BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140550.12 + phase: 0 
         (882 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

MTHK_METTH (O27564) Calcium-gated potassium channel mthK               69  7e-11
ZAP3_HUMAN (P49750) Nuclear protein ZAP3 (ZAP113)                      43  0.003
HXA3_HETFR (Q9IA21) Homeobox protein Hox-A3                            40  0.021
TNF6_MOUSE (P41047) Tumor necrosis factor ligand superfamily mem...    40  0.035
DDF1_MOUSE (Q9QWY8) 130-kDa phosphatidylinositol 4,5-biphosphate...    39  0.061
DDF1_HUMAN (Q9ULH1) 130-kDa phosphatidylinositol 4,5-biphosphate...    39  0.061
ICK_HUMAN (Q9UPZ9) Serine/threonine kinase ICK (EC 2.7.1.37) (In...    38  0.13
TOB1_RAT (Q8R5K6) Tob1 protein (Transducer of erbB-2 1)                37  0.18
ZAP3_MOUSE (Q9R0I7) Nuclear protein ZAP3                               37  0.23
AGLU_BETVU (O04931) Alpha-glucosidase precursor (EC 3.2.1.20) (M...    37  0.23
TNF6_PIG (Q9BEA8) Tumor necrosis factor ligand superfamily membe...    37  0.30
KCN3_MOUSE (P58391) Small conductance calcium-activated potassiu...    37  0.30
CYCK_MOUSE (O88874) Cyclin K                                           37  0.30
TNF6_MACNE (P63306) Tumor necrosis factor ligand superfamily mem...    36  0.51
TNF6_MACMU (P63307) Tumor necrosis factor ligand superfamily mem...    36  0.51
TNF6_MACFA (P63308) Tumor necrosis factor ligand superfamily mem...    36  0.51
SMG_DROME (Q23972) Smaug protein                                       36  0.51
GLNA_PYRAB (Q9UY99) Glutamine synthetase (EC 6.3.1.2) (Glutamate...    36  0.51
FXLG_HUMAN (Q8N461) F-box/LRR-repeat protein 16 (F-box and leuci...    36  0.51
WASL_HUMAN (O00401) Neural Wiskott-Aldrich syndrome protein (N-W...    35  0.67

>MTHK_METTH (O27564) Calcium-gated potassium channel mthK
          Length = 336

 Score = 68.6 bits (166), Expect = 7e-11
 Identities = 76/335 (22%), Positives = 149/335 (43%), Gaps = 39/335 (11%)

Query: 257 LLCATLFLIAFGGLALYAVTGGSMAEALWHSWTYVADA----GNHAETEGTGQRIVSVSI 312
           +L   L +I +G    + + G S   +L+  WT+V  A    G+++ +   G    +V++
Sbjct: 23  ILLLVLAVIIYGTAGFHFIEGESWTVSLY--WTFVTIATVGYGDYSPSTPLGMYF-TVTL 79

Query: 313 SAGGMLIFAMMLGLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQLAIANK 372
              G+  FA+ +  + + +  +      G  +V +  HV+I GWS+     L++L     
Sbjct: 80  IVLGIGTFAVAVERLLEFLINREQMKLMGLIDVAKSRHVVICGWSESTLECLRELR---- 135

Query: 373 SVGGGVIVVLAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAIIV 432
              G  + VLA  E E +   + +   +F+        G P  ++DL+K +V  ARA+IV
Sbjct: 136 ---GSEVFVLA--EDENVRKKVLRSGANFV-------HGDPTRVSDLEKANVRGARAVIV 183

Query: 433 LAADENADQSDARALRVVLSLAGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHD 492
                   +SD+  +  +L +  + E +R  ++ E    +N   +++ G +  + +    
Sbjct: 184 DL------ESDSETIHCILGIRKIDESVR--IIAEAERYENIEQLRMAGAD--QVISPFV 233

Query: 493 VIGRLMIQCALQPGLAQIWEDILGFEN----AEFYIKRWPELDDLLFKDILISFPDAIPC 548
           + GRLM +       A   +D+L  E+     E  I    +L+ +   D  I   D    
Sbjct: 234 ISGRLMSRSIDDGYEAMFVQDVLAEESTRRMVEVPIPEGSKLEGVSVLDADIH--DVTGV 291

