BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140549.12 - phase: 0
(504 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RGS7_CAEEL (Q8WQC0) Regulator of G-protein signaling rgs-7 (Regu... 36 0.27
RIM_CAEEL (Q22366) Rab-3 interacting molecule unc-10 (Rim) (Unco... 35 0.35
DMP1_HUMAN (Q13316) Dentin matrix acidic phosphoprotein 1 precur... 35 0.59
POLR_KYMVJ (P36304) RNA replicase polyprotein (EC 2.7.7.48) 33 1.3
COX1_DINSE (O79548) Cytochrome c oxidase polypeptide I (EC 1.9.3.1) 33 1.3
LEU1_SYNY3 (P48576) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 33 1.7
GAG_FIVT2 (P31821) Gag polyprotein [Contains: Core protein p15; ... 33 1.7
GAG_EIAVY (P03351) Gag polyprotein [Contains: Core protein p15; ... 33 1.7
YS89_CAEEL (Q09624) Hypothetical protein ZK945.9 in chromosome II 33 2.2
Y060_MYCPN (P75042) Putative glycosyl transferase MG060 homolog ... 33 2.2
HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding p... 33 2.2
LEU1_ANASP (P48575) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 32 2.9
ZAN_HUMAN (Q9Y493) Zonadhesin precursor 32 3.8
S24A_HUMAN (O95486) Protein transport protein Sec24A (SEC24-rela... 32 3.8
LIP_STAAW (Q8NYC2) Lipase precursor (EC 3.1.1.3) (Glycerol ester... 32 3.8
LEU1_MICAE (P94907) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 32 3.8
GLO3_YEAST (P38682) ADP-ribosylation factor GTPase-activating pr... 32 3.8
CNB3_HUMAN (Q9NQW8) Cyclic-nucleotide-gated cation channel beta ... 32 3.8
SLU7_YEAST (Q02775) Pre-mRNA splicing factor SLU7 32 5.0
POLG_FMDV1 (P03306) Genome polyprotein [Contains: Nonstructural ... 32 5.0
>RGS7_CAEEL (Q8WQC0) Regulator of G-protein signaling rgs-7
(Regulator of G-protein signaling c2) (Protein C2-RGS)
Length = 819
Score = 35.8 bits (81), Expect = 0.27
Identities = 53/213 (24%), Positives = 82/213 (37%), Gaps = 65/213 (30%)
Query: 308 AINSQLMACNQNGNSIIDHNKDV---------TANKRNA-----INRIDDNQVLHGDWLV 353
A+ Q++ Q+ +++ +NK V AN+R+ I R DD+ D V
Sbjct: 65 AVRRQILMNQQHEPNVLGNNKKVYQTFGSTPQLANERHKEMYSQIRRGDDSSFRSRDRFV 124
Query: 354 VTRRKK--------------TPIQHSLNASKSVTHK-----------------TNRFQAL 382
RR + +PI+ S +S S H+ T FQ L
Sbjct: 125 PPRRPRMGYGSSDLSAIEEMSPIRSSTYSSSSEAHRLSSLRAQTEWKPQLTSTTTSFQPL 184
Query: 383 STMTHHDK-----SAPINNKFPSWSKSQEIPRANNRSTETKRRRQEDFYEPIAKNLSTND 437
S M HHDK AP++ S +SQ + R N K RRQ +L ++
Sbjct: 185 SPMNHHDKENGPFKAPMSPVCSSTPRSQRMYRKN-----PKYRRQ------FGSSLQLSE 233
Query: 438 SRHITILEPVLSLKTKDTSTSKIDITQPHSTEH 470
SR LE S +++ T + + + EH
Sbjct: 234 SR----LEESTSQESERAVTPESWMEHNNENEH 262
>RIM_CAEEL (Q22366) Rab-3 interacting molecule unc-10 (Rim)
