BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.14 - phase: 0
(159 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRT_PERAM (Q9XZ71) Troponin T (TnT) 40 0.002
HS1_HUMAN (P14317) Hematopoietic lineage cell specific protein (... 38 0.011
GBA1_TRIVA (Q86D96) Guanine nucleotide-binding protein alpha-1 s... 37 0.014
Y055_MYCGE (P47301) Hypothetical protein MG055 37 0.019
YQ38_CAEEL (Q09459) Hypothetical protein C09G5.8 in chromosome II 35 0.055
TONB_NEIGO (O06432) TonB protein 35 0.055
TONB_HELPY (O25899) TonB protein 35 0.055
NOL3_RAT (Q62881) Nucleolar protein 3 35 0.055
CHIT_MANSE (P36362) Endochitinase precursor (EC 3.2.1.14) 35 0.071
YHJ6_YEAST (P38770) Hypothetical 53.4 kDa protein in SLT2-PUT2 i... 33 0.21
STI1_MOUSE (Q60864) Stress-induced-phosphoprotein 1 (STI1) (Hsc7... 33 0.21
SRE2_HUMAN (Q12772) Sterol regulatory element binding protein-2 ... 33 0.21
SECE_THEMA (P35874) Preprotein translocase secE subunit 33 0.21
FTK1_RALSO (Q8XRH0) DNA translocase ftsK 1 33 0.21
TONB_KLEPN (P45610) TonB protein 33 0.27
TONB_ECOLI (P02929) TonB protein 33 0.27
SAH1_HUMAN (O94885) SAM and SH3 domains containing protein 1 (Pr... 33 0.27
NOL3_MOUSE (Q9D1X0) Nucleolar protein 3 33 0.27
TRA1_MAIZE (P08770) Putative AC transposase (ORFA) 33 0.35
TONB_SALTY (P25945) TonB protein 33 0.35
>TRT_PERAM (Q9XZ71) Troponin T (TnT)
Length = 384
Score = 40.4 bits (93), Expect = 0.002
Identities = 17/40 (42%), Positives = 26/40 (64%)
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
+E ++PT PEPEPEP+ E E E E ++ +E +E+E
Sbjct: 343 DELEEPTFEPEPEPEPEEEAAEEEAEEEEEEEEEEEEEEE 382
Score = 29.6 bits (65), Expect = 3.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Query: 57 DQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
D P EPEP+PE E E ++ +E +E+E
Sbjct: 342 DDELEEPTFEPEPEPEPEEEAAEEEAEEEEEEEEEEE 378
>HS1_HUMAN (P14317) Hematopoietic lineage cell specific protein
(Hematopoietic cell-specific LYN substrate 1) (LckBP1)
Length = 486
Score = 37.7 bits (86), Expect = 0.011
Identities = 37/107 (34%), Positives = 52/107 (48%), Gaps = 16/107 (14%)
Query: 36 PSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPE-LNESELASELKKAMQERKEQEG 94
P+LP TL VEE+ + PEPEPEP+PE N+ E E+ + QE E EG
Sbjct: 342 PALPPRTLEGL-----QVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQE-DEPEG 395
Query: 95 NNFWNGVVSEIGEIEWPEFGKVL-GTTGVVLSVIFGSSAVLLTLNAI 140
+ E+ E E F L G++G G+ AV L ++A+
Sbjct: 396 D------YEEVLEPEDSSFSSALAGSSGCPAGA--GAGAVALGISAV 434
>GBA1_TRIVA (Q86D96) Guanine nucleotide-binding protein alpha-1
subunit (G alpha 1) (GTP-binding protein alpha subunit
1)
Length = 402
Score = 37.4 bits (85), Expect = 0.014
Identities = 15/38 (39%), Positives = 23/38 (60%)
Query: 35 KPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPE 72
KPS PS +P+ VE+ +P P+P+PEP+P+
Sbjct: 7 KPSEPSNAKLPSAPVPKKVEQVPEPKPEPQPQPEPQPQ 44
>Y055_MYCGE (P47301) Hypothetical protein MG055
Length = 123
Score = 37.0 bits (84), Expect = 0.019
Identities = 31/115 (26%), Positives = 55/115 (46%), Gaps = 10/115 (8%)
