BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC136287.1 + phase: 1 /partial
         (436 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

MTHR_ARATH (O80585) Methylenetetrahydrofolate reductase (EC 1.5....   739  0.0
MTHR_HUMAN (P42898) Methylenetetrahydrofolate reductase (EC 1.5....   396  e-110
MTHR_MOUSE (Q9WU20) Methylenetetrahydrofolate reductase (EC 1.5....   383  e-106
MTHR_CAEEL (Q17693) Probable methylenetetrahydrofolate reductase...   357  3e-98
MTHR_SCHPO (Q10258) Methylenetetrahydrofolate reductase 1 (EC 1....   343  4e-94
MTHS_YEAST (P53128) Methylenetetrahydrofolate reductase 2 (EC 1....   307  4e-83
MTHS_SCHPO (O74927) Methylenetetrahydrofolate reductase 2 (EC 1....   242  2e-63
MTHR_YEAST (P46151) Methylenetetrahydrofolate reductase 1 (EC 1....   173  7e-43
METF_AQUAE (O67422) 5,10-methylenetetrahydrofolate reductase (EC...   105  2e-22
METF_ECOLI (P00394) 5,10-methylenetetrahydrofolate reductase (EC...    98  5e-20
METF_HAEIN (P45208) 5,10-methylenetetrahydrofolate reductase (EC...    97  8e-20
METF_SALTY (P11003) 5,10-methylenetetrahydrofolate reductase (EC...    96  2e-19
METF_ERWCA (P71319) 5,10-methylenetetrahydrofolate reductase (EC...    92  2e-18
METF_BUCAI (P57154) 5,10-methylenetetrahydrofolate reductase (EC...    88  5e-17
METF_STRLI (O54235) 5,10-methylenetetrahydrofolate reductase (EC...    87  1e-16
METF_BUCBP (Q89B13) 5,10-methylenetetrahydrofolate reductase (EC...    84  7e-16
METF_BUCAP (Q8KA62) 5,10-methylenetetrahydrofolate reductase (EC...    81  5e-15
DSC1_MOUSE (P55849) Desmocollin 1A/1B precursor                        36  0.22
SYK_CHLTR (O84786) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--t...    35  0.38
SYK_CHLMU (Q9PLE1) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--t...    35  0.38

>MTHR_ARATH (O80585) Methylenetetrahydrofolate reductase (EC
           1.5.1.20) (MTHFR2)
          Length = 594

 Score =  739 bits (1907), Expect = 0.0
 Identities = 346/433 (79%), Positives = 388/433 (88%)

Query: 1   AHPDVIGADGLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITC 60
           AHPDVIG +GLA+ E YQSDL YLK K+DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+C
Sbjct: 156 AHPDVIGENGLASNEAYQSDLEYLKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISC 215

Query: 61  PIVPGIMPINNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIM 120
           PIVPGIMPINNY+GF+RMTGFCKTKIP E+MAALEPIKDNEEAVKAYGIHLGTEMCKK++
Sbjct: 216 PIVPGIMPINNYRGFLRMTGFCKTKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKML 275

Query: 121 AHGIKTVHLYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANR 180
           AHG+K++HLYTLNMEKSALAIL +LG+I+ESKISRSLPWRRP NVFR KEDVRPIFWANR
Sbjct: 276 AHGVKSLHLYTLNMEKSALAILMNLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANR 335

Query: 181 PKSYISRTIGWEQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIY 240
           PKSYISRT GWE +P GRW DS + SYGAL+D+QF RPRA+DKKL +EW VPLKS+EDI 
Sbjct: 336 PKSYISRTKGWEDFPQGRWGDSRSASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQ 395

Query: 241 ERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIV 300
           E+F+  CLG L+++PWSELDGLQPET+IINE L K+N+KGFLTINSQP+VN E+SDSP V
Sbjct: 396 EKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTV 455

Query: 301 GWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNAV 360
           GWGGP GY+YQKAYLEFFCSKEKLDA++EK K  P +TYMAVNK   W SN  Q DVNAV
Sbjct: 456 GWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAV 515

Query: 361 TWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLV 420
           TWGVFPAKEIIQPTIVDP SFNVWKDEAFE WSR WA+LYPEAD SR L+EEV +SY+LV
Sbjct: 516 TWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLV 575

