BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140721.12 - phase: 0
(109 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PRIA_BACSU (P94461) Primosomal protein N' (Replication factor Y) 40 0.001
DNA1_CHLPN (Q9Z8M9) Chromosomal replication initiator protein dn... 30 1.3
BCS1_SCHPO (Q9P6Q3) Probable mitochondrial chaperone BCS1 (BCS1-... 30 1.3
KSGA_METJA (Q58435) Probable dimethyladenosine transferase (EC 2... 29 1.7
KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus... 29 1.7
Y7I9_ANASP (Q05067) Hypothetical ABC transporter ATP-binding pro... 29 2.2
KF3A_MOUSE (P28741) Kinesin-like protein KIF3A (Microtubule plus... 28 2.9
DYHB_CHLRE (Q39565) Dynein beta chain, flagellar outer arm 28 2.9
YJR1_YEAST (P46992) Hypothetical 43.0 kDa protein in CPS1-FPP1 i... 28 3.8
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 28 5.0
HMW2_MYCGE (P47460) Cytadherence high molecular weight protein 2... 28 5.0
DO2C_MOUSE (Q9ESN1) Double C2-like domain containing protein gam... 28 5.0
KLP1_CHLRE (P46870) Kinesin-like protein KLP1 27 6.5
KF3C_RAT (O55165) Kinesin-like protein KIF3C 27 6.5
KF3C_MOUSE (O35066) Kinesin-like protein KIF3C 27 6.5
KF3C_HUMAN (O14782) Kinesin-like protein KIF3C 27 6.5
GIT1_YEAST (P25346) Probable metabolite transport protein GIT1 27 6.5
DNA1_CHLMU (Q9PKE4) Chromosomal replication initiator protein dn... 27 6.5
TRPF_AQUAE (O67853) N-(5'-phosphoribosyl)anthranilate isomerase ... 27 8.5
PRIA_CHLPN (Q9Z6Y2) Primosomal protein N' (Replication factor Y) 27 8.5
>PRIA_BACSU (P94461) Primosomal protein N' (Replication factor Y)
Length = 805
Score = 39.7 bits (91), Expect = 0.001
Identities = 21/51 (41%), Positives = 30/51 (58%)
Query: 37 LMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKG 87
L LTDEQR + I ++ D+ F L+G G+GKT I+ S+ V +KG
Sbjct: 266 LPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKG 316
>DNA1_CHLPN (Q9Z8M9) Chromosomal replication initiator protein dnaA
1
Length = 460
Score = 29.6 bits (65), Expect = 1.3
Identities = 12/38 (31%), Positives = 26/38 (67%)
Query: 63 FLYGYGGTGKTFIWKTSLAVVRSKGLIVLNAASRVYRE 100
+L+G G+GKT + +++++V+R G +L +S ++ E
Sbjct: 145 YLFGPEGSGKTHLMQSAISVLRESGGKILYVSSDLFTE 182
>BCS1_SCHPO (Q9P6Q3) Probable mitochondrial chaperone BCS1
(BCS1-like protein)
Length = 449
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/71 (32%), Positives = 40/71 (55%), Gaps = 11/71 (15%)
Query: 32 QTDMLLMLTDEQRCVHDKIMDSKGFDDGGF-----FFLYGYGGTGKT-FIWKTSLAVVRS 85
++++ M+TD+ VHD + +S+ +D G + LYG G+GKT F++ +LA
Sbjct: 213 ESNVKKMITDD---VHDFLRNSQWYDTRGIPYRRGYLLYGPPGSGKTSFLY--ALAGELD 267
Query: 86 KGLIVLNAASR 96
+ VLN A +
Sbjct: 268 YDICVLNLAEK 278
>KSGA_METJA (Q58435) Probable dimethyladenosine transferase (EC
2.1.1.-) (S-adenosylmethionine-6-N',N'-adenosyl(rRNA)
dimethyltransferase) (16S rRNA dimethylase)
Length = 275
Score = 29.3 bits (64), Expect = 1.7
Identities = 14/39 (35%), Positives = 24/39 (60%), Gaps = 3/39 (7%)
Query: 15 KLIVD---ELNYNKDELTKTQTDMLLMLTDEQRCVHDKI 50
K ++D ELNYNKDE+ K D L ++ + +++K+
Sbjct: 212 KALIDSSKELNYNKDEMKKILEDFLNTNSEIKNLINEKV 250
>KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus
end-directed kinesin motor 3A)
