BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140774.9 - phase: 0 /pseudo
(174 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KRAF_DROME (P11346) Raf homolog serine/threonine-protein kinase ... 39 0.008
MLL2_HUMAN (O14686) Myeloid/lymphoid or mixed-lineage leukemia p... 33 0.43
104K_THEPA (P15711) 104 kDa microneme-rhoptry antigen 33 0.43
MLL3_MOUSE (Q8BRH4) Myeloid/lymphoid or mixed-lineage leukemia p... 32 0.96
MLL3_HUMAN (Q8NEZ4) Myeloid/lymphoid or mixed-lineage leukemia p... 32 0.96
AGRN_HUMAN (O00468) Agrin precursor 29 4.8
TAC2_HUMAN (O95359) Transforming acidic coiled-coil-containing p... 29 6.2
RAF1_CHICK (P05625) RAF proto-oncogene serine/threonine-protein ... 28 8.1
NCL1_CAEEL (P34611) B-box type zinc-finger protein ncl-1 28 8.1
KDGB_RAT (P49621) Diacylglycerol kinase, beta (EC 2.7.1.107) (Di... 28 8.1
>KRAF_DROME (P11346) Raf homolog serine/threonine-protein kinase
dRAF-1 (EC 2.7.1.37) (Pole-hole protein)
Length = 781
Score = 38.5 bits (88), Expect = 0.008
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
Query: 71 SHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFCALAPPAL-KAHPFHSQH 129
+H +H + L C GC+ F C QCN++ H CA P L + P S +
Sbjct: 261 THIKHQIIRKTFFSLVFCEGCRRL-LFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYY 319
Query: 130 AMLFHSKPDDKI 141
+L PD+ +
Sbjct: 320 QLLLAENPDNGV 331
>MLL2_HUMAN (O14686) Myeloid/lymphoid or mixed-lineage leukemia
protein 2 (ALL1-related protein)
Length = 5262
Score = 32.7 bits (73), Expect = 0.43
Identities = 17/46 (36%), Positives = 26/46 (55%), Gaps = 3/46 (6%)
Query: 83 PDLFICVGCKEYGS-GKRFVCQQCNYQLHDFCALAPPALKAHPFHS 127
P+ +C C++ G+ K VC+ C+ H FC L PP ++ P HS
Sbjct: 271 PECKVCQACRKPGNDSKMLVCETCDKGYHTFC-LKPP-MEELPAHS 314
>104K_THEPA (P15711) 104 kDa microneme-rhoptry antigen
Length = 924
Score = 32.7 bits (73), Expect = 0.43
Identities = 20/57 (35%), Positives = 26/57 (45%)
Query: 20 KSPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHP 76
KSP PS SK+ + +SPPP P+SP+ G +II S P P
Sbjct: 590 KSPRTASPTRRPSPKLPQLSKLPKSTSPRSPPPPTRPSSPERPEGTKIIKTSKPPSP 646
>MLL3_MOUSE (Q8BRH4) Myeloid/lymphoid or mixed-lineage leukemia
protein 3 homolog (Histone-lysine N-methyltransferase,
H3 lysine-4 specific MLL3) (EC 2.1.1.43)
Length = 4903
Score = 31.6 bits (70), Expect = 0.96
Identities = 15/43 (34%), Positives = 21/43 (47%), Gaps = 3/43 (6%)
Query: 83 PDLFICVGCKEYGS-GKRFVCQQCNYQLHDFCALAPPALKAHP 124
P+ +C CK+ G K VC C+ H FC P +K+ P
Sbjct: 385 PECKVCQNCKQSGEDSKMLVCDTCDKGYHTFC--LQPVMKSVP 425
>MLL3_HUMAN (Q8NEZ4) Myeloid/lymphoid or mixed-lineage leukemia
protein 3 homolog (Histone-lysine N-methyltransferase,
H3 lysine-4 specific MLL3) (EC 2.1.1.43) (Homologous to
ALR protein)
Length = 4911
Score = 31.6 bits (70), Expect = 0.96
Identities = 15/43 (34%), Positives = 21/43 (47%), Gaps = 3/43 (6%)
Query: 83 PDLFICVGCKEYGS-GKRFVCQQCNYQLHDFCALAPPALKAHP 124
