BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135799.6 + phase: 0
(321 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TL21_CHICK (Q9DD78) Toll-like receptor 2 type 1 precursor 35 0.25
TL22_CHICK (Q9DGB6) Toll-like receptor 2 type 2 precursor 33 0.73
FXLL_MOUSE (Q8BFZ4) F-box/LRR-repeat protein 21 (F-box and leuci... 33 0.95
YMR9_YEAST (Q05040) Hypothetical 59.3 kDa protein in TAP42-CYK2 ... 32 1.6
EX70_SCHPO (Q10339) 70 kDa exocyst complex protein 32 2.8
ARLY_PASMU (P57909) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 31 4.7
RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC... 30 6.2
PAC2_YEAST (P39937) PAC2 protein 30 6.2
Y367_RICPR (Q9ZDG2) Hypothetical protein RP367 30 8.1
SEN1_YEAST (Q00416) Helicase SEN1 (EC 3.6.1.-) (tRNA-splicing en... 30 8.1
RM10_YEAST (P36520) 60S ribosomal protein L10, mitochondrial pre... 30 8.1
A8B1_HUMAN (O43520) Potential phospholipid-transporting ATPase I... 30 8.1
>TL21_CHICK (Q9DD78) Toll-like receptor 2 type 1 precursor
Length = 793
Score = 35.0 bits (79), Expect = 0.25
Identities = 43/177 (24%), Positives = 75/177 (42%), Gaps = 25/177 (14%)
Query: 108 IKNIHKWVDIVVKSKVQNIHLYPHV---PLNGEETILPKFPMSSILSC--TTLVVLKLRW 162
I N+HK +++ + N P + P N + L + + +C +TL VL +
Sbjct: 418 ISNLHKLINLDISEN--NFGEIPDMCEWPENLKYLNLSSTQIPKLTTCIPSTLEVLDVSA 475
Query: 163 FNLTVVSDLSIRLPSLKTLHLKEIYFDQQRDFMMLLDGCPVLEDLQLCYIYMTRQSHHSL 222
NL D ++LP LK L+L ++ + L + +L I + + S
Sbjct: 476 NNL---QDFGLQLPFLKELYLT-------KNHLKTLPEATDIPNLVAMSISRNKLNSFSK 525
Query: 223 DDFESSSMLKKLNR-ADITDCECYFPVKSLSNLEFLRIKLSEVCFIIEWNESEQCDS 278
++FES ++ L+ A+ C C F L F+ + ++ W ES CDS
Sbjct: 526 EEFESFKQMELLDASANNFICSCEF-------LSFIHHEAGIAQVLVGWPESYICDS 575
>TL22_CHICK (Q9DGB6) Toll-like receptor 2 type 2 precursor
Length = 781
Score = 33.5 bits (75), Expect = 0.73
Identities = 39/148 (26%), Positives = 64/148 (42%), Gaps = 25/148 (16%)
Query: 134 LNGEETILPKFPMSSILSC--TTLVVLKLRWFNLTVVSDLSIRLPSLKTLHLKEIYFDQQ 191
LN T +PK + +C +TL VL + NL D ++LP LK L+L
Sbjct: 438 LNLSSTQIPK-----LTTCIPSTLEVLDVSANNL---QDFGLQLPFLKELYLT------- 482
Query: 192 RDFMMLLDGCPVLEDLQLCYIYMTRQSHHSLDDFESSSMLKKLNR-ADITDCECYFPVKS 250
++ + L + +L I + + S ++FES ++ L+ A+ C C F
Sbjct: 483 KNHLKTLPEATDIPNLVAMSISRNKLNSFSKEEFESFKQMELLDASANNFICSCEF---- 538
Query: 251 LSNLEFLRIKLSEVCFIIEWNESEQCDS 278
L F+ + ++ W ES CDS
Sbjct: 539 ---LSFIHHEAGIAQVLVGWPESYICDS 563
>FXLL_MOUSE (Q8BFZ4) F-box/LRR-repeat protein 21 (F-box and
leucine-rich repeat protein 21) (F-box/LRR-repeat
protein 3B) (F-box and leucine-rich repeat protein 3B)
Length = 434
Score = 33.1 bits (74), Expect = 0.95
Identities = 39/185 (21%), Positives = 79/185 (42%), Gaps = 23/185 (12%)
Query: 3 KQKISTSVIDRISSFPDDILIRILSSLPIEQACVTSILSKRWTHLWCFVPDLDFTKTKWK 62
