BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140068.2 + phase: 0
(501 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PUSH_ARATH (O22928) Putative pseudouridylate synthase (EC 4.2.1.... 490 e-138
PUS1_YEAST (Q12211) Pseudouridylate synthase 1 (EC 4.2.1.70) (Ps... 107 7e-23
TRUA_MOUSE (Q9WU56) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 100 1e-20
TRUA_HUMAN (Q9Y606) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 99 3e-20
PUS2_YEAST (P53167) Pseudouridylate synthase 2 (EC 4.2.1.70) (Ps... 68 6e-11
TRUA_WIGBR (Q8D2P5) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 66 2e-10
TRUA_METTH (O26928) Probable tRNA pseudouridine synthase A (EC 4... 66 2e-10
TRUA_METAC (Q8TU65) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 66 2e-10
PUS3_YEAST (P31115) Pseudouridylate synthase 3 (EC 4.2.1.70) (Ps... 66 2e-10
TRUA_BORBU (P70830) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 65 3e-10
TRUA_METMA (Q8PWT1) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 63 2e-09
PUS3_MOUSE (Q9JI38) Pseudouridylate synthase 3 (EC 4.2.1.70) (Ps... 63 2e-09
TRA2_CLOTE (Q890R5) tRNA pseudouridine synthase A 2 (EC 4.2.1.70... 62 3e-09
TRUA_METKA (Q8TWZ3) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 62 3e-09
PUS3_HUMAN (Q9BZE2) Pseudouridylate synthase 3 (EC 4.2.1.70) (Ps... 61 6e-09
TRUA_CHLTR (O84469) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 58 5e-08
TRUA_ARCFU (O28544) Probable tRNA pseudouridine synthase A (EC 4... 58 5e-08
TRUA_THETN (Q8R7Y7) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 58 7e-08
TRUA_ANASP (Q8YPK5) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 57 1e-07
TRUA_RHOBA (Q7TU07) tRNA pseudouridine synthase A (EC 4.2.1.70) ... 56 2e-07
>PUSH_ARATH (O22928) Putative pseudouridylate synthase (EC 4.2.1.70)
(Pseudouridine synthase)
Length = 510
Score = 490 bits (1262), Expect = e-138
Identities = 251/452 (55%), Positives = 320/452 (70%), Gaps = 39/452 (8%)
Query: 51 WQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKI 110
W+ +RKKKVV+RI Y GT+YRGLQ+QRD S+ T+E ELE AI+KAGGIR+SN GDL KI
Sbjct: 57 WESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKI 116
Query: 111 GWARSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQ 170
GWARSSRTDKGVHSLAT ++ KMEIPE +W +DP G LA ++ +LP +I+V S+LP+
Sbjct: 117 GWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSN 176
Query: 171 RSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSR 230
R FDPR+EC LRKYSYLLP D++GI++ ++DEIDYH+++FN+ILKEFEG +PFHNYT R
Sbjct: 177 RRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQR 236
Query: 231 SRYRR-----------HIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQ 279
SRYRR PR P K E + E +EEE +VD
Sbjct: 237 SRYRRKSEQKIKQRNGRPPREPKSIKASESEFREENHIEIGEEEEEKEVD---------- 286
Query: 280 NQKSSGTSDSCEPVIETGNKGNLHDQSSSSVV-RAKWLYEPDEADRLNASHFRRVFRCSS 338
G SD E V+ D +S V RAKWLYEPDE D+++ +HFR+VFRC S
Sbjct: 287 -----GESD--EHVVTP-------DSDNSQVYSRAKWLYEPDETDKISGAHFRKVFRCRS 332
Query: 339 GKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPL 398
GKLE S+G ++EI I GESFMLHQIRKM+ TAVAVKR+LLPRDII LSL+KF+RI+LPL
Sbjct: 333 GKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPL 392
Query: 399 APSEVLLLRGNSFSMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVSKFLDPSR 458
APSEVL+LRGNSF +R P RP M+ ESEE+ K +++FYR+VM+PQVS FLD +
Sbjct: 393 APSEVLILRGNSFEVRRLP---ERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEK 449
