
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.6 - phase: 0
(267 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
USF2_HUMAN (Q15853) Upstream stimulatory factor 2 (Upstream tran... 40 0.005
USF1_XENBO (Q07957) Upstream stimulatory factor 1 (USF) (SPF1) (... 40 0.006
BMAL_HUMAN (O00327) Aryl hydrocarbon receptor nuclear translocat... 40 0.006
USF1_RABIT (O02818) Upstream stimulatory factor 1 (Major late tr... 40 0.008
USF1_MOUSE (Q61069) Upstream stimulatory factor 1 (Major late tr... 39 0.013
USF1_HUMAN (P22415) Upstream stimulatory factor 1 (Major late tr... 39 0.013
USF2_RAT (Q63665) Upstream stimulatory factor 2 (Upstream transc... 39 0.017
USF2_MOUSE (Q64705) Upstream stimulatory factor 2 (Upstream tran... 39 0.017
RAP1_ARATH (Q39204) Transcription factor AtMYC2 (R-homologous Ar... 39 0.017
BMAL_RAT (Q9EPW1) Aryl hydrocarbon receptor nuclear translocator... 39 0.017
BMAL_MOUSE (Q9WTL8) Aryl hydrocarbon receptor nuclear translocat... 39 0.017
BMAL_MESAU (O88529) Aryl hydrocarbon receptor nuclear translocat... 39 0.017
USO1_YEAST (P25386) Intracellular protein transport protein USO1 38 0.029
HAIR_DROME (P14003) Hairy protein 36 0.085
CYCL_DROME (O61734) Cycle protein (Brain and muscle ARNT-like 1)... 36 0.085
PRE_STRAG (P13925) Plasmid recombination enzyme (Mobilization pr... 36 0.11
CBF1_YEAST (P17106) Centromere-binding protein 1 (CBP-1) (Centro... 36 0.11
CBF1_KLULA (P49379) Centromere-binding protein 1 (CBP-1) (Centro... 35 0.19
NUC1_NEUCR (P20824) Phosphorus acquisition controlling protein 35 0.25
USF_STRPU (Q07956) Upstream stimulatory factor (USF) (SPF1) 34 0.32
>USF2_HUMAN (Q15853) Upstream stimulatory factor 2 (Upstream
transcription factor 2) (FOS-interacting protein) (FIP)
(Major late transcription factor 2)
Length = 346
Score = 40.4 bits (93), Expect = 0.005
Identities = 36/125 (28%), Positives = 59/125 (46%), Gaps = 18/125 (14%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-CNSKTRQGFTS 113
HP+ +I T + +D A H E E+RRR++IN+ + L ++P CN+ +
Sbjct: 219 HPYS-PKIDGTRTPRDERRRA--QHNEVERRRRDKINNWIVQLSKIIPDCNA------DN 269
Query: 114 SKSSRTCERFKSKGSRLAKVVERVKELRQ--QTCQITQVETVPCEADGXTVISAGSDMSG 171
SK+ SKG L+K + ++ELRQ Q Q T E + D + ++
Sbjct: 270 SKTG------ASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKN 323
Query: 172 EGRLI 176
E L+
Sbjct: 324 ENALL 328
>USF1_XENBO (Q07957) Upstream stimulatory factor 1 (USF) (SPF1) (B1
factor)
Length = 307
Score = 40.0 bits (92), Expect = 0.006
Identities = 26/95 (27%), Positives = 49/95 (51%), Gaps = 14/95 (14%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSS 114
HP+ ++ D+ A H E E+RRR++IN+ + L ++P S
Sbjct: 180 HPYSPKSDGPRTTRDDKRRA---QHNEVERRRRDKINNWIVQLSKIIP--------DCSM 228
Query: 115 KSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQ 149
+S++T + SKG L+K + ++ELRQ ++++
Sbjct: 229 ESTKTGQ---SKGGILSKACDYIQELRQSNLRLSE 260
>BMAL_HUMAN (O00327) Aryl hydrocarbon receptor nuclear
translocator-like protein 1 (Brain and muscle ARNT-like
1) (Member of PAS protein 3) (Basic-helix-loop-helix-PAS
orphan MOP3) (bHLH-PAS protein JAP3)
Length = 626
Score = 40.0 bits (92), Expect = 0.006
Identities = 36/121 (29%), Positives = 55/121 (44%), Gaps = 24/121 (19%)
Query: 35 SSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHL 94
SST Q+S + D+ P E T+ Q R A + H + EKRRR+++NS +
Sbjct: 42 SSTDYQESMDTDKDD------PHGRLEYTE---HQGRIKNAREAHSQIEKRRRDKMNSFI 92
Query: 95 DHLRTLLP-CNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQVETV 153
D L +L+P CN+ +R+ K + L V+ +K LR T T+
Sbjct: 93 DELASLVPTCNAMSRK--------------LDKLTVLRMAVQHMKTLRGATNPYTEANYK 138
Query: 154 P 154
P
Sbjct: 139 P 139
>USF1_RABIT (O02818) Upstream stimulatory factor 1 (Major late
transcription factor 1)
Length = 310
Score = 39.7 bits (91), Expect = 0.008
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 14/95 (14%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSS 114
HP+ ++ ++ A H E E+RRR++IN+ + L ++P S S+
Sbjct: 183 HPYSPKSAAPRTTRDEKRRA---QHNEVERRRRDKINNWIVQLSKIIPDCS-----MEST 234