Query: 421 SLVDNDYANGDLF 433
           SLV+NDY NGD+F
Sbjct: 576 SLVENDYINGDIF 588


>MTHR_HUMAN (P42898) Methylenetetrahydrofolate reductase (EC
           1.5.1.20)
          Length = 656

 Score =  396 bits (1017), Expect = e-110
 Identities = 195/438 (44%), Positives = 278/438 (62%), Gaps = 13/438 (2%)

Query: 10  GLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPI 69
           G   A  +++DL +LK KV AGAD I+TQLF++ D F +FV  C  +GITCPIVPGI PI
Sbjct: 200 GHPEAGSFEADLKHLKEKVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPI 259

Query: 70  NNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHG-IKTVH 128
             Y    ++    K ++P EI   +EPIKDN+ A++ YGI L   +C++++A G +  +H
Sbjct: 260 QGYHSLRQLVKLSKLEVPQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLH 319

Query: 129 LYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANRPKSYISRT 188
            YTLN E +   +L  LG+  E    R LPW    +  R +EDVRPIFWA+RPKSYI RT
Sbjct: 320 FYTLNREMATTEVLKRLGMWTEDP-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRT 378

Query: 189 IGWEQYPHGRWSDSGNPSYGALTDYQ--FMRPRAKDKKLIEEWAVPLKSIEDIYERFRLF 246
             W+++P+GRW +S +P++G L DY   +++ ++  ++L++ W   L S   ++E F L+
Sbjct: 379 QEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFVLY 438

Query: 247 CLGKLRTN-------PWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPI 299
             G+   N       PW++ + L  ET ++ E L ++N +G LTINSQP +NG+ S  PI
Sbjct: 439 LSGEPNRNGHKVTCLPWND-EPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPI 497

Query: 300 VGWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPF-LTYMAVNKEGIWKSNVGQTDVN 358
           VGWG  GGY++QKAYLEFF S+E  +AL++  K     + Y  VN +G   +N  +   N
Sbjct: 498 VGWGPSGGYVFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPN 557

Query: 359 AVTWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYF 418
           AVTWG+FP +EIIQPT+VDPVSF  WKDEAF +W   W  LY E   SR +++ +  +YF
Sbjct: 558 AVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYF 617

Query: 419 LVSLVDNDYANGDLFWCV 436
           LV+LVDND+   +  W V
Sbjct: 618 LVNLVDNDFPLDNCLWQV 635


>MTHR_MOUSE (Q9WU20) Methylenetetrahydrofolate reductase (EC
           1.5.1.20)
          Length = 654

 Score =  383 bits (984), Expect = e-106
 Identities = 186/438 (42%), Positives = 275/438 (62%), Gaps = 13/438 (2%)

Query: 10  GLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPI 69
           G   AE ++ DL +LK KV AGAD I+TQLF++   F  FV  C +IGI+CPI+PGI PI
Sbjct: 199 GHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPI 258

Query: 70  NNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHG-IKTVH 128
             Y    ++    K ++P +I   +EPIKDN+ A++ YGI L   +C++++  G +  +H
Sbjct: 259 QGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVRLCRELLDSGLVPGLH 318

Query: 129 LYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANRPKSYISRT 188
            YTLN E + + +L  LG+  E    R LPW    +  R +EDVRPIFWA+RPKSYI RT
Sbjct: 319 FYTLNREVATMEVLKQLGMWTEDP-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRT 377

Query: 189 IGWEQYPHGRWSDSGNPSYGALTDYQ--FMRPRAKDKKLIEEWAVPLKSIEDIYERFRLF 246
             W+++P+GRW +S +P++G L DY   +++ ++  ++L++ W   L S E ++E F  +
Sbjct: 378 QDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHY 437

Query: 247 CLG-------KLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPI 299
             G       ++   PW++ + L  ET ++ E L ++N  G LTINSQP +N + S  P+
Sbjct: 438 LSGEPNRHGYRVTCLPWND-EPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPV 496

Query: 300 VGWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPF-LTYMAVNKEGIWKSNVGQTDVN 358
           VGWG  GGY++QKAYLEFF S+E ++AL++  K     + Y  V+ +G   +N  +   N
Sbjct: 497 VGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPN 556