Query: 459 VPWVEWIEKFDAHTSIPNDQLDEVRKARNLWK 490
PW EW++ D + + +++L++VRK WK
Sbjct: 450 SPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481
>PUS1_YEAST (Q12211) Pseudouridylate synthase 1 (EC 4.2.1.70)
(Pseudouridine synthase 1)
Length = 544
Score = 107 bits (267), Expect = 7e-23
Identities = 101/358 (28%), Positives = 158/358 (43%), Gaps = 26/358 (7%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+KV + + Y GT Y G+Q T+E L A +AG I + N DL+K G+ R+
Sbjct: 74 KRKVAVMVGYCGTGYHGMQYNPPN---PTIESALFKAFVEAGAISKDNSNDLKKNGFMRA 130
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGVH+ +++ KM I EDP + +N LP I+V I ++FD
Sbjct: 131 ARTDKGVHAGGNLISLKMII------EDP---DIKQKINEKLPEGIRVWDIERVNKAFDC 181
Query: 176 RKECVLRKYSYLLPA-DIIGIQSHS-SNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSRY 233
RK C R Y YLLP +IG + S +I+ +E +L E F +
Sbjct: 182 RKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELPGVLDEDLESKEFW-----EEF 236
Query: 234 RRHIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCEPV 293
++ + S + IL+ +++ D E +E +++ S +
Sbjct: 237 KKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAK 296
Query: 294 IETGNKGNLHDQSSSSVVRAKWLYEPDEADRLNASHFRRVFRCSSGKLEKSMGHSYIEIY 353
L + + K EP +A F + + S + +I I
Sbjct: 297 FRASTSQYLGAHNFHNFTLGKDFKEP------SAIRFMKDIKVSDPFVIGDAQTEWISIK 350
Query: 354 IEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRGNSF 411
I G+SFMLHQIRKMV+ A + R P + I+ + + +I +P AP+ LLL F
Sbjct: 351 IHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQ-QKINIPKAPALGLLLEAPVF 407
>TRUA_MOUSE (Q9WU56) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 393
Score = 99.8 bits (247), Expect = 1e-20
Identities = 57/190 (30%), Positives = 101/190 (53%), Gaps = 16/190 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ +AG I E++ D+ K+ + R
Sbjct: 49 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 108
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + + +NS+LP I+++ + F+
Sbjct: 109 ARTDKGVSAAGQVVSLKVWLIDD----------ILDKINSHLPSHIRILGLKRVTGGFNS 158
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ +C R Y Y+LP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 159 KNKCDARTYCYMLPTFAFAHKDRDVQDE-SYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 217
Query: 231 SRYRRHIPRR 240
R RR
Sbjct: 218 KGPREPSARR 227
Score = 42.4 bits (98), Expect = 0.003
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S + ++ +P AP L+
Sbjct: 242 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLV 300
Query: 406 L 406
L
Sbjct: 301 L 301
>TRUA_HUMAN (Q9Y606) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 399
Score = 99.0 bits (245), Expect = 3e-20
Identities = 62/206 (30%), Positives = 105/206 (50%), Gaps = 23/206 (11%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ ++G I E++ D+ K+ + R
Sbjct: 55 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 114
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + +NS+LP I+++ + F+
Sbjct: 115 ARTDKGVSAAGQVVSLKVWLIDD----------ILEKINSHLPSHIRILGLKRVTGGFNS 164
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ C R Y YLLP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 165 KNRCDARTYCYLLPTFAFAHKDRDVQDE-TYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 223
Query: 231 SRYRRHIPRRPSHSKCGEILSAYNSE 256
P+ P S C IL Y E
Sbjct: 224 KG-----PQDP--SACRYILEMYCEE 242
Score = 41.6 bits (96), Expect = 0.005
Identities = 22/61 (36%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S ++ +P AP L+
