BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142394.3 - phase: 0
(321 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
IRS1_RAT (P35570) Insulin receptor substrate-1 (pp185) 35 0.33
IRS1_MOUSE (P35569) Insulin receptor substrate-1 35 0.33
YM53_MYCBO (P64956) Hypothetical protein Mb2253c 33 0.95
YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287 33 0.95
DCW1_ASHGO (Q75DG6) Mannan endo-1,6-alpha-mannosidase DCW1 precu... 32 1.6
PARC_CAUCR (O54478) Topoisomerase IV subunit A (EC 5.99.1.-) 32 2.8
HXT7_YEAST (P39004) High-affinity hexose transporter HXT6 30 6.2
HXT6_YEAST (P39003) High-affinity hexose transporter HXT6 30 6.2
RNH_ZYMMO (O69014) Ribonuclease H (EC 3.1.26.4) (RNase H) 30 8.1
DCDA_THEMA (Q9X1K5) Diaminopimelate decarboxylase (EC 4.1.1.20) ... 30 8.1
>IRS1_RAT (P35570) Insulin receptor substrate-1 (pp185)
Length = 1235
Score = 34.7 bits (78), Expect = 0.33
Identities = 36/130 (27%), Positives = 53/130 (40%), Gaps = 15/130 (11%)
Query: 108 YWNNNVEWRDFWATACHTIWNWRNKETHNDNYQRPLHAKNIIMIYVNDYHNAIAKFVIVT 167
Y+ N +WR + +I + N N + K+++ +Y D H AIA
Sbjct: 46 YYENEKKWRHKSSAPKRSI---PLESCFNINKRADSKNKHLVALYTRDEHFAIAA----- 97
Query: 168 SQPRHLEEVGWKEPAIRWVKINTDGACKDGSIAGCGGLIRGSEGEWLAG--FSKFLGKCD 225
E+ W + ++ N A DG+ GCGG GS G AG S G
Sbjct: 98 --DSEAEQDSWYQALLQLH--NRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGP 153
Query: 226 AFIAELWGVL 235
AF E+W V+
Sbjct: 154 AF-KEVWQVI 162
>IRS1_MOUSE (P35569) Insulin receptor substrate-1
Length = 1233
Score = 34.7 bits (78), Expect = 0.33
Identities = 36/130 (27%), Positives = 53/130 (40%), Gaps = 15/130 (11%)
Query: 108 YWNNNVEWRDFWATACHTIWNWRNKETHNDNYQRPLHAKNIIMIYVNDYHNAIAKFVIVT 167
Y+ N +WR + +I + N N + K+++ +Y D H AIA
Sbjct: 46 YYENEKKWRHKSSAPKRSI---PLESCFNINKRADSKNKHLVALYTRDEHFAIAA----- 97
Query: 168 SQPRHLEEVGWKEPAIRWVKINTDGACKDGSIAGCGGLIRGSEGEWLAG--FSKFLGKCD 225
E+ W + ++ N A DG+ GCGG GS G AG S G
Sbjct: 98 --DSEAEQDSWYQALLQLH--NRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGP 153
Query: 226 AFIAELWGVL 235
AF E+W V+
Sbjct: 154 AF-KEVWQVI 162
>YM53_MYCBO (P64956) Hypothetical protein Mb2253c
Length = 364
Score = 33.1 bits (74), Expect = 0.95
Identities = 33/133 (24%), Positives = 61/133 (45%), Gaps = 13/133 (9%)
Query: 186 VKINTDGACKDG-SIAGCGGLIRGSEGEWLAGFSK-FLGKCDAFIAELWGVLEGLRCAKR 243
V I DG + AG G ++ ++ + SK +G+ +AE G++ GL A +
Sbjct: 3 VVIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVK 62
Query: 244 MGFTAVELNVDSLVVVNIITSERESN--------ASGRSLVQKIRKLLQMEWKVKVKHSY 295
+G T + +DS +VV ++ + ++L + R+ + EW + +++Y
Sbjct: 63 LGATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALASQFRR-INYEWVPRARNTY 121
Query: 296 --REANRCADALA 306
R AN DA A
Sbjct: 122 ADRLANDAMDAAA 134
>YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287
Length = 364
Score = 33.1 bits (74), Expect = 0.95
Identities = 33/133 (24%), Positives = 61/133 (45%), Gaps = 13/133 (9%)
Query: 186 VKINTDGACKDG-SIAGCGGLIRGSEGEWLAGFSK-FLGKCDAFIAELWGVLEGLRCAKR 243
V I DG + AG G ++ ++ + SK +G+ +AE G++ GL A +
Sbjct: 3 VVIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVK 62
Query: 244 MGFTAVELNVDSLVVVNIITSERESN--------ASGRSLVQKIRKLLQMEWKVKVKHSY 295
+G T + +DS +VV ++ + ++L + R+ + EW + +++Y
Sbjct: 63 LGATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALASQFRR-INYEWVPRARNTY 121
