BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.5 - phase: 0 /pseudo
(125 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HIK2_HUMAN (Q9H2X6) Homeodomain-interacting protein kinase 2 (EC... 32 0.25
BIM1_YEAST (P40013) BIM1 protein 32 0.25
TWST_DROME (P10627) Twist protein 32 0.33
HIK2_MOUSE (Q9QZR5) Homeodomain-interacting protein kinase 2 (EC... 32 0.33
SPEN_DROME (Q8SX83) Split ends protein 30 0.73
PLO1_SCHPO (P50528) Serine/threonine-protein kinase plo1 (EC 2.7... 30 0.95
CCD5_MOUSE (Q8BHX1) Coiled-coil domain containing protein 5 30 0.95
FLUG_EMENI (P38094) Protein fluG 30 1.2
CCD5_HUMAN (Q96CS2) Coiled-coil domain containing protein 5 (Enh... 30 1.2
NBEA_DROME (Q9W4E2) Neurobeachin protein (Rugose protein) (A-kin... 29 2.1
CCD5_RAT (Q9R0A8) Coiled-coil domain containing protein 5 29 2.1
ATPG_SPIOL (P05435) ATP synthase gamma chain, chloroplast precur... 29 2.1
MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myos... 28 2.8
CBID_RALSO (Q8XS59) Putative cobalt-precorrin-6A synthase [deace... 28 2.8
ATP1_ARATH (Q01908) ATP synthase gamma chain 1, chloroplast prec... 28 2.8
ZSW6_MOUSE (Q80TB7) Zinc finger SWIM domain containing protein 6... 28 3.6
ZSW6_HUMAN (Q9HCJ5) Zinc finger SWIM domain containing protein 6... 28 3.6
ITK_HUMAN (Q08881) Tyrosine-protein kinase ITK/TSK (EC 2.7.1.112... 28 3.6
YD76_MYCPN (P75405) Hypothetical protein MPN376 (A19_orf1140) 28 4.7
UB49_HUMAN (Q70CQ1) Ubiquitin carboxyl-terminal hydrolase 49 (EC... 28 4.7
>HIK2_HUMAN (Q9H2X6) Homeodomain-interacting protein kinase 2 (EC
2.7.1.-)
Length = 1198
Score = 32.0 bits (71), Expect = 0.25
Identities = 22/84 (26%), Positives = 41/84 (48%)
Query: 8 RQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQ 67
RQ P + + R+ S +N +T +S++A+ + Q S+ V+ + M HSSPA
Sbjct: 792 RQQPTSTTSSRKSKQHQSSVRNVSTCEVSSSQAISSPQRSKRVKENTPPRCAMVHSSPAC 851
Query: 68 AKLVKLPKNLLAKVSTVKNTQQVL 91
+ V +A +T + +Q +
Sbjct: 852 STSVTCGWGDVASSTTRERQRQTI 875
>BIM1_YEAST (P40013) BIM1 protein
Length = 344
Score = 32.0 bits (71), Expect = 0.25
Identities = 32/138 (23%), Positives = 58/138 (41%), Gaps = 14/138 (10%)
Query: 1 MKKMEQRRQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQT----------- 49
+KK R +D R +RP+ T N+AT + A++ S T
Sbjct: 102 LKKHWIRHKDESVYDPDARRKYRPIITNNSATKPRTVSNPTTAKRSSSTGTGSAMSGGLA 161
Query: 50 VQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHME--- 106
+ +S ++ + +S Q +LV + L T+ + + +EQ +S+L+ E
Sbjct: 162 TRHSSLGINGSRKTSVTQGQLVAIQAELTKSQETIGSLNEEIEQYKGTVSTLEIEREFYF 221
Query: 107 NGLQNVPQLKTVVQLLAN 124
N L+++ L Q L N
Sbjct: 222 NKLRDIEILVHTTQDLIN 239
>TWST_DROME (P10627) Twist protein
Length = 490
Score = 31.6 bits (70), Expect = 0.33
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 8/94 (8%)