(Uncoordinated protein 10)
Length = 1563
Score = 35.4 bits (80), Expect = 0.35
Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 5/73 (6%)
Query: 264 RNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPRQNPTQTCPDAINSQLMACNQNGNSI 323
+N M+ P N+H G NN N + +Q +Q + IN NQN N
Sbjct: 215 QNQMQNPHQNQNHVQNNHQ-GANNHQQNNRRAMQQQPMSQNQANQINQM----NQNQNQQ 269
Query: 324 IDHNKDVTANKRN 336
HN+++T N+RN
Sbjct: 270 QSHNQNMTQNQRN 282
>DMP1_HUMAN (Q13316) Dentin matrix acidic phosphoprotein 1 precursor
(Dentin matrix protein-1) (DMP-1)
Length = 513
Score = 34.7 bits (78), Expect = 0.59
Identities = 29/131 (22%), Positives = 59/131 (44%), Gaps = 7/131 (5%)
Query: 369 SKSVTHKTNRFQALSTMTHHDKSAPIN-NKFPSWSKSQEIPRANNRSTETKRRRQE---D 424
S+ + H + + S ++ D + ++ N KS +N+R T + R++ D
Sbjct: 257 SQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGD 316
Query: 425 FYEPIAKNLSTNDSRHITILEPVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNNHEPVTT 484
E +NLS +S+++ + S +++ + S + + E V+ +N +P T+
Sbjct: 317 SQEDSKENLSQEESQNV---DGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTS 373
Query: 485 YTEDDTIPDPS 495
Y ED D S
Sbjct: 374 YVEDQEDSDSS 384
>POLR_KYMVJ (P36304) RNA replicase polyprotein (EC 2.7.7.48)
Length = 1874
Score = 33.5 bits (75), Expect = 1.3
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 7/52 (13%)
Query: 269 KPSTFDPRSPNHHTAGGNNPP-------SNPEPSQPRQNPTQTCPDAINSQL 313
KP + +P +PN ++ G+NPP + P P++P + T P + N L
Sbjct: 665 KPESSNPNNPNPSSSAGSNPPPKSSSSDNPPAPNKPTPTSSSTTPPSPNLPL 716
>COX1_DINSE (O79548) Cytochrome c oxidase polypeptide I (EC 1.9.3.1)
Length = 533
Score = 33.5 bits (75), Expect = 1.3
Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Query: 157 VTHWTPEFA--SPNAKVDRTVVWVRFPGLNLVYYDESFL 193
+THW P F + N + +T WV F G+N+ ++ + FL
Sbjct: 393 LTHWFPLFTGYTLNQTMTKTQFWVMFVGVNMTFFPQHFL 431
>LEU1_SYNY3 (P48576) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 533
Score = 33.1 bits (74), Expect = 1.7
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
Query: 95 LLGKSLGYNTMKDRLQKIWKLQGGFDIMDNDNGFFMVKFDQAADKEKVIT 144
+LGK G N RL+++ GFD+ D + ++F + ADK K IT
Sbjct: 344 VLGKLSGRNAFGTRLKEL-----GFDLSDTELNNAFIRFKEVADKRKEIT 388
>GAG_FIVT2 (P31821) Gag polyprotein [Contains: Core protein p15;
Major core protein p24; Nucleic acid binding protein
p10]
Length = 449
Score = 33.1 bits (74), Expect = 1.7
Identities = 19/52 (36%), Positives = 22/52 (41%), Gaps = 4/52 (7%)
Query: 244 VQYEGLHLICTNCGCYGHLGRNCMEKPSTFDPRSPNHHTA----GGNNPPSN 291
VQ +G L+C NC GHL R C E + P H A GG N
Sbjct: 368 VQTKGPRLVCFNCKKPGHLARQCKEAKRCNNCGKPGHLAANCWQGGRKTSGN 419