Sbjct: 248 GLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT-EKVDVPKAPGLGLV 306
Query: 406 L 406
L
Sbjct: 307 L 307
>PUS2_YEAST (P53167) Pseudouridylate synthase 2 (EC 4.2.1.70)
(Pseudouridine synthase 2)
Length = 370
Score = 67.8 bits (164), Expect = 6e-11
Identities = 40/133 (30%), Positives = 68/133 (51%), Gaps = 13/133 (9%)
Query: 61 MRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSRTDK 120
M + Y G+ Y G+Q T+E E+ + +F G I E N +K + ++RTDK
Sbjct: 1 MLLGYCGSGYYGMQYNPPHK---TIEGEILTKLFDVGAISEENSLAPKKNSFMAAARTDK 57
Query: 121 GVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRKECV 180
GVH++ +++ K+ + E++ +N+ LP +I+V I P + F+ R C
Sbjct: 58 GVHAMLNLLSLKITLREDT----------VAKLNAALPPEIRVWGIQPVNKKFNARSACD 107
Query: 181 LRKYSYLLPADII 193
R Y YL+P I+
Sbjct: 108 SRWYQYLIPEFIL 120
Score = 51.6 bits (122), Expect = 5e-06
Identities = 29/63 (46%), Positives = 39/63 (61%)
Query: 349 YIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRG 408
+I + I G+SFMLHQIR+MVA AV R LP +I+ + R +P AP++ LLL G
Sbjct: 246 WICVRIHGQSFMLHQIRRMVALAVLAARCQLPPNIVRNYFNAGPRKYIPRAPAQGLLLEG 305
Query: 409 NSF 411
F
Sbjct: 306 PVF 308
>TRUA_WIGBR (Q8D2P5) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 264
Score = 66.2 bits (160), Expect = 2e-10
Identities = 54/186 (29%), Positives = 93/186 (49%), Gaps = 27/186 (14%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
KK+ I+Y G NY G Q Q++ + ++++ +E A+FK G EKI +
Sbjct: 2 KKLAAGISYQGNNYCGWQSQKN---IISIQEYVELALFKFSG---------EKIKIYCAG 49
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTD VH+L +V F +I + S+ + G VNSYLP DI ++ ++ +F R
Sbjct: 50 RTDAKVHALLQVVHFYTDI-KRSYRQWILG------VNSYLPYDISIMWVIKVHNNFHAR 102
Query: 177 KECVLRKYSYLLPADI--IGI-QSHSSNDEIDYHMSEFNDILKEFEGGHPFHNY-----T 228
+ +KY YL+ +I GI + + N I ++++ ++ G H F ++
Sbjct: 103 YSAIAKKYLYLIHNNISRSGIFRKYCFNVFISLNINDMKKAAQKLIGKHDFSSFKGSECQ 162
Query: 229 SRSRYR 234
S++ YR
Sbjct: 163 SKTTYR 168
>TRUA_METTH (O26928) Probable tRNA pseudouridine synthase A (EC
4.2.1.70) (Pseudouridylate synthase I) (Pseudouridine
synthase I) (Uracil hydrolyase)
Length = 275
Score = 66.2 bits (160), Expect = 2e-10
Identities = 56/175 (32%), Positives = 80/175 (45%), Gaps = 32/175 (18%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
+K+ +++AY GTNY G Q Q D + TVE +L A+ AG I D ++ + +
Sbjct: 2 RKIALKVAYIGTNYHGFQRQPD---VPTVEGKLLEALEGAGIIE-----DPKRARFQIAG 53
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTD+GVH+L V+F E ED N +N LP DI+V++ F R
Sbjct: 54 RTDRGVHALGNFVSFFTE-------ED----IHVNQINDLLPRDIRVLAWASVMYPFKVR 102
Query: 177 KECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRS 231
+ R Y Y+L H Y M+E F G H F N++ RS
Sbjct: 103 YP-LERHYRYIL---------HREESMDTYSMAE---AAAHFRGTHDFSNFSRRS 144
Score = 32.0 bits (71), Expect = 3.8
Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
Query: 350 IEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRGN 409
I++Y GESF+ +RKMV + V L D + L R+ L P E L+L
Sbjct: 166 IDVY--GESFLWQMVRKMVRALLMVSEGELAPDDMAGLLDTDRRVFLEPMPPENLILMDL 223
Query: 410 SFSMRTK 416
+ ++ K
Sbjct: 224 KYGVKIK 230
>TRUA_METAC (Q8TU65) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 269
Score = 66.2 bits (160), Expect = 2e-10
Identities = 51/171 (29%), Positives = 74/171 (42%), Gaps = 30/171 (17%)
Query: 58 KVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSR 117
+V +++AY GT + G Q+Q + + TVEKEL A+ I D G R
