BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135799.5 + phase: 0
(171 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RFA1_YEAST (P22336) Replication factor-A protein 1 (RF-A) (Singl... 34 0.19
2ACA_HUMAN (Q06190) Serine/threonine protein phosphatase 2A, 72/... 33 0.25
ASPM_MACFA (P62291) Abnormal spindle-like microcephaly-associate... 33 0.42
ASPM_MACMU (P62292) Abnormal spindle-like microcephaly-associate... 32 0.55
ASPM_COLGU (P62287) Abnormal spindle-like microcephaly-associate... 32 0.72
ASPM_PONPY (P62294) Abnormal spindle-like microcephaly-associate... 31 1.2
ASPM_PANTR (P62293) Abnormal spindle-like microcephaly-associate... 31 1.2
ASPM_HYLLA (P62290) Abnormal spindle-like microcephaly-associate... 31 1.2
ASPM_HUMAN (Q8IZT6) Abnormal spindle-like microcephaly-associate... 31 1.2
ASPM_GORGO (P62289) Abnormal spindle-like microcephaly-associate... 31 1.2
ASPM_CANFA (P62286) Abnormal spindle-like microcephaly-associate... 30 2.7
DIP2_YEAST (Q12220) DOM34 interacting protein 2 30 3.5
XYLH_ECOLI (P37389) Xylose transport system permease protein xylH 29 4.6
RFA1_CRIFA (Q23696) Replication factor A 51 kDa subunit (RP-A) (... 29 6.1
QACR_STAAM (P23217) HTH-type transcriptional regulator qacR 29 6.1
CRB2_BOVIN (P02522) Beta crystallin B2 (BP) 29 6.1
>RFA1_YEAST (P22336) Replication factor-A protein 1 (RF-A)
(Single-stranded DNA-binding protein) (DNA binding
protein BUF2) (Replication protein A 69 kDa DNA-binding
subunit)
Length = 621
Score = 33.9 bits (76), Expect = 0.19
Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 2/95 (2%)
Query: 49 EKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLSMMHNSTFT 108
+ G I AT D KF ++QEG Y + A + + + +H ++L++ ++
Sbjct: 228 DTSGEIRATAFNDFATKFNEILQEGKVYYVSKAKLQPAKPQFTNLTHPYELNLDRDTVIE 287
Query: 109 KVHLPA-IPMNVFEFKPFNEILSSTVEEVSTDVIG 142
+ + +P F F + I + V + DV+G
Sbjct: 288 ECFDESNVPKTHFNFIKLDAIQNQEVNS-NVDVLG 321
>2ACA_HUMAN (Q06190) Serine/threonine protein phosphatase 2A, 72/130
kDa regulatory subunit B (PP2A, subunit B,
B''-PR72/PR130) (PP2A, subunit B, B72/B130 isoforms)
(PP2A, subunit B, PR72/PR130 isoforms) (PP2A, subunit B,
R3 isoform)
Length = 1150
Score = 33.5 bits (75), Expect = 0.25
Identities = 28/116 (24%), Positives = 51/116 (43%), Gaps = 7/116 (6%)
Query: 60 KDLVAKF-----RSMVQEGGTYQLENAIVDFNESPYKV-TSHKHKLSMMHNSTFTKVHLP 113
K V+KF R +++ ++D K+ TS + L+ NS F H
Sbjct: 486 KSKVSKFEEGDQRDFTNSSSQEEIDKLLMDLESFSQKMETSLREPLAKGKNSNFLNSHSQ 545
Query: 114 AIPMNVFEFKPFNEILSSTVEEVSTDVIGHVIERGDIRETEKDRRKSRVIDLTLED 169
+ + +P +++ SS +E+VS + +IE + E+DR I ++ED
Sbjct: 546 LTGQTLVDLEPKSKV-SSPIEKVSPSCLTRIIETNGHKIEEEDRALLLRILESIED 600
>ASPM_MACFA (P62291) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3476
Score = 32.7 bits (73), Expect = 0.42
Identities = 28/101 (27%), Positives = 47/101 (45%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS---MMH 103
V +KGG I T L+A + + +VD S YK+T+HKHK++ ++H
Sbjct: 3360 VADKGGSIFTKTCC-LLAVLLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILH 3418
Query: 104 ----NSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3419 KQKKNSSISIPFIPXTPVRTRIVSRLKPDWVLRRDNMEEIT 3459
>ASPM_MACMU (P62292) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3479
Score = 32.3 bits (72), Expect = 0.55
Identities = 28/101 (27%), Positives = 47/101 (45%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS---MMH 103
V +KGG I T L+A + + +VD S YK+T+HKHK++ ++H
Sbjct: 3363 VADKGGSIFTKTCC-LLAVLLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILH 3421
Query: 104 ----NSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3422 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3462
>ASPM_COLGU (P62287) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 32.0 bits (71), Expect = 0.72
Identities = 28/101 (27%), Positives = 47/101 (45%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS---MMH 103
V +KGG I T L+A + + +VD S YK+T+HKHK++ ++H
Sbjct: 3361 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILH 3419
Query: 104 ----NSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3420 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3460
>ASPM_PONPY (P62294) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3471
Score = 31.2 bits (69), Expect = 1.2
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + +VD S YK+T+HKHK++
Sbjct: 3355 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILY 3413
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3414 KQKKNSSISIPFIPETPVRTRIVSRLKPNWVLRRDNMEEIT 3454
>ASPM_PANTR (P62293) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 31.2 bits (69), Expect = 1.2
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + +VD S YK+T+HKHK++
Sbjct: 3361 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILY 3419
