BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143339.8 + phase: 0 /pseudo
(518 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250... 52 3e-06
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 52 3e-06
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains... 44 0.001
PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type... 44 0.001
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 41 0.007
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 41 0.007
POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type... 34 1.0
PLSB_PHAVU (Q43822) Glycerol-3-phosphate acyltransferase, chloro... 33 1.4
COAS_MOUSE (Q9DBL7) Bifunctional coenzyme A synthase (CoA syntha... 33 2.3
PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type... 32 3.0
PLSX_CHLCV (Q821X8) Fatty acid/phospholipid synthesis protein plsX 32 4.0
PLSB_SPIOL (Q43869) Glycerol-3-phosphate acyltransferase, chloro... 32 4.0
COAS_HUMAN (Q13057) Bifunctional coenzyme A synthase (CoA syntha... 32 5.2
Y307_RHOPA (P61415) Hypothetical UPF0314 protein RPA0307 31 8.8
>MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250
(ORF102)
Length = 122
Score = 52.4 bits (124), Expect = 3e-06
Identities = 29/77 (37%), Positives = 44/77 (56%), Gaps = 1/77 (1%)
Query: 1 ICTATMYVLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSI 60
+C + ++NG+P RGLR+GDPLS +LF+L E L+ + + E G +
Sbjct: 5 VCHFYLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRV 64
Query: 61 GEHDSMVISHLQFADDT 77
++S I+HL FADDT
Sbjct: 65 -SNNSPRINHLLFADDT 80
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 52.4 bits (124), Expect = 3e-06
Identities = 58/230 (25%), Positives = 101/230 (43%), Gaps = 37/230 (16%)
Query: 5 TMYVLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGEH- 63
T +++NG FPL G R+G PLS LF + E L + ++ E G IG
Sbjct: 637 TANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEE 693
Query: 64 -------DSMVISHLQFADDT--LLVGVKSWANVRALRAVLLLLR*CRSGLKMNFHKSL- 113
D M++ D T LL +K ++NV SG K+N HKS+
Sbjct: 694 IKLSLFADDMIVYLENTRDSTTKLLEVIKEYSNV--------------SGYKINTHKSVA 739
Query: 114 LVGVNVSESWLNEATTMLGCKVGKVP--FLYLGLSIGGDPRRLL--FWESVVDRVKKRLS 169
+ N +++ E T VP YLG+ + D + L +E++ + + ++
Sbjct: 740 FIYTNNNQA---EKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAEDVN 796
Query: 170 GWRSKFLSFGGRLVLLKVFLSSLHVYALSF--FRAPSGIISTLESLFINF 217
W++ S+ GR+ ++K+ + +Y + +AP LE + ++F
Sbjct: 797 KWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHF 846
>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1300
Score = 43.9 bits (102), Expect = 0.001
Identities = 49/216 (22%), Positives = 93/216 (42%), Gaps = 15/216 (6%)
Query: 8 VLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGEHDSMV 67
+ VNG PL+ G R+G PLS +LF + E L ++ EI G IG+ + +
Sbjct: 667 IKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEI---KGIQIGKEEVKI 723
Query: 68 ISHLQFADDTLLVGVKSWANVRALRAVLLLLR*CRS--GLKMNFHKSLLVGVNVSESWLN 125
D ++V + N + R +L L+ G K+N +KS+ ++
Sbjct: 724 ----SLLADDMIVYISDPKN--STRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEK 777
Query: 126 EATTMLGCKVGKVPFLYLGLSIGGDPRRLL--FWESVVDRVKKRLSGWRSKFLSFGGRLV 183
E + YLG+++ + + L ++S+ +K+ L W+ S+ GR+
Sbjct: 778 EIRETTPFSIVTNNIKYLGVTLTKEVKDLYDKNFKSLKKEIKEDLRRWKDLPCSWIGRIN 837
Query: 184 LLKVFLSSLHVYALSF--FRAPSGIISTLESLFINF 217
++K+ + +Y + + P+ + LE F