Length = 702
Score = 29.3 bits (64), Expect = 1.7
Identities = 16/40 (40%), Positives = 21/40 (52%)
Query: 50 IMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGLI 89
I+DS G F YG GTGKTF + A+ +G+I
Sbjct: 84 IIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGII 123
>Y7I9_ANASP (Q05067) Hypothetical ABC transporter ATP-binding
protein all4389
Length = 335
Score = 28.9 bits (63), Expect = 2.2
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 10/71 (14%)
Query: 27 ELTKTQTDMLLMLTDEQRC------VHDKIMDSKGFD----DGGFFFLYGYGGTGKTFIW 76
+LT T ++M +D ++ ++ K++ KG G F L G G GKT +
Sbjct: 11 QLTTKNTPSVVMTSDLRKVYRTGFWMNQKVVSLKGCSLTVYQGETFGLLGPNGAGKTTLL 70
Query: 77 KTSLAVVRSKG 87
K L ++R G
Sbjct: 71 KLLLGIIRPSG 81
>KF3A_MOUSE (P28741) Kinesin-like protein KIF3A (Microtubule plus
end-directed kinesin motor 3A)
Length = 701
Score = 28.5 bits (62), Expect = 2.9
Identities = 17/40 (42%), Positives = 21/40 (52%)
Query: 50 IMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGLI 89
I+DS G F YG GTGKTF + AV +G+I
Sbjct: 84 IIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPGLRGVI 123
>DYHB_CHLRE (Q39565) Dynein beta chain, flagellar outer arm
Length = 4568
Score = 28.5 bits (62), Expect = 2.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Query: 63 FLYGYGGTGKTFIWKTSLAVVRSKG 87
FL G G GKT +W+T L S G
Sbjct: 2199 FLLGAAGCGKTAVWRTLLRAQNSSG 2223
>YJR1_YEAST (P46992) Hypothetical 43.0 kDa protein in CPS1-FPP1
intergenic region
Length = 396
Score = 28.1 bits (61), Expect = 3.8
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
Query: 34 DMLLMLTDEQRCVHDKIMDSK-GFDDG------GFFFLYGYGGTGKTFIWKTSLAVVRSK 86
D L+ +D++ ++ + K G+D G G YGYGGT K F+++ + K
Sbjct: 159 DGTLISSDQEYIIYSNVSCPKSGYDKGCGVYRSGIPAYYGYGGTTKMFLFEFEMPTETEK 218
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 27.7 bits (60), Expect = 5.0
Identities = 14/50 (28%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
Query: 37 LMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSK 86
+ L+DEQ+ + D +++ + F G GTGK+ + + + V++SK
Sbjct: 308 IFLSDEQKRILDMVVEQQHS-----IFFTGSAGTGKSVLLRKIIEVLKSK 352
>HMW2_MYCGE (P47460) Cytadherence high molecular weight protein 2
(Cytadherence accessory protein 2)
Length = 1805
Score = 27.7 bits (60), Expect = 5.0
Identities = 13/41 (31%), Positives = 22/41 (52%)
Query: 14 NKLIVDELNYNKDELTKTQTDMLLMLTDEQRCVHDKIMDSK 54
N+L L + ++E + Q+D LL L E + + KI + K
Sbjct: 356 NELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFK 396
>DO2C_MOUSE (Q9ESN1) Double C2-like domain containing protein gamma
(Doc2 gamma)
Length = 387
Score = 27.7 bits (60), Expect = 5.0
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Query: 38 MLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSL 80
+L RCVH MD+ G+ D F L+ + +GK +KTS+
Sbjct: 260 LLVGVLRCVHLAPMDANGYSD-PFVRLFLHPSSGKKSKYKTSV 301
>KLP1_CHLRE (P46870) Kinesin-like protein KLP1
Length = 776
Score = 27.3 bits (59), Expect = 6.5
Identities = 18/52 (34%), Positives = 23/52 (43%), Gaps = 1/52 (1%)
Query: 49 KIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGLIVLNAASRVYRE 100
+++DS G F YG G GKTF +GLI A V+RE
Sbjct: 74 EVVDSLMAGYHGTIFAYGQTGAGKTFTMSGGGTAYAHRGLIP-RAIHHVFRE 124