Query: 30 FNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQER 89
F+FK K L + L + ++ + + + + E + E +ES LA E K + +
Sbjct: 7 FSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHES-LAKEKKPKLPFK 65
Query: 90 KEQEGNNFWNGVVSEIGEIEWPE-------FGKVLGTTGVVLSVIFGSSAVLLTL 137
K N W GV EI +I W + F +L +G+++ + FG + +L+TL
Sbjct: 66 KRIV--NLWFGVDKEINKIVWVKGRQLIIIFLLILLVSGLMVGIFFGINQLLITL 118
>YQ38_CAEEL (Q09459) Hypothetical protein C09G5.8 in chromosome II
Length = 1531
Score = 35.4 bits (80), Expect = 0.055
Identities = 33/121 (27%), Positives = 49/121 (40%), Gaps = 12/121 (9%)
Query: 14 SPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPEL 73
SP TP P L P N + + + + V+ + +P S PEPEP + E
Sbjct: 1183 SPEDTPRPSDPLKPNGTNESKESTPVTQRSVDKTDDVAPVDPELEPESGPEPEPVVESEP 1242
Query: 74 NESELASELKKAMQERKEQEGNNFWNGV----------VSEIGEIEWPEFGKVLGTTGVV 123
NE E +K +E K +E + + V I E PE + G+TG +
Sbjct: 1243 NEVAETEEDRK--RELKTEELKSLLGALPPIAKPRNIPVGPIALTEQPEATRQQGSTGRI 1300
Query: 124 L 124
L
Sbjct: 1301 L 1301
>TONB_NEIGO (O06432) TonB protein
Length = 283
Score = 35.4 bits (80), Expect = 0.055
Identities = 27/107 (25%), Positives = 41/107 (38%), Gaps = 11/107 (10%)
Query: 15 PHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELN 74
P P PP + P KP L + + +EK +P P+PEPEP+ +
Sbjct: 67 PQPAPDPPKPVEPP------KPVLKPAVTKKADADIQQPKEKPKPEEKPKPEPEPEAKPA 120
Query: 75 ESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVLGTTG 121
+K ++ E GN ++ G + GK GT G
Sbjct: 121 PKPAEKPAEKPSEKPAEHSGN-----ASAKAGSEQGNGEGKGTGTKG 162
>TONB_HELPY (O25899) TonB protein
Length = 285
Score = 35.4 bits (80), Expect = 0.055
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Query: 35 KPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQ 92
K P +T+ P+ + K +P PEP+PEPKPE E+KK +E KE+
Sbjct: 87 KKEEPKKEVTKPKPKPKP-KPKPKPKPKPEPKPEPKPEPKPEPKVEEVKK--EEPKEE 141
Score = 34.3 bits (77), Expect = 0.12
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 56 KDQPTSNPEPEPEPKPELNESELASELKKAMQERKE 91
K +P PEP+PEPKPE E+ E K +++E
Sbjct: 111 KPKPEPKPEPKPEPKPEPKVEEVKKEEPKEEPKKEE 146
Score = 30.4 bits (67), Expect = 1.8
Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
Query: 56 KDQPTSNPEPEPEPKP---ELNESELASELKK--AMQERKEQ 92
K +P P+PEP+P+P E+ + E E KK A +E KE+
Sbjct: 113 KPEPKPEPKPEPKPEPKVEEVKKEEPKEEPKKEEAKEEAKEK 154
Score = 28.5 bits (62), Expect = 6.7
Identities = 20/71 (28%), Positives = 28/71 (39%), Gaps = 20/71 (28%)
Query: 54 EEKDQPTSNPEPEP--------EPKPELNESELASELKK------------AMQERKEQE 93
E K +P P+PEP EPK E + E E K+ ++ K++E
Sbjct: 115 EPKPEPKPEPKPEPKVEEVKKEEPKEEPKKEEAKEEAKEKSAPKQVTTKDIVKEKDKQEE 174
Query: 94 GNNFWNGVVSE 104
N G SE
Sbjct: 175 SNKTSEGATSE 185
>NOL3_RAT (Q62881) Nucleolar protein 3
Length = 221
Score = 35.4 bits (80), Expect = 0.055
Identities = 19/42 (45%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
Query: 53 VEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQEG 94
VE + +P PEPEPEP+P E E E + QE E EG
Sbjct: 179 VEPEPEPEPEPEPEPEPEP---EPEPEPEREPDFQEGDESEG 217