Query: 359 AVTWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYF 418
           AVTWG+FP +EIIQPT+VDP+SF  WKDEAF +W   W  LY E   SR +++ +  +YF
Sbjct: 557 AVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYF 616

Query: 419 LVSLVDNDYANGDLFWCV 436
           LV+LVDN++      W V
Sbjct: 617 LVNLVDNEFPLDSCLWQV 634


>MTHR_CAEEL (Q17693) Probable methylenetetrahydrofolate reductase
           (EC 1.5.1.20)
          Length = 663

 Score =  357 bits (916), Expect = 3e-98
 Identities = 191/435 (43%), Positives = 273/435 (61%), Gaps = 20/435 (4%)

Query: 10  GLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPI 69
           G   A  Y++DL YLK+K DAGA+ ++TQLF++ + F KFV DCR+IGIT PI+PGIMPI
Sbjct: 213 GHPQAPSYKADLMYLKAKCDAGANFVITQLFFEAETFEKFVRDCREIGITQPIIPGIMPI 272

Query: 70  NNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHG-IKTVH 128
             Y+   R+    + +IP  I+  LEPIK +++AV+ YG     EMC++++ +G   ++H
Sbjct: 273 MGYESIKRIAKLSQLEIPQHILDDLEPIKHDDDAVQKYGTERCIEMCRRLLDNGTAPSIH 332

Query: 129 LYTLNMEKSALAILTSLGLIEESKISRSLPWR-RPTNVFRVKEDVRPIFWANRPKSYISR 187
           LYT+N E S   IL SLGL  + +  R  PW+ R  +  R  E VRPI+W+ RP+SYI+R
Sbjct: 333 LYTMNREGSIREILKSLGL-WKLEGDRVFPWKNRSQHPIRCLESVRPIYWSFRPRSYITR 391

Query: 188 TIGWEQYPHGRWSDSGNPSYGALTDY---QFMRPRAKDKKLIEEWAVPLKSIEDIYERFR 244
           T  W+Q+P+GRW +S +P++G ++ Y        R  D +L   +   ++S ED+   F 
Sbjct: 392 TRDWDQFPNGRWGNSSSPAFGDVSSYYLSNLTTVRNADDRL-AMFGANIESFEDVKRVFI 450

Query: 245 LFCLG-------KLRTNPWSELD-GLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSD 296
            +          K+   PW+E + G+QPET +I+E L   N  G LT+NSQP+VNG  S 
Sbjct: 451 NYITQAPNADGVKVTVLPWTEAETGVQPETSLISEQLVWCNENGILTVNSQPSVNGAPST 510

Query: 297 SPIVGWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPF---LTYMAVNKEGIW-KSNV 352
            P+VGWG PGGY YQKAYLE F + E  D LI+   +R F   + Y A+NK+  + K+N 
Sbjct: 511 DPLVGWGKPGGYCYQKAYLECFMTAELSDKLIQ-IIEREFPVRVNYHAINKDSTFDKTNS 569

Query: 353 GQTDVNAVTWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEE 412
            +T   AVTWGVFP  EI QPT+VDP+SF  W+DEA+++W   W   YP+   S  +++ 
Sbjct: 570 EETTPIAVTWGVFPGSEIAQPTVVDPLSFRAWRDEAYQMWMAQWGDFYPKESKSYGVIKA 629

Query: 413 VGSSYFLVSLVDNDY 427
           V   + LV+LVDND+
Sbjct: 630 VHDEFRLVTLVDNDF 644


>MTHR_SCHPO (Q10258) Methylenetetrahydrofolate reductase 1 (EC
           1.5.1.20)
          Length = 603

 Score =  343 bits (881), Expect = 4e-94
 Identities = 186/431 (43%), Positives = 251/431 (58%), Gaps = 23/431 (5%)

Query: 15  EGYQSD------LAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMP 68
           EGY  D      + +LK KVD GAD IVTQ+FYD D F+ +V+  R  GI  PI PGIMP
Sbjct: 155 EGYSPDDDIDESIKHLKLKVDEGADFIVTQMFYDVDNFIAWVDKVRAAGINIPIFPGIMP 214