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3420 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3460
>ASPM_HYLLA (P62290) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 31.2 bits (69), Expect = 1.2
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + +VD S YK+T+HKHK++
Sbjct: 3361 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILY 3419
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3420 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3460
>ASPM_HUMAN (Q8IZT6) Abnormal spindle-like microcephaly-associated
protein (Abnormal spindle protein homolog) (Asp homolog)
Length = 3477
Score = 31.2 bits (69), Expect = 1.2
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + +VD S YK+T+HKHK++
Sbjct: 3361 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILY 3419
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3420 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3460
>ASPM_GORGO (P62289) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3476
Score = 31.2 bits (69), Expect = 1.2
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + +VD S YK+T+HKHK++
Sbjct: 3360 VADKGGSIFTKTCC-LLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILY 3418
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3419 KQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3459
>ASPM_CANFA (P62286) Abnormal spindle-like microcephaly-associated
protein homolog (Fragment)
Length = 3452
Score = 30.0 bits (66), Expect = 2.7
Identities = 26/101 (25%), Positives = 45/101 (43%), Gaps = 11/101 (10%)
Query: 47 VDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHKHKLS------ 100
V +KGG I T L+A + + ++ +VD S YK+T+ KHK++
Sbjct: 3353 VADKGGSIFTKTCC-LLAVLLKTTNRASSVRSKSKVVDRIYSLYKLTARKHKMNTERTLY 3411
Query: 101 -MMHNSTFTKVHLPAIPMN---VFEFKPFNEILSSTVEEVS 137
NS+ + +P P+ V KP + +EE++
Sbjct: 3412 KQKMNSSISTPFIPETPVRTRIVSRLKPDWVLRRDNMEEIT 3452
>DIP2_YEAST (Q12220) DOM34 interacting protein 2
Length = 943
Score = 29.6 bits (65), Expect = 3.5
Identities = 27/83 (32%), Positives = 40/83 (47%), Gaps = 4/83 (4%)
Query: 77 QLENAIVDFNESPYKVTSHKHKLSMMHNSTFTKVHLPAIPMNVFEFKPFN--EILSSTVE 134
QLE+A++ S Y + K ++M N T HLP I N+F FN E++S E
Sbjct: 816 QLEDALMVMPFS-YVLKFLKFIDTVMQNKTLLHSHLPLICKNLFFIIKFNHKELVSQKNE 874
Query: 135 EVSTDVIGHVIE-RGDIRETEKD 156
E+ + E R ++ TE D
Sbjct: 875 ELKLQINRVKTELRSALKSTEDD 897
>XYLH_ECOLI (P37389) Xylose transport system permease protein xylH
Length = 393
Score = 29.3 bits (64), Expect = 4.6
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
Query: 6 TPISAVSGGRKNLKMCVRVAHIWLIRE-KKVPTSIIFMNMLLVDEKGGRIHAT 57
+P S GR+ L + + IWL+ + + VPT ++ + +LL+ GG AT
Sbjct: 211 SPASTAVVGRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLL---GGMFMAT 260
>RFA1_CRIFA (Q23696) Replication factor A 51 kDa subunit (RP-A)
(RF-A) (Replication factor-A protein 1) (Single-stranded
DNA-binding protein P51 subunit)
Length = 467
Score = 28.9 bits (63), Expect = 6.1
Identities = 35/133 (26%), Positives = 51/133 (38%), Gaps = 7/133 (5%)
Query: 23 RVAHIWLIREKKVPTSI-IFMNMLLVDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENA 81
RVA IR PTS + L+DE I AT D V F +V G Y
Sbjct: 28 RVADKSDIRTWNKPTSQGKLFSFTLIDESAA-IRATVFNDAVDTFEPLVVNGQVYYFSGG 86
Query: 82 IVDFNESPYKVTSHKHKLSMMHNSTFTKVH----LPAIPMNVFEFKPFNEILSSTVEEVS 137
V + ++ ++L+ S A+PM + F P E+L
Sbjct: 87 QVKNANRRFSNVNNDYELTFDRASEVILARQDSSAAALPMQRYNFVPI-ELLKQREVGSL 145
Query: 138 TDVIGHVIERGDI 150
DV+G V++ +I
Sbjct: 146 VDVLGVVLKVDEI 158
>QACR_STAAM (P23217) HTH-type transcriptional regulator qacR
Length = 188
Score = 28.9 bits (63), Expect = 6.1
Identities = 12/37 (32%), Positives = 19/37 (50%)
Query: 76 YQLENAIVDFNESPYKVTSHKHKLSMMHNSTFTKVHL 112
Y L+NAI++F YK S K++ + N H+
Sbjct: 93 YPLQNAIIEFYTEYYKTNSINEKMNKLENKYIDAYHV 129
>CRB2_BOVIN (P02522) Beta crystallin B2 (BP)
Length = 204
Score = 28.9 bits (63), Expect = 6.1
Identities = 20/76 (26%), Positives = 37/76 (48%), Gaps = 11/76 (14%)
Query: 89 PYKVTSHKHKLSMMHNSTFTKVHLPAIPMNV--FEFKPFNEILSSTVEEVSTDV------ 140
P KV S +HK+++ N FT + I +V F + E +SS + T V
Sbjct: 98 PIKVDSQEHKITLYENPNFTGKKMEVIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPG 157
Query: 141 ---IGHVIERGDIRET 153
+ +++E+GD +++
Sbjct: 158 YRGLQYLLEKGDYKDS 173
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.134 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,999,099
Number of Sequences: 164201
Number of extensions: 725710
Number of successful extensions: 1936
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1929
Number of HSP's gapped (non-prelim): 21
length of query: 171
length of database: 59,974,054
effective HSP length: 102
effective length of query: 69
effective length of database: 43,225,552
effective search space: 2982563088
effective search space used: 2982563088
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC135799.5