BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138452.7 + phase: 0 /pseudo
(677 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SA1_MOUSE (Q9D3E6) Cohesin subunit SA-1 (Stromal antigen 1) (SCC... 37 0.22
SA1_HUMAN (Q8WVM7) Cohesin subunit SA-1 (Stromal antigen 1) (SCC... 37 0.22
Z335_HUMAN (Q9H4Z2) Zinc finger protein 335 36 0.29
RGP1_MOUSE (P46061) Ran GTPase-activating protein 1 35 0.50
DNAK_LACPL (Q88VM0) Chaperone protein dnaK (Heat shock protein 7... 35 0.84
SIS2_CANTR (Q12600) SIS2 protein (Halotolerance protein HAL3) 34 1.1
GA2B_BOVIN (Q28034) Glucosidase II beta subunit precursor (Prote... 34 1.1
SIS2_YEAST (P36024) SIS2 protein (Halotolerance protein HAL3) 34 1.4
SPBP_RAT (P08723) Prostatic spermine-binding protein precursor (... 33 1.9
SET_RAT (Q63945) SET protein (Phosphatase 2A inhibitor I2PP2A) (... 33 1.9
SET_MOUSE (Q9EQU5) SET protein (Phosphatase 2A inhibitor I2PP2A)... 33 1.9
SET_HUMAN (Q01105) SET protein (Phosphatase 2A inhibitor I2PP2A)... 33 1.9
TOM1_NEUCR (Q9P4Z1) E3 ubiquitin protein ligase TOM1-like protei... 33 2.5
SA1_XENLA (Q9DGN1) Cohesin subunit SA-1 (XSA-1) (Stromal antigen... 33 2.5
GIM4_HUMAN (Q9NUV9) GTPase, IMAP family member 4 (Immunity-assoc... 33 2.5
YP58_YEAST (Q99299) Hypothetical protein YPL158C 33 3.2
NPM_XENLA (P07222) Nucleophosmin (NPM) (Nucleolar phosphoprotein... 33 3.2
GYRB_CLOAB (P94604) DNA gyrase subunit B (EC 5.99.1.3) 33 3.2
TOP1_USTMA (P41511) DNA topoisomerase I (EC 5.99.1.2) 32 4.2
NUCL_MESAU (P08199) Nucleolin (Protein C23) 32 4.2
>SA1_MOUSE (Q9D3E6) Cohesin subunit SA-1 (Stromal antigen 1) (SCC3
homolog 1)
Length = 1258
Score = 36.6 bits (83), Expect = 0.22
Identities = 52/244 (21%), Positives = 91/244 (36%), Gaps = 40/244 (16%)
Query: 108 LEKANLIVVDADGDKDGAADADLE-----IISGGRGKRKSAGEIAHKNMLKEDSLR*NKA 162
+E L+ + G ++ ++ D E + S R +AGE HK + + +A
Sbjct: 391 VEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEA 450
Query: 163 QSMVCGRRSTGKMLVRLLLSFFYNNAISFNVA--------RSDEYLK----MFELVAKHG 210
+ GR S L+R+L+ FF + + + A S E LK M EL+ +
Sbjct: 451 LAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEP 510
Query: 211 IGFKPLSYDEIRNKYLNYFYGEIGKDLEAHRAVWEKCGCIIMT----DGWTDRRRRTILN 266
+ + D + + I + EAH V G ++T D R + +
Sbjct: 511 VQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEH 570
Query: 267 FLVHSP-------------------KGTFFLKSIDPSDITKTADKIFKMIDDVVEEIGEE 307
F++ P F L+ + K D + K I VVE+ E
Sbjct: 571 FIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVES 630
Query: 308 NVVQ 311
+V++
Sbjct: 631 DVLE 634