Query: 17 RRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKN 76
RRR +P T+ + A V E+ Q QS ++ +Q P LP +
Sbjct: 344 RRRLKRKPSKTEETDEFSNQRVMANVRER--QRTQSLNDAFKSLQQIIPT------LPSD 395
Query: 77 LLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQ 110
L+K+ T+K + ++ L R++SS D + L+
Sbjct: 396 KLSKIQTLKLATRYIDFLCRMLSSSDISLLKALE 429
>HIK2_MOUSE (Q9QZR5) Homeodomain-interacting protein kinase 2 (EC
2.7.1.-) (Nuclear body associated kinase 1) (Sialophorin
tail associated nuclear serine/threonine kinase)
Length = 1196
Score = 31.6 bits (70), Expect = 0.33
Identities = 22/84 (26%), Positives = 40/84 (47%)
Query: 8 RQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQ 67
RQ P + + R+ S +N +T S++A+ + Q S+ V+ + M HSSPA
Sbjct: 792 RQQPTSTTSSRKSKQHQSSVRNVSTCEVTSSQAISSPQRSKRVKENTPPRCAMVHSSPAC 851
Query: 68 AKLVKLPKNLLAKVSTVKNTQQVL 91
+ V +A +T + +Q +
Sbjct: 852 STSVTCGWGDVASSTTRERQRQTI 875
>SPEN_DROME (Q8SX83) Split ends protein
Length = 5560
Score = 30.4 bits (67), Expect = 0.73
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 3/49 (6%)
Query: 10 DPPTAANRRRPLWRPMSTQNAA---TGGDNSAKAVVAEQISQTVQSTSN 55
+PPT + ++PL +P+ T A TG SA +V+ +S + S SN
Sbjct: 3510 EPPTISKLQQPLVQPVQTVLPAPHSTGSGISANSVINLDLSNVISSCSN 3558
>PLO1_SCHPO (P50528) Serine/threonine-protein kinase plo1 (EC
2.7.1.37)
Length = 683
Score = 30.0 bits (66), Expect = 0.95
Identities = 20/73 (27%), Positives = 33/73 (44%)
Query: 34 GDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQ 93
G N +KAV E + STSN + MQH + ++ N + + + + + + V+
Sbjct: 574 GQNLSKAVQDESFEKPKNSTSNTMLFMQHYLRTRQAIMFRLSNGIFQFNFLDHRKVVISS 633
Query: 94 LPRVISSLDAHME 106
R I LD E
Sbjct: 634 TARKIIVLDKERE 646
>CCD5_MOUSE (Q8BHX1) Coiled-coil domain containing protein 5
Length = 278
Score = 30.0 bits (66), Expect = 0.95
Identities = 20/64 (31%), Positives = 34/64 (52%)
Query: 53 TSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNV 112
TS+L S + +L KL KNL A + K ++ L++ +S+ A +++ LQN+
Sbjct: 123 TSDLFRTKSKSEEMKLELGKLEKNLTATLVLEKCLREDLKKAELQLSAEKAKVDSRLQNM 182
Query: 113 PQLK 116
LK
Sbjct: 183 DFLK 186
>FLUG_EMENI (P38094) Protein fluG
Length = 865
Score = 29.6 bits (65), Expect = 1.2
Identities = 22/89 (24%), Positives = 43/89 (47%), Gaps = 7/89 (7%)
Query: 11 PPTAANRRRPLWRPMSTQNAATGGDNSAKAVVA--EQISQTVQSTSNLLHLMQ---HSSP 65
P T + W P T ++ + + ++ E+I++T+ S +HL Q S+P
Sbjct: 572 PTTDPSTGEEDWAPSVTNHSWSQMTRETRRMLPLLEEIAETLASIG--IHLQQFHAESAP 629
Query: 66 AQAKLVKLPKNLLAKVSTVKNTQQVLEQL 94
Q + + P N +A V T+ ++QV+ +
Sbjct: 630 GQFEFILPPDNPVAAVDTLIKSRQVIANI 658
>CCD5_HUMAN (Q96CS2) Coiled-coil domain containing protein 5
(Enhancer of invasion-cluster) (HEI-C)
Length = 278
Score = 29.6 bits (65), Expect = 1.2
Identities = 20/64 (31%), Positives = 33/64 (51%)
Query: 53 TSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNV 112