Query: 69  INNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVH 128
           I  +  FIR   +   KIP   M  L P+KD++E V+  G+ L  EMC+K++A GI  +H
Sbjct: 215 IQAWDSFIRRAKWSGVKIPQHFMDTLVPVKDDDEGVRERGVELIVEMCRKLIASGITRLH 274

Query: 129 LYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVF---------RVKEDVRPIFWAN 179
            YT+N+EK+   I+  LGL++E+      P     NV          R+ E VRPIFW  
Sbjct: 275 FYTMNLEKAVKMIIERLGLLDENLA----PIVDTNNVELTNASSQDRRINEGVRPIFWRT 330

Query: 180 RPKSYISRTIGWEQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDI 239
           R +SY+SRT  W++ PHGRW DS +P++G     ++   R   K++   W  P KS  +I
Sbjct: 331 RNESYVSRTDQWDELPHGRWGDSRSPAFGEFDAIRYGL-RMSPKEITTSWGSP-KSYSEI 388

Query: 240 YERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPI 299
            + F  +C  K+ + PWS+L  +  E  +I + L  +N   FLTINSQPA+NGEKS  P+
Sbjct: 389 GDLFARYCEKKISSLPWSDLP-ISDEADLIRDQLLSMNRNAFLTINSQPALNGEKSSHPV 447

Query: 300 VGWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNA 359
            GWG P GY++QK Y+EFF     L+ L E  K    ++Y   NK G   +N      NA
Sbjct: 448 FGWGPPNGYVFQKPYVEFFVHPSLLNELKETVKKLNSVSYFVTNKNGDLDTNSQYEIPNA 507

Query: 360 VTWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFL 419
           VTWGVFP +EIIQPTIV+  SF  WKDEA+ +    WA+ Y     SRKL+  +   +FL
Sbjct: 508 VTWGVFPNREIIQPTIVESTSFLAWKDEAYSL-GMEWANAYSPDSISRKLLVSMMKEWFL 566

Query: 420 VSLVDNDYANG 430
             +VDND+ NG
Sbjct: 567 CVIVDNDFQNG 577


>MTHS_YEAST (P53128) Methylenetetrahydrofolate reductase 2 (EC
           1.5.1.20)
          Length = 599

 Score =  307 bits (786), Expect = 4e-83
 Identities = 172/423 (40%), Positives = 249/423 (58%), Gaps = 15/423 (3%)

Query: 20  DLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMT 79
           DL YL S+   G D I+TQ+FYD D  L + +  R  G+  PI+PGIMPI  Y  F+R  
Sbjct: 171 DLEYL-SRRSTGGDFIITQMFYDVDNLLNWCSQVRAAGMDVPIIPGIMPITTYAAFLRRI 229

Query: 80  GFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHG-IKTVHLYTLNMEKSA 138
            + +  IP    + L+PIKD++E V+  G +L  EMC+K++  G +  +H+YT+N+EK+ 
Sbjct: 230 QWGQISIPQHFSSRLDPIKDDDELVRDIGTNLIVEMCQKLLDSGYVSHLHIYTMNLEKAP 289

Query: 139 LAILTSLGLI-EESKISRS----LPWRRPTNVFRVKEDVRPIFWANRPKSYISRTIGW-- 191
           L IL  L ++  ES+ +      LPWR+  N  R  E+VRPIFW  RP SY++RT  W  
Sbjct: 290 LMILERLNILPTESEFNAHPLAVLPWRKSLNPKRKNEEVRPIFWKRRPYSYVARTSQWAV 349

Query: 192 EQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIYERFRLFCLGKL 251
           +++P+GR+ DS +P++G L        R    K +E W+ P  SI D+      +  G L
Sbjct: 350 DEFPNGRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTP-TSINDVAFLVINYLNGNL 408

Query: 252 RTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIVGWGGPGGYIYQ 311
           +  PWS++  +  E   I  HL ++N    +TINSQP VNG +S+  I GWG   GY+YQ
Sbjct: 409 KCLPWSDIP-INDEINPIKAHLIELNQHSIITINSQPQVNGIRSNDKIHGWGPKDGYVYQ 467