+Q + SV+ + P ++++I LP+ S + +RW ++ +PDL + K +++
Sbjct: 31 RQTHALSVLLDWGTLPHHVILQIFQYLPLIDRARASSVCRRWNEVF-HIPDL-WRKFEFE 88
Query: 63 DQESYSRFEEFVFSVLYS---REAAGNHSINTFILDIEYDSSELLLDGIKNIHKWVDIVV 119
+S + + + L ++ A + +F +D +S+E D + + V
Sbjct: 89 LNQSATSYFKSTHPDLIQQIIKKHAAHLQYVSFKVDSSTESAEAACDILSQL-------V 141
Query: 120 KSKVQNIHLYPHVPLNGEETILPKF---PMSSILSCTTLVVLKLRWFNLTVVSDLSIRLP 176
+Q + L T P F P S +S T+V + + + + D + P
Sbjct: 142 NCSIQTLGLI--------STAKPSFMNVPKSHFVSALTVVFVNSKSLSSIKIEDTPVDDP 193
Query: 177 SLKTL 181
SLK L
Sbjct: 194 SLKIL 198
>YMR9_YEAST (Q05040) Hypothetical 59.3 kDa protein in TAP42-CYK2
intergenic region
Length = 523
Score = 32.3 bits (72), Expect = 1.6
Identities = 39/148 (26%), Positives = 61/148 (40%), Gaps = 11/148 (7%)
Query: 1 MSKQKISTSVIDRISSFPDDILIRILSSLPIEQACVTSILSKRWTHLWCFVPDLDFTKTK 60
+SK S +D ISS+ D + + + +E V + L+ + D D K
Sbjct: 187 ISKIHSELSKVDIISSYGDCMALYDADTKSLEIHQVDANLNSKLLKKISLGQDSDIMKFF 246
Query: 61 WKDQESYSRFEEFVFSVLYSREAAGNHSINTFILDIE--YDSSELLLDGIKNIHKWVDIV 118
W E+ L+S +A S + D E + SS+++ KN KW+ I
Sbjct: 247 WVSTSKLLVIEKSFHLKLFSISSASLISDVDLLQDSEQPFSSSDIINIDFKN--KWLLIA 304
Query: 119 VKSKVQ-------NIHLYPHVPLNGEET 139
K+K Q NI VP+N +ET
Sbjct: 305 SKNKSQIRIWELDNIEAPEDVPINIKET 332
>EX70_SCHPO (Q10339) 70 kDa exocyst complex protein
Length = 615
Score = 31.6 bits (70), Expect = 2.8
Identities = 27/115 (23%), Positives = 49/115 (42%), Gaps = 14/115 (12%)
Query: 165 LTVVSDLSIRLPSLKTLHLKEIYFDQQRDFMMLLDGCPVLEDLQLCYIYMTRQSHHSLD- 223
+ ++ L+ SL+ L+ QQ+ MML +GC +L LCY + + SL+
Sbjct: 107 MDILKSLAEDYTSLEGSPLQFASKSQQKVEMMLSEGCQILG--ALCYNILETYAASSLNK 164
Query: 224 -----------DFESSSMLKKLNRADITDCECYFPVKSLSNLEFLRIKLSEVCFI 267
F + S+ + + D + FPV S++ + IK+ C +
Sbjct: 165 ASTLLDLSIPWSFPNESLQQFIGLIQQFDADVLFPVSCSSDISNIYIKIKGECVV 219
>ARLY_PASMU (P57909) Argininosuccinate lyase (EC 4.3.2.1)
(Arginosuccinase) (ASAL)
Length = 457
Score = 30.8 bits (68), Expect = 4.7
Identities = 11/39 (28%), Positives = 24/39 (61%)
Query: 88 SINTFILDIEYDSSELLLDGIKNIHKWVDIVVKSKVQNI 126
++NT ++++ + +L D ++IH WV+ + KV N+
Sbjct: 61 ALNTLLIEVRSNPQAILQDDAEDIHSWVESKLIDKVGNL 99
>RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1332
Score = 30.4 bits (67), Expect = 6.2
Identities = 12/42 (28%), Positives = 23/42 (54%)
Query: 249 KSLSNLEFLRIKLSEVCFIIEWNESEQCDSFVFLIFNFIELN 290
KS+ + I+L C ++ WNE E+ S + +F+E++
Sbjct: 794 KSIRGILDTSIQLVRTCLVLNWNEDEKSSSIEEALASFVEVS 835
>PAC2_YEAST (P39937) PAC2 protein
Length = 518
Score = 30.4 bits (67), Expect = 6.2
Identities = 25/113 (22%), Positives = 51/113 (45%), Gaps = 12/113 (10%)
Query: 93 ILDIEYDSSELLLDGIKNIHKWVDIVVKS---KVQNIHLYPHVP-------LNGEETILP 142
+LD+ Y++ L GI+N + ++ N+ +P P LN +
Sbjct: 232 MLDLSYNN--LTSAGIQNFENEIPCTLEELNISGNNLISFPLFPKNLTLKGLNVSNNQIS 289