Query: 549 GVKVAADGGKIVINPDDNYVLRDGDEVLVIAEDDD 583
            +     G +++I+P  +Y  R GD +L I + ++
Sbjct: 292 IIIGVGRGDELIIDPPRDYSFRAGDIILGIGKPEE 326


>ZAP3_HUMAN (P49750) Nuclear protein ZAP3 (ZAP113)
          Length = 1822

 Score = 43.1 bits (100), Expect = 0.003
 Identities = 34/119 (28%), Positives = 50/119 (41%), Gaps = 11/119 (9%)

Query: 17  PPLKKTKTLPSLNLRVSVTPPNPN----DNNGIGGTSTTKTDFSEQQWNYPSFLGIGST- 71
           PP      LP      S +PP+ +      +    +S++++  S  Q   PS     S  
Sbjct: 176 PPTSSQPYLPPAQPSPSQSPPSQSYLAPTPSYSSSSSSSQSYLSHSQSYLPSSQASPSRP 235

Query: 72  ----SRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTT--SSLLPQPSSSSITKQQQQ 124
               S+ +   PPPPS PP N       PL     NK+T      P+P  S++T Q+QQ
Sbjct: 236 SQGHSKSQLLAPPPPSAPPGNKTTVQQEPLESGAKNKSTEQQQAAPEPDPSTMTPQEQQ 294


>HXA3_HETFR (Q9IA21) Homeobox protein Hox-A3
          Length = 410

 Score = 40.4 bits (93), Expect = 0.021
 Identities = 31/98 (31%), Positives = 50/98 (50%), Gaps = 13/98 (13%)

Query: 26  PSLNLRVSVTPPN--PNDNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPS 83
           P+ +L+   T P+  PND N     S  +T  S+     PS   + +  ++++QPPPPP 
Sbjct: 46  PACSLQSPGTVPHHKPNDIN----ESCMRTSASQ-----PSHHPVIAEQQQQKQPPPPPP 96

Query: 84  KPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQ 121
            PP ++ P  P+  S N    +TS      S ++I+KQ
Sbjct: 97  PPPPSVSP--PQNTSSNSTQSSTSKNPTLTSQATISKQ 132


>TNF6_MOUSE (P41047) Tumor necrosis factor ligand superfamily member
           6 (FAS antigen ligand)
          Length = 279

 Score = 39.7 bits (91), Expect = 0.035
 Identities = 26/98 (26%), Positives = 46/98 (46%), Gaps = 19/98 (19%)

Query: 69  GSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHSTS 128
           G     +R+PPPPP  PPV+ +PP  +P             LP P  + + K+    +  
Sbjct: 36  GPRGPDQRRPPPPP--PPVSPLPPPSQP-------------LPLPPLTPLKKKDHNTNLW 80

Query: 129 SPIFYLLVICCIILVPYSAY----LQYKLAKLKDMKLQ 162
            P+ + +V+  ++ +    Y    LQ +LA+L++   Q
Sbjct: 81  LPVVFFMVLVALVGMGLGMYQLFHLQKELAELREFTNQ 118


>DDF1_MOUSE (Q9QWY8) 130-kDa phosphatidylinositol
           4,5-biphosphate-dependent ARF1 GTPase-activating protein
           (PIP2-dependent ARF1 GAP) (ADP-ribosylation
           factor-directed GTPase-activating protein 1) (ARF
           GTPase-activating protein 1) (Development and differen
          Length = 1147

 Score = 38.9 bits (89), Expect = 0.061
 Identities = 38/107 (35%), Positives = 42/107 (38%), Gaps = 20/107 (18%)

Query: 33  SVTPPNPNDNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPSKPPVNLIPP 92
           S  PP P  N G G T    T     Q +  S     ST  K+R PPPPP        PP
Sbjct: 803 SEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGS-----STLSKKRPPPPPPGHKRTLSDPP 857