>CHIT_MANSE (P36362) Endochitinase precursor (EC 3.2.1.14)
Length = 554
Score = 35.0 bits (79), Expect = 0.071
Identities = 21/64 (32%), Positives = 30/64 (46%), Gaps = 6/64 (9%)
Query: 15 PHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRA------VEEKDQPTSNPEPEPE 68
P TPS PS P KP+ + T + T A ++E++ PEP+PE
Sbjct: 417 PPSTPSDPSEGDPIPTTTTAKPASTTKTTVKTTTTTTAKPPQSVIDEENDINVRPEPKPE 476
Query: 69 PKPE 72
P+PE
Sbjct: 477 PQPE 480
>YHJ6_YEAST (P38770) Hypothetical 53.4 kDa protein in SLT2-PUT2
intergenic region
Length = 471
Score = 33.5 bits (75), Expect = 0.21
Identities = 35/110 (31%), Positives = 48/110 (42%), Gaps = 17/110 (15%)
Query: 24 TLSPQFFNFKLKPSLPSLTLTRRCTLP----RAVEEKDQ--------PTSNPEP---EP- 67
TLSPQ N + P PS R TL +A EE+D TS E EP
Sbjct: 38 TLSPQLIN-RFMPHFPSSPSPLRNTLDFSAAKADEEEDDRMEIDEVDDTSFEEEYNNEPI 96
Query: 68 EPKPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVL 117
E E E+ + E++ +ER++QE N + V E+ I P V+
Sbjct: 97 ETHTEATENAVVEEIEATPEERQKQEKNESQDQSVEEVENIVSPHRSTVI 146
>STI1_MOUSE (Q60864) Stress-induced-phosphoprotein 1 (STI1)
(Hsc70/Hsp90-organizing protein) (Hop) (mSTI1)
Length = 543
Score = 33.5 bits (75), Expect = 0.21
Identities = 15/37 (40%), Positives = 22/37 (58%)
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASELKKAMQERK 90
EE P P P+ EPKPE E +L K+A++E++
Sbjct: 194 EEAATPPPPPPPKKEPKPEPMEEDLPENKKQALKEKE 230
>SRE2_HUMAN (Q12772) Sterol regulatory element binding protein-2
(SREBP-2) (Sterol regulatory element-binding
transcription factor 2)
Length = 1141
Score = 33.5 bits (75), Expect = 0.21
Identities = 22/65 (33%), Positives = 30/65 (45%), Gaps = 13/65 (20%)
Query: 8 SFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEP 67
SFT P F+PS SPQ ++K S S+ T R T P P P+P
Sbjct: 91 SFTQVTLPSFSPS---AASPQAPTLQVKVSPTSVPTTPRAT----------PILQPRPQP 137
Query: 68 EPKPE 72
+P+P+
Sbjct: 138 QPQPQ 142
>SECE_THEMA (P35874) Preprotein translocase secE subunit
Length = 65
Score = 33.5 bits (75), Expect = 0.21
Identities = 16/58 (27%), Positives = 31/58 (52%)
Query: 97 FWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIFGSSAVLLTLNAILAELSDKVFAGKGV 154
F+ V++E +I WP ++L + GVVL ++ +S L+ I + + +F G+
Sbjct: 7 FFREVIAEAKKISWPSRKELLTSFGVVLVILAVTSVYFFVLDFIFSGVVSAIFKALGI 64
>FTK1_RALSO (Q8XRH0) DNA translocase ftsK 1
Length = 959
Score = 33.5 bits (75), Expect = 0.21
Identities = 27/94 (28%), Positives = 41/94 (42%), Gaps = 22/94 (23%)
Query: 13 LSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLT-------------RRCTLPRAVEEKDQP 59
+SP P P++ P+ +PS P++ L R P A P
Sbjct: 276 ISPAAEPDAPASAPPE----PAEPSPPTVDLEAVRQEAEALLAELRGLMTPLAAA----P 327
Query: 60 TSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
++PEPEPEP+PE E+E+ E + + E E
Sbjct: 328 VASPEPEPEPEPEA-ETEVTPEAEAEPEAEPEAE 360
>TONB_KLEPN (P45610) TonB protein
Length = 243
Score = 33.1 bits (74), Expect = 0.27
Identities = 17/46 (36%), Positives = 24/46 (51%), Gaps = 2/46 (4%)
Query: 53 VEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQ--EGNN 96
