BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137994.3 - phase: 0
(155 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SEGN_HUMAN (O76038) Secretagogin 33 0.25
SVC1_RAT (Q9WTW7) Solute carrier family 23, member 1 (Sodium-dep... 31 0.96
GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin) 31 0.96
GDA4_WHEAT (P04724) Alpha/beta-gliadin A-IV precursor (Prolamin) 31 0.96
SVC1_HUMAN (Q9UHI7) Solute carrier family 23, member 1 (Sodium-d... 31 1.3
SVC1_MOUSE (Q9Z2J0) Solute carrier family 23, member 1 (Sodium-d... 30 1.6
SYK3_HAEIN (P43826) Putative lysyl-tRNA synthetase (EC 6.1.1.6) ... 30 2.1
SYL_STAEP (Q8CNU8) Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine-... 30 2.8
PDM2_DROME (P31369) POU domain protein 2 (didymous protein) (Pdm... 30 2.8
CPN_DROME (Q02910) Calphotin 30 2.8
CASB_PIG (P39037) Beta casein precursor 30 2.8
YHT1_YEAST (P38835) Hypothetical 95.1 kDa protein in ACT5-YCK1 i... 29 3.6
MUC2_HUMAN (Q02817) Mucin 2 precursor (Intestinal mucin 2) 29 4.8
GATB_GLOVI (Q7NI42) Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransfe... 29 4.8
YKF4_YEAST (P35732) Hypothetical 84.0 kDa protein in NUP120-CSE4... 28 8.1
PTR1_SCHPO (O13834) E3 ubiquitin protein ligase ptr1 (EC 6.3.2.-... 28 8.1
NQO1_PARDE (P29913) NADH-quinone oxidoreductase chain 1 (EC 1.6.... 28 8.1
>SEGN_HUMAN (O76038) Secretagogin
Length = 276
Score = 33.1 bits (74), Expect = 0.25
Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 3/77 (3%)
Query: 70 QWWRDFTAQIRPHLEEKEWTKIFLKTLDQFYYDNMVASAPNNFAEMVTMSMHLEEGVREG 129
Q W+ F A + ++EEKE FL L + D+ V A N ++ M ++ ++G
Sbjct: 19 QVWQRFDADEKGYIEEKELDAFFLHMLMKLGTDDTVMKA--NLHKVKQQFMTTQDASKDG 76
Query: 130 RL-VKENVPTDRSEDED 145
R+ +KE SEDE+
Sbjct: 77 RIRMKELAGMFLSEDEN 93
>SVC1_RAT (Q9WTW7) Solute carrier family 23, member 1
(Sodium-dependent vitamin C transporter 1)
(Na(+)/L-ascorbic acid transporter 1)
Length = 604
Score = 31.2 bits (69), Expect = 0.96
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Query: 7 WSTPLSSGEVFRPVVSEVQ-APFVQQTTPIPQTGLPFPQAAMTYSSPLT 54
WS PL++ ++ P + EVQ A V + L P A ++Y PLT
Sbjct: 146 WSMPLNTSHIWHPRIREVQGAIMVSSVVEVVIGLLGLPGALLSYIGPLT 194
>GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin)
Length = 307
Score = 31.2 bits (69), Expect = 0.96
Identities = 15/61 (24%), Positives = 28/61 (45%)
Query: 27 PFVQQTTPIPQTGLPFPQAAMTYSSPLTQNDKESFKTYFKAYAQWWRDFTAQIRPHLEEK 86
PF Q P PQ LP+PQ + Y P ++ + Y+Q + + Q + +++
Sbjct: 80 PFPQPQLPYPQPQLPYPQPQLPYPQPQPFRPQQPYPQSQPQYSQPQQPISQQQQQQQQQQ 139
Query: 87 E 87
+
Sbjct: 140 Q 140
>GDA4_WHEAT (P04724) Alpha/beta-gliadin A-IV precursor (Prolamin)
Length = 297
Score = 31.2 bits (69), Expect = 0.96
Identities = 18/74 (24%), Positives = 36/74 (48%), Gaps = 1/74 (1%)
Query: 25 QAPFVQQTTPIPQTGLPFPQAAMTYSSPLTQNDKESFKTYFKAYAQWWRDFTAQIRPHLE 84
Q P++Q P PQ LP+PQ + Y P ++S+ Y+Q + + Q + +
Sbjct: 72 QQPYMQ-LQPFPQPQLPYPQPQLPYPQPQPFRPQQSYPQPQPQYSQPQQPISQQQQQQQQ 130
Query: 85 EKEWTKIFLKTLDQ 98
