Medicago
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140850.3 + phase: 0 /pseudo
         (650 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YL52_CAEEL (P34431) Hypothetical protein F44E2.2 in chromosome III     61  1e-08
POLY_DROME (P10401) Retrovirus-related Pol polyprotein from tran...    58  7e-08
POL4_DROME (P10394) Retrovirus-related Pol polyprotein from tran...    57  1e-07
POL5_DROME (Q8I7P9) Retrovirus-related Pol polyprotein from tran...    55  4e-07
RRPO_OENBE (P31843) RNA-directed DNA polymerase homolog (Reverse...    54  1e-06
RT23_SCHPO (Q9UR07) Retrotransposable element Tf2 155 kDa protei...    54  2e-06
RT22_SCHPO (Q9C0R2) Retrotransposable element Tf2 155 kDa protei...    54  2e-06
RT21_SCHPO (Q05654) Retrotransposable element Tf2 155 kDa protei...    54  2e-06
POL2_DROME (P20825) Retrovirus-related Pol polyprotein from tran...    52  6e-06
POL3_DROME (P04323) Retrovirus-related Pol polyprotein from tran...    51  8e-06
POL_CAMVS (P03554) Enzymatic polyprotein [Contains: Aspartic pro...    45  8e-04
POL_CAMVE (Q02964) Enzymatic polyprotein [Contains: Aspartic pro...    44  0.001
POL_CAMVC (P03555) Enzymatic polyprotein [Contains: Aspartic pro...    44  0.001
POL_COYMV (P19199) Putative polyprotein [Contains: Coat protein;...    44  0.002
POL_SOCMV (P15629) Enzymatic polyprotein [Contains: Aspartic pro...    43  0.003
POL_CAMVD (P03556) Enzymatic polyprotein [Contains: Aspartic pro...    42  0.005
POL_SFV1 (P23074) Pol polyprotein [Contains: Protease (EC 3.4.23...    41  0.009
POL_CAMVN (Q00962) Enzymatic polyprotein [Contains: Aspartic pro...    41  0.011
POL_SFV3L (P27401) Pol polyprotein [Contains: Protease (EC 3.4.2...    40  0.015
POL_FOAMV (P14350) Pol polyprotein [Contains: Reverse transcript...    40  0.015

>YL52_CAEEL (P34431) Hypothetical protein F44E2.2 in chromosome III
          Length = 2186

 Score = 60.8 bits (146), Expect = 1e-08
 Identities = 26/75 (34%), Positives = 46/75 (60%)

Query: 104  WVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGY 163
            W + +V V KKDG +RMC+D+R +NK    +  PLP+I+  + + A  K+++  D  +G+
Sbjct: 978  WSSPVVLVKKKDGSIRMCIDYRKVNKVVKNNAHPLPNIEATLQSLAGKKLYTVFDMIAGF 1037

Query: 164  NLIKMSPEDREKTSF 178
              I +  + +E T+F
Sbjct: 1038 WQIPLDEKSKEITAF 1052


>POLY_DROME (P10401) Retrovirus-related Pol polyprotein from
           transposon gypsy [Contains: Reverse transcriptase (EC
           2.7.7.49); Endonuclease]
          Length = 1035

 Score = 58.2 bits (139), Expect = 7e-08
 Identities = 26/64 (40%), Positives = 43/64 (66%)

Query: 119 RMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSF 178
           R+ +DFR LN+ +  D +P+P I +++ N  ++K F+ +D  SGY+ I ++  DREKTSF
Sbjct: 238 RLVIDFRKLNEKTIPDRYPMPSIPMILANLGKAKFFTTLDLKSGYHQIYLAEHDREKTSF 297

Query: 179 ITHG 182
             +G
Sbjct: 298 SVNG 301


>POL4_DROME (P10394) Retrovirus-related Pol polyprotein from
           transposon 412 [Contains: Protease (EC 3.4.23.-);
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1237

 Score = 57.4 bits (137), Expect = 1e-07
 Identities = 31/97 (31%), Positives = 55/97 (55%), Gaps = 6/97 (6%)

