFID 89
>FRYA_ECO57 (Q8XBQ8) Putative phosphoenolpyruvate-protein
phosphotransferase fryA (EC 2.7.3.9) (Phosphotransferase
system, enzyme I)
Length = 831
Score = 30.4 bits (67), Expect = 2.8
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 4/34 (11%)
Query: 154 GTLFEIECE----SSDPEEAKRILEEFLKENGID 183
GTLF C SD E+A+R+LEE+++ ID
Sbjct: 56 GTLFNDSCSLNISGSDEEQARRVLEEYIQVRFID 89
>CBIO_METJA (Q58488) Probable cobalt transport ATP-binding protein
cbiO
Length = 279
Score = 30.0 bits (66), Expect = 3.7
Identities = 22/72 (30%), Positives = 29/72 (39%), Gaps = 11/72 (15%)
Query: 64 VSLKAKAVLVDGVSRVE-----------EDEEDLDPKIGRDCVDEPGKLGLVESRIMGRV 112
V +K K + D S VE D++ P + D P LGL + + RV
Sbjct: 61 VLIKGKPIKYDKKSLVEVRKTVGLVFQNPDDQIFAPTVKEDVAFGPLNLGLPKEEVEKRV 120
Query: 113 KEEFGVVGENGF 124
KE VG GF
Sbjct: 121 KEALKAVGMEGF 132
>ASP1_STRTR (P80485) Acid shock protein (T786P28D)
Length = 142
Score = 30.0 bits (66), Expect = 3.7
Identities = 29/103 (28%), Positives = 42/103 (40%), Gaps = 14/103 (13%)
Query: 85 DLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGLKL 144
D P + + E LVE+ + G KE V ENG + + G + + V + K KL
Sbjct: 31 DFKPNFIKTDIHETDNEYLVEAELPGIPKENIQVTYENGVLTISGQQQIDAVNEDKKGKL 90
Query: 145 EVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGIDYSYS 187
I E S ++ L E +KE+ I SYS
Sbjct: 91 --------------IRSERSLTSVQRQYLLENVKEDEIKASYS 119
>ADHX_MAIZE (P93629) Alcohol dehydrogenase class III (EC 1.1.1.1)
(Glutathione-dependent formaldehyde dehydrogenase) (EC
1.2.1.1) (FDH) (FALDH) (GSH-FDH)
Length = 381
Score = 30.0 bits (66), Expect = 3.7
Identities = 23/91 (25%), Positives = 42/91 (45%), Gaps = 8/91 (8%)
Query: 110 GRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEA 169
G V FG+ G VGL + + + + +++D FD F + E +P+E
Sbjct: 197 GSVVAVFGL----GTVGLAVAEGAKAAGASRVIGIDIDNKKFDVAKNFGVT-EFVNPKEH 251
Query: 170 KRILEEFL---KENGIDYSYSVASKFAIFRA 197
+ +++ L + G+DYS+ +I RA
Sbjct: 252 DKPIQQVLVDLTDGGVDYSFECIGNVSIMRA 282
>PANE_LACLA (Q9CFY8) Putative 2-dehydropantoate 2-reductase (EC
1.1.1.169) (Ketopantoate reductase) (KPA reductase)
(KPR)
Length = 312
Score = 29.6 bits (65), Expect = 4.8
Identities = 43/174 (24%), Positives = 76/174 (42%), Gaps = 21/174 (12%)
Query: 24 PFHVITHRQHNLFFDGAGSELSSRRAI-LRLRFYGDDERCVVSLKAKAVLVDGVSRVEED 82
P HV ++H L + G EL++ ++ L+ ++ ++ L KA+ +D +
Sbjct: 33 PEHVKAIKEHGLRANYNGEELTAHLSVELQSEISSKEKTDLIILFTKAMQLDKML----- 87
Query: 83 EEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGL 142
+D+ P I DE K+ + + I E V N F+G N GL
Sbjct: 88 -QDIKPLI-----DEHTKVLCLLNGIGHEDTIEKYVSKNNIFIG--------NTMWTAGL 133
Query: 143 KLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGIDYSYSVASKFAIFR 196
+ F G++ E++ S EE + L E L E+G++ YS ++I+R
Sbjct: 134 EGPGKAKLFGDGSV-ELQNLISGEEETAKKLAEILSESGLNAKYSNNIHYSIYR 186
>HD11_XENLA (Q91695) Probable histone deacetylase 1-1 (HD1)
(Maternally-expressed histone deacetylase) (HDM) (AB21)
Length = 480
Score = 29.6 bits (65), Expect = 4.8
Identities = 30/96 (31%), Positives = 43/96 (44%), Gaps = 14/96 (14%)
Query: 80 EEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNVYDW 139
EEDE+D D +I D+ RI EEF ++ G GG KNV N
Sbjct: 395 EEDEDDPDKRISIRSSDK---------RIA--CDEEFS---DSEDEGEGGRKNVANFKKV 440
