BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137510.8 - phase: 0 /pseudo
(254 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ADPP_BACSU (P54570) ADP-ribose pyrophosphatase (EC 3.6.1.13) (AD... 42 0.002
NUD5_HUMAN (Q9UKK9) ADP-sugar pyrophosphatase (EC 3.6.1.13) (EC ... 41 0.002
NUD5_MOUSE (Q9JKX6) ADP-sugar pyrophosphatase (EC 3.6.1.13) (EC ... 40 0.004
GOA5_MOUSE (Q9QYE6) Golgi autoantigen, golgin subfamily A member... 37 0.035
ADPP_YEAST (Q01976) ADP-ribose pyrophosphatase (EC 3.6.1.13) (AD... 37 0.035
YEAB_ECOLI (P43337) Hypothetical UPF0035 protein yeaB 36 0.10
GTX2_TOBAC (Q03663) Probable glutathione S-transferase (EC 2.5.1... 35 0.23
D250_ASFB7 (P32092) Protein D250R 35 0.23
YEAB_SALTY (P43339) Hypothetical UPF0035 protein yeaB 34 0.39
NUD6_XENLA (P13420) Nucleoside diphosphate-linked moiety X motif... 33 0.51
VP7_BPPH6 (P11123) P7 protein 33 0.87
MUTT_STRAM (P32091) MutT-like protein (ORF154) 33 0.87
YEAB_KLEAE (P43338) Hypothetical UPF0035 protein in pabB-sdaA in... 32 1.1
ASNB_ECOLI (P22106) Asparagine synthetase B [glutamine-hydrolyzi... 32 1.5
DPO2_SULSO (Q07635) DNA polymerase II (EC 2.7.7.7) (DNA polymera... 32 1.9
YFBL_ECOLI (P76482) Hypothetical protein yfbL 31 2.5
LPH_RAT (Q02401) Lactase-phlorizin hydrolase precursor (Lactase-... 31 2.5
NUD6_HUMAN (P53370) Nucleoside diphosphate-linked moiety X motif... 31 3.3
GTX3_TOBAC (Q03664) Probable glutathione S-transferase (EC 2.5.1... 30 4.3
YMFB_ECOLI (P75965) Putative Nudix hydrolase ymfB (EC 3.6.-.-) 30 5.6
>ADPP_BACSU (P54570) ADP-ribose pyrophosphatase (EC 3.6.1.13)
(ADP-ribose diphosphatase) (Adenosine diphosphoribose
pyrophosphatase) (ADPR-PPase) (ADP-ribose
phosphohydrolase) (ASPPase)
Length = 185
Score = 41.6 bits (96), Expect = 0.002
Identities = 26/76 (34%), Positives = 44/76 (57%), Gaps = 5/76 (6%)
Query: 144 PAVAMLILLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGI 203
P ++ + EG+ ++ +Q R P+ R I+E+PAG L+ + + TA+RE+EEETG
Sbjct: 43 PGAVAVLAVTDEGKI--IMVKQFRKPLERTIVEIPAGKLEKGE-EPEYTALRELEEETG- 98
Query: 204 KLNVEDMVDLTAFLDS 219
+ + +TAF S
Sbjct: 99 -YTAKKLTKITAFYTS 113
>NUD5_HUMAN (Q9UKK9) ADP-sugar pyrophosphatase (EC 3.6.1.13) (EC
3.6.1.-) (Nucleoside diphosphate-linked moiety X motif
5) (YSA1H) (HSPC115)
Length = 219
Score = 41.2 bits (95), Expect = 0.002
Identities = 21/49 (42%), Positives = 32/49 (64%), Gaps = 1/49 (2%)
Query: 161 VLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGIKLNVED 209
VL +Q R P+G +E PAG++DD + A+RE+EEETG K ++ +
Sbjct: 78 VLVKQFRPPMGGYCIEFPAGLIDDGETP-EAAALRELEEETGYKGDIAE 125
>NUD5_MOUSE (Q9JKX6) ADP-sugar pyrophosphatase (EC 3.6.1.13) (EC
3.6.1.-) (Nucleoside diphosphate-linked moiety X motif
5)
Length = 218
Score = 40.4 bits (93), Expect = 0.004
Identities = 21/49 (42%), Positives = 30/49 (60%), Gaps = 1/49 (2%)