Query: 93  HPRPLSVNDHNKTTSSLLP-----QPSSSSITKQQ-----QQHSTSS 129
            P P     H       +P      PSSSS T  +     QQ STSS
Sbjct: 858 SPLP-----HGPPNKGAIPWGNDVGPSSSSKTANKFEGLSQQASTSS 899


>DDF1_HUMAN (Q9ULH1) 130-kDa phosphatidylinositol
           4,5-biphosphate-dependent ARF1 GTPase-activating protein
           (PIP2-dependent ARF1 GAP) (ADP-ribosylation
           factor-directed GTPase-activating protein 1) (ARF
           GTPase-activating protein 1) (Development and differen
          Length = 956

 Score = 38.9 bits (89), Expect = 0.061
 Identities = 37/104 (35%), Positives = 41/104 (38%), Gaps = 20/104 (19%)

Query: 36  PPNPNDNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPSKPPVNLIPPHPR 95
           PP P  N G G T    T     Q +  S     ST  K+R PPPPP        PP P 
Sbjct: 618 PPLPPRNAGKGPTGPPSTLPLSTQTSSGS-----STLSKKRPPPPPPGHKRTLSDPPSPL 672

Query: 96  PLSVNDHNKTTSSLLP-----QPSSSSITKQQ-----QQHSTSS 129
           P     H       +P      PSSSS T  +     QQ STSS
Sbjct: 673 P-----HGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSS 711


>ICK_HUMAN (Q9UPZ9) Serine/threonine kinase ICK (EC 2.7.1.37)
           (Intestinal cell kinase) (hICK) (MAK-related kinase)
           (MRK) (Laryngeal cancer kinase 2) (LCK2)
          Length = 632

 Score = 37.7 bits (86), Expect = 0.13
 Identities = 33/126 (26%), Positives = 55/126 (43%), Gaps = 28/126 (22%)

Query: 19  LKKTKTLPSLNLRVSVT--------PPNPNDNNGIGGTST--------------TKTDFS 56
           LK ++ LP +++R  +         PPNP  ++G+ G S+              + T  S
Sbjct: 490 LKHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSS 549

Query: 57  EQQWNY-PSFLG--IGSTSRKRRQPP---PPPSKPPVNLIPPHPRPLSVNDHNKTTSSLL 110
               NY PSFL   IGS  ++    P   P P    +  + PHP     +   ++T  L+
Sbjct: 550 GLTGNYVPSFLKKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLI 609

Query: 111 PQPSSS 116
           P+P ++
Sbjct: 610 PRPPAA 615


>TOB1_RAT (Q8R5K6) Tob1 protein (Transducer of erbB-2 1)
          Length = 365

 Score = 37.4 bits (85), Expect = 0.18
 Identities = 23/65 (35%), Positives = 29/65 (44%), Gaps = 9/65 (13%)

Query: 66  LGIGSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQH 125
           LG+GS  + + QP  PPS+PP    PP P P     H +       Q       + QQQ 
Sbjct: 234 LGLGSQQQPQPQPQQPPSQPP----PPPPPPQQQQQHQQQ-----QQQQQQQQQQPQQQT 284

Query: 126 STSSP 130
           S  SP
Sbjct: 285 SALSP 289


>ZAP3_MOUSE (Q9R0I7) Nuclear protein ZAP3
          Length = 1386

 Score = 37.0 bits (84), Expect = 0.23
 Identities = 22/62 (35%), Positives = 28/62 (44%), Gaps = 2/62 (3%)

Query: 65  FLGIGSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTT--SSLLPQPSSSSITKQQ 122
           +L     S  + Q PPPPS P  N       PL     NKT       P+P  S++T Q+
Sbjct: 184 YLPPAQPSPSKPQLPPPPSIPSGNKTAIQQEPLESGAKNKTAEQKQAAPEPDPSTMTPQE 243

Query: 123 QQ 124
           QQ
Sbjct: 244 QQ 245


>AGLU_BETVU (O04931) Alpha-glucosidase precursor (EC 3.2.1.20)
           (Maltase)
          Length = 913