+++ + L+ + Q
Sbjct: 131 QQQQQQQILQQILQ 144
>SVC1_HUMAN (Q9UHI7) Solute carrier family 23, member 1
(Sodium-dependent vitamin C transporter 1) (hSVCT1)
(Na(+)/L-ascorbic acid transporter 1) (Yolk sac
permease-like molecule 3)
Length = 598
Score = 30.8 bits (68), Expect = 1.3
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 7 WSTPLSSGEVFRPVVSEVQ-APFVQQTTPIPQTGLPFPQAAMTYSSPLT 54
WS PL++ ++ P + EVQ A V + L P A + Y PLT
Sbjct: 139 WSLPLNTSHIWHPRIREVQGAIMVSSVVEVVIGLLGLPGALLNYIGPLT 187
>SVC1_MOUSE (Q9Z2J0) Solute carrier family 23, member 1
(Sodium-dependent vitamin C transporter 1)
(Na(+)/L-ascorbic acid transporter 1) (Yolk sac
permease-like molecule 3)
Length = 605
Score = 30.4 bits (67), Expect = 1.6
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Query: 7 WSTPLSSGEVFRPVVSEVQAPFVQQTTPIPQTGL-PFPQAAMTYSSPLT 54
WS PL++ ++ P + EVQ + + GL P A ++Y PLT
Sbjct: 146 WSMPLNTSHIWHPRIREVQGAIMVSSMVEVVIGLMGLPGALLSYIGPLT 194
>SYK3_HAEIN (P43826) Putative lysyl-tRNA synthetase (EC 6.1.1.6)
(Lysine--tRNA ligase) (LysRS) (GX)
Length = 323
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/45 (37%), Positives = 22/45 (48%)
Query: 22 SEVQAPFVQQTTPIPQTGLPFPQAAMTYSSPLTQNDKESFKTYFK 66
SEV P + + PI P QAA+ SP Q E F+ Y+K
Sbjct: 195 SEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYK 239
>SYL_STAEP (Q8CNU8) Leucyl-tRNA synthetase (EC 6.1.1.4)
(Leucine--tRNA ligase) (LeuRS)
Length = 814
Score = 29.6 bits (65), Expect = 2.8
Identities = 25/95 (26%), Positives = 47/95 (49%), Gaps = 13/95 (13%)
Query: 59 ESFKT--YFKAYAQWWRDFTAQIRPHLEEKEWTKIFLKTLDQFYYDNMVASAP-NNFAEM 115
E +KT +K Y + + + I PH+ E+ W D+ ++N + P F E
Sbjct: 696 ECYKTNEVYKPYIEGFVKMLSPIAPHIGEELW--------DRLGHENTITYQPWPTFDES 747
Query: 116 VTMSMHLEEGVR-EGRL-VKENVPTDRSEDEDQEV 148
+ + +E V+ G++ K N+P D S++E Q++
Sbjct: 748 LLVDDEVEIVVQVNGKVRAKINIPKDLSKEEMQDL 782
>PDM2_DROME (P31369) POU domain protein 2 (didymous protein) (Pdm-2)
(dPOU-28) (dOct2) (miti-mere)
Length = 498
Score = 29.6 bits (65), Expect = 2.8
Identities = 21/64 (32%), Positives = 29/64 (44%), Gaps = 1/64 (1%)
Query: 8 STPLSSGEVFRPVVSEVQAPFVQQTTPIPQTGLPFPQA-AMTYSSPLTQNDKESFKTYFK 66
S +SS + S Q P +QQ+TP P +GL A A SP D E + + K
Sbjct: 240 SLGMSSAVMTPNTPSMQQQPQLQQSTPKPTSGLTVASAMAKLEQSPEETTDLEELEQFAK 299
Query: 67 AYAQ 70
+ Q
Sbjct: 300 TFKQ 303
>CPN_DROME (Q02910) Calphotin
Length = 865
Score = 29.6 bits (65), Expect = 2.8
Identities = 12/36 (33%), Positives = 21/36 (58%)
Query: 10 PLSSGEVFRPVVSEVQAPFVQQTTPIPQTGLPFPQA 45
P+ + V + V V+ P + +T IP+T + FP+A
Sbjct: 613 PVEAPVVIQEAVDAVEVPVTETSTSIPETTVEFPEA 648
>CASB_PIG (P39037) Beta casein precursor
Length = 232
Score = 29.6 bits (65), Expect = 2.8
Identities = 17/39 (43%), Positives = 22/39 (55%), Gaps = 4/39 (10%)
Query: 18 RPVVSEVQA---PFVQQTTPIPQTGLPFPQAAMTYSSPL 53
+P++S QA P QQ P PQ +PF QA + Y PL
Sbjct: 174 QPLLSLPQAKVLPVPQQVVPFPQRDMPF-QALLLYQDPL 211
>YHT1_YEAST (P38835) Hypothetical 95.1 kDa protein in ACT5-YCK1
