BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143339.10 + phase: 0 /pseudo
(349 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SUT1_SCHPO (O14091) General alpha-glucoside permease 40 0.011
MATP_HUMAN (Q9UMX9) Membrane-associated transporter protein (AIM... 38 0.033
TSGA_SHIFL (P59269) TsgA protein homolog 35 0.28
NORA_STAAM (P21191) Quinolone resistance protein norA 35 0.28
TSGA_ECOLI (P60778) TsgA protein 34 0.48
TSGA_ECOL6 (P60779) TsgA protein homolog 34 0.48
TSGA_ECO57 (Q8X4S4) TsgA protein homolog 34 0.48
BMR2_BACSU (P39843) Multidrug resistance protein 2 (Multidrug-ef... 33 0.82
TSGA_SALTY (Q8ZLK7) TsgA protein homolog 33 1.1
TSGA_SALTI (Q8Z1Z8) TsgA protein homolog 33 1.1
SUL2_YEAST (Q12325) Sulfate permease 2 (High-affinity sulfate tr... 33 1.4
HUPK_RHILV (P28153) Hydrogenase expression/formation protein hupK 33 1.4
FTSK_THETN (Q8R5S4) DNA translocase ftsK 32 1.8
MOP_DESGI (Q46509) Aldehyde oxidoreductase (EC 1.2.-.-) (Molybde... 32 2.4
MATP_MOUSE (P58355) Membrane-associated transporter protein (AIM... 32 2.4
TXTP_CAEEL (P34519) Putative tricarboxylate transport protein, m... 32 3.1
SIA2_BRARE (Q7SYL3) Ubiquitin ligase SIAH2 (EC 6.3.2.-) (Seven i... 32 3.1
PMRA_STRPN (Q9ZEX9) Multi-drug resistance efflux pump pmrA 31 4.1
PTFB_ECOLI (P20966) PTS system, fructose-specific IIBC component... 30 9.1
LGT_MYCPN (P75547) Prolipoprotein diacylglyceryl transferase (EC... 30 9.1
>SUT1_SCHPO (O14091) General alpha-glucoside permease
Length = 553
Score = 39.7 bits (91), Expect = 0.011
Identities = 53/238 (22%), Positives = 100/238 (41%), Gaps = 31/238 (13%)
Query: 43 IAGIFLVYGIGQGF-TGSLFKLVTDYYWKDVQKLQPSTVQLYVGIYFIPWILKPIWGILT 101
+ G+ L + + G+ T LF L W + +++ +++PI GIL+
Sbjct: 43 LLGVQLTWSVELGYGTPYLFSLGLRKEWTSI---------IWIAGPLTGILIQPIAGILS 93
Query: 102 DAFPILGYRRRPYFVVAGVVGTVSATIVAVGGEV-------SVVAALMCFVGVSASLAIA 154
D RRRP+ + A ++GT S ++ ++ V+ + V + S+ +
Sbjct: 94 DRVNSRIGRRRPFMLCASLLGTFSLFLMGWAPDICLFIFSNEVLMKRVTIVLATISIYLL 153
Query: 155 DVTIDACIA-RNSIEVPGLAPDLQ----SLCGFCSGSGALLGYLASGF-------FVHHL 202
DV ++ +A S+ V + D Q S G G G +LGYL F++
Sbjct: 154 DVAVNVVMASTRSLIVDSVRSDQQHEANSWAGRMIGVGNVLGYLLGYLPLYRIFSFLNFT 213
Query: 203 GPQGSLGLMAVFPALTIVLGFV-IYEKRTTGLHNEKK-KGVVENVGTTIRSMYKTIKY 258
Q L ++ LT+ + + + E+R + +EK G + TT+R + +
Sbjct: 214 QLQVFCVLASISLVLTVTITTIFVSERRFPPVEHEKSVAGEIFEFFTTMRQSITALPF 271
>MATP_HUMAN (Q9UMX9) Membrane-associated transporter protein (AIM-1
protein) (Melanoma antigen AIM1)
Length = 530
Score = 38.1 bits (87), Expect = 0.033
Identities = 50/177 (28%), Positives = 70/177 (39%), Gaps = 34/177 (19%)
Query: 79 TVQLYVGIYFIPWILKPIWGILTDAFPILGY----------RRRPYFVVAGVVGTVSATI 128