P+ +C CK+ G K VC C+ H FC P +K+ P
Sbjct: 386 PECKVCQNCKQSGEDSKMLVCDTCDKGYHTFC--LQPVMKSVP 426
>AGRN_HUMAN (O00468) Agrin precursor
Length = 2045
Score = 29.3 bits (64), Expect = 4.8
Identities = 21/72 (29%), Positives = 28/72 (38%), Gaps = 7/72 (9%)
Query: 31 PSHDDQYYSKVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHPLSMVDLPDLFICVG 90
PSH Q +K T T + PP T+P + G PQ P D F
Sbjct: 1291 PSHTSQPVAKTTAAPTTRRPP----TTAPSRVPGRRP---PAPQQPPKPCDSQPCFHGGT 1343
Query: 91 CKEYGSGKRFVC 102
C+++ G F C
Sbjct: 1344 CQDWALGGGFTC 1355
>TAC2_HUMAN (O95359) Transforming acidic coiled-coil-containing
protein 2 (Anti Zuai-1) (AZU-1)
Length = 1026
Score = 28.9 bits (63), Expect = 6.2
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 6/49 (12%)
Query: 5 KQKQVEKKTNTFLLEKSPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPI 53
K+K+ KT+TF ++KSP + PS D T T ++PP I
Sbjct: 498 KKKKTPLKTDTFRVKKSPKRSPLSDPPSQDP------TPAATPETPPVI 540
>RAF1_CHICK (P05625) RAF proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (RAF-1) (C-RAF) (MIL proto-oncogene
serine/threonine-protein kinase) (C-MIL)
Length = 647
Score = 28.5 bits (62), Expect = 8.1
Identities = 25/114 (21%), Positives = 47/114 (40%), Gaps = 14/114 (12%)
Query: 53 IEFPTSPQLIFGEEII-----HFSHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNY 107
+++ T + GEE+ H H + L C C+++ F CQ C Y
Sbjct: 112 LDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNG-FRCQTCGY 170
Query: 108 QLHDFCALAPPALKAHPFHSQHAMLF-HSKPDDK-------IQMRRLWQTNKRV 153
+ H+ C+ P + + + +LF +S D + MRR+ ++ R+
Sbjct: 171 KFHEHCSTKVPTMCVDWSNIRQLLLFPNSNISDSGVPALPPLTMRRMRESVSRI 224
>NCL1_CAEEL (P34611) B-box type zinc-finger protein ncl-1
Length = 851
Score = 28.5 bits (62), Expect = 8.1
Identities = 13/29 (44%), Positives = 15/29 (50%), Gaps = 1/29 (3%)
Query: 88 CVGCKEYGSGKRFVCQQCNYQLHDFCALA 116
C GCK + F CQ CN L D C +A
Sbjct: 132 CSGCKSNETATSF-CQDCNANLCDNCTMA 159
>KDGB_RAT (P49621) Diacylglycerol kinase, beta (EC 2.7.1.107)
(Diglyceride kinase) (DGK-beta) (DAG kinase beta) (90
kDa diacylglycerol kinase)
Length = 801
Score = 28.5 bits (62), Expect = 8.1
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Query: 95 GSGKR-FVCQQCNYQLHDFCALAPPA 119
G GK+ C C Y +H+ CA APP+
Sbjct: 266 GVGKQGLCCSFCKYTVHERCARAPPS 291
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.136 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,106,823
Number of Sequences: 164201
Number of extensions: 916972
Number of successful extensions: 2824
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2818
Number of HSP's gapped (non-prelim): 19
length of query: 174
length of database: 59,974,054
effective HSP length: 103
effective length of query: 71
effective length of database: 43,061,351
effective search space: 3057355921
effective search space used: 3057355921
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC140774.9