Query: 312 KAYLEFFCSKEKLDALIEKSKDRPFLTYMAVNKEGIWKSN-VGQTDVNAVTWGVFPAKEI 370
           K YLEF   K KL  LI+  K+  FLTY A++ +G   SN    +  NAVTWG+FP +EI
Sbjct: 468 KQYLEFMLPKTKLPKLIDTLKNNEFLTYFAIDSQGDLLSNHPDNSKSNAVTWGIFPGREI 527

Query: 371 IQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADA--SRKLVEEVGSSYFLVSLVDNDYA 428
           +QPTIV+ +SF  WK+E + I +  W     + D   S + ++ +   Y LV++VDNDY 
Sbjct: 528 LQPTIVEKISFLAWKEEFYHILNE-WKLNMNKYDKPHSAQFIQSLIDDYCLVNIVDNDYI 586

Query: 429 NGD 431
           + D
Sbjct: 587 SPD 589


>MTHS_SCHPO (O74927) Methylenetetrahydrofolate reductase 2 (EC
           1.5.1.20)
          Length = 641

 Score =  242 bits (617), Expect = 2e-63
 Identities = 153/474 (32%), Positives = 239/474 (50%), Gaps = 66/474 (13%)

Query: 20  DLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITC----PIVPGIMPINNYKGF 75
           D+ +L  KV+AGAD I+TQ+FY+ + F+KF N  R          PI+P IMPI +Y   
Sbjct: 172 DIPFLIEKVEAGADFIITQIFYEPEAFIKFENFVRNHSSNALRNIPIIPAIMPIQSYGSL 231

Query: 76  IRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIM------AHGIKTVHL 129
            RMT  C   +P+ +M  L   K ++EA+K  G+    +M KKIM       HG    H 
Sbjct: 232 KRMTRLCGCSVPSSLMQRLNAAKPDDEAIKNIGVEHIVDMIKKIMDNVQGRVHGF---HF 288

Query: 130 YTLNMEKSALAILTSLGLI-----------EESKISRS------------------LPWR 160
            TLN+E+S   IL + GL+           E+ K+ R+                  +P +
Sbjct: 289 CTLNLERSVALILKNSGLLTKRWKQVESEMEDEKLLRTTRKRLSLDEPAELHNQVVVPSQ 348

Query: 161 RP-----TNVFRVKEDV-----------RPIFWANRPKSYISRTIGWEQYPHGRWSDSGN 204
           +P     +N+F   +                F  +     + R   W+ + +GR+ D  +
Sbjct: 349 QPVADKSSNLFVTSKQSSVSGHKDNLTEEAPFSVSEGSGVLGRQANWDDFTNGRFGDPRS 408

Query: 205 PSYGALTDY--QFMRPRAKDKKLIEEWAVPLKSIEDIYERFRLFCLGKLRTNPWSELDGL 262
           P+YG +  Y      P ++  KL   W  P+    DI   F+   +  +   PW + + +
Sbjct: 409 PAYGEIDGYGPTLHFPPSEALKL---WGYPVDE-SDITSLFQKHIMSDISAIPWID-EPV 463

Query: 263 QPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIVGWGGPGGYIYQKAYLEFFCSKE 322
           + ETK I ++L K+N   + T+ SQPAVNG  S  P+ GWG  GG ++QKA++E F + +
Sbjct: 464 EVETKTIAKYLLKLNGNSWWTVGSQPAVNGAPSADPVFGWGPKGGRVFQKAFVECFVNGK 523

Query: 323 KLDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFN 382
            L   I K  D P +TY A N +  + +N  +   +AVTWGV+P +EIIQ TI+  VSF 
Sbjct: 524 DLKDFITKWHDNPQVTYYAGNNKSEFLTNAPKDGASAVTWGVYPGREIIQSTIIAEVSFK 583

Query: 383 VWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLVSLVDNDYANGDLFWCV 436
            W  E+F++W   WA+LY +   SRKL+E   +  +LV+++ +D+ + +  W V
Sbjct: 584 AWLSESFQVWGE-WANLYSKNTPSRKLLENCINDRWLVTVIHHDFMDKNGLWKV 636


>MTHR_YEAST (P46151) Methylenetetrahydrofolate reductase 1 (EC
           1.5.1.20)
          Length = 657

 Score =  173 bits (439), Expect = 7e-43
 Identities = 94/253 (37%), Positives = 142/253 (55%), Gaps = 7/253 (2%)

