BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144474.2 - phase: 0
(201 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PR2_PETCR (P27538) Pathogenesis-related protein 2 33 0.44
A2BP_HUMAN (Q9NWB1) Ataxin 2-binding protein 1 33 0.44
YCF1_SPIOL (Q9M3J5) Hypothetical 56.9 kDa protein ycf1 32 1.3
SLA2_BACAN (P94217) S-layer protein EA1 precursor 32 1.3
MU5B_HUMAN (Q9HC84) Mucin 5B precursor (Mucin 5 subtype B, trach... 31 2.2
RBM9_HUMAN (O43251) RNA-binding protein 9 (RNA binding motif pro... 30 2.9
NUSG_AQUAE (O67757) Transcription antitermination protein nusG 30 2.9
MGP3_MYCPN (Q50341) Mgp-operon protein 3 precursor (ORF-3 protei... 30 2.9
A2BP_MOUSE (Q9JJ43) Ataxin 2-binding protein 1 30 2.9
SLAP_LACHE (P38059) S-layer protein precursor (Surface layer pro... 30 3.7
PIR_MOUSE (Q9D711) Pirin 30 4.9
HS7S_PEA (Q02028) Stromal 70 kDa heat shock-related protein, chl... 30 4.9
HIS1_SCHPO (P40373) ATP phosphoribosyltransferase (EC 2.4.2.17) ... 30 4.9
Y099_PYRHO (O57823) Hypothetical UPF0097 protein PH0099 29 6.4
GSPD_AERHY (P31780) General secretion pathway protein D precursor 29 6.4
DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostat... 29 6.4
Y033_THETN (Q8R6I7) Hypothetical UPF0236 protein TTE0033/TTE0744... 29 8.3
SYG_CHLPN (Q9Z6W0) Glycyl-tRNA synthetase (EC 6.1.1.14) (GlyRS) ... 29 8.3
SYFB_CLOTE (Q891T8) Phenylalanyl-tRNA synthetase beta chain (EC ... 29 8.3
>PR2_PETCR (P27538) Pathogenesis-related protein 2
Length = 158
Score = 33.1 bits (74), Expect = 0.44
Identities = 26/92 (28%), Positives = 46/92 (49%), Gaps = 8/92 (8%)
Query: 90 KIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGK-KYGVVTFGGV 148
++A +VP T K ++ M +P + +P+ I +I +GG VT G V
Sbjct: 9 EVASSVPAQTIYKGFLLDMDNIIPKV------LPQAIKSIEIISGDGGAGTIKKVTLGEV 62
Query: 149 ASDEVVKEKVEKLRLCLEKDGFKVI-GDFLLG 179
+ VVK++++++ K + +I GD LLG
Sbjct: 63 SQFTVVKQRIDEIDAEALKYSYSIIEGDLLLG 94
>A2BP_HUMAN (Q9NWB1) Ataxin 2-binding protein 1
Length = 397
Score = 33.1 bits (74), Expect = 0.44
Identities = 31/146 (21%), Positives = 52/146 (35%), Gaps = 19/146 (13%)
Query: 13 PKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKTEK 72
P+Y T + + Y P+ T G D G+PQ
Sbjct: 50 PEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQADDAAPTDGQPQ------ 103
Query: 73 ISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVI 132
T P ENKS +++ ++ + +F P + + K +D ++
Sbjct: 104 ---TQPSENTENKSQPKRLHVS----------NIPFRFRDPDLRQMFGQFGKILDVEIIF 150
Query: 133 REEGGKKYGVVTFGGVASDEVVKEKV 158
E G K +G VTF A + +EK+
Sbjct: 151 NERGSKGFGFVTFENSADADRAREKL 176
>YCF1_SPIOL (Q9M3J5) Hypothetical 56.9 kDa protein ycf1
Length = 481
Score = 31.6 bits (70), Expect = 1.3
Identities = 26/88 (29%), Positives = 38/88 (42%), Gaps = 2/88 (2%)
Query: 52 FKVLVDYIGIFGKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFT 111
F V Y+G P T K + T I +E EK + T T ++ + T +
Sbjct: 224 FITCVYYLGRMPSPIFTNKLKQMLETNEIEEETNLEIEKTSETKE--TKQEEEGFTEEDP 281
Query: 112 LPSMYLKVEEVPKPIDERVVIREEGGKK 139