+V L +Y I W L PI G L P++G RRRPY + GV+ V +
Sbjct: 62 SVGLPSSLYSIVWFLSPILGFLLQ--PVVGSASDHCRSRWGRRRPYILTLGVMMLVGMAL 119
Query: 129 VAVGGEVSVVAALMCFVGVSASLAIADVTIDACI---ARNSIEVPGLAPDLQSLC----- 180
G +VVAAL+ AI+ I + A + I+ P + L +C
Sbjct: 120 YLNG--ATVVAALIANPRRKLVWAISVTMIGVVLFDFAADFIDGP-IKAYLFDVCSHQDK 176
Query: 181 -------GFCSGSGALLGYLASGFFVHHLGPQGSLG----LMAVFPALTIVLGFVIY 226
+G G LGYL HL LG +M F AL + L F ++
Sbjct: 177 EKGLHYHALFTGFGGALGYLLGAIDWAHLELGRLLGTEFQVMFFFSALVLTLCFTVH 233
>TSGA_SHIFL (P59269) TsgA protein homolog
Length = 393
Score = 35.0 bits (79), Expect = 0.28
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + +LGFV
Sbjct: 326 SPKLVNFVLTCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFV 381
>NORA_STAAM (P21191) Quinolone resistance protein norA
Length = 388
Score = 35.0 bits (79), Expect = 0.28
Identities = 77/316 (24%), Positives = 129/316 (40%), Gaps = 54/316 (17%)
Query: 46 IFLVY-GIGQGFTGSLFKLVTDYYWKDVQKLQPSTVQLYVGIYFIP-WILKPIWGILTDA 103
IFL++ GIG L V Y KD+ L S + L V + + I+ P G L D
Sbjct: 12 IFLIFLGIG------LVIPVLPVYLKDLG-LTGSDLGLLVAAFALSQMIISPFGGTLADK 64
Query: 104 FPILGYRRRPYFVVAGVVGTVSATIVAVGGEVSVVAALMCFVGVSASLAIADVTIDACIA 163
LG ++ + ++ +VS + AVG SV+ G+SA + + VT IA
Sbjct: 65 ---LG--KKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGGMSAGMVMPGVT--GLIA 117
Query: 164 RNSIEVPGLAPDLQSLCGF-----CSGSGALLGYLASGFF--VHHLGP---QGSLGLMAV 213
++P Q F SG +LG GF V H P G+LG++A
Sbjct: 118 -------DISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFYFAGALGILAF 170
Query: 214 FPALTIVLGFVIYEKRTTGLHNEKKKGVVENVGTTIRSMYKTIKYPQAWKPSLYMFLSLA 273
++ ++ ++ KK + I + P + + L L+
Sbjct: 171 IMSIVLI--------------HDPKKSTTSGFQKLEPQLLTKINWKVFITP-VILTLVLS 215
Query: 274 LNVTTHEGHFYWYTDSKVGPAFSQEFVGIIYAIGAV-ASIIGVLIYHKYLKNYTFRNLVF 332
++ E + YT KV +S + + I G + ++ + + K++K F L F
Sbjct: 216 FGLSAFETLYSLYTADKVN--YSPKDISIAITGGGIFGALFQIYFFDKFMK--YFSELTF 271
Query: 333 YA-QLLYAISGLLDLM 347
A LLY++ L+ L+
Sbjct: 272 IAWSLLYSVVVLILLV 287
>TSGA_ECOLI (P60778) TsgA protein
Length = 393
Score = 34.3 bits (77), Expect = 0.48
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + +LGFV
Sbjct: 326 SPKLVNFVLTCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFV 381
>TSGA_ECOL6 (P60779) TsgA protein homolog
Length = 393
Score = 34.3 bits (77), Expect = 0.48
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + +LGFV
Sbjct: 326 SPKLVNFVLTCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFV 381
>TSGA_ECO57 (Q8X4S4) TsgA protein homolog
Length = 393
Score = 34.3 bits (77), Expect = 0.48
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + +LGFV
Sbjct: 326 SPKLVNFVLTCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFV 381