>SA1_HUMAN (Q8WVM7) Cohesin subunit SA-1 (Stromal antigen 1) (SCC3
homolog 1)
Length = 1258
Score = 36.6 bits (83), Expect = 0.22
Identities = 52/244 (21%), Positives = 91/244 (36%), Gaps = 40/244 (16%)
Query: 108 LEKANLIVVDADGDKDGAADADLE-----IISGGRGKRKSAGEIAHKNMLKEDSLR*NKA 162
+E L+ + G ++ ++ D E + S R +AGE HK + + +A
Sbjct: 391 VEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEA 450
Query: 163 QSMVCGRRSTGKMLVRLLLSFFYNNAISFNVA--------RSDEYLK----MFELVAKHG 210
+ GR S L+R+L+ FF + + + A S E LK M EL+ +
Sbjct: 451 LAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEP 510
Query: 211 IGFKPLSYDEIRNKYLNYFYGEIGKDLEAHRAVWEKCGCIIMT----DGWTDRRRRTILN 266
+ + D + + I + EAH V G ++T D R + +
Sbjct: 511 VQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEH 570
Query: 267 FLVHSP-------------------KGTFFLKSIDPSDITKTADKIFKMIDDVVEEIGEE 307
F++ P F L+ + K D + K I VVE+ E
Sbjct: 571 FIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVES 630
Query: 308 NVVQ 311
+V++
Sbjct: 631 DVLE 634
>Z335_HUMAN (Q9H4Z2) Zinc finger protein 335
Length = 1342
Score = 36.2 bits (82), Expect = 0.29
Identities = 26/107 (24%), Positives = 37/107 (34%), Gaps = 12/107 (11%)
Query: 2 ASTSNVNETSSVASSAPVASSSAAARVRPKNAKGNKTDPAWEYAISTEPGSRRVQCKFCQ 61
A T V ++ S ASS P S + V P + S++ CK C
Sbjct: 978 AKTHCVGDSQSSASSPPATSKALGLAVPPSPPS------------AATAASKKFSCKICA 1025
Query: 62 LAFTGSAYRVKHHLAGTSRDVAVCPSVPLDVKKLMQDKVNKLQKKLL 108
AF G A H A CP P ++ + + + Q L
Sbjct: 1026 EAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSL 1072
>RGP1_MOUSE (P46061) Ran GTPase-activating protein 1
Length = 589
Score = 35.4 bits (80), Expect = 0.50
Identities = 14/25 (56%), Positives = 19/25 (76%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQE 663
+EEG+ DD+E E+ EDE DDD +E
Sbjct: 368 EEEGEEDDEEEEDEEDEEDDDEEEE 392
>DNAK_LACPL (Q88VM0) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 622
Score = 34.7 bits (78), Expect = 0.84
Identities = 32/118 (27%), Positives = 55/118 (46%), Gaps = 8/118 (6%)
Query: 67 SAYRVKHHLAGTSRDVAVCPSV------PLDVKK-LMQDKVNKLQKKLLEKANLIVVDAD 119
+A + K L+G S P + PL ++ L + K N+L L+EK + V +A
Sbjct: 238 AAEKAKKDLSGVSEAQISLPFISAGASGPLHLETTLTRAKFNELTADLVEKTRIPVENAL 297
Query: 120 GDKD-GAADADLEIISGGRGKRKSAGEIAHKNMLKEDSLR*NKAQSMVCGRRSTGKML 176
D D A+D D+ I++GG + + E K KE + N +++ G G ++
Sbjct: 298 KDADLSASDLDVVILNGGSTRIPAVQEAVEKWTGKESNHSINPDEAVALGAAVQGGVI 355
>SIS2_CANTR (Q12600) SIS2 protein (Halotolerance protein HAL3)
Length = 531
Score = 34.3 bits (77), Expect = 1.1
Identities = 12/28 (42%), Positives = 22/28 (77%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFPP 666