 Score = 37.0 bits (84), Expect = 0.23
 Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 11/63 (17%)

Query: 53  TDFSEQQWNYPSFLGIGSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQ 112
           TD + ++W  P+ +          +PPPPPS PP++ +   P+P+     N+ T+++L  
Sbjct: 97  TDANNRRWEIPNEV--------LPRPPPPPSPPPLSSLQHLPKPI---PQNQPTTTVLSH 145

Query: 113 PSS 115
           P S
Sbjct: 146 PHS 148


>TNF6_PIG (Q9BEA8) Tumor necrosis factor ligand superfamily member 6
           (FAS antigen ligand)
          Length = 282

 Score = 36.6 bits (83), Expect = 0.30
 Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 22/100 (22%)

Query: 69  GSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHSTS 128
           G   ++R  PPPPP  PP  L+P  P P             LP PS     K+++ H+  
Sbjct: 38  GRPGQRRPPPPPPPPPPPPTLLPSRPLP------------PLPPPS----LKKKRDHNAG 81

Query: 129 SPIFYLLVICCIILVPYSA------YLQYKLAKLKDMKLQ 162
             +  +  +  + LV          +LQ +L +L++   Q
Sbjct: 82  LCLLVMFFMVLVALVGLGLGMFQLFHLQKELTELRESASQ 121


>KCN3_MOUSE (P58391) Small conductance calcium-activated potassium
           channel protein 3 (SK3)
          Length = 731

 Score = 36.6 bits (83), Expect = 0.30
 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 10/58 (17%)

Query: 69  GSTSRKRRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHS 126
           G   ++++QPPPPP+ P V   PP P                PQP      +QQQQ S
Sbjct: 27  GDEQQQQQQPPPPPAPPAVPQQPPGP----------LLQPQPPQPQQQQSQQQQQQQS 74


>CYCK_MOUSE (O88874) Cyclin K
          Length = 554

 Score = 36.6 bits (83), Expect = 0.30
 Identities = 12/19 (63%), Positives = 14/19 (73%)

Query: 78  PPPPPSKPPVNLIPPHPRP 96
           PPPPP  PP + +PPHP P
Sbjct: 492 PPPPPRLPPTHAVPPHPPP 510


>TNF6_MACNE (P63306) Tumor necrosis factor ligand superfamily member
           6 (FAS antigen ligand) (CD95L protein)
          Length = 280

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 22/94 (23%)

Query: 75  RRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHSTSSPIFYL 134
           +R+PPPPP  PP  L PP P PL            LP P      K++  HST   +  +
Sbjct: 42  QRRPPPPPPPPP--LPPPPPSPLPP----------LPLPP----LKKRGNHSTGLCLLVM 85

Query: 135 LVICCIILVPYSA------YLQYKLAKLKDMKLQ 162
             +  + LV          +LQ +LA+L++   Q
Sbjct: 86  FFMVLVALVGLGLGMFQLFHLQKELAELRESTSQ 119


>TNF6_MACMU (P63307) Tumor necrosis factor ligand superfamily member
           6 (FAS antigen ligand) (CD95L protein)
          Length = 280

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 22/94 (23%)

Query: 75  RRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHSTSSPIFYL 134
           +R+PPPPP  PP  L PP P PL            LP P      K++  HST   +  +
Sbjct: 42  QRRPPPPPPPPP--LPPPPPSPLPP----------LPLPP----LKKRGNHSTGLCLLVM 85

Query: 135 LVICCIILVPYSA------YLQYKLAKLKDMKLQ 162
             +  + LV          +LQ +LA+L++   Q
Sbjct: 86  FFMVLVALVGLGLGMFQLFHLQKELAELRESTSQ 119


>TNF6_MACFA (P63308) Tumor necrosis factor ligand superfamily member
           6 (FAS antigen ligand) (CD95L protein)
          Length = 280

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 22/94 (23%)

Query: 75  RRQPPPPPSKPPVNLIPPHPRPLSVNDHNKTTSSLLPQPSSSSITKQQQQHSTSSPIFYL 134
           +R+PPPPP  PP  L PP P PL            LP P      K++  HST   +  +
Sbjct: 42  QRRPPPPPPPPP--LPPPPPSPLPP----------LPLPP----LKKRGNHSTGLCLLVM 85