Query: 90  RLMRVFLMTVEYPEWVANIVPVPKKDG------KVRMCVDFRDLNKASPKDNFPLPHIDV 143
           +L++  ++     ++ + ++ VPKK        K R+ +D+R +NK    D FPLP ID 
Sbjct: 336 KLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLLADKFPLPRIDD 395

Query: 144 LVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
           ++D   ++K FS +D  SG++ I++    R+ TSF T
Sbjct: 396 ILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFST 432


>POL5_DROME (Q8I7P9) Retrovirus-related Pol polyprotein from
           transposon opus [Contains: Protease (EC 3.4.23.-);
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1003

 Score = 55.5 bits (132), Expect = 4e-07
 Identities = 27/79 (34%), Positives = 43/79 (54%)

Query: 102 PEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFS 161
           P W+    P P  + + RM VDF+ LN  +  D +P+P I+  + +   +K F+ +D  S
Sbjct: 162 PIWIVPKKPKPNGEKQYRMVVDFKRLNTVTIPDTYPIPDINATLASLGNAKYFTTLDLTS 221

Query: 162 GYNLIKMSPEDREKTSFIT 180
           G++ I M   D  KT+F T
Sbjct: 222 GFHQIHMKESDIPKTAFST 240


>RRPO_OENBE (P31843) RNA-directed DNA polymerase homolog (Reverse
           transcriptase homolog)
          Length = 142

 Score = 54.3 bits (129), Expect = 1e-06
 Identities = 24/63 (38%), Positives = 41/63 (64%)

Query: 118 VRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTS 177
           +RMC+D+R L K + K+ +P+P +D L D  AQ+  F+ +D  SGY  ++++  D  KT+
Sbjct: 6   LRMCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKTT 65

Query: 178 FIT 180
            +T
Sbjct: 66  CVT 68


>RT23_SCHPO (Q9UR07) Retrotransposable element Tf2 155 kDa protein
           type 3
          Length = 1333

 Score = 53.5 bits (127), Expect = 2e-06
 Identities = 27/68 (39%), Positives = 42/68 (61%)

Query: 111 VPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSP 170
           VPKK+G +RM VD++ LNK    + +PLP I+ L+     S +F+ +D  S Y+LI++  
Sbjct: 455 VPKKEGTLRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRK 514

Query: 171 EDREKTSF 178
            D  K +F
Sbjct: 515 GDEHKLAF 522


>RT22_SCHPO (Q9C0R2) Retrotransposable element Tf2 155 kDa protein
           type 2
          Length = 1333

 Score = 53.5 bits (127), Expect = 2e-06
 Identities = 27/68 (39%), Positives = 42/68 (61%)

Query: 111 VPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSP 170
           VPKK+G +RM VD++ LNK    + +PLP I+ L+     S +F+ +D  S Y+LI++  
Sbjct: 455 VPKKEGTLRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRK 514

Query: 171 EDREKTSF 178
            D  K +F
Sbjct: 515 GDEHKLAF 522


>RT21_SCHPO (Q05654) Retrotransposable element Tf2 155 kDa protein
           type 1
          Length = 1333

 Score = 53.5 bits (127), Expect = 2e-06
 Identities = 27/68 (39%), Positives = 42/68 (61%)

Query: 111 VPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSP 170
           VPKK+G +RM VD++ LNK    + +PLP I+ L+     S +F+ +D  S Y+LI++  
Sbjct: 455 VPKKEGTLRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRK 514

Query: 171 EDREKTSF 178
            D  K +F
Sbjct: 515 GDEHKLAF 522


>POL2_DROME (P20825) Retrovirus-related Pol polyprotein from
           transposon 297 [Contains: Protease (EC 3.4.23.-);
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1059

 Score = 51.6 bits (122), Expect = 6e-06
 Identities = 24/79 (30%), Positives = 43/79 (54%)

Query: 102 PEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFS 161
           P WV    P      K R+ +D+R LN+ +  D +P+P++D ++    + + F+ +D   
Sbjct: 245 PTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAK 304

Query: 162 GYNLIKMSPEDREKTSFIT 180
           G++ I+M  E   KT+F T
Sbjct: 305 GFHQIEMDEESISKTAFST 323


>POL3_DROME (P04323) Retrovirus-related Pol polyprotein from
           transposon 17.6 [Contains: Protease (EC 3.4.23.-);
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1058