D++ D+DDD+ ++++D+ DDD +E PP
Sbjct: 488 DDDDDDDDDDDDDDDDDDDDDDDEEDPP 515
Score = 31.6 bits (70), Expect = 7.1
Identities = 11/34 (32%), Positives = 22/34 (64%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFPPAMGIND 672
D++ D+DDD+ ++++D+ DDD ++ P D
Sbjct: 489 DDDDDDDDDDDDDDDDDDDDDDEEDPPQQQSTTD 522
>GA2B_BOVIN (Q28034) Glucosidase II beta subunit precursor (Protein
kinase C substrate, 60.1 kDa protein, heavy chain)
(PKCSH) (80K-H protein) (Vacuolar system associated
protein-60) (VASAP-60)
Length = 533
Score = 34.3 bits (77), Expect = 1.1
Identities = 14/30 (46%), Positives = 21/30 (69%)
Query: 636 PSIDEEGDNDDDEVEENEDEYDDDPYQEFP 665
P+ +E+ D +D+E EE+EDE D+D E P
Sbjct: 311 PTEEEDEDEEDEETEEDEDEEDEDSQGEQP 340
>SIS2_YEAST (P36024) SIS2 protein (Halotolerance protein HAL3)
Length = 562
Score = 33.9 bits (76), Expect = 1.4
Identities = 16/39 (41%), Positives = 25/39 (64%)
Query: 625 VNGIGVLLKGLPSIDEEGDNDDDEVEENEDEYDDDPYQE 663
VN I + L G P +EE D+D+DE E++++E D + E
Sbjct: 481 VNKIVMKLGGYPKNNEEEDDDEDEEEDDDEEEDTEDKNE 519
Score = 31.2 bits (69), Expect = 9.3
Identities = 12/34 (35%), Positives = 23/34 (67%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFPPAMGIND 672
D++ D+DDD+ ++++D+ DDD ++ GI D
Sbjct: 526 DDDDDDDDDDDDDDDDDDDDDEDEDEAETPGIID 559
>SPBP_RAT (P08723) Prostatic spermine-binding protein precursor
(SBP)
Length = 279
Score = 33.5 bits (75), Expect = 1.9
Identities = 28/94 (29%), Positives = 47/94 (49%), Gaps = 18/94 (19%)
Query: 591 LKHQLNGGNLMDLDIR-----NCSGLL*EYLV*HVAHQGVNGI---GVLLKGL----PSI 638
L N G ++ +R N SG +YLV VNG+ G+ L G+ +I
Sbjct: 99 LSFTTNKGRVVTFGVRRGLSFNESGGSDKYLV------TVNGLYAPGLCLNGMGFKWKNI 152
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFPPAMGIND 672
++ D++DD+ E+++DE+DDD ++ ND
Sbjct: 153 HDDFDDNDDDKEDDDDEHDDDNEEDHGDKDNDND 186
>SET_RAT (Q63945) SET protein (Phosphatase 2A inhibitor I2PP2A)
(I-2PP2A) (Template activating factor I) (TAF-I) (Liver
regeneration related protein LRRGR00002) (Ab1-115)
Length = 289
Score = 33.5 bits (75), Expect = 1.9
Identities = 14/31 (45%), Positives = 21/31 (67%)
Query: 633 KGLPSIDEEGDNDDDEVEENEDEYDDDPYQE 663
+GL IDEEGD D+ E +++EDE ++ E
Sbjct: 255 EGLEDIDEEGDEDEGEEDDDEDEGEEGEEDE 285
>SET_MOUSE (Q9EQU5) SET protein (Phosphatase 2A inhibitor I2PP2A)
(I-2PP2A) (Template activating factor I) (TAF-I)
Length = 289
Score = 33.5 bits (75), Expect = 1.9
Identities = 14/31 (45%), Positives = 21/31 (67%)
Query: 633 KGLPSIDEEGDNDDDEVEENEDEYDDDPYQE 663
+GL IDEEGD D+ E +++EDE ++ E
Sbjct: 255 EGLEDIDEEGDEDEGEEDDDEDEGEEGEEDE 285