Query: 296 --REANRCADALA 306
R AN DA A
Sbjct: 122 ADRLANDAMDAAA 134
>DCW1_ASHGO (Q75DG6) Mannan endo-1,6-alpha-mannosidase DCW1
precursor (EC 3.2.1.101) (Endo-alpha-1->6-D-mannanase
DCW1) (Defective cell wall 1)
Length = 451
Score = 32.3 bits (72), Expect = 1.6
Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 16/71 (22%)
Query: 179 KEPAIRWVKINTDGAC------KDGSIAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELW 232
+E +RW++ + GA +DG G LI G +G+W G + + +
Sbjct: 331 RETIVRWIRASAQGAAASCSGGRDGHTCGLNWLINGWDGKW--GLGEQMAALE------- 381
Query: 233 GVLEGLRCAKR 243
+++ LRC +R
Sbjct: 382 -IIQNLRCLER 391
>PARC_CAUCR (O54478) Topoisomerase IV subunit A (EC 5.99.1.-)
Length = 759
Score = 31.6 bits (70), Expect = 2.8
Identities = 21/83 (25%), Positives = 33/83 (39%), Gaps = 6/83 (7%)
Query: 44 KHKMGIGNPMCRFCHDEIESEIHVLRDCPKATALWLCVVDNAAR------TSFFEGDLSS 97
K + +GN FC + + ++ V+ D K L + R ++ EG L
Sbjct: 648 KQVLNVGNEGAAFCLEAVGDQLAVIGDNGKILIFPLEELPEMPRGKGVKLQAYREGGLRD 707
Query: 98 WIKFNLFTNVYWNNNVEWRDFWA 120
+ FN T YW + R WA
Sbjct: 708 GLSFNAETGAYWIDTAGRRRDWA 730
>HXT7_YEAST (P39004) High-affinity hexose transporter HXT6
Length = 570
Score = 30.4 bits (67), Expect = 6.2
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Query: 160 IAKFVIVTSQPRHLEEVGWKEPAIRWVKINTDGACKDGSI-AGCGGLIRGSEGEWLAG 216
I V PR+L EVG E A R + ++ A D S+ A ++ G E E LAG
Sbjct: 250 IGGMTFVPESPRYLAEVGKIEEAKRSIAVSNKVAVDDPSVLAEVEAVLAGVEAEKLAG 307
>HXT6_YEAST (P39003) High-affinity hexose transporter HXT6
Length = 570
Score = 30.4 bits (67), Expect = 6.2
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Query: 160 IAKFVIVTSQPRHLEEVGWKEPAIRWVKINTDGACKDGSI-AGCGGLIRGSEGEWLAG 216
I V PR+L EVG E A R + ++ A D S+ A ++ G E E LAG
Sbjct: 250 IGGMTFVPESPRYLAEVGKIEEAKRSIAVSNKVAVDDPSVLAEVEAVLAGVEAEKLAG 307
>RNH_ZYMMO (O69014) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 156
Score = 30.0 bits (66), Expect = 8.1
Identities = 27/79 (34%), Positives = 37/79 (46%), Gaps = 5/79 (6%)
Query: 186 VKINTDGACK-DGSIAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGVLEGLRCAKRM 244
V I TDGACK + G G L+R E S+ EL V+E L C K+
Sbjct: 11 VMIATDGACKGNPGFGGWGALLRYQGHEKAISGSE--NPTTNNRMELQAVIEALSCLKKP 68
Query: 245 GFTAVELNVDSLVVVNIIT 263
+EL+ DS V++ +T
Sbjct: 69 --CQIELSTDSKYVMDGLT 85
>DCDA_THEMA (Q9X1K5) Diaminopimelate decarboxylase (EC 4.1.1.20)
(DAP decarboxylase)
Length = 386
Score = 30.0 bits (66), Expect = 8.1
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 10/52 (19%)
Query: 4 NFDNDAQDEIWLKLWNIRAPERIKHFMSM------LYHGRLSTNLRKHKMGI 49
N D+ + EIW +L PE +++F+ + H +ST L+KHK GI
Sbjct: 109 NVDSFEEMEIWREL----NPEGVEYFIRVNPEVDAKTHPHISTGLKKHKFGI 156
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.137 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,651,062
Number of Sequences: 164201
Number of extensions: 1611534
Number of successful extensions: 3196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 3193
Number of HSP's gapped (non-prelim): 10
length of query: 321
length of database: 59,974,054
effective HSP length: 110
effective length of query: 211
effective length of database: 41,911,944
effective search space: 8843420184
effective search space used: 8843420184
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 66 (30.0 bits)
Medicago: description of AC142394.3