 Score = 51.2 bits (121), Expect = 8e-06
 Identities = 30/87 (34%), Positives = 47/87 (53%), Gaps = 11/87 (12%)

Query: 102 PEWVANIVPVPKKDG-----KVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSF 156
           P WV     VPKK       K R+ +D+R LN+ +  D  P+P++D ++    +   F+ 
Sbjct: 246 PIWV-----VPKKQDASGKQKFRIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTT 300

Query: 157 MDGFSGYNLIKMSPEDREKTSFIT-HG 182
           +D   G++ I+M PE   KT+F T HG
Sbjct: 301 IDLAKGFHQIEMDPESVSKTAFSTKHG 327


>POL_CAMVS (P03554) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 679

 Score = 44.7 bits (104), Expect = 8e-04
 Identities = 24/66 (36%), Positives = 38/66 (57%)

Query: 113 KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
           K+ GK RM V+++ +NKA+  D + LP+ D L+      K+FS  D  SG+  + +  E 
Sbjct: 295 KRRGKKRMVVNYKAMNKATVGDAYNLPNKDELLTLIRGKKIFSSFDCKSGFWQVLLDQES 354

Query: 173 REKTSF 178
           R  T+F
Sbjct: 355 RPLTAF 360


>POL_CAMVE (Q02964) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 679

 Score = 44.3 bits (103), Expect = 0.001
 Identities = 24/66 (36%), Positives = 38/66 (57%)

Query: 113 KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
           K+ GK RM V+++ +NKA+  D + LP+ D L+      K+FS  D  SG+  + +  E 
Sbjct: 295 KRRGKKRMVVNYKAMNKATIGDAYNLPNKDELLTLIRGKKIFSSFDCKSGFWQVLLDQES 354

Query: 173 REKTSF 178
           R  T+F
Sbjct: 355 RPLTAF 360


>POL_CAMVC (P03555) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 679

 Score = 44.3 bits (103), Expect = 0.001
 Identities = 24/66 (36%), Positives = 38/66 (57%)

Query: 113 KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
           K+ GK RM V+++ +NKA+  D + LP+ D L+      K+FS  D  SG+  + +  E 
Sbjct: 295 KRRGKKRMVVNYKAMNKATIGDAYNLPNKDELLTLIRGKKIFSSFDCKSGFWQVLLDQES 354

Query: 173 REKTSF 178
           R  T+F
Sbjct: 355 RPLTAF 360


>POL_COYMV (P19199) Putative polyprotein [Contains: Coat protein;
            Protease (EC 3.4.23.-); Reverse transcriptase (EC
            2.7.7.49); Ribonuclease H (EC 3.1.26.4)]
          Length = 1886

 Score = 43.5 bits (101), Expect = 0.002
 Identities = 21/67 (31%), Positives = 38/67 (56%)

Query: 113  KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
            +K GK RM  +++ LN+ +  D + LP I+ ++    +SK++S  D  SG+  + M  E 
Sbjct: 1458 EKKGKERMVFNYKLLNENTESDQYSLPGINTIISKVGRSKIYSKFDLKSGFWQVAMEEES 1517

Query: 173  REKTSFI 179
               T+F+
Sbjct: 1518 VPWTAFL 1524


>POL_SOCMV (P15629) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 692

 Score = 42.7 bits (99), Expect = 0.003
 Identities = 20/65 (30%), Positives = 38/65 (57%)

Query: 114 KDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDR 173
           K GK RM ++++ +N+A+  D++ LP  D +++    S  FS +D  SGY  +++    +
Sbjct: 253 KRGKRRMVINYKKMNEATIGDSYKLPRKDFILEKIKGSLWFSSLDAKSGYYQLRLHENTK 312

Query: 174 EKTSF 178
             T+F
Sbjct: 313 PLTAF 317


>POL_CAMVD (P03556) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 674

 Score = 42.0 bits (97), Expect = 0.005
 Identities = 23/66 (34%), Positives = 37/66 (55%)