>GAG_EIAVY (P03351) Gag polyprotein [Contains: Core protein p15;
Major core protein p26 (Capsid protein p26); Core
protein p11; Core protein p9]
Length = 486
Score = 33.1 bits (74), Expect = 1.7
Identities = 18/60 (30%), Positives = 23/60 (38%)
Query: 253 CTNCGCYGHLGRNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPRQNPTQTCPDAINSQ 312
C NCG GHL C F + P H + + P N + + QT P SQ
Sbjct: 383 CYNCGKPGHLSSQCRAPKVCFKCKQPGHFSKQCRSVPKNGKQGAQGRPQKQTFPIQQKSQ 442
>YS89_CAEEL (Q09624) Hypothetical protein ZK945.9 in chromosome II
Length = 3178
Score = 32.7 bits (73), Expect = 2.2
Identities = 30/102 (29%), Positives = 40/102 (38%), Gaps = 7/102 (6%)
Query: 402 SKSQEIPRANNRSTETKRRRQEDFYEPIAKNLSTNDSRHITILEPVLSLKTKDTSTSKID 461
S S + +T T EP ++T+ S PV S T +S+S
Sbjct: 418 STSSTVTTTEPTTTLTTSTASTSTTEPSTSTVTTSPST-----SPVTSTVTSSSSSSTT- 471
Query: 462 ITQPHSTEHV-TNPSQNNHEPVTTYTEDDTIPDPSSSMPQDT 502
+T P STE T+PS T + T P SSS P T
Sbjct: 472 VTTPTSTESTSTSPSSTVTTSTTAPSTSTTGPSSSSSTPSST 513
>Y060_MYCPN (P75042) Putative glycosyl transferase MG060 homolog (EC
2.-.-.-) (D09_orf299)
Length = 299
Score = 32.7 bits (73), Expect = 2.2
Identities = 30/148 (20%), Positives = 60/148 (40%), Gaps = 13/148 (8%)
Query: 333 NKRNAINRIDDNQVLHGDWLVVTRRKKTPIQHSLNASKSVTHKTNRFQALSTMTHHDKSA 392
N + IN + +N++ +GDW+ V T ++LN ++ K + + T K
Sbjct: 70 NWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKCWKKI 129
Query: 393 PIN-NKFPSWSKSQEIPRANNRSTETKRRRQEDFYEPIAKNLSTNDSRHITILEPVLSLK 451
+ + +P+W K+ + ++Q F P+ K L N + L+ +S +
Sbjct: 130 KLKIHTYPTWWKNM----------TRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQ 179
Query: 452 TKDTSTSKIDITQPHSTEHVTNPSQNNH 479
+ S + + + HV N H
Sbjct: 180 DALYTASSLKLAK--KVRHVNQSGGNYH 205
>HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding
protein)
Length = 271
Score = 32.7 bits (73), Expect = 2.2
Identities = 17/46 (36%), Positives = 19/46 (40%)
Query: 253 CTNCGCYGHLGRNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPR 298
C NCG GH R C E S D RS G S P++ R
Sbjct: 18 CRNCGKEGHYARECPEADSKGDERSTTCFRCGEEGHMSRECPNEAR 63
>LEU1_ANASP (P48575) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 531
Score = 32.3 bits (72), Expect = 2.9
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Query: 95 LLGKSLGYNTMKDRLQKIWKLQGGFDIMDNDNGFFMVKFDQAADKEKVIT 144
+LGK G N + RL+++ GF++ + + VKF + ADK+K I+
Sbjct: 344 VLGKHSGRNAFRTRLKEL-----GFELSETELNKAFVKFKEVADKKKEIS 388
>ZAN_HUMAN (Q9Y493) Zonadhesin precursor
Length = 2812
Score = 32.0 bits (71), Expect = 3.8
Identities = 23/77 (29%), Positives = 32/77 (40%), Gaps = 2/77 (2%)