>BMR2_BACSU (P39843) Multidrug resistance protein 2
(Multidrug-efflux transporter 2)
Length = 400
Score = 33.5 bits (75), Expect = 0.82
Identities = 55/266 (20%), Positives = 110/266 (40%), Gaps = 25/266 (9%)
Query: 72 VQKLQPSTVQLYVGIYFIPWIL-KPIWGILTDAFPILGYRRRPYFVVAGVVGTVSATIVA 130
+ L ST+ V + I ++ P G D F R+ ++ ++ ++S I
Sbjct: 39 IMHLSGSTMGYLVAAFAISQLITSPFAGRWVDRFG-----RKKMIILGLLIFSLSELIFG 93
Query: 131 VGGEVSVVAALMCFVGVSASLAIADVT---IDACIARNSIEVPGLAPDLQSLCGFCSGSG 187
+G VS+ GVSA+ + VT D + + G S GF G G
Sbjct: 94 LGTHVSIFYFSRILGGVSAAFIMPAVTAYVADITTLKERSKAMGYVSAAIS-TGFIIGPG 152
Query: 188 ALLGYLASGFFVH-HLGPQGSLGLMAVFPALTIVLGFVIYEKRTTGLHNEKKKGVVENVG 246
A G +GF + ++ L+A ++ I+ + E+R + K+ ++++
Sbjct: 153 A--GGFIAGFGIRMPFFFASAIALIAAVTSVFILKESLSIEERHQLSSHTKESNFIKDLK 210
Query: 247 TTIRSMYKTIKYPQAWKPSLYMFLSLALNVTTHEGHFYWYTDSKVGPAFSQEFVGIIYAI 306
+I +Y + + +A ++ +E F ++D K G F+ + + I I
Sbjct: 211 RSIHPVYFI---------AFIIVFVMAFGLSAYETVFSLFSDHKFG--FTPKDIAAIITI 259
Query: 307 GA-VASIIGVLIYHKYLKNYTFRNLV 331
+ VA +I VL++ K + + ++
Sbjct: 260 SSIVAVVIQVLLFGKLVNKLGEKRMI 285
>TSGA_SALTY (Q8ZLK7) TsgA protein homolog
Length = 393
Score = 33.1 bits (74), Expect = 1.1
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + LGFV
Sbjct: 326 SPKLVNFILTCGTIGTMLTFVVTGPIVAHSGPQAALLTANGLYAVVFVMCFALGFV 381
>TSGA_SALTI (Q8Z1Z8) TsgA protein homolog
Length = 393
Score = 33.1 bits (74), Expect = 1.1
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 4/56 (7%)
Query: 173 APDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSL----GLMAVFPALTIVLGFV 224
+P L + C G +L ++ +G V H GPQ +L GL AV + LGFV
Sbjct: 326 SPKLVNFILTCGTIGTMLTFVVTGPIVAHSGPQAALLTANGLYAVVFVMCFALGFV 381
>SUL2_YEAST (Q12325) Sulfate permease 2 (High-affinity sulfate
transporter 2)
Length = 893
Score = 32.7 bits (73), Expect = 1.4
Identities = 26/98 (26%), Positives = 42/98 (42%), Gaps = 5/98 (5%)
Query: 114 YFVVAGVVGTVSATIVAVGGEVSVVAALMCFVGVSASLAIADVTIDACIARNSIEVPGLA 173
Y + + +G S + A +V + + + + + IADVT ++I P +A
Sbjct: 168 YGLYSSFIGAYSYSFFATSKDVCI--GPVAVMSLQTAKVIADVTAKYPDGDSAITGPVIA 225
Query: 174 PDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSLGLM 211
L LCG S A +G+L GF V + G M
Sbjct: 226 TTLALLCGIIS---AAVGFLRLGFLVELISLNAVAGFM 260
>HUPK_RHILV (P28153) Hydrogenase expression/formation protein hupK
Length = 370
Score = 32.7 bits (73), Expect = 1.4
Identities = 23/65 (35%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
Query: 131 VGGEVSVVAALMCFVGVSASLAIADVTIDACIARNSIEVPGLAPDLQSLCGFCSGSGALL 190
+G +V+V AL C V V A+ + + R E P LA + SLCGF A L
Sbjct: 10 IGIDVTVSRALACSVAVKANRPRGLTRM--FVGRQPEEAPVLAGQVFSLCGFAQSVAARL 67
Query: 191 GYLAS 195
LA+
Sbjct: 68 AVLAA 72
>FTSK_THETN (Q8R5S4) DNA translocase ftsK