Sbjct: 2 RVALKLAYIGTEFHGSQIQPN---VETVEKELFKALRNLSIIESPKSADYTCAG-----R 53
Query: 118 TDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRK 177
TD GVH+L +VAF E P A+ +NS LP I + FD R+
Sbjct: 54 TDAGVHALGQVVAFDTEKP---------NLAIPRVINSELPPAIWAWAHAEVPYYFDARR 104
Query: 178 ECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYT 228
V R Y Y++ + DY +S+ + K G H F N++
Sbjct: 105 SAVSRHYHYVMSGE-------------DYDISKMREASKLLLGTHDFENFS 142
>PUS3_YEAST (P31115) Pseudouridylate synthase 3 (EC 4.2.1.70)
(Pseudouridine synthase 3) (Depressed growth-rate
protein DEG1)
Length = 442
Score = 65.9 bits (159), Expect = 2e-10
Identities = 45/164 (27%), Positives = 78/164 (47%), Gaps = 17/164 (10%)
Query: 46 PQSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLG 105
P+ + + + + +R AY G NY GL +Q++ L TVE + A+ K + L
Sbjct: 81 PKKFDFSKHNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQ 140
Query: 106 DLEKIGWARSSRTDKGVHSLATMVAFKMEI---------PENSWNEDPFGFALANYVNSY 156
D + ++R RTDKGV ++ +++ ++ P N E P+ + +N
Sbjct: 141 DYK---FSRCGRTDKGVSAMNQVISLEVRSNLTDEEQRDPTNDSREIPY----VHVLNQL 193
Query: 157 LPCDIKVISI-LPAQRSFDPRKECVLRKYSYLLPADIIGIQSHS 199
LP DI++ ++ L +FD R CV R Y Y+ + I+ S
Sbjct: 194 LPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGKNLNIEKMS 237
>TRUA_BORBU (P70830) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 246
Score = 65.5 bits (158), Expect = 3e-10
Identities = 57/190 (30%), Positives = 90/190 (47%), Gaps = 34/190 (17%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
KK++ IAY G+ Y G Q+Q + TV+ E+E A+ K + +K+ S
Sbjct: 2 KKILAEIAYDGSIYHGFQIQPTK---PTVQGEIEKALMKI---------NKKKVKIHSSG 49
Query: 117 RTDKGVHSLATMVAF--KMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
RTDKGVH+ ++ F K+ I N+ + L N IK++ + + SF
Sbjct: 50 RTDKGVHAKKQIITFDIKINIQLNNLKKALNAILLKN--------SIKILKLRYVKNSFH 101
Query: 175 PRKECVLRKYSY-LLPADII----GIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYT- 228
PR RKYSY +L +D G Q+H N ++ +S N + K G H F ++
Sbjct: 102 PRFSAQKRKYSYCILNSDNYYPWEGYQAHYVNKKLS--ISNLNQMAKTLIGKHDFTTFSC 159
Query: 229 ----SRSRYR 234
S+S++R
Sbjct: 160 IKDKSKSKFR 169
>TRUA_METMA (Q8PWT1) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 269
Score = 63.2 bits (152), Expect = 2e-09
Identities = 51/172 (29%), Positives = 79/172 (45%), Gaps = 32/172 (18%)
Query: 58 KVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEK-IGWARSS 116
+V +++AY GT + G Q+Q S+ TVEKEL FKA +R + + K + +
Sbjct: 2 RVALKLAYIGTEFHGSQIQP---SVETVEKEL----FKA--LRNLRIIESPKSANYICAG 52
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTD GVH+L ++AF + P A+ +NS LP I V + FD R
Sbjct: 53 RTDAGVHALGQVIAFDTDRPN---------LAIPRIINSGLPPTIWVWAHAEVPYDFDAR 103
Query: 177 KECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYT 228
+ V R Y Y++ + Y +S+ + K G H F N++
Sbjct: 104 RHAVSRHYRYVISGE-------------GYDISKMREASKLLLGTHDFENFS 142
>PUS3_MOUSE (Q9JI38) Pseudouridylate synthase 3 (EC 4.2.1.70)
(Pseudouridine synthase 3)
Length = 481
Score = 62.8 bits (151), Expect = 2e-09
Identities = 45/192 (23%), Positives = 84/192 (43%), Gaps = 32/192 (16%)
Query: 47 QSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGD 106
+++ + ++ V ++IAY G Y+G Q +N+ +T+E++L A+ K + +
Sbjct: 54 RAFDFSAHGRRHVALKIAYLGWGYQGFASQ--ENTSNTIEEKLFEALTKTRLVESRQTSN 111
Query: 107 LEKIGWARSSRTDKGVHSLATMVAFKM--EIPENSWNEDPF----------GFALANYVN 154
+ G RTDKGV + +++ + + P + +ED + +N
Sbjct: 112 YHRCG-----RTDKGVSAFGQVISLDLRSQFPTSRDSEDSNLKHEADDLAKEIRYTHILN 166