Query: 161 VLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGIKLNVED 209
+L +Q R P+G LE PAG ++D + A+RE+EEETG K V +
Sbjct: 77 ILVKQFRPPMGSYCLEFPAGFIEDGESP-EAAALRELEEETGYKGEVAE 124
>GOA5_MOUSE (Q9QYE6) Golgi autoantigen, golgin subfamily A member 5
(Sumiko protein) (Ret-II protein)
Length = 729
Score = 37.4 bits (85), Expect = 0.035
Identities = 22/69 (31%), Positives = 35/69 (49%), Gaps = 1/69 (1%)
Query: 37 SSSTESPSLTHSITLPSKQSEPVHILAAPGVSSSDFWSA-IDSSLFKQWLHNLQTENGIL 95
++ ++SP + S ++P EP AP +S + S D S Q L NL+ EN +L
Sbjct: 173 NAGSQSPGVNSSDSVPEVHKEPSEESTAPSATSEEHSSTPSDGSSRSQELSNLRLENQLL 232
Query: 96 ANDTMTLRQ 104
N+ +L Q
Sbjct: 233 RNEVQSLNQ 241
>ADPP_YEAST (Q01976) ADP-ribose pyrophosphatase (EC 3.6.1.13)
(ADP-ribose diphosphatase) (Adenosine diphosphoribose
pyrophosphatase) (ADPR-PPase) (ADP-ribose
phosphohydrolase)
Length = 231
Score = 37.4 bits (85), Expect = 0.035
Identities = 19/42 (45%), Positives = 30/42 (71%), Gaps = 1/42 (2%)
Query: 161 VLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETG 202
+L +Q R PV + +E+PAG++D + DI A+RE++EETG
Sbjct: 97 LLQKQFRPPVEGVCIEMPAGLIDAGE-DIDTAALRELKEETG 137
>YEAB_ECOLI (P43337) Hypothetical UPF0035 protein yeaB
Length = 192
Score = 35.8 bits (81), Expect = 0.10
Identities = 24/84 (28%), Positives = 42/84 (49%), Gaps = 5/84 (5%)
Query: 147 AMLILLESEGETYAVLTEQA---RVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGI 203
A+LI + + +LT+++ R G++ P G +DD + A+RE EEE I
Sbjct: 33 AVLIPIVRRPQPGLLLTQRSIHLRKHAGQVAF--PGGAVDDTDASAIAAALREAEEEVAI 90
Query: 204 KLNVEDMVDLTAFLDSSTGSTVFP 227
+ +++ + +DS TG V P
Sbjct: 91 PPSAVEVIGVLPPVDSVTGYQVTP 114
>GTX2_TOBAC (Q03663) Probable glutathione S-transferase (EC
2.5.1.18) (Auxin-induced protein PGNT35/PCNT111)
Length = 223
Score = 34.7 bits (78), Expect = 0.23
Identities = 24/78 (30%), Positives = 37/78 (46%)
Query: 134 KVPGIVFARGPAVAMLILLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTA 193
KVP ++ P V +I+LE ET+ + + P R + A LDD +V T
Sbjct: 52 KVPVLIHNGKPIVESMIILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVAAVVNTF 111
Query: 194 VREVEEETGIKLNVEDMV 211
R+ EE+ K V +M+
Sbjct: 112 FRKGEEQEKGKEEVYEML 129
>D250_ASFB7 (P32092) Protein D250R
Length = 250
Score = 34.7 bits (78), Expect = 0.23
Identities = 21/53 (39%), Positives = 30/53 (55%), Gaps = 2/53 (3%)
Query: 151 LLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGI 203
LL G+ L QA+ G ++ E+P G +D+ D+ A+RE EEETGI
Sbjct: 104 LLPDNGKKLISLINQAKGS-GTLLWEIPKGKPKEDESDLT-CAIREFEEETGI 154
>YEAB_SALTY (P43339) Hypothetical UPF0035 protein yeaB
Length = 192
Score = 33.9 bits (76), Expect = 0.39
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Query: 147 AMLILLESEGETYAVLTEQA---RVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGI 203