Query: 428 PIAKNLSTNDSRHITILE-PVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNNHEPVTTYT 486
P++ ST S + E P +S K S K +T T P+ +P T T
Sbjct: 544 PVSPVSSTGPSETTGLTENPTISTKKPTVSIEKPSVTTEKPTVPKEKPTIPTEKP-TIST 602
Query: 487 EDDTIPDPSSSMPQDTP 503
E TIP +MP + P
Sbjct: 603 EKPTIPSEKPNMPSEKP 619
>S24A_HUMAN (O95486) Protein transport protein Sec24A (SEC24-related
protein A) (Fragment)
Length = 1078
Score = 32.0 bits (71), Expect = 3.8
Identities = 18/61 (29%), Positives = 27/61 (43%), Gaps = 3/61 (4%)
Query: 270 PSTFDPRSPNHHTAGGNNPP---SNPEPSQPRQNPTQTCPDAINSQLMACNQNGNSIIDH 326
P+TF P +P+ G PP P S R P A+N + + N N S++ H
Sbjct: 186 PTTFQPGAPHGPPPAGGPPPVRALTPLTSSYRDVPQPLFNSAVNQEGITSNTNNGSMVVH 245
Query: 327 N 327
+
Sbjct: 246 S 246
>LIP_STAAW (Q8NYC2) Lipase precursor (EC 3.1.1.3) (Glycerol ester
hydrolase)
Length = 690
Score = 32.0 bits (71), Expect = 3.8
Identities = 25/112 (22%), Positives = 46/112 (40%), Gaps = 2/112 (1%)
Query: 318 QNGNSIIDHNKDVTANKRNAINRIDDNQVLHGDWLVVTRR-KKTPIQHSLNASKSVTHKT 376
QN N + + + +K++ N +N D + T ++ Q + + S V
Sbjct: 110 QNDNQV-KQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQ 168
Query: 377 NRFQALSTMTHHDKSAPINNKFPSWSKSQEIPRANNRSTETKRRRQEDFYEP 428
A + +HDK+AP + PS K+ +T K Q+D ++P
Sbjct: 169 PSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQP 220
>LEU1_MICAE (P94907) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 533
Score = 32.0 bits (71), Expect = 3.8
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Query: 95 LLGKSLGYNTMKDRLQKIWKLQGGFDIMDNDNGFFMVKFDQAADKEKVIT 144
+LGK G N + RLQ++ GF++ + + ++F + AD++K IT
Sbjct: 345 VLGKLSGRNAFRSRLQEL-----GFELSETELNNAFIQFKEMADRKKEIT 389
>GLO3_YEAST (P38682) ADP-ribosylation factor GTPase-activating
protein GLO3
Length = 493
Score = 32.0 bits (71), Expect = 3.8
Identities = 39/209 (18%), Positives = 72/209 (33%), Gaps = 20/209 (9%)
Query: 286 NNPPSNPEPSQPRQNPTQTCPDAINSQLMACNQNGNSIIDHNKDVTANKRNAINRIDDNQ 345
++ P + + R + D S + N +S ++ N A K N
Sbjct: 151 SDSPLDTDSDASRSTSKENSVDDFFSNWQKPSSNSSSKLNVNTGSLAPKNNTTGSTPKTT 210
Query: 346 VLHGDWLVVTRRKKTPIQHSLNASKSVTHKTNRFQALSTMTHHDKS--APINNKFPSWSK 403
V ++T +K P+ +S + K ++R T DKS + ++F ++
Sbjct: 211 VTKTRSSILTASRKKPVLNSQDKKKHSILSSSRKPTRLTAKKVDKSQAEDLFDQFKKEAQ 270
Query: 404 SQEIPRANNRSTETKRRRQEDFYEPIAKNLSTNDSRHITILEPVLSLKTKDTSTSKIDIT 463
++ N S+ TK RQ D+ N N++ I + T
Sbjct: 271 QEKEDEFTNSSSSTK-IRQNDYDSQFMNNSKGNNNNSIDDIN-----------------T 312
Query: 464 QPHSTEHVTNPSQNNHEPVTTYTEDDTIP 492
QP N + N+ + +D P
Sbjct: 313 QPDEFNDFLNDTSNSFDTTRKEQQDTLTP 341
>CNB3_HUMAN (Q9NQW8) Cyclic-nucleotide-gated cation channel beta 3