Query: 143 KFPMSSILSCTTLVVLKLRWFNLTVVSDLSIRLPSLKTLHLKEIYFDQQRDFM 195
+ P +I S +L + ++ +++ DL+ PSLK +HL F+ +++
Sbjct: 290 RAPSIAIYSVESLDITDNKFKERSLIDDLNKTFPSLKNIHLSGNEFNYNGNYI 342
>Y367_RICPR (Q9ZDG2) Hypothetical protein RP367
Length = 303
Score = 30.0 bits (66), Expect = 8.1
Identities = 19/68 (27%), Positives = 35/68 (50%), Gaps = 2/68 (2%)
Query: 67 YSRFEEFVFSVLYSREAAGNHSINTFILDIEYDSSELLLDGIKNIHKWVDIVVKSKVQNI 126
+ +F F+F +L + + I+ +E+DS ++ GI+ K DI V+ V+N
Sbjct: 7 FQKFFSFLFVLLITTLVCADPKKVAVIVPLEHDSMYKIVSGIQEALK--DIDVEILVKNA 64
Query: 127 HLYPHVPL 134
H P++ L
Sbjct: 65 HADPNILL 72
>SEN1_YEAST (Q00416) Helicase SEN1 (EC 3.6.1.-) (tRNA-splicing
endonuclease positive effector)
Length = 2231
Score = 30.0 bits (66), Expect = 8.1
Identities = 22/102 (21%), Positives = 40/102 (38%), Gaps = 9/102 (8%)
Query: 10 VIDRISSFPDDILIRILSSLPIEQACVTSILSKRWTHLWCFVPDLDFTKTKWKDQESYSR 69
++D +S+F D I + ++ K W W F+ + KW + YS
Sbjct: 739 LMDVVSAFVDPISGVFANFQTLKSQNTEKEFLKFWESCWLFLDTIYKFTLKWASKYDYSE 798
Query: 70 FEEFVFSVLYSREAAGNHSINTFILDIEYDSSELLLDGIKNI 111
E F L ++ ++D + S++L D KN+
Sbjct: 799 LENFTKDTL---------DLSRSLVDSFREFSDILHDQTKNL 831
>RM10_YEAST (P36520) 60S ribosomal protein L10, mitochondrial
precursor (YmL10/YmL18)
Length = 322
Score = 30.0 bits (66), Expect = 8.1
Identities = 22/80 (27%), Positives = 37/80 (45%), Gaps = 7/80 (8%)
Query: 59 TKWKDQESYSRFEEFVFSVLYS-------REAAGNHSINTFILDIEYDSSELLLDGIKNI 111
TK +D + YS+ E+ + V+ +EA S + +++ S E+ L+ +
Sbjct: 243 TKRRDIDFYSKEEKRGYLVMEKDKLLQDIKEAQNKGSRHFLKQNVKKSSLEIELEELSPE 302
Query: 112 HKWVDIVVKSKVQNIHLYPH 131
WV +V SKV NI H
Sbjct: 303 KDWVPVVSNSKVMNIKALDH 322
>A8B1_HUMAN (O43520) Potential phospholipid-transporting ATPase IC (EC
3.6.3.1) (Familial intrahepatic cholestasis type 1)
(ATPase class I type 8B member 1)
Length = 1251
Score = 30.0 bits (66), Expect = 8.1
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 2/60 (3%)
Query: 153 TTLVVLKLRWFNLTVVSDLSIRLPSLKTLHLKEIYFDQQRDFMMLLDGCPVLEDLQLCYI 212
T+L VL + + V LS+R P L + +++ F+ +R F+ LL G VL + L +I
Sbjct: 993 TSLPVLLMGLLDQDVSDKLSLRFPGLYIVGQRDLLFNYKRFFVSLLHG--VLTSMILFFI 1050
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.139 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,984,732
Number of Sequences: 164201
Number of extensions: 1398769
Number of successful extensions: 3758
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 3754
Number of HSP's gapped (non-prelim): 14
length of query: 321
length of database: 59,974,054
effective HSP length: 110
effective length of query: 211
effective length of database: 41,911,944
effective search space: 8843420184
effective search space used: 8843420184
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 66 (30.0 bits)
Medicago: description of AC135799.6