PS++ + +E P IDE IR G K
Sbjct: 282 SPSLFSEEKEDPDKIDETEKIRVNGKDK 309
>SLA2_BACAN (P94217) S-layer protein EA1 precursor
Length = 862
Score = 31.6 bits (70), Expect = 1.3
Identities = 36/137 (26%), Positives = 54/137 (39%), Gaps = 12/137 (8%)
Query: 21 TQNYVIRKYAP--SLVAEIT-----YDPSTF--KGDKDGGFKVLVDYIGIFGKPQNTKTE 71
+QN V+ + A LVA I DP + K G V+ +YI + T T
Sbjct: 433 SQNLVVGEKASLNKLVATIAGEDKVVDPGSISIKSSNHGIISVVNNYITAEAAGEATLTI 492
Query: 72 KISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVV 131
K+ T + + + S K+ V V N V TLP ++ ++ P
Sbjct: 493 KVGDVTKDVKFKVTTDSRKL---VSVKANPDKLQVVQNKTLPVTFVTTDQYGDPFGANTA 549
Query: 132 IREEGGKKYGVVTFGGV 148
+E K GVV GG+
Sbjct: 550 AIKEVLPKTGVVAEGGL 566
>MU5B_HUMAN (Q9HC84) Mucin 5B precursor (Mucin 5 subtype B,
tracheobronchial) (High molecular weight salivary mucin
MG1) (Sublingual gland mucin)
Length = 5703
Score = 30.8 bits (68), Expect = 2.2
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 1/54 (1%)
Query: 65 PQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFT-LPSMYL 117
P T + + T +K SSS + A T+PV+T+ K FT +PS L
Sbjct: 2993 PPKVLTSQATTPTATSSKATSSSSPRTATTLPVLTSTATKSTATSFTPIPSSTL 3046
Score = 29.6 bits (65), Expect = 4.9
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 76 TTPVIT--KENKSSSEKIAMTVPVVTNEKNKMVTMQFT-LPSMYL 117
TTP T K SSS + A T+PV+T+ K FT +PS L
Sbjct: 4655 TTPAATSSKATSSSSPRTATTLPVLTSTATKSTATSFTPIPSSTL 4699
>RBM9_HUMAN (O43251) RNA-binding protein 9 (RNA binding motif
protein 9) (Hexaribonucleotide binding protein 2)
(Repressor of tamoxifen transcriptional activity)
Length = 390
Score = 30.4 bits (67), Expect = 2.9
Identities = 18/76 (23%), Positives = 34/76 (44%), Gaps = 2/76 (2%)
Query: 83 ENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYGV 142
+ +SS + + P + N + +F P + + K +D ++ E G K +G
Sbjct: 107 QTQSSENSESKSTPKRLHVSN--IPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGF 164
Query: 143 VTFGGVASDEVVKEKV 158
VTF A + +EK+
Sbjct: 165 VTFENSADADRAREKL 180
>NUSG_AQUAE (O67757) Transcription antitermination protein nusG
Length = 248
Score = 30.4 bits (67), Expect = 2.9
Identities = 19/62 (30%), Positives = 30/62 (47%)
Query: 96 PVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYGVVTFGGVASDEVVK 155
P NE + + L + V+EV P +E+VVIR +G +KY + G V+
Sbjct: 20 PGKENEAKENLLKVLELEGLKDLVDEVIVPAEEKVVIRAQGKEKYRLSLKGNARDISVLG 79
Query: 156 EK 157
+K
Sbjct: 80 KK 81
>MGP3_MYCPN (Q50341) Mgp-operon protein 3 precursor (ORF-3 protein)
(Mgp3)
Length = 1218
Score = 30.4 bits (67), Expect = 2.9
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 4/60 (6%)
Query: 47 DKDGGFKVLVDYIGIFGKPQN---TKTEKISMTTPVI-TKENKSSSEKIAMTVPVVTNEK 102
D DGG+K LV+ P N T + + TPV K ++E++ T PV +++K
Sbjct: 608 DSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQK 667
>A2BP_MOUSE (Q9JJ43) Ataxin 2-binding protein 1
Length = 396
Score = 30.4 bits (67), Expect = 2.9
Identities = 20/89 (22%), Positives = 38/89 (42%), Gaps = 10/89 (11%)
Query: 70 TEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDER 129