Query: 185 ISRTIGWEQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIYERFR 244
           + R   W+++P+GR+ DS +P+YG +  Y      +K K L E W +P K+I D+ + F 
Sbjct: 402 LGRDATWDEFPNGRFGDSRSPAYGEIDGYGPSIKVSKSKAL-ELWGIP-KTIGDLKDIFI 459

Query: 245 LFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIVGWGG 304
            +  G     PWS+L GL  ET +I E L ++N +G+LT+ SQPA N   S   I GWG 
Sbjct: 460 KYLEGSTDAIPWSDL-GLSAETALIQEELIQLNYRGYLTLASQPATNATLSSDKIFGWGP 518

Query: 305 PGGYIYQKAYLEFFCSKEKLDALIEKSKD---RPFLTYMAVNKEGIWKSNVGQTDVNAVT 361
             G +YQKA++E F  +++ +  ++   D   R   +Y A +  G +++N+     + VT
Sbjct: 519 AKGRLYQKAFVEMFIHRQQWETTLKPKLDHYGRRKFSYYAGDSSGSFETNLDPHSSSVVT 578

Query: 362 WGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLVS 421
           WGVFP   + Q TI++  SF  W+DEAF IWS  WA L+P    +  L+  V   Y LVS
Sbjct: 579 WGVFPNSPVKQTTIIEEESFKAWRDEAFSIWSE-WAKLFPRNTPANILLRLVHKDYCLVS 637

Query: 422 LVDNDYANGDLFW 434
           +V +D+   D  W
Sbjct: 638 IVHHDFKETDELW 650



 Score = 92.8 bits (229), Expect = 2e-18
 Identities = 56/139 (40%), Positives = 82/139 (58%), Gaps = 9/139 (6%)

Query: 14  AEGYQSD----LAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQ-IGITCPIVPGIMP 68
           AEG++ D    L YLK KV+AGAD ++TQLFYD + FL F    R+ I    P+ PG+MP
Sbjct: 161 AEGHEQDPLKDLVYLKEKVEAGADFVITQLFYDVEKFLTFEMLFRERISQDLPLFPGLMP 220

Query: 69  INNYKGFIRMTGFCKTKIPAEIMAALEP-IKDNEEAVKAYGIHLGTEMCKKI---MAHGI 124
           IN+Y  F R        IP  I++   P I+ ++ AVK+ G+ +  E+ ++I    +  I
Sbjct: 221 INSYLLFHRAAKLSHASIPPAILSRFPPEIQSDDNAVKSIGVDILIELIQEIYQRTSGRI 280

Query: 125 KTVHLYTLNMEKSALAILT 143
           K  H YTLN+EK+   I++
Sbjct: 281 KGFHFYTLNLEKAIAQIVS 299


>METF_AQUAE (O67422) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 296

 Score =  105 bits (263), Expect = 2e-22
 Identities = 48/137 (35%), Positives = 74/137 (53%)

Query: 9   DGLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMP 68
           +G   +   + ++ Y K KV+AGAD  +TQ+F+  D + +FV  C+  GI   I+PGIMP
Sbjct: 151 EGHPESPNLEWEVKYFKEKVEAGADFSITQMFFVNDYYYRFVEMCKNAGIDISIIPGIMP 210

Query: 69  INNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVH 128
           I N+K   +    C   IP  ++  LE ++D  E VK  GI      C  ++ HG+  +H
Sbjct: 211 ITNFKQIRKFASLCGATIPQSLIEKLEKVEDKPEEVKKIGIEFAINQCLDLIEHGVPGLH 270

Query: 129 LYTLNMEKSALAILTSL 145
            YTLN   + L I  ++
Sbjct: 271 FYTLNKSDATLKIYEAI 287


>METF_ECOLI (P00394) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 296

 Score = 97.8 bits (242), Expect = 5e-20
 Identities = 48/134 (35%), Positives = 77/134 (56%)

Query: 14  AEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYK 73
           A+  Q+DL  LK KVDAGA+  +TQ F+D + +L+F + C   GI   I+PGI+P++N+K
Sbjct: 159 AKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK 218

Query: 74  GFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLN 133
              +       +IPA +    + + D+ E  K  G ++  +M K +   G+K  H YTLN
Sbjct: 219 QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLN 278