Length = 709
Score = 32.3 bits (72), Expect = 1.8
Identities = 42/162 (25%), Positives = 70/162 (42%), Gaps = 19/162 (11%)
Query: 72 VQKLQPSTVQLYVGIYFIPWILKPIWGILTDAFPILGYRRRPYFVVAGVVGTVSATIVAV 131
+ K Q + VGI F+ + L + TD+ I+G + + G GT S I A
Sbjct: 2 IDKKQKPVKEEIVGIIFLAFTLISFLSLYTDSTGIIG--KHIGIFLKGFFGTGSYVISA- 58
Query: 132 GGEVSVVAALMC------FVGVSASLAIADVTIDACIARNSIEVPGLAPDLQS------L 179
+ +V ALM F+ + S+A+ + + I+ N + + D + +
Sbjct: 59 ---LLLVFALMFLFTNKNFIKLHRSMALLGLFL-MFISLNQLYYFPVLTDFKDYLSVAYI 114
Query: 180 CGFCSGSGALLGYLASGFFVHHLGPQGSLGLMAVFPALTIVL 221
G + G ++G L F V +G GS L+ F A+ IVL
Sbjct: 115 SGINNTGGGIIGSLIVYFLVKMVGIIGSYILLISFTAIFIVL 156
>MOP_DESGI (Q46509) Aldehyde oxidoreductase (EC 1.2.-.-) (Molybdenum
iron sulfur protein)
Length = 907
Score = 32.0 bits (71), Expect = 2.4
Identities = 17/53 (32%), Positives = 27/53 (50%)
Query: 144 FVGVSASLAIADVTIDACIARNSIEVPGLAPDLQSLCGFCSGSGALLGYLASG 196
FV + +A+VT+D + +++ L DL SLC + G + G LA G
Sbjct: 763 FVVYMYGVFMAEVTVDVATGQTTVDGMTLMADLGSLCNQLATDGQIYGGLAQG 815
>MATP_MOUSE (P58355) Membrane-associated transporter protein (AIM-1
protein) (Melanoma antigen AIM1) (Underwhite protein)
Length = 530
Score = 32.0 bits (71), Expect = 2.4
Identities = 45/175 (25%), Positives = 68/175 (38%), Gaps = 30/175 (17%)
Query: 79 TVQLYVGIYFIPWILKPIWGILTDAFPILGY----------RRRPYFVVAGVV------- 121
+V L +Y + W+L PI G L P++G RRRPY + ++
Sbjct: 62 SVGLPKSLYSMVWLLSPILGFLLQ--PVVGSASDHCRARWGRRRPYILTLAIMMLLGMAL 119
Query: 122 ----GTVSATIVAVGGEVSVVAALMCFVGVSASLAIADVTIDACIARNSIEVPGLAPDLQ 177
V + +VA + + A + VGV AD ID I +V +
Sbjct: 120 YLNGDAVVSALVANPRQKLIWAISITMVGVVLFDFSADF-IDGPIKAYLFDVCSHQDKEK 178
Query: 178 SL--CGFCSGSGALLGYLASGFFVHHLGPQGSLG----LMAVFPALTIVLGFVIY 226
L +G G LGY+ HL LG +M F AL ++L F+ +
Sbjct: 179 GLHYHALFTGFGGALGYILGAIDWVHLDLGRLLGTEFQVMFFFSALVLILCFITH 233
>TXTP_CAEEL (P34519) Putative tricarboxylate transport protein,
mitochondrial precursor (Citrate transport protein)
(CTP)
Length = 312
Score = 31.6 bits (70), Expect = 3.1
Identities = 31/118 (26%), Positives = 48/118 (40%), Gaps = 14/118 (11%)
Query: 202 LGPQGSLGLMAVFPALTIVLGFVIYEKRTTGLHNEKKKGVVENVGTTIRS-----MYKTI 256
LG S + AV P T+ + F+ GL K KG V VG +++ +YK +
Sbjct: 129 LGAGLSEAVFAVTPMETVKVKFI----HDQGLAQPKYKGFVHGVGCIVKAEGLGGIYKGV 184
Query: 257 KYPQAWKPSLYMFLSLALNVTTHEGHFYWYTDSKVGPAFSQEFVGIIYAIGAVASIIG 314
A + S + A+ E WY S+ VG++ A+ AS+ G
Sbjct: 185 TATMAKQGS-----NQAIRFFVMETLKDWYRGGDNTQPISKPIVGLMGAVAGAASVYG 237
>SIA2_BRARE (Q7SYL3) Ubiquitin ligase SIAH2 (EC 6.3.2.-) (Seven in
absentia homolog 2) (Siah-2)
Length = 331
Score = 31.6 bits (70), Expect = 3.1
Identities = 20/67 (29%), Positives = 30/67 (43%), Gaps = 2/67 (2%)