T+ T P E+KS +++ ++ + +F P + + K +D
Sbjct: 97 TDGQPQTQPSENTESKSQPKRLHVS----------NIPFRFRDPDLRQMFGQFGKILDVE 146
Query: 130 VVIREEGGKKYGVVTFGGVASDEVVKEKV 158
++ E G K +G VTF A + +EK+
Sbjct: 147 IIFNERGSKGFGFVTFENSADADRAREKL 175
>SLAP_LACHE (P38059) S-layer protein precursor (Surface layer
protein)
Length = 439
Score = 30.0 bits (66), Expect = 3.7
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 4/77 (5%)
Query: 24 YVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKTEKISMTTPVITKE 83
Y +Y + +T+D T D K+ V +G F P +T T + T+ K
Sbjct: 233 YFAAQYDKKQLTNVTFDTETAVKDALKAQKIEVSSVGYFKAP-HTFTVNVKATS---NKN 288
Query: 84 NKSSSEKIAMTVPVVTN 100
KS++ + +TVP V +
Sbjct: 289 GKSATLPVTVTVPNVAD 305
>PIR_MOUSE (Q9D711) Pirin
Length = 290
Score = 29.6 bits (65), Expect = 4.9
Identities = 24/95 (25%), Positives = 39/95 (40%), Gaps = 5/95 (5%)
Query: 49 DGGFKVLVDYIGIFGKPQNTKTEKISMTTPVITKENKSSSEKI-AMTVPVVTNEKNKMVT 107
+GG D+ G GK + ++ ++ E S E + + V KMV
Sbjct: 69 EGGSMAHEDFCGHVGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRRSEKMVA 128
Query: 108 MQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYGV 142
Q+ LK EE+PKP + V + G+ G+
Sbjct: 129 PQY----QELKSEEIPKPTKDGVTVAVISGEALGI 159
>HS7S_PEA (Q02028) Stromal 70 kDa heat shock-related protein,
chloroplast precursor
Length = 706
Score = 29.6 bits (65), Expect = 4.9
Identities = 27/132 (20%), Positives = 51/132 (38%), Gaps = 11/132 (8%)
Query: 63 GKPQNTKTEKISMTTPVITKENKSSSEKIAMTVP--VVTNEKNKMVTMQFTLPSMYLKVE 120
GKP + TTP + K+ + V N +N +++ + +V+
Sbjct: 88 GKPTIITNAEGQRTTPSVVAYTKNGDRLVGQIAKRQAVVNPENTFFSVKRFIGRKMSEVD 147
Query: 121 EVPKPIDERVVIREEGGKKYGVVTFG-GVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLG 179
E K + RV+ + G K G A++E+ + + K L D K + D
Sbjct: 148 EESKQVSYRVIRDDNGNVKLDCPAIGKSFAAEEISAQVLRK----LVDDASKFLND---- 199
Query: 180 RYNPPAITIPMF 191
+ +T+P +
Sbjct: 200 KVTKAVVTVPAY 211
>HIS1_SCHPO (P40373) ATP phosphoribosyltransferase (EC 2.4.2.17)
(ATP-PRTase) (ATP-PRT)
Length = 310
Score = 29.6 bits (65), Expect = 4.9
Identities = 24/96 (25%), Positives = 47/96 (48%), Gaps = 11/96 (11%)
Query: 71 EKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERV 130
E + T+ V+ + + SSE + ++T + ++ Q+ L + + E +P V
Sbjct: 199 ETVMSTSAVLVRSSNCSSELEPLLQTIITRIRGYIIAQQYVLVNYNVNREHLP------V 252
Query: 131 VIREEGGKKYGVVTF----GGVA-SDEVVKEKVEKL 161
V++ GK+ +T G VA S VVK++V ++
Sbjct: 253 VLKITPGKRAPTITTLDEPGWVAVSSMVVKKEVAQV 288
>Y099_PYRHO (O57823) Hypothetical UPF0097 protein PH0099
Length = 184
Score = 29.3 bits (64), Expect = 6.4
Identities = 19/68 (27%), Positives = 34/68 (49%), Gaps = 8/68 (11%)
Query: 118 KVEEVPKPIDERVVIREEGGKKYGV--------VTFGGVASDEVVKEKVEKLRLCLEKDG 169
+++ + K I E+ E K GV V + G+ +DE+++E ++ L K G
Sbjct: 56 EIKNILKKIAEKYKKHEVKVKGIGVFPNPNYIRVIWAGIENDEIIREMAREIEDELAKLG 115
Query: 170 FKVIGDFL 177
FK G+F+
Sbjct: 116 FKKEGNFV 123
>GSPD_AERHY (P31780) General secretion pathway protein D precursor
Length = 678
Score = 29.3 bits (64), Expect = 6.4