Sbjct: 838 IVKMAILPKAIYRFNAIPIKIPTQFFNELEGAICKF 873
>PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 711
Score = 43.9 bits (102), Expect = 0.001
Identities = 38/109 (34%), Positives = 51/109 (45%), Gaps = 9/109 (8%)
Query: 12 GSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGEHDSMVISHL 71
GS T + + RG+++GDPLS FLF + L +QS I G +IGE I L
Sbjct: 189 GSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPGI----GGTIGEEK---IPVL 241
Query: 72 QFADDTLLVGVKSWANVRALRAVLLLLR*CRSGLKMNFHKSLLVGVNVS 120
FADD LL+ L V R G+ +N KS+ + V S
Sbjct: 242 AFADDLLLLEDNDVLLPTTLATVANFFR--LRGMSLNAKKSVSISVAAS 288
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein
(ORF 2)
Length = 1308
Score = 41.2 bits (95), Expect = 0.007
Identities = 57/223 (25%), Positives = 98/223 (43%), Gaps = 14/223 (6%)
Query: 3 TATMYVLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGE 62
+A V +N S T RG+R+G PLS L+ LA E +++ + +G + E
Sbjct: 630 SAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRKRL-----TGLVLKE 684
Query: 63 HDSMVISHLQFADDTLLVGVKSWANVRALRAVLLLLR*CRSGLKMNFHKS--LLVGVNVS 120
D V+ +ADD +LV RA + S ++N+ KS LL G ++
Sbjct: 685 PDMRVVLS-AYADDVILVAQDLVDLERAQECQEVYA--AASSARINWSKSSGLLEG-SLK 740
Query: 121 ESWLNEATTMLGCKVGKVPFLYLGLSIGGDPRRLLFWESVVDRVKKRLSGWR--SKFLSF 178
+L A + + + +L + LS P F E + + V RL W+ +K LS
Sbjct: 741 VDFLPPAFRDISWESKIIKYLGVYLSAEEYPVSQNFIE-LEECVLTRLGKWKGFAKVLSM 799
Query: 179 GGRLVLLKVFLSSLHVYALSFFRAPSGIISTLESLFINFFLVG 221
GR +++ ++S Y L I+ ++ ++F +G
Sbjct: 800 RGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLLDFLWIG 842
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 41.2 bits (95), Expect = 0.007
Identities = 46/217 (21%), Positives = 94/217 (43%), Gaps = 11/217 (5%)
Query: 5 TMYVLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGEHD 64
T +++NG PL+ G R+G PLS L + E L ++ EI G +G+ +
Sbjct: 637 TANIILNGQKLEAPPLKTGTRQGCPLSPLLPNIVLEVLARAIRQEKEI---KGIQLGKEE 693
Query: 65 SMVISHLQFADDTLLVGVKSWANVRALRAVLLLLR*CRSGLKMNFHKSLLVGVNVSESWL 124
+ FADD ++ + + L ++ SG K+N KS +
Sbjct: 694 ---VKLSLFADDMIVYLENPIVSAQNLLKLISNFSKV-SGYKINVQKSQAFLYTNNRQTE 749
Query: 125 NEATTMLGCKVGKVPFLYLGLSIGGDPRRLL--FWESVVDRVKKRLSGWRSKFLSFGGRL 182
++ + L + YLG+ + D + L ++ +++ +K+ + W++ S+ GR+
Sbjct: 750 SQIMSELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLNEIKEDTNKWKNIPCSWVGRI 809
Query: 183 VLLKVFLSSLHVYALSF--FRAPSGIISTLESLFINF 217
++K+ + +Y + + P + LE + F
Sbjct: 810 NIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKF 846
>POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type II
retrotransposable element R2DM [Contains: Protease (EC
3.4.23.-); Reverse transcriptase (EC 2.7.7.49);
Endonuclease]
Length = 1057
Score = 33.9 bits (76), Expect = 1.0
Identities = 35/110 (31%), Positives = 51/110 (45%), Gaps = 17/110 (15%)
Query: 11 NGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSIGEHDSMVISH 70
+G + EF RG+++GDPLS LF L + L + S + G +G + + +
Sbjct: 526 DGWSSEEFVPARGVKQGDPLSPILFNLVMDRLLRTLPSEI------GAKVG---NAITNA 576
Query: 71 LQFADDTLLVGVKSWANVRALRAVLL---LLR*CRSGLKMNFHKSLLVGV 117
FADD +L +A R VLL L GLK+N K VG+
Sbjct: 577 AAFADDLVL-----FAETRMGLQVLLDKTLDFLSIVGLKLNADKCFTVGI 621
>PLSB_PHAVU (Q43822) Glycerol-3-phosphate acyltransferase,
chloroplast precursor (EC 2.3.1.15) (GPAT)
Length = 461
Score = 33.5 bits (75), Expect = 1.4
Identities = 18/42 (42%), Positives = 25/42 (58%)