Query: 119 GVVGTVSATIVAVGGEVSVVAALMCFVGVSASLAIADVTIDACIARNSIEVPGLAPDLQS 178
G GT ++ A + AA GVS S+A + A +A +PG +P+L +
Sbjct: 26 GSGGTTASAAAAAAAAAAAAAAAAAAAGVSGSVAGSGTVPAAAVALPVAALPGQSPELTA 85
Query: 179 L--CGFC 183
L C C
Sbjct: 86 LFECPVC 92
>PMRA_STRPN (Q9ZEX9) Multi-drug resistance efflux pump pmrA
Length = 399
Score = 31.2 bits (69), Expect = 4.1
Identities = 54/232 (23%), Positives = 85/232 (36%), Gaps = 38/232 (16%)
Query: 53 GQGFTGSLFKLVTDYYWKDVQKLQPSTVQL--YVGIYFI-----PWILKPIWGILTDAFP 105
G TG+ LV + V+ L + Q+ Y G+ + PIWGIL D
Sbjct: 16 GNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADK-- 73
Query: 106 ILGYRRRPYFVVAGVVGTVSATIVAVGGEVSVVAALMCFVGVSASLAIADVTIDACIARN 165
Y R+P + AG+ T++ +A + + L GV A + A
Sbjct: 74 ---YGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGF------VPNATALI 124
Query: 166 SIEVPGLAPDLQSLCGFCSG---SGALLGYLASGFFVHHLGPQGSLGLMAVFPALTIVLG 222
+ +VP S G S +G L G GF G + L+ F L +L
Sbjct: 125 ASQVP--KEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAIL- 181
Query: 223 FVIYEKRTTGLHNEKKKGVVENVGTTIRSMYKTIKYPQAWKPSLYMFLSLAL 274
T E + V + + ++ ++KYP Y+ L+L L
Sbjct: 182 -------TICFIKEDFQPVAKEKAIPTKELFTSVKYP-------YLLLNLFL 219
>PTFB_ECOLI (P20966) PTS system, fructose-specific IIBC component
(EIIBC-Fru) (Fructose-permease IIBC component)
(Phosphotransferase enzyme II, BC component) (EC
2.7.1.69) (EII-Fru)
Length = 563
Score = 30.0 bits (66), Expect = 9.1
Identities = 16/57 (28%), Positives = 28/57 (49%)
Query: 169 VPGLAPDLQSLCGFCSGSGALLGYLASGFFVHHLGPQGSLGLMAVFPALTIVLGFVI 225
+P A D + C GAL G ++ + P G L ++ + A+T VLG+++
Sbjct: 479 IPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAITPVLGYLV 535
>LGT_MYCPN (P75547) Prolipoprotein diacylglyceryl transferase (EC
2.4.99.-)
Length = 389
Score = 30.0 bits (66), Expect = 9.1
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 285 WYTDSKVGPAFSQEFVGIIYAIGAVASIIGV---LIYHKYLKNYTFRNLVFYAQL 336
W T +GP F ++ GII AIG I+ LIY +++ +F +F A L
Sbjct: 11 WSTAFYLGPGFPIQWYGIIVAIGIAFGILMFVLKLIYFYKIQDNSFYFFIFIAVL 65
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.141 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,042,059
Number of Sequences: 164201
Number of extensions: 1775259
Number of successful extensions: 5557
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 5547
Number of HSP's gapped (non-prelim): 25
length of query: 349
length of database: 59,974,054
effective HSP length: 111
effective length of query: 238
effective length of database: 41,747,743
effective search space: 9935962834
effective search space used: 9935962834
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 66 (30.0 bits)
Medicago: description of AC143339.10