Query: 135 LVICCIILVPYSA------YLQYKLAKLKDMKLQ 162
             +  + LV          +LQ +LA+L++   Q
Sbjct: 86  FFMVLVALVGLGLGMFQLFHLQKELAELRESTSQ 119


>SMG_DROME (Q23972) Smaug protein
          Length = 999

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 20/49 (40%), Positives = 27/49 (54%), Gaps = 8/49 (16%)

Query: 36  PPNPN-DNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPS 83
           PP P  D NG GG  + +  +     +YPSF+G       ++QPPPPPS
Sbjct: 838 PPLPQFDYNGYGGGPSHQPQYKSS--SYPSFMG-----NPQQQPPPPPS 879


>GLNA_PYRAB (Q9UY99) Glutamine synthetase (EC 6.3.1.2)
           (Glutamate--ammonia ligase) (GS)
          Length = 439

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 40/160 (25%), Positives = 67/160 (41%), Gaps = 26/160 (16%)

Query: 526 RWPELDDLLFKDILISFPDAIPCGVKVAADGGKIVINPDDNYVLRDGDEV---------- 575
           R P+   L+F DI     + +P G+++ A   +  I    ++   DG  V          
Sbjct: 12  RSPKFVQLIFVDI-----NGMPKGMEIPASRLQEAIEDGISF---DGSSVPGFQGIEDSD 63

Query: 576 LVIAEDDDTYAPGPLPEVRKGYFPRIRDPPKY---PEKILFCGWRRDIDDMI-MVLEAFL 631
           L+   D DTY   P   V + Y    +D   Y   P  +L    +R I+ +  M ++A++
Sbjct: 64  LIFKADPDTYVEVPWDNVARVYGYIYKDGKPYGADPRGVL----KRVIEKLAEMGIKAYI 119

Query: 632 APGSELWMFNEVPEKERERKLAAGELDVFGLENIKLVHRE 671
            P  E ++F +    E E     G  D+  L+  K + RE
Sbjct: 120 GPEPEFYLFKKNGSWELEIPDVGGYFDILTLDKAKDIKRE 159


>FXLG_HUMAN (Q8N461) F-box/LRR-repeat protein 16 (F-box and
          leucine-rich repeat protein 16) (cg14134)
          Length = 479

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 14/57 (24%)

Query: 39 PNDNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPSKPPVNLIPPHPR 95
          P   NG+G  S TK              G  +T  +  QPPPPP+ PP +L  P  R
Sbjct: 24 PGQPNGLGAASITK--------------GTPATKNRPCQPPPPPTLPPPSLAAPLSR 66


>WASL_HUMAN (O00401) Neural Wiskott-Aldrich syndrome protein
           (N-WASP)
          Length = 505

 Score = 35.4 bits (80), Expect = 0.67
 Identities = 31/100 (31%), Positives = 41/100 (41%), Gaps = 10/100 (10%)

Query: 32  VSVTPPNPNDNNGIGGTSTTKTDFSEQQWNYPSFLGIGSTSRKRRQPPPPPSKPPVNLIP 91
           V V PP PN        +   +  S      PS LG+G  +     PPPPP  P     P
Sbjct: 332 VEVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGP-----P 386

Query: 92  PHPRPLSVNDHNKTT-----SSLLPQPSSSSITKQQQQHS 126
           P P   S  DH   T     ++LL Q    +  K+ +Q+S
Sbjct: 387 PPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNS 426


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.319    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,043,007
Number of Sequences: 164201
Number of extensions: 4666517
Number of successful extensions: 19633
Number of sequences better than 10.0: 104
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 84
Number of HSP's that attempted gapping in prelim test: 18715
Number of HSP's gapped (non-prelim): 543
length of query: 882
length of database: 59,974,054
effective HSP length: 119
effective length of query: 763
effective length of database: 40,434,135
effective search space: 30851245005
effective search space used: 30851245005
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 70 (31.6 bits)