Query: 134 MEKSALAILTSLGL 147
             + + AI  +LG+
Sbjct: 279 RAEMSYAICHTLGV 292


>METF_HAEIN (P45208) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 292

 Score = 97.1 bits (240), Expect = 8e-20
 Identities = 48/134 (35%), Positives = 75/134 (55%)

Query: 14  AEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYK 73
           A+  Q+DL  LK K+DAGA+ ++TQ F+D + +L+F + C  IGI   IVPGI+P+ N+K
Sbjct: 157 AKSAQADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFK 216

Query: 74  GFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLN 133
              +M  F   KIPA ++ A + + ++          +  +M K +   G+   H YTLN
Sbjct: 217 QLQKMASFTNVKIPAWLVKAYDGLDNDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLN 276

Query: 134 MEKSALAILTSLGL 147
             +   AI   LG+
Sbjct: 277 RSELTYAICHMLGV 290


>METF_SALTY (P11003) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 296

 Score = 95.9 bits (237), Expect = 2e-19
 Identities = 47/134 (35%), Positives = 77/134 (57%)

Query: 14  AEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYK 73
           A+  Q+DL  LK KVDAGA+  +TQ F+D + +L+F + C   GI   I+PGI+P++N+K
Sbjct: 159 AKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK 218

Query: 74  GFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLN 133
              +       +IP+ +    E + ++ E  K  G ++  +M K +   G+K  H YTLN
Sbjct: 219 QAKKFADMTNVRIPSWMSLMFEGLDNDAETRKLVGANIAMDMVKILSREGVKDFHFYTLN 278

Query: 134 MEKSALAILTSLGL 147
             + + AI  +LG+
Sbjct: 279 RAEMSYAICHTLGV 292


>METF_ERWCA (P71319) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 298

 Score = 92.4 bits (228), Expect = 2e-18
 Identities = 45/134 (33%), Positives = 75/134 (55%)

Query: 14  AEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYK 73
           A+  Q+DL  LK K+DAGA+  +TQ F+D + +L+F + C   GI   IVPGI+P++N+K
Sbjct: 159 AKSAQADLINLKHKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFK 218

Query: 74  GFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLN 133
              +       ++P  +    + + ++ E  K  G  +  +M K +   G+K  H YTLN
Sbjct: 219 QLQKFATMTNVRVPNWMTTMFDGLDNDPETRKMVGASIAMDMVKILSREGVKDFHFYTLN 278

Query: 134 MEKSALAILTSLGL 147
             + + AI  +LG+
Sbjct: 279 RAELSYAICHTLGV 292


>METF_BUCAI (P57154) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 292

 Score = 87.8 bits (216), Expect = 5e-17
 Identities = 44/134 (32%), Positives = 72/134 (52%)

Query: 14  AEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYK 73
           ++  +SD+  LK KVDAGA   +TQ F++ + +L+F ++C +  I   I+PGI+P+ N++
Sbjct: 159 SKNVKSDILNLKKKVDAGASRAITQFFFNIESYLRFRDNCIKNKINIDIIPGILPVCNFQ 218

Query: 74  GFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLN 133
              R +     KIP  ++     + D+    K  G  +  +M KK+   G+K  H YTLN
Sbjct: 219 KLKRFSSMTNVKIPKWMLDMFNGLDDDIFTQKIIGSSIAIDMVKKLSCEGVKNFHFYTLN 278

Query: 134 MEKSALAILTSLGL 147
                 +I   LGL
Sbjct: 279 QSDITYSICHILGL 292


>METF_STRLI (O54235) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 307

 Score = 86.7 bits (213), Expect = 1e-16
 Identities = 41/131 (31%), Positives = 68/131 (51%)

Query: 17  YQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFI 76
           + +D+     K  AGAD  +TQ+F+  D +L+  +     G   P++P +MP+ + K   
Sbjct: 171 WDTDVTNFVDKCRAGADYAITQMFFQPDSYLRLRDRVAAAGCATPVIPEVMPVTSVKMLE 230

Query: 77  RMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLNMEK 136
           R+        PAE+   +   KD+  AV++ GI   TE C +++A G+  +H  TLN   
Sbjct: 231 RLPKLSNASFPAELKERILTAKDDPAAVRSIGIEFATEFCARLLAEGVPGLHFITLNNST 290