Identities = 14/37 (37%), Positives = 23/37 (61%), Gaps = 1/37 (2%)
Query: 67 NTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKN 103
NTK++ +S T ++T +NK +S + VPV T +N
Sbjct: 440 NTKSDILS-TPSIVTMDNKEASFNVGQEVPVQTGTQN 475
>DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostate
DLG) (Discs large protein P-dlg)
Length = 1809
Score = 29.3 bits (64), Expect = 6.4
Identities = 28/119 (23%), Positives = 52/119 (43%), Gaps = 16/119 (13%)
Query: 81 TKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSM----YLKVEEVPKPIDERVVIREEG 136
T + S S+ A T+P + + + P + ++KV++ +P+ +V E+G
Sbjct: 1201 TLSSCSQSQTSASTLPRIAVNPASLGERRKDRPYVEEPRHVKVQKGSEPLGISIVSGEKG 1260
Query: 137 GKKYGVVTFGGVA-------SDEVVKEKVEKLRLCLEKDGFKVIGD-----FLLGRYNP 183
G VT G +A D++++ LR E+ +IG +L +YNP
Sbjct: 1261 GIYVSKVTVGSIAHQAGLEYGDQLLEFNGINLRSATEQQARLIIGQQCDTITILAQYNP 1319
>Y033_THETN (Q8R6I7) Hypothetical UPF0236 protein
TTE0033/TTE0744/TTE0838/TTE0852/TTE1082/
TTE1247/TTE1519/TTE1678/TTE1739/TTE1823/TTE2212
Length = 467
Score = 28.9 bits (63), Expect = 8.3
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Query: 27 RKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQN-TKTEKISMTTPVITKENK 85
+K +++ +I Y+ + +K +DG + LVD G+ + K KI + I + +
Sbjct: 82 KKRLTTVLGDIEYERTYYKSREDGRYTYLVDDALEIGRHERIEKGVKIKLVENAIEESYE 141
Query: 86 SSSEK 90
SSEK
Sbjct: 142 KSSEK 146
>SYG_CHLPN (Q9Z6W0) Glycyl-tRNA synthetase (EC 6.1.1.14) (GlyRS)
[Includes: Glycyl-tRNA synthetase alpha chain
(Glycine--tRNA ligase alpha chain); Glycyl-tRNA
synthetase beta chain (Glycine--tRNA ligase beta chain)]
Length = 1010
Score = 28.9 bits (63), Expect = 8.3
Identities = 17/53 (32%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Query: 12 TPKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGK 64
T K ++ TT N ++K SL +T P GD++ FK ++D F K
Sbjct: 897 TEKLAVITTTHNR-LKKILSSLKLSMTSSPIEVLGDRESNFKQVLDAFPGFPK 948
>SYFB_CLOTE (Q891T8) Phenylalanyl-tRNA synthetase beta chain (EC
6.1.1.20) (Phenylalanine--tRNA ligase beta chain)
(PheRS)
Length = 791
Score = 28.9 bits (63), Expect = 8.3
Identities = 16/70 (22%), Positives = 34/70 (47%)
Query: 103 NKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLR 162
NK+V + + + +++V + IDE V+ ++G + + G + EV ++ E +
Sbjct: 277 NKIVIEKGKVGEKFATLDKVERTIDESVLCIKDGDRAVALAGIMGGLNSEVKEDTKEIIL 336
Query: 163 LCLEKDGFKV 172
C DG +
Sbjct: 337 ECANFDGTNI 346
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.137 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,786,811
Number of Sequences: 164201
Number of extensions: 1085098
Number of successful extensions: 3157
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 3145
Number of HSP's gapped (non-prelim): 25
length of query: 201
length of database: 59,974,054
effective HSP length: 105
effective length of query: 96
effective length of database: 42,732,949
effective search space: 4102363104
effective search space used: 4102363104
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144474.2