Query: 140 KGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
K +K E ++ D + E E + ++KR+ EE
Sbjct: 441 KRVKTEEEKEGEDKKDVKEEEKAKDEKTDSKRVKEE 476
>MCM4_MOUSE (P49717) DNA replication licensing factor MCM4 (CDC21
homolog) (P1-CDC21)
Length = 862
Score = 29.3 bits (64), Expect = 6.3
Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 12/111 (10%)
Query: 77 SRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRNV 136
S + +EE LD + +D + S IM R+ EE +V + + R +
Sbjct: 672 SEEQVEEEFLDMAVLKDYI------AYAHSTIMPRLSEEASQALIEAYVNMRKIGSSRGM 725
Query: 137 YDWKGLKLE----VDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGID 183
+LE + E H F + E+ D EEAKR+ E LK++ D
Sbjct: 726 VSAYPRQLESLIRLAEAHAK--VRFSNKVEAIDVEEAKRLHREALKQSATD 774
>HDRB_METTM (Q50755) CoB--CoM heterodisulfide reductase subunit B
(EC 1.8.98.1)
Length = 302
Score = 29.3 bits (64), Expect = 6.3
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 153 FGTLFEIECESSDPEEAKRILEEFLKENGIDYSYSV 188
FG+LFE + EE K + E LKE G +Y V
Sbjct: 82 FGSLFETNHLLKEDEEMKAKINEILKETGREYKGEV 117
>FRYA_SHIFL (Q83QP3) Putative phosphoenolpyruvate-protein
phosphotransferase fryA (EC 2.7.3.9) (Phosphotransferase
system, enzyme I)
Length = 831
Score = 29.3 bits (64), Expect = 6.3
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 4/34 (11%)
Query: 154 GTLFEIECE----SSDPEEAKRILEEFLKENGID 183
GTLF C SD E+A+R+LEE++ ID
Sbjct: 56 GTLFNDSCSLNISGSDEEQARRVLEEYILVRFID 89
>FLIG_THEMA (Q9WY63) Flagellar motor switch protein fliG
Length = 335
Score = 29.3 bits (64), Expect = 6.3
Identities = 23/66 (34%), Positives = 33/66 (49%), Gaps = 15/66 (22%)
Query: 150 HFDFGTLFEIECESSD-----PEEAKRILEEFL---------KENGIDYSYSVASK-FAI 194
H D T+ ++ E ++ PEE K++LEEFL E GI+Y+ V K F
Sbjct: 30 HLDEETVEQLVVEIANIGRVTPEEKKQVLEEFLSLAKAKEMISEGGIEYAKKVLEKAFGP 89
Query: 195 FRAGKL 200
RA K+
Sbjct: 90 ERARKI 95
>DCXR_MOUSE (Q91X52) L-xylulose reductase (EC 1.1.1.10) (XR)
(Dicarbonyl/L-xylulose reductase)
Length = 244
Score = 29.3 bits (64), Expect = 6.3
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 3/56 (5%)
Query: 44 LSSRRAILRLRFYGDDERCVVSLKAKAVLVDGVSRVEEDEEDL---DPKIGRDCVD 96
L+ RRA++ G V++LKA V VSR ED +DL P + CVD
Sbjct: 5 LAGRRALVTGAGKGIGRSTVLALKAAGAQVVAVSRTREDLDDLVRECPGVEPVCVD 60
>DCXR_CAVPO (Q920N9) L-xylulose reductase (EC 1.1.1.10) (XR)
(Dicarbonyl/L-xylulose reductase) (Protein P26h)
Length = 244
Score = 29.3 bits (64), Expect = 6.3
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 3/56 (5%)
Query: 44 LSSRRAILRLRFYGDDERCVVSLKAKAVLVDGVSRVEEDEEDL---DPKIGRDCVD 96
L+ RRA++ G V++LKA V VSR ED +DL P + CVD
Sbjct: 5 LAGRRALVTGAGKGIGRSTVLALKAAGAQVVAVSRTREDLDDLVRECPGVEPVCVD 60
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.141 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,546,340
Number of Sequences: 164201
Number of extensions: 1080859
Number of successful extensions: 2837
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 2834
Number of HSP's gapped (non-prelim): 19
length of query: 200
length of database: 59,974,054
effective HSP length: 105
effective length of query: 95
effective length of database: 42,732,949
effective search space: 4059630155
effective search space used: 4059630155
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144477.10