A+LI + + +LT++A R G++ P G +D ++ A+RE +EE I
Sbjct: 33 AVLIPVVRRPQPGLLLTQRAIHLRKHAGQVAF--PGGAVDSTDASLIAAALREAQEEVAI 90
Query: 204 KLNVEDMVDLTAFLDSSTGSTVFP 227
+++ + +DS TG V P
Sbjct: 91 PPQAVEVIGVLPPVDSVTGFQVTP 114
>NUD6_XENLA (P13420) Nucleoside diphosphate-linked moiety X motif 6
(Protein GFG)
Length = 217
Score = 33.5 bits (75), Expect = 0.51
Identities = 27/85 (31%), Positives = 37/85 (42%), Gaps = 6/85 (7%)
Query: 129 KETGNKVPGIVFARGPAVAMLILLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGD 188
K+ +K+PG VA +L E G+ V V + G L D D
Sbjct: 31 KDGPSKLPGYA-THQVGVAGAVLDEDNGKVLVVQDRNKTVNAWKF-----PGGLSDQGED 84
Query: 189 IVGTAVREVEEETGIKLNVEDMVDL 213
I TAVREV EETGI + ++ +
Sbjct: 85 IGATAVREVLEETGIHSEFKSLLSI 109
>VP7_BPPH6 (P11123) P7 protein
Length = 161
Score = 32.7 bits (73), Expect = 0.87
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 3/46 (6%)
Query: 58 PVHILAAPGVSSSDFWSAIDSSL-FKQW--LHNLQTENGILANDTM 100
PV ++A PG S W+ I +L + W L N ++G+LANDT+
Sbjct: 70 PVAVMAVPGASYRSDWNMIAHALPSEDWITLSNKMLKSGLLANDTV 115
>MUTT_STRAM (P32091) MutT-like protein (ORF154)
Length = 154
Score = 32.7 bits (73), Expect = 0.87
Identities = 17/35 (48%), Positives = 25/35 (70%), Gaps = 1/35 (2%)
Query: 176 ELPAGMLDDDKGDIVGTAVREVEEETGIKLNVEDM 210
ELP G+L+ D+ G A REV EETGI++ V+++
Sbjct: 44 ELPGGVLELDETPETGVA-REVWEETGIRVEVDEL 77
>YEAB_KLEAE (P43338) Hypothetical UPF0035 protein in pabB-sdaA
intergenic region (Fragment)
Length = 120
Score = 32.3 bits (72), Expect = 1.1
Identities = 15/50 (30%), Positives = 26/50 (52%)
Query: 178 PAGMLDDDKGDIVGTAVREVEEETGIKLNVEDMVDLTAFLDSSTGSTVFP 227
P G +D+ ++ A+RE +EE I +++ + +DS TG V P
Sbjct: 65 PGGAVDNSDATLIAAALREAQEEVAIPPESVEVIGVLPPVDSVTGFQVTP 114
>ASNB_ECOLI (P22106) Asparagine synthetase B [glutamine-hydrolyzing]
(EC 6.3.5.4)
Length = 553
Score = 32.0 bits (71), Expect = 1.5
Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 1/72 (1%)
Query: 153 ESEGETYAVLTEQARVPVGRIILELPAG-MLDDDKGDIVGTAVREVEEETGIKLNVEDMV 211
+ G+ Y +A VPV R I E PAG L G+I R+ + +K NV D
Sbjct: 150 DEHGQLYVASEMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKN 209
Query: 212 DLTAFLDSSTGS 223
+L L+ S S
Sbjct: 210 ELRQALEDSVKS 221
>DPO2_SULSO (Q07635) DNA polymerase II (EC 2.7.7.7) (DNA polymerase
B2)
Length = 626
Score = 31.6 bits (70), Expect = 1.9
Identities = 38/149 (25%), Positives = 67/149 (44%), Gaps = 16/149 (10%)
Query: 71 DFWSAIDSSLFKQWLHNLQTENGILANDTMT-LRQVLIQGVDMFGKRIGFL---KFIAEI 126
DF S S + K HN+ E +D T L + ++ + + + +L K +
Sbjct: 344 DFSSMYPSLIVK---HNISAETVEACDDIKTELHSICLKEKGIIPEALQWLIERKSELKK 400
Query: 127 IDKETGNKVPGIVFARGPAVAMLILLESEGETYAVLTEQARVPVGRII-------LELPA 179
ID+E + I+ A + L + E Y ++T AR + R + L +
Sbjct: 401 IDEERAEAIKWILVASFGYLGYRNSLFGKIEAYEMVTYLARKTLRRTMEIAEEMGLRVLH 460
Query: 180 GMLDD--DKGDIVGTAVREVEEETGIKLN 206