>KF3C_RAT (O55165) Kinesin-like protein KIF3C
Length = 796
Score = 27.3 bits (59), Expect = 6.5
Identities = 23/70 (32%), Positives = 38/70 (53%), Gaps = 9/70 (12%)
Query: 27 ELTKTQT-DMLLMLTDEQRCVHDK----IMDS--KGFDDGGFFFLYGYGGTGKTFIWKTS 79
EL KT T D + + +Q ++D+ ++DS +GF+ G F YG GTGKT+ + +
Sbjct: 53 ELPKTFTFDAVYDASSKQADLYDETVRPLIDSVLQGFN--GTVFAYGQTGTGKTYTMQGT 110
Query: 80 LAVVRSKGLI 89
+G+I
Sbjct: 111 WVEPELRGVI 120
>KF3C_MOUSE (O35066) Kinesin-like protein KIF3C
Length = 796
Score = 27.3 bits (59), Expect = 6.5
Identities = 23/70 (32%), Positives = 38/70 (53%), Gaps = 9/70 (12%)
Query: 27 ELTKTQT-DMLLMLTDEQRCVHDK----IMDS--KGFDDGGFFFLYGYGGTGKTFIWKTS 79
EL KT T D + + +Q ++D+ ++DS +GF+ G F YG GTGKT+ + +
Sbjct: 53 ELPKTFTFDAVYDASSKQADLYDETVRPLIDSVLQGFN--GTVFAYGQTGTGKTYTMQGT 110
Query: 80 LAVVRSKGLI 89
+G+I
Sbjct: 111 WVEPELRGVI 120
>KF3C_HUMAN (O14782) Kinesin-like protein KIF3C
Length = 793
Score = 27.3 bits (59), Expect = 6.5
Identities = 23/70 (32%), Positives = 38/70 (53%), Gaps = 9/70 (12%)
Query: 27 ELTKTQT-DMLLMLTDEQRCVHDK----IMDS--KGFDDGGFFFLYGYGGTGKTFIWKTS 79
EL KT T D + + +Q ++D+ ++DS +GF+ G F YG GTGKT+ + +
Sbjct: 53 ELPKTFTFDAVYDASSKQADLYDETVRPLIDSVLQGFN--GTVFAYGQTGTGKTYTMQGT 110
Query: 80 LAVVRSKGLI 89
+G+I
Sbjct: 111 WVEPELRGVI 120
>GIT1_YEAST (P25346) Probable metabolite transport protein GIT1
Length = 518
Score = 27.3 bits (59), Expect = 6.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Query: 74 FIWKTSLAVVRSKGLIVLNAASRVYREFYWQQTL 107
F WKT+ V KG I N + +FYW++ L
Sbjct: 237 FRWKTATTEVYEKGRIKRNIPYFLALKFYWKRLL 270
>DNA1_CHLMU (Q9PKE4) Chromosomal replication initiator protein dnaA
1
Length = 456
Score = 27.3 bits (59), Expect = 6.5
Identities = 10/38 (26%), Positives = 24/38 (62%)
Query: 63 FLYGYGGTGKTFIWKTSLAVVRSKGLIVLNAASRVYRE 100
+L+G +GKT + + ++ ++R G+ L +S+++ E
Sbjct: 144 YLFGPESSGKTHLMQAAVGILREAGVKTLYVSSQLFTE 181
>TRPF_AQUAE (O67853) N-(5'-phosphoribosyl)anthranilate isomerase (EC
5.3.1.24) (PRAI)
Length = 204
Score = 26.9 bits (58), Expect = 8.5
Identities = 11/26 (42%), Positives = 16/26 (61%)
Query: 67 YGGTGKTFIWKTSLAVVRSKGLIVLN 92
YGGTGKTF W+ + +V + L+
Sbjct: 131 YGGTGKTFNWEIAKKLVEEGHKVFLS 156
>PRIA_CHLPN (Q9Z6Y2) Primosomal protein N' (Replication factor Y)
Length = 749
Score = 26.9 bits (58), Expect = 8.5
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 2/55 (3%)
Query: 39 LTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIW--KTSLAVVRSKGLIVL 91
L EQ+ DKI S L+G G+GKT I+ TS A+ + K I+L
Sbjct: 210 LHPEQQSAIDKIFSSLKTSQFHTHLLFGITGSGKTEIYLRATSEALKQGKSTILL 264
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.140 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,638,315
Number of Sequences: 164201
Number of extensions: 535775
Number of successful extensions: 1538
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1526
Number of HSP's gapped (non-prelim): 23
length of query: 109
length of database: 59,974,054
effective HSP length: 85
effective length of query: 24
effective length of database: 46,016,969
effective search space: 1104407256
effective search space used: 1104407256
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC140721.12