intergenic region
Length = 840
Score = 29.3 bits (64), Expect = 3.6
Identities = 17/57 (29%), Positives = 24/57 (41%)
Query: 23 EVQAPFVQQTTPIPQTGLPFPQAAMTYSSPLTQNDKESFKTYFKAYAQWWRDFTAQI 79
EV+ P T P P++ LP A Y L E Y + + WR+F +I
Sbjct: 95 EVRFPIFGDTAPCPKSSLPPLYAPAVYELTLISLKLERLSPYEISSNRSWRNFIIEI 151
>MUC2_HUMAN (Q02817) Mucin 2 precursor (Intestinal mucin 2)
Length = 5179
Score = 28.9 bits (63), Expect = 4.8
Identities = 16/48 (33%), Positives = 22/48 (45%)
Query: 9 TPLSSGEVFRPVVSEVQAPFVQQTTPIPQTGLPFPQAAMTYSSPLTQN 56
TP +G P AP + TT P PQ + + SSPLT++
Sbjct: 4187 TPTPTGTQTGPPTHTSTAPIAELTTSNPPPESSTPQTSRSTSSPLTES 4234
>GATB_GLOVI (Q7NI42) Aspartyl/glutamyl-tRNA(Asn/Gln)
amidotransferase subunit B (EC 6.3.5.-) (Asp/Glu-ADT
subunit B)
Length = 554
Score = 28.9 bits (63), Expect = 4.8
Identities = 23/82 (28%), Positives = 35/82 (42%), Gaps = 2/82 (2%)
Query: 74 DFTAQIRPHLEEKEWTKIFLKTLDQFYYDNMVASAPNNFAEMVTMSMHLEEGVREGRLVK 133
D +RP E K TK+ +K L+ F +++ + FA V + E V+E RL
Sbjct: 210 DLNISVRPAGEGKFGTKVEIKNLNSF--NSLQRAVEYEFARQVDCLLSGERIVQETRLWD 267
Query: 134 ENVPTDRSEDEDQEVNMVKRHP 155
E S +E N + P
Sbjct: 268 EATQRTISMRSKEEANDYRYFP 289
>YKF4_YEAST (P35732) Hypothetical 84.0 kDa protein in NUP120-CSE4
intergenic region
Length = 738
Score = 28.1 bits (61), Expect = 8.1
Identities = 17/48 (35%), Positives = 22/48 (45%)
Query: 15 EVFRPVVSEVQAPFVQQTTPIPQTGLPFPQAAMTYSSPLTQNDKESFK 62
EV +P E QQ + +PQ LP P+ +TY S N S K
Sbjct: 68 EVKKPAKKEKYEKKEQQHSYVPQQHLPNPEDDITYKSSNNSNSFTSTK 115
>PTR1_SCHPO (O13834) E3 ubiquitin protein ligase ptr1 (EC 6.3.2.-)
(Poly(A)+ RNA transport protein 1)
Length = 3227
Score = 28.1 bits (61), Expect = 8.1
Identities = 14/67 (20%), Positives = 34/67 (49%)
Query: 75 FTAQIRPHLEEKEWTKIFLKTLDQFYYDNMVASAPNNFAEMVTMSMHLEEGVREGRLVKE 134
F+ + H+ + + +++LD YY ++V N+ +++T +E+ V + V +
Sbjct: 2982 FSRAVYKHMLHRSVSVKDIESLDPDYYKSLVWMLNNDITDIITEEFAVEKDVFGEKTVVD 3041
Query: 135 NVPTDRS 141
+P R+
Sbjct: 3042 LIPNGRN 3048
>NQO1_PARDE (P29913) NADH-quinone oxidoreductase chain 1 (EC
1.6.99.5) (NADH dehydrogenase I, chain 1) (NDH-1, chain
1)
Length = 431
Score = 28.1 bits (61), Expect = 8.1
Identities = 15/44 (34%), Positives = 21/44 (47%), Gaps = 3/44 (6%)
Query: 35 IPQTGLPFPQAAMTYSSPLTQNDKESF---KTYFKAYAQWWRDF 75
+P+ PFP A Y P T N+ ES T + A+W+ F
Sbjct: 189 MPRMKPPFPAGAGLYGCPTTVNNVESIAVVPTILRRGAEWFASF 232
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.131 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,696,486
Number of Sequences: 164201
Number of extensions: 815059
Number of successful extensions: 2303
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2280
Number of HSP's gapped (non-prelim): 32
length of query: 155
length of database: 59,974,054
effective HSP length: 101
effective length of query: 54
effective length of database: 43,389,753
effective search space: 2343046662
effective search space used: 2343046662
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC137994.3