Query: 155 SYLPCDIKVISILPAQRSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDI 214
LP DI+V++ P + SF R C+ R Y Y P D ++ N
Sbjct: 167 RVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFP-------------RADLDIATMNYA 213
Query: 215 LKEFEGGHPFHN 226
+++ G H F N
Sbjct: 214 AQKYVGTHDFRN 225
>TRA2_CLOTE (Q890R5) tRNA pseudouridine synthase A 2 (EC 4.2.1.70)
(Pseudouridylate synthase I 2) (Pseudouridine synthase I
2) (Uracil hydrolyase 2)
Length = 248
Score = 62.4 bits (150), Expect = 3e-09
Identities = 44/132 (33%), Positives = 66/132 (49%), Gaps = 21/132 (15%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
+ V +++ Y GTNY G Q Q+D+N + T++ +E A I E+ ++E IG SS
Sbjct: 3 RNVRLKVEYDGTNYSGWQKQKDKN-IKTIQSSIEKA------IEEATKEEVELIG---SS 52
Query: 117 RTDKGVHSLATMVAFK--MEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
RTD GVH+LA FK IP G + +N +LP DI ++ F
Sbjct: 53 RTDAGVHALAYTANFKTCSTIP---------GEKFKHALNRFLPEDIVILESEEVPMEFH 103
Query: 175 PRKECVLRKYSY 186
R +C+ + Y Y
Sbjct: 104 SRFDCIGKTYVY 115
>TRUA_METKA (Q8TWZ3) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 256
Score = 62.0 bits (149), Expect = 3e-09
Identities = 44/131 (33%), Positives = 68/131 (51%), Gaps = 24/131 (18%)
Query: 58 KVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSR 117
+V +R+AY G+ Y G Q Q D + T+E L A+ + G LE +G+A SR
Sbjct: 2 RVALRVAYDGSRYHGFQYQPD---VPTIEGALRKALSELG---------LELVGYA--SR 47
Query: 118 TDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRK 177
TD G H+ +V + + PE + DP +N+ LP DI+VI+ FDPR+
Sbjct: 48 TDAGAHARYQVVVVEGD-PELA-QPDP--------INARLPKDIRVIAKTEVHEEFDPRR 97
Query: 178 ECVLRKYSYLL 188
+ + ++Y Y L
Sbjct: 98 DALRKEYRYFL 108
>PUS3_HUMAN (Q9BZE2) Pseudouridylate synthase 3 (EC 4.2.1.70)
(Pseudouridine synthase 3) (FKSG32 protein)
Length = 481
Score = 61.2 bits (147), Expect = 6e-09
Identities = 49/194 (25%), Positives = 86/194 (44%), Gaps = 32/194 (16%)
Query: 45 TPQSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNL 104
T +++ + ++ V +RIAY G Y+G Q + N+ T+E++L A+ K +
Sbjct: 51 TKRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNN--TIEEKLFEALTKTRLVESRQT 108
Query: 105 GDLEKIGWARSSRTDKGVHSLATMVAFKM--EIPENSWNEDPFGFALAN----------Y 152
+ + G RTDKGV + +++ + + P +ED AN
Sbjct: 109 SNYHRCG-----RTDKGVSAFGQVISLDLRSQFPRGRDSEDFNVKEEANAAAEEIRYTHI 163
Query: 153 VNSYLPCDIKVISILPAQRSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFN 212
+N LP DI++++ P + SF R C+ R Y Y P + I + +DY
Sbjct: 164 LNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVT------MDY------ 211
Query: 213 DILKEFEGGHPFHN 226
+++ G H F N
Sbjct: 212 -AAQKYVGTHDFRN 224
>TRUA_CHLTR (O84469) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 267
Score = 58.2 bits (139), Expect = 5e-08
Identities = 48/179 (26%), Positives = 82/179 (44%), Gaps = 19/179 (10%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
KK+V++IAY GT+Y G Q Q N+LS +++ LE+ + K G R S + S
Sbjct: 3 KKIVLQIAYQGTSYSGWQYQ--PNALS-IQEVLETILKKIAGFRISVIS---------SG 50
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTD GVH+ + F P++ DP + +N+ LP DI + + F R
Sbjct: 51 RTDAGVHAQGQIAHF--HCPDHPHFTDP--RQIQKMLNALLPHDIVIRDAVMTDEDFHSR 106
Query: 177 KECVLRKYSY---LLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSR 232
+ ++Y Y LLP + + + +++ + + G H F ++ + R
Sbjct: 107 FSAIAKEYRYTLSLLPKPLPHHRLFCFSPRYKLNIARMQEAAQYLVGTHDFASFANLGR 165
>TRUA_ARCFU (O28544) Probable tRNA pseudouridine synthase A (EC