(CNG channel beta 3) (Cyclic nucleotide gated channel
beta 3) (Cone photoreceptor cGMP-gated channel beta
subunit) (Cyclic nucleotide-gated cation channel
modulatory subunit)
Length = 809
Score = 32.0 bits (71), Expect = 3.8
Identities = 28/112 (25%), Positives = 46/112 (41%), Gaps = 14/112 (12%)
Query: 398 FPSWSKSQEI-PRANNRSTETKRRRQEDFYEPIAKNLSTNDSRHITILEP----VLSLKT 452
F S +K ++ P N E RR E+ P +N S+ T E SLKT
Sbjct: 2 FKSLTKVNKVKPIGENNENEQSSRRNEEGSHP------SNQSQQTTAQEENKGEEKSLKT 55
Query: 453 KDTSTSKIDITQPHSTEHVTNPSQNNHEPVTTYTEDDTIPDPSSSMPQDTPM 504
K T + +PH+ +N+ +TT + +P+ ++P+ M
Sbjct: 56 KSTPVTS---EEPHTNIQDKLSKKNSSGDLTTNPDPQNAAEPTGTVPEQKEM 104
>SLU7_YEAST (Q02775) Pre-mRNA splicing factor SLU7
Length = 382
Score = 31.6 bits (70), Expect = 5.0
Identities = 13/34 (38%), Positives = 18/34 (52%)
Query: 253 CTNCGCYGHLGRNCMEKPSTFDPRSPNHHTAGGN 286
C NCG GH ++CMEKP P+ ++ N
Sbjct: 122 CRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNN 155
>POLG_FMDV1 (P03306) Genome polyprotein [Contains: Nonstructural
protein P20A; Coat protein VP4; Coat protein VP3; Coat
protein VP2; Coat protein VP1; Core protein P52;
Genome-linked protein VPG1; Genome-linked protein VPG2;
Genome-linked protein VPG3; Pi
Length = 2333
Score = 31.6 bits (70), Expect = 5.0
Identities = 34/155 (21%), Positives = 61/155 (38%), Gaps = 17/155 (10%)
Query: 343 DNQVLHGDWLVVTRRKKTPIQHSLNASKSVTHKTNRFQALST--MTHHDKSAPINNKFPS 400
D + L+GDW ++K S A+ S N ++ M + S +
Sbjct: 184 DQEPLNGDWKTQVQKKLKGAGQSSPATGSQNQSGNTGSIINNYYMQQYQNSMSTQLGDNT 243
Query: 401 WSKSQEIPRANNRSTETKRRRQEDFYEPIAKN---------LSTNDSRHITILEP-VLSL 450
S + ST T + D++ +A + L+ + T+LE +L+
Sbjct: 244 ISGGSNEGSTDTTSTHTTNTQNNDWFSKLASSAFTGLFGALLADKKTEETTLLEDRILTT 303
Query: 451 K---TKDTSTSKIDITQPHSTE--HVTNPSQNNHE 480
+ T T+ S + +T +STE HV P+ + E
Sbjct: 304 RNGHTTSTTQSSVGVTYGYSTEEDHVAGPNTSGLE 338
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.132 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,096,583
Number of Sequences: 164201
Number of extensions: 2919659
Number of successful extensions: 9435
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 47
Number of HSP's that attempted gapping in prelim test: 9356
Number of HSP's gapped (non-prelim): 100
length of query: 504
length of database: 59,974,054
effective HSP length: 115
effective length of query: 389
effective length of database: 41,090,939
effective search space: 15984375271
effective search space used: 15984375271
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 68 (30.8 bits)
Medicago: description of AC140549.12