Query: 137 SALAILTSLGL 147
           + L I  +LGL
Sbjct: 291 ATLEIYENLGL 301


>METF_BUCBP (Q89B13) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 295

 Score = 84.0 bits (206), Expect = 7e-16
 Identities = 44/140 (31%), Positives = 73/140 (51%), Gaps = 9/140 (6%)

Query: 2   HPDVIGADGLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCP 61
           HP+ + A         + D+  LK KV+AGA   +TQ F++ D FL+F + C +  IT  
Sbjct: 157 HPEAVNA---------KYDIINLKRKVEAGATRAITQFFFNIDCFLRFRDLCVKNNITID 207

Query: 62  IVPGIMPINNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMA 121
           IVPGI PI+N+K  ++ +      IP  +      + ++    +  G  +  +M K + +
Sbjct: 208 IVPGIFPISNFKQLLKFSSVSNVSIPKWLCCMFHGLDNDLNTSRIIGSSIAIDMVKVLYS 267

Query: 122 HGIKTVHLYTLNMEKSALAI 141
            GI++ H YTLN  + + AI
Sbjct: 268 EGIRSFHFYTLNKSEISFAI 287


>METF_BUCAP (Q8KA62) 5,10-methylenetetrahydrofolate reductase (EC
           1.7.99.5)
          Length = 292

 Score = 81.3 bits (199), Expect = 5e-15
 Identities = 40/130 (30%), Positives = 68/130 (51%)

Query: 18  QSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIR 77
           QSD+  LK K DAGA+  +TQ F++ + +L+F ++C +  I   I+PGI+PI N++   +
Sbjct: 163 QSDILNLKKKADAGANRAITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKK 222

Query: 78  MTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLNMEKS 137
            +      IP  +      + ++ E  K  G ++   + K ++  G+K  H YTLN    
Sbjct: 223 FSSMTNVSIPKWMFEMFNGLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDV 282

Query: 138 ALAILTSLGL 147
             +I    GL
Sbjct: 283 IYSICHMFGL 292


>DSC1_MOUSE (P55849) Desmocollin 1A/1B precursor
          Length = 886

 Score = 35.8 bits (81), Expect = 0.22
 Identities = 32/113 (28%), Positives = 51/113 (44%), Gaps = 8/113 (7%)

Query: 86  IPAEIMAALEPIKDN-EEAVKAYGIHLGTEMCKKIMAHG-IKTVHLYTLNMEKSALAILT 143
           +P+ + A     K N EE +K+  + L ++   +I+  G I T H   L+ EK   +IL 
Sbjct: 37  VPSHLKAETPVGKVNLEECLKSPSLILSSDPAFRILEDGTIYTTHDLLLSSEKRGFSILL 96

Query: 144 SLGLIEESKISRSLPWRRPTNVFRVKEDVRPIF------WANRPKSYISRTIG 190
           S G  +E K    +   R   VFR +    P+       WA  P S +  ++G
Sbjct: 97  SDGQGQEQKKLEVVLSAREKKVFRKRHTKEPVHNRSKRRWAPIPCSLMENSLG 149


>SYK_CHLTR (O84786) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA
           ligase) (LysRS)
          Length = 526

 Score = 35.0 bits (79), Expect = 0.38
 Identities = 16/39 (41%), Positives = 23/39 (58%)

Query: 237 EDIYERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEK 275
           E + ERF  FCLGK   N +SEL+    + K++ E + K
Sbjct: 424 ETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRK 462


>SYK_CHLMU (Q9PLE1) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA
           ligase) (LysRS)
          Length = 524

 Score = 35.0 bits (79), Expect = 0.38
 Identities = 16/39 (41%), Positives = 23/39 (58%)

Query: 237 EDIYERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEK 275
           E + ERF  FCLGK   N +SEL+    + K++ E + K
Sbjct: 424 ETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRK 462


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.319    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,906,220
Number of Sequences: 164201
Number of extensions: 2432084
Number of successful extensions: 5209
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 5154
Number of HSP's gapped (non-prelim): 31
length of query: 436
length of database: 59,974,054
effective HSP length: 113
effective length of query: 323
effective length of database: 41,419,341
effective search space: 13378447143
effective search space used: 13378447143
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)