>SET_HUMAN (Q01105) SET protein (Phosphatase 2A inhibitor I2PP2A)
(I-2PP2A) (Template activating factor I) (TAF-I) (HLA-DR
associated protein II) (PHAPII) (Inhibitor of granzyme
A-activated DNase) (IGAAD)
Length = 290
Score = 33.5 bits (75), Expect = 1.9
Identities = 14/31 (45%), Positives = 21/31 (67%)
Query: 633 KGLPSIDEEGDNDDDEVEENEDEYDDDPYQE 663
+GL IDEEGD D+ E +E++DE ++ E
Sbjct: 256 EGLEDIDEEGDEDEGEEDEDDDEGEEGEEDE 286
>TOM1_NEUCR (Q9P4Z1) E3 ubiquitin protein ligase TOM1-like protein (EC
6.3.2.-)
Length = 4065
Score = 33.1 bits (74), Expect = 2.5
Identities = 14/23 (60%), Positives = 18/23 (77%), Gaps = 1/23 (4%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPY 661
DE+G+ DDDE +E ED+ DDD Y
Sbjct: 2600 DEDGEEDDDE-DEGEDDMDDDMY 2621
>SA1_XENLA (Q9DGN1) Cohesin subunit SA-1 (XSA-1) (Stromal antigen 1
homolog) (SCC3 homolog 1)
Length = 1265
Score = 33.1 bits (74), Expect = 2.5
Identities = 36/163 (22%), Positives = 66/163 (40%), Gaps = 17/163 (10%)
Query: 108 LEKANLIVVDADGDKDGAADADLE-----IISGGRGKRKSAGEIAHKNMLKEDSLR*NKA 162
+E L+ + G ++ ++ D E + S R +AGE HK + + +A
Sbjct: 398 VEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEA 457
Query: 163 QSMVCGRRSTGKMLVRLLLSFFYNNAISFNVA--------RSDEYLK----MFELVAKHG 210
+ GR S LV++L+ FF + + + A S E LK M EL+ +
Sbjct: 458 LAKRRGRSSPNGNLVKMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLVEEP 517
Query: 211 IGFKPLSYDEIRNKYLNYFYGEIGKDLEAHRAVWEKCGCIIMT 253
+ + + + + + I + EAH V G ++T
Sbjct: 518 MQGEEVMSERQESALVELMVCTIRQAAEAHPPVGRGTGKRVLT 560
>GIM4_HUMAN (Q9NUV9) GTPase, IMAP family member 4
(Immunity-associated protein 4) (Immunity-associated
nucleotide 1 protein) (hIAN1) (MSTP062)
Length = 329
Score = 33.1 bits (74), Expect = 2.5
Identities = 38/147 (25%), Positives = 57/147 (37%), Gaps = 18/147 (12%)
Query: 421 KSNEWKTSKLAKSKDGKIVQNVVLSKVFWKDVLNCLRGAFPLVKVLCMVDWEEKAAMGYI 480
+S+ WK ++L I V + K+++ C+ P L +V +G
Sbjct: 72 RSSSWKETELVVVDTPGIFDTEVPNAETSKEIIRCILLTSPGPHALLLV-----VPLGRY 126
Query: 481 YEEMDRAKEKIRTNFSGQARSYN-PIWKIIDDRWDRQLHRPLHAAGLFLNPMLRYAPNFT 539
EE +A EKI F +ARS+ I+ DD D LH LR AP
Sbjct: 127 TEEEHKATEKILKMFGERARSFMILIFTRKDDLGDTNLH-----------DYLREAPEDI 175
Query: 540 VD-DEIVNGMYACLRRMVADAEKRKKK 565
D +I Y L AE+ ++
Sbjct: 176 QDLMDIFGDRYCALNNKATGAEQEAQR 202
>YP58_YEAST (Q99299) Hypothetical protein YPL158C
Length = 758
Score = 32.7 bits (73), Expect = 3.2
Identities = 25/94 (26%), Positives = 45/94 (47%), Gaps = 6/94 (6%)
Query: 570 LRISTQRLENLVMTLLPMHLKLKHQLNGGNLMDLDIRNCSGLL*EYLV*HVAHQGVNGIG 629
LR+ R++N ++ M+ + N DL LL EY + Q +G+