TS+L S + +L KL KNL A + K Q+ +++ +S+ A ++N QN+
Sbjct: 123 TSDLFRTKSKSEEIKIELEKLEKNLTATLVLEKCLQEDVKKAELHLSTERAKVDNRRQNM 182
Query: 113 PQLK 116
LK
Sbjct: 183 DFLK 186
>NBEA_DROME (Q9W4E2) Neurobeachin protein (Rugose protein) (A-kinase
anchor protein 550) (AKAP 550) (dAKAP550)
Length = 3584
Score = 28.9 bits (63), Expect = 2.1
Identities = 23/79 (29%), Positives = 39/79 (49%), Gaps = 4/79 (5%)
Query: 35 DNSAKAVVAEQISQTVQSTSNLL-HLMQHSSPAQAKLVKLPKNLLAKVSTVKN---TQQV 90
D++ KAV AEQ +QT + ++ SPA + VK + +T KN + +
Sbjct: 1565 DDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLL 1624
Query: 91 LEQLPRVISSLDAHMENGL 109
+EQ+ +V+ DA G+
Sbjct: 1625 VEQVQQVLQEDDAQQSAGM 1643
>CCD5_RAT (Q9R0A8) Coiled-coil domain containing protein 5
Length = 278
Score = 28.9 bits (63), Expect = 2.1
Identities = 21/64 (32%), Positives = 32/64 (49%)
Query: 53 TSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNV 112
TS+L S + +L KL KNL A + K ++ L++ +S+ A E LQN+
Sbjct: 123 TSDLFRTKSKSEEIKLELGKLEKNLTATLVLEKCLREDLKKADVHLSAERAKAEGRLQNM 182
Query: 113 PQLK 116
LK
Sbjct: 183 DFLK 186
>ATPG_SPIOL (P05435) ATP synthase gamma chain, chloroplast precursor
(EC 3.6.3.14)
Length = 364
Score = 28.9 bits (63), Expect = 2.1
Identities = 20/80 (25%), Positives = 42/80 (52%), Gaps = 4/80 (5%)
Query: 47 SQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHME 106
+Q+ Q+ N + ++P Q ++ L ++ +VKNTQ++ E + V ++ +
Sbjct: 20 TQSTQAPPNNATTLPTTNPIQCANLR---ELRDRIGSVKNTQKITEAMKLVAAAKVRRAQ 76
Query: 107 NGLQN-VPQLKTVVQLLANM 125
+ N P +T+V++L NM
Sbjct: 77 EAVVNGRPFSETLVEVLYNM 96
>MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myosin
heavy chain) (NMMHC)
Length = 1959
Score = 28.5 bits (62), Expect = 2.8
Identities = 17/68 (25%), Positives = 37/68 (54%), Gaps = 3/68 (4%)
Query: 39 KAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVI 98
K +AE++++ N+ L+ S +K +KL K+ A S +++TQ++L++ R+
Sbjct: 1268 KTELAERVNKLQVELDNVTGLLNQSD---SKSIKLAKDFSALESQLQDTQELLQEETRLK 1324
Query: 99 SSLDAHME 106
S ++
Sbjct: 1325 LSFSTKLK 1332
>CBID_RALSO (Q8XS59) Putative cobalt-precorrin-6A synthase
[deacetylating] (EC 2.1.1.-)
Length = 383
Score = 28.5 bits (62), Expect = 2.8
Identities = 14/52 (26%), Positives = 28/52 (52%), Gaps = 1/52 (1%)
Query: 30 AATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKV 81
AA + A + EQI +T + N++ ++QH AQA ++ + + A++
Sbjct: 302 AAVAAELGVAAALVEQI-KTANTVENVIQILQHQPGAQAFWTEIEQRIAARM 352
>ATP1_ARATH (Q01908) ATP synthase gamma chain 1, chloroplast
precursor (EC 3.6.3.14)
Length = 373
Score = 28.5 bits (62), Expect = 2.8
Identities = 19/63 (30%), Positives = 35/63 (55%), Gaps = 5/63 (7%)
Query: 64 SPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQN-VPQLKTVVQLL 122
SP QA L + L ++ +VKNTQ++ E + V ++ + + N P +T+V++L