Query: 113 KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
           K+ GK RM V+++ +NKA+  D +  P+ D L+      K+FS  D  SG+  + +  E 
Sbjct: 290 KRRGKKRMVVNYKAMNKATVGDAYNPPNKDELLTLIRGKKIFSSFDCKSGFWQVLLDQES 349

Query: 173 REKTSF 178
           R  T+F
Sbjct: 350 RPLTAF 355


>POL_SFV1 (P23074) Pol polyprotein [Contains: Protease (EC
           3.4.23.-); Reverse transcriptase/ribonuclease H (EC
           2.7.7.49) (EC 3.1.26.4) (RT); Integrase (IN)]
          Length = 1161

 Score = 41.2 bits (95), Expect = 0.009
 Identities = 23/75 (30%), Positives = 39/75 (51%)

Query: 108 IVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIK 167
           + PVPK DGK RM +D+R++NK  P       H   ++ +  + K  + +D  +G+    
Sbjct: 214 VYPVPKPDGKWRMVLDYREVNKTIPLIAAQNQHSAGILSSIYRGKYKTTLDLTNGFWAHP 273

Query: 168 MSPEDREKTSFITHG 182
           ++PE    T+F   G
Sbjct: 274 ITPESYWLTAFTWQG 288


>POL_CAMVN (Q00962) Enzymatic polyprotein [Contains: Aspartic
           protease (EC 3.4.23.-); Endonuclease; Reverse
           transcriptase (EC 2.7.7.49)]
          Length = 680

 Score = 40.8 bits (94), Expect = 0.011
 Identities = 22/63 (34%), Positives = 35/63 (54%)

Query: 116 GKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREK 175
           G  RM V+++ +NKA+  D + LP+ D L+      K+FS  D  SG+  + +  E R  
Sbjct: 299 GNKRMVVNYKAMNKATVGDAYNLPNKDELLTLIRGKKIFSSFDCKSGFWQVLLDQESRPL 358

Query: 176 TSF 178
           T+F
Sbjct: 359 TAF 361


>POL_SFV3L (P27401) Pol polyprotein [Contains: Protease (EC
           3.4.23.-); Reverse transcriptase/ribonuclease H (EC
           2.7.7.49) (EC 3.1.26.4) (RT); Integrase (IN)]
          Length = 1157

 Score = 40.4 bits (93), Expect = 0.015
 Identities = 22/71 (30%), Positives = 38/71 (52%)

Query: 108 IVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIK 167
           + PVPK DGK RM +D+R++NK  P       H   ++ +  + K  + +D  +G+    
Sbjct: 216 VYPVPKPDGKWRMVLDYREVNKTIPLIAAQNQHSAGILSSIFRGKYKTTLDLSNGFWAHS 275

Query: 168 MSPEDREKTSF 178
           ++PE    T+F
Sbjct: 276 ITPESYWLTAF 286


>POL_FOAMV (P14350) Pol polyprotein [Contains: Reverse
           transcriptase/ribonuclease H (EC 2.7.7.49) (EC 3.1.26.4)
           (RT); Integrase (IN)]
          Length = 886

 Score = 40.4 bits (93), Expect = 0.015
 Identities = 22/75 (29%), Positives = 38/75 (50%)

Query: 108 IVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIK 167
           + PVPK DG+ RM +D+R++NK  P       H   ++    + K  + +D  +G+    
Sbjct: 5   VYPVPKPDGRWRMVLDYREVNKTIPLTAAQNQHSAGILATIVRQKYKTTLDLANGFWAHP 64

Query: 168 MSPEDREKTSFITHG 182
           ++PE    T+F   G
Sbjct: 65  ITPESYWLTAFTWQG 79


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.347    0.151    0.514 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,630,760
Number of Sequences: 164201
Number of extensions: 2299662
Number of successful extensions: 7127
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 7099
Number of HSP's gapped (non-prelim): 35
length of query: 650
length of database: 59,974,054
effective HSP length: 117
effective length of query: 533
effective length of database: 40,762,537
effective search space: 21726432221
effective search space used: 21726432221
T: 11
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 69 (31.2 bits)


Medicago: description of AC140850.3