Sbjct: 594 LRVQIARIQNYKSNVVYMNKSA--ETNSTIYEDL----VDSLLTEYEADGSSSQIFDGVT 647
Query: 630 VLLKGLPSIDEEGDNDDDEVEENEDEYDDDPYQE 663
V DE+ ++D++E EEN+DE D++ ++
Sbjct: 648 VRADEEEEEDEDDEDDEEEEEENDDEEDEEDEED 681
Score = 31.2 bits (69), Expect = 9.3
Identities = 12/25 (48%), Positives = 20/25 (80%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQE 663
+EE +NDD+E EE+E++ +DD +E
Sbjct: 665 EEEEENDDEEDEEDEEDDEDDEEEE 689
>NPM_XENLA (P07222) Nucleophosmin (NPM) (Nucleolar phosphoprotein
B23) (Numatrin) (Nucleolar protein NO38)
Length = 299
Score = 32.7 bits (73), Expect = 3.2
Identities = 12/27 (44%), Positives = 20/27 (73%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFP 665
+E+ D DDD+ E+++DE DD+ +E P
Sbjct: 163 EEDSDEDDDDDEDDDDEDDDEEEEETP 189
>GYRB_CLOAB (P94604) DNA gyrase subunit B (EC 5.99.1.3)
Length = 637
Score = 32.7 bits (73), Expect = 3.2
Identities = 39/148 (26%), Positives = 63/148 (42%), Gaps = 18/148 (12%)
Query: 214 KPLSYDEIRNKYLNYFYGEIGKDLEAHRAVWEKCGCIIMTDGWTDRR--RRTILNFLVHS 271
K L+ +EI+ F G IGKD + + + K IIMTD D R IL F
Sbjct: 462 KILNSEEIK-AMATAFGGGIGKDFDVSKLRYHKI--IIMTDADVDGAHIRTLILTFFYRY 518
Query: 272 -----PKGTFFLKSIDPSDITKTADKIF----KMIDDVVEEIGEENVVQVVTDNAANYKA 322
+G F+ +TKT + + K ++DV++++G ++ + YK
Sbjct: 519 MTELISEGHVFIAQPPLYKVTKTRKEYYAYSDKELEDVLQDVGGKDK----NTDIQRYKG 574
Query: 323 AGELLMEKRPHLYWTPCAAHCIDLMLED 350
GE+ E+ P I + +ED
Sbjct: 575 LGEMNPEQLWETTMNPEQRTLIKVNIED 602
>TOP1_USTMA (P41511) DNA topoisomerase I (EC 5.99.1.2)
Length = 1019
Score = 32.3 bits (72), Expect = 4.2
Identities = 11/22 (50%), Positives = 19/22 (86%)
Query: 639 DEEGDNDDDEVEENEDEYDDDP 660
D+EGD+DD+E ++++D+ DD P
Sbjct: 117 DDEGDDDDEEDDDDDDDDDDKP 138
>NUCL_MESAU (P08199) Nucleolin (Protein C23)
Length = 713
Score = 32.3 bits (72), Expect = 4.2
Identities = 13/30 (43%), Positives = 21/30 (69%)
Query: 639 DEEGDNDDDEVEENEDEYDDDPYQEFPPAM 668
DE+ D+DDDE + +EDE D++ + PP +
Sbjct: 144 DEDEDDDDDEDDSDEDEEDEEEDEFEPPVV 173
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,451,239
Number of Sequences: 164201
Number of extensions: 3461777
Number of successful extensions: 16724
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 15936
Number of HSP's gapped (non-prelim): 407
length of query: 677
length of database: 59,974,054
effective HSP length: 117
effective length of query: 560
effective length of database: 40,762,537
effective search space: 22827020720
effective search space used: 22827020720
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 69 (31.2 bits)
Medicago: description of AC138452.7