Sbjct: 47 SPLQASL----RELRDRIDSVKNTQKITEAMKLVAAAKVRRAQEAVVNGRPFSETLVEVL 102
Query: 123 ANM 125
N+
Sbjct: 103 YNI 105
>ZSW6_MOUSE (Q80TB7) Zinc finger SWIM domain containing protein 6
(Fragment)
Length = 1017
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/57 (31%), Positives = 25/57 (43%)
Query: 26 STQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVS 82
ST A GD V E I + + S+S++ L Q + + LP L KVS
Sbjct: 641 STMLTAAKGDVRRLETVLESIQKNIHSSSHIFKLAQDAFKIATLMDSLPDITLLKVS 697
>ZSW6_HUMAN (Q9HCJ5) Zinc finger SWIM domain containing protein 6
(Fragment)
Length = 743
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/57 (31%), Positives = 25/57 (43%)
Query: 26 STQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVS 82
ST A GD V E I + + S+S++ L Q + + LP L KVS
Sbjct: 367 STMLTAAKGDVRRLETVLESIQKNIHSSSHIFKLAQDAFKIATLMDSLPDITLLKVS 423
>ITK_HUMAN (Q08881) Tyrosine-protein kinase ITK/TSK (EC 2.7.1.112)
(T-cell-specific kinase) (Tyrosine-protein kinase Lyk)
(Kinase EMT)
Length = 620
Score = 28.1 bits (61), Expect = 3.6
Identities = 11/25 (44%), Positives = 14/25 (56%)
Query: 3 KMEQRRQDPPTAANRRRPLWRPMST 27
K ++ PPT + RRPLW P T
Sbjct: 150 KNASKKPLPPTPEDNRRPLWEPEET 174
>YD76_MYCPN (P75405) Hypothetical protein MPN376 (A19_orf1140)
Length = 1140
Score = 27.7 bits (60), Expect = 4.7
Identities = 14/53 (26%), Positives = 27/53 (50%)
Query: 41 VVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQ 93
+V+E+ +S N+ + ++P Q+ ++KLP ++ K S V EQ
Sbjct: 945 LVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQ 997
>UB49_HUMAN (Q70CQ1) Ubiquitin carboxyl-terminal hydrolase 49 (EC
3.1.2.15) (Ubiquitin thiolesterase 49)
(Ubiquitin-specific processing protease 49)
(Deubiquitinating enzyme 49)
Length = 688
Score = 27.7 bits (60), Expect = 4.7
Identities = 25/93 (26%), Positives = 40/93 (42%), Gaps = 21/93 (22%)
Query: 3 KMEQRRQDPPT------AANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNL 56
K+EQRRQ+ A RRR + R + + A+T SA+ L
Sbjct: 168 KLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARL---------------L 212
Query: 57 LHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQ 89
LH + + PA ++ LP + +T+K +Q
Sbjct: 213 LHTPRDAGPAASRPAALPTSRRVPAATLKLRRQ 245
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.122 0.327
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,002,928
Number of Sequences: 164201
Number of extensions: 361504
Number of successful extensions: 1256
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 1238
Number of HSP's gapped (non-prelim): 49
length of query: 125
length of database: 59,974,054
effective HSP length: 101
effective length of query: 24
effective length of database: 43,389,753
effective search space: 1041354072
effective search space used: 1041354072
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144430.5