Query: 58 YSIGEHDSMVISHLQFADDTLLVGVKSWANVRALRAVLLLLR 99
+SIG + V S DD LV +K AN+RAL+ + +LLR
Sbjct: 272 FSIGRNLICVYSKKHMLDDPALVEMKRTANIRALKEMAMLLR 313
>COAS_MOUSE (Q9DBL7) Bifunctional coenzyme A synthase (CoA synthase)
[Includes: Phosphopantetheine adenylyltransferase (EC
2.7.7.3) (Pantetheine-phosphate adenylyltransferase)
(PPAT) (Dephospho-CoA pyrophosphorylase); Dephospho-CoA
kinase (EC 2.7.1.24) (D
Length = 563
Score = 32.7 bits (73), Expect = 2.3
Identities = 17/40 (42%), Positives = 20/40 (49%)
Query: 424 QMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLP 463
QM Q V+ S V+L +W V AW LLQ RLP
Sbjct: 517 QMSGQQLVEQSNVVLSTLWESHVTQSQVEKAWNLLQKRLP 556
>PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 606
Score = 32.3 bits (72), Expect = 3.0
Identities = 23/80 (28%), Positives = 37/80 (45%), Gaps = 7/80 (8%)
Query: 1 ICTATMYVLVNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQSMVEINIFSGYSI 60
I A ++V G T + + G+++GDPLS LF N+++ +V
Sbjct: 63 ITDAYTNIVVGGRTTNKIYIRNGVKQGDPLSPVLF-------NIVLDELVTRLNDEQPGA 115
Query: 61 GEHDSMVISHLQFADDTLLV 80
+ I+ L FADD LL+
Sbjct: 116 SMTPACKIASLAFADDLLLL 135
>PLSX_CHLCV (Q821X8) Fatty acid/phospholipid synthesis protein plsX
Length = 316
Score = 32.0 bits (71), Expect = 4.0
Identities = 15/47 (31%), Positives = 24/47 (50%)
Query: 147 IGGDPRRLLFWESVVDRVKKRLSGWRSKFLSFGGRLVLLKVFLSSLH 193
+GGD L+ WE ++D + R S F++F R V ++ S H
Sbjct: 10 MGGDHSPLVIWEVLIDVLNSRASNSHISFIAFASREVKEQILSSYTH 56
>PLSB_SPIOL (Q43869) Glycerol-3-phosphate acyltransferase,
chloroplast precursor (EC 2.3.1.15) (GPAT)
Length = 472
Score = 32.0 bits (71), Expect = 4.0
Identities = 18/42 (42%), Positives = 25/42 (58%)
Query: 58 YSIGEHDSMVISHLQFADDTLLVGVKSWANVRALRAVLLLLR 99
+S+G + V S DD LV VK AN R+L+ ++LLLR
Sbjct: 282 FSMGRNLLCVYSKKHMYDDPELVDVKKRANTRSLKELVLLLR 323
>COAS_HUMAN (Q13057) Bifunctional coenzyme A synthase (CoA synthase)
(NBP) (POV-2) [Includes: Phosphopantetheine
adenylyltransferase (EC 2.7.7.3) (Pantetheine-phosphate
adenylyltransferase) (PPAT) (Dephospho-CoA
pyrophosphorylase); Dephospho-CoA kinase (E
Length = 564
Score = 31.6 bits (70), Expect = 5.2
Identities = 15/40 (37%), Positives = 21/40 (52%)
Query: 424 QMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLP 463
QM Q V+ S V+L +W + + AW LLQ R+P
Sbjct: 518 QMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIP 557
>Y307_RHOPA (P61415) Hypothetical UPF0314 protein RPA0307
Length = 203
Score = 30.8 bits (68), Expect = 8.8
Identities = 14/43 (32%), Positives = 20/43 (45%), Gaps = 4/43 (9%)
Query: 471 HGVIPHSHDNQHCVIGCGDFETAQHLFRSCPFYVALWGQIRSW 513
HGV+ S ++QH D+ T H+ FY W +R W
Sbjct: 54 HGVVMSSENSQHLT----DWYTFSHIIHGFLFYAGTWLLLRRW 92
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.338 0.150 0.517
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,423,380
Number of Sequences: 164201
Number of extensions: 2434979
Number of successful extensions: 7156
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 7140
Number of HSP's gapped (non-prelim): 19
length of query: 518
length of database: 59,974,054
effective HSP length: 115
effective length of query: 403
effective length of database: 41,090,939
effective search space: 16559648417
effective search space used: 16559648417
T: 11
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 68 (30.8 bits)
Medicago: description of AC143339.8