V K +P P+P+P+PKPE + E+K A + R E NN
Sbjct: 88 VIHKPEPKPKPKPKPKPKPEKKVEQPKREVKPAAEPRPASPFENNN 133
>TONB_ECOLI (P02929) TonB protein
Length = 239
Score = 33.1 bits (74), Expect = 0.27
Identities = 13/23 (56%), Positives = 16/23 (69%)
Query: 50 PRAVEEKDQPTSNPEPEPEPKPE 72
P+AV+ +P PEPEPEP PE
Sbjct: 58 PQAVQPPPEPVVEPEPEPEPIPE 80
>SAH1_HUMAN (O94885) SAM and SH3 domains containing protein 1
(Proline-glutamate repeat-containing protein)
Length = 1247
Score = 33.1 bits (74), Expect = 0.27
Identities = 15/29 (51%), Positives = 17/29 (57%)
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASEL 82
E + +P PEPEPEPKP SE S L
Sbjct: 16 EPEPEPEPAPEPEPEPKPGAGTSEAFSRL 44
Score = 28.1 bits (61), Expect = 8.7
Identities = 15/41 (36%), Positives = 19/41 (45%)
Query: 55 EKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQEGN 95
E+D PT EP P+ PE+ + AS K ER N
Sbjct: 850 EQDVPTEVTEPPPQIVPEVPQKTTASSTKAQPLERDSAVDN 890
Score = 28.1 bits (61), Expect = 8.7
Identities = 10/19 (52%), Positives = 13/19 (67%)
Query: 54 EEKDQPTSNPEPEPEPKPE 72
E + +P PEP PEP+PE
Sbjct: 12 EPEPEPEPEPEPAPEPEPE 30
>NOL3_MOUSE (Q9D1X0) Nucleolar protein 3
Length = 220
Score = 33.1 bits (74), Expect = 0.27
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 5/45 (11%)
Query: 54 EEKDQPTSNPEPEPEPKPELN-----ESELASELKKAMQERKEQE 93
E++ +P PEPEPEP+PE E E E + QE E E
Sbjct: 174 EQEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPDFQEEDESE 218
Score = 30.8 bits (68), Expect = 1.3
Identities = 11/20 (55%), Positives = 15/20 (75%)
Query: 53 VEEKDQPTSNPEPEPEPKPE 72
+E++ P PEPEPEP+PE
Sbjct: 167 LEQEMNPEQEPEPEPEPEPE 186
>TRA1_MAIZE (P08770) Putative AC transposase (ORFA)
Length = 806
Score = 32.7 bits (73), Expect = 0.35
Identities = 22/92 (23%), Positives = 42/92 (44%), Gaps = 6/92 (6%)
Query: 18 TPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESE 77
+PS + + + F + S + T T A+ + QP P+PEP+P+P+ E
Sbjct: 69 SPSSSTAEAERHFIQSVSSSNANGTATDPSQDDMAIVHEPQPQPQPQPEPQPQPQPEPEE 128
Query: 78 LASELKKAMQERKEQEGNNFWNGVVSEIGEIE 109
+A Q+R ++ ++ W + E+E
Sbjct: 129 ------EAPQKRAKKCTSDVWQHFTKKEIEVE 154
>TONB_SALTY (P25945) TonB protein
Length = 242
Score = 32.7 bits (73), Expect = 0.35
Identities = 12/22 (54%), Positives = 16/22 (72%)
Query: 50 PRAVEEKDQPTSNPEPEPEPKP 71
P+AV+ +P PEPEPEP+P
Sbjct: 58 PQAVQPPPEPVVEPEPEPEPEP 79
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.312 0.132 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,477,152
Number of Sequences: 164201
Number of extensions: 864046
Number of successful extensions: 5231
Number of sequences better than 10.0: 175
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 87
Number of HSP's that attempted gapping in prelim test: 4443
Number of HSP's gapped (non-prelim): 503
length of query: 159
length of database: 59,974,054
effective HSP length: 101
effective length of query: 58
effective length of database: 43,389,753
effective search space: 2516605674
effective search space used: 2516605674
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC136286.14