G++D KGD V + +VE+ETG++L+
Sbjct: 461 GIIDSLVVKGDNVDKFIEKVEKETGLRLD 489
>YFBL_ECOLI (P76482) Hypothetical protein yfbL
Length = 323
Score = 31.2 bits (69), Expect = 2.5
Identities = 18/67 (26%), Positives = 34/67 (49%), Gaps = 2/67 (2%)
Query: 186 KGDIVGTAVREVEEETGIKLNVEDMVDLTAFLDSSTGSTVFPSPSAESVLIKRSSHISKV 245
+ D +G+AV E +KL + +++ + DS+ GS +P P+ + R I+ V
Sbjct: 171 RSDEMGSAVHAASLERPVKLMIA--LEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIAVV 228
Query: 246 KRLAFVN 252
R+ +N
Sbjct: 229 GRIQDIN 235
>LPH_RAT (Q02401) Lactase-phlorizin hydrolase precursor
(Lactase-glycosylceramidase) [Includes: Lactase (EC
3.2.1.108); Phlorizin hydrolase (EC 3.2.1.62)]
Length = 1928
Score = 31.2 bits (69), Expect = 2.5
Identities = 17/48 (35%), Positives = 27/48 (55%), Gaps = 2/48 (4%)
Query: 30 NGFFYKMSSSTESPS--LTHSITLPSKQSEPVHILAAPGVSSSDFWSA 75
N F S+S ESPS LT S+ L ++Q + + ++PG + W+A
Sbjct: 320 NAFLSCTSNSEESPSCSLTDSLALQTEQQQETAVPSSPGSAYQRVWAA 367
>NUD6_HUMAN (P53370) Nucleoside diphosphate-linked moiety X motif 6
(Protein GFG) (GFG-1) (Antisense basic fibroblast growth
factor)
Length = 316
Score = 30.8 bits (68), Expect = 3.3
Identities = 16/34 (47%), Positives = 21/34 (61%)
Query: 180 GMLDDDKGDIVGTAVREVEEETGIKLNVEDMVDL 213
G L + + DI TAVREV EETGIK ++ +
Sbjct: 175 GGLSEPEEDIGDTAVREVFEETGIKSEFRSVLSI 208
>GTX3_TOBAC (Q03664) Probable glutathione S-transferase (EC
2.5.1.18) (Auxin-induced protein PCNT103)
Length = 223
Score = 30.4 bits (67), Expect = 4.3
Identities = 21/78 (26%), Positives = 36/78 (45%)
Query: 134 KVPGIVFARGPAVAMLILLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTA 193
KVP ++ P V +++LE ET+ + + P R + + L D +V T
Sbjct: 52 KVPVLIHNGKPIVESMVILEYIDETFEGPSILPKDPYDRALARFWSKFLGDKVAAVVNTF 111
Query: 194 VREVEEETGIKLNVEDMV 211
R+ EE+ K V +M+
Sbjct: 112 FRKGEEQEKGKEEVYEML 129
>YMFB_ECOLI (P75965) Putative Nudix hydrolase ymfB (EC 3.6.-.-)
Length = 153
Score = 30.0 bits (66), Expect = 5.6
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Query: 144 PAVAMLILLESEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGI 203
P V + ++ +EG+ + V+ E G+ + PAG L+ D+ +V A RE+ EETGI
Sbjct: 4 PHVTVACVVHAEGK-FLVVEETIN---GKALWNQPAGHLEADE-TLVEAAARELWEETGI 58
Query: 204 KLNVEDMV 211
+ +
Sbjct: 59 SAQPQHFI 66
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,293,125
Number of Sequences: 164201
Number of extensions: 1172908
Number of successful extensions: 3387
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 3373
Number of HSP's gapped (non-prelim): 26
length of query: 254
length of database: 59,974,054
effective HSP length: 108
effective length of query: 146
effective length of database: 42,240,346
effective search space: 6167090516
effective search space used: 6167090516
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC137510.8