4.2.1.70) (Pseudouridylate synthase I) (Pseudouridine
synthase I) (Uracil hydrolyase)
Length = 265
Score = 58.2 bits (139), Expect = 5e-08
Identities = 49/173 (28%), Positives = 74/173 (42%), Gaps = 37/173 (21%)
Query: 58 KVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSR 117
K +IAY G N+ G Q Q DQ TVE E+ +A+ + G +E + R
Sbjct: 3 KFAFKIAYFGDNFHGSQFQPDQR---TVEGEVINALRRLG---------VENPRLRSAGR 50
Query: 118 TDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRK 177
TD GVH+ +++F ++ED +N+ LP DI + FDPR+
Sbjct: 51 TDAGVHAYGQVISF--------YSEDKI---FPRMLNAELPEDITAWAWAKVSEDFDPRR 99
Query: 178 ECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSR 230
R Y+Y++ DY +S +KE G H F N+T +
Sbjct: 100 -AKSRVYTYVMYGS-------------DYDISAMRKAVKELIGVHDFSNFTKK 138
>TRUA_THETN (Q8R7Y7) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 240
Score = 57.8 bits (138), Expect = 7e-08
Identities = 44/128 (34%), Positives = 60/128 (46%), Gaps = 23/128 (17%)
Query: 63 IAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSRTDKGV 122
I Y GTNY G Q Q++ TV++ LE AI K G EK+ SSRTD GV
Sbjct: 4 IEYDGTNYHGWQYQKNA---ITVQEVLEKAIEKVTG---------EKVNLIGSSRTDTGV 51
Query: 123 HSLATMVAF--KMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRKECV 180
H+L + F K +IP L +N+ LP D+ V+ +SF R
Sbjct: 52 HALYQVANFKTKTKIPPEK---------LPYALNAVLPDDVVVVKAKEVDKSFHARYSAR 102
Query: 181 LRKYSYLL 188
++Y YL+
Sbjct: 103 SKRYRYLI 110
>TRUA_ANASP (Q8YPK5) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 282
Score = 57.0 bits (136), Expect = 1e-07
Identities = 42/141 (29%), Positives = 66/141 (46%), Gaps = 23/141 (16%)
Query: 50 SWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEK 109
S QP + ++V + + Y GT++ G Q Q+ TV++E+E+AI K G + G
Sbjct: 4 SHQPKQTQRVALVVQYLGTHFHGWQRQKQHR---TVQEEIETAIAKILGHHVTLHG---- 56
Query: 110 IGWARSSRTDKGVHSLATMVAFKME--IPENSWNEDPFGFALANYVNSYLPCDIKVISIL 167
+ RTD GVH+ A + F IP + W A+ +NSYLP DI + +
Sbjct: 57 -----AGRTDSGVHAAAQVAHFDATGLIPAHKW---------ASVINSYLPKDILIKASA 102
Query: 168 PAQRSFDPRKECVLRKYSYLL 188
+ R R+Y Y +
Sbjct: 103 AVGDRWHARFSAAYRRYRYTI 123
>TRUA_RHOBA (Q7TU07) tRNA pseudouridine synthase A (EC 4.2.1.70)
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase)
Length = 276
Score = 55.8 bits (133), Expect = 2e-07
Identities = 41/134 (30%), Positives = 62/134 (45%), Gaps = 18/134 (13%)
Query: 55 RKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWAR 114
R + + +AY GT Y G Q+Q +Q S ++ ELE I G LG
Sbjct: 6 RPRTFHLTVAYDGTEYCGWQVQPEQRS---IQSELERVIRPLAGRPVRVLG--------- 53
Query: 115 SSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
S RTD GVH++ + + + W D AL +NS LP DI+V ++ + F
Sbjct: 54 SGRTDAGVHAIGQVARCVLPV----WKADTV--ALQRAINSKLPNDIRVKAVRETRERFH 107
Query: 175 PRKECVLRKYSYLL 188
P + ++Y YL+
Sbjct: 108 PIADATGKQYQYLV 121
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,358,583
Number of Sequences: 164201
Number of extensions: 2592922
Number of successful extensions: 6971
Number of sequences better than 10.0: 158
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 137
Number of HSP's that attempted gapping in prelim test: 6762
Number of HSP's gapped (non-prelim): 205
length of query: 501
length of database: 59,974,054
effective HSP length: 114
effective length of query: 387
effective length of database: 41,255,140
effective search space: 15965739180
effective search space used: 15965739180
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC140068.2