Query: 115 KSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQ 149
KS +SKG L+K + ++ELRQ ++++
Sbjct: 235 KSG------QSKGGILSKACDYIQELRQSNHRLSE 263
>USF1_MOUSE (Q61069) Upstream stimulatory factor 1 (Major late
transcription factor 1)
Length = 310
Score = 38.9 bits (89), Expect = 0.013
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 14/95 (14%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSS 114
HP+ ++ ++ A H E E+RRR++IN+ + L ++P S S+
Sbjct: 183 HPYSPKSEAPRTTRDEKRRA---QHNEVERRRRDKINNWIVQLSKIIPDCS-----MEST 234
Query: 115 KSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQ 149
KS +SKG L+K + ++ELRQ ++++
Sbjct: 235 KSG------QSKGGILSKACDYIQELRQSNHRLSE 263
>USF1_HUMAN (P22415) Upstream stimulatory factor 1 (Major late
transcription factor 1)
Length = 310
Score = 38.9 bits (89), Expect = 0.013
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 14/95 (14%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSS 114
HP+ ++ ++ A H E E+RRR++IN+ + L ++P S S+
Sbjct: 183 HPYSPKSEAPRTTRDEKRRA---QHNEVERRRRDKINNWIVQLSKIIPDCS-----MEST 234
Query: 115 KSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQ 149
KS +SKG L+K + ++ELRQ ++++
Sbjct: 235 KSG------QSKGGILSKACDYIQELRQSNHRLSE 263
>USF2_RAT (Q63665) Upstream stimulatory factor 2 (Upstream
transcription factor 2) (Major late transcription factor
2) (Fragment)
Length = 291
Score = 38.5 bits (88), Expect = 0.017
Identities = 37/128 (28%), Positives = 57/128 (43%), Gaps = 24/128 (18%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-C---NSKTRQG 110
HP+ +I T + +D A H E E+RRR++IN+ + L ++P C NSKT
Sbjct: 164 HPYS-PKIDGTRTPRDERRRA--QHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTG-- 218
Query: 111 FTSSKSSRTCERFKSKGSRLAKVVERVKELRQ--QTCQITQVETVPCEADGXTVISAGSD 168
SKG L+K + ++ELRQ Q Q T E + D + +
Sbjct: 219 -------------ASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEE 265
Query: 169 MSGEGRLI 176
+ E L+
Sbjct: 266 LKNENALL 273
>USF2_MOUSE (Q64705) Upstream stimulatory factor 2 (Upstream
transcription factor 2) (Major late transcription factor
2)
Length = 346
Score = 38.5 bits (88), Expect = 0.017
Identities = 37/128 (28%), Positives = 57/128 (43%), Gaps = 24/128 (18%)
Query: 55 HPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-C---NSKTRQG 110
HP+ +I T + +D A H E E+RRR++IN+ + L ++P C NSKT
Sbjct: 219 HPYS-PKIDGTRTPRDERRRA--QHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTG-- 273
Query: 111 FTSSKSSRTCERFKSKGSRLAKVVERVKELRQ--QTCQITQVETVPCEADGXTVISAGSD 168
SKG L+K + ++ELRQ Q Q T E + D + +
Sbjct: 274 -------------ASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEE 320
Query: 169 MSGEGRLI 176
+ E L+
Sbjct: 321 LKNENALL 328
>RAP1_ARATH (Q39204) Transcription factor AtMYC2 (R-homologous
Arabidopsis protein-1) (RAP-1) (Basic helix-loop-helix
protein 6) (bHLH6) (AtbHLH006) (rd22BP1)
Length = 623
Score = 38.5 bits (88), Expect = 0.017
Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 11/94 (11%)
Query: 78 NHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVERV 137
NH EAE++RRE++N LR ++P SK + + KSK V
Sbjct: 452 NHVEAERQRREKLNQRFYALRAVVPNVSKMDKASLLGDAIAYINELKSK----------V 501
Query: 138 KELRQQTCQI-TQVETVPCEADGXTVISAGSDMS 170
+ + QI Q+E V E G ++G DMS
Sbjct: 502 VKTESEKLQIKNQLEEVKLELAGRKASASGGDMS 535
>BMAL_RAT (Q9EPW1) Aryl hydrocarbon receptor nuclear
translocator-like protein 1 (Brain and muscle ARNT-like
1) (Tic)
Length = 626
Score = 38.5 bits (88), Expect = 0.017
Identities = 27/87 (31%), Positives = 42/87 (48%), Gaps = 15/87 (17%)
Query: 69 QDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-CNSKTRQGFTSSKSSRTCERFKSKG 127
Q R A + H + EKRRR+++NS +D L +L+P CN+ +R+ K
Sbjct: 67 QGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRK--------------LDKL 112
Query: 128 SRLAKVVERVKELRQQTCQITQVETVP 154
+ L V+ +K LR T T+ P
Sbjct: 113 TVLRMAVQHMKTLRGATNPYTEANYKP 139
>BMAL_MOUSE (Q9WTL8) Aryl hydrocarbon receptor nuclear
translocator-like protein 1 (Brain and muscle ARNT-like
1) (Arnt3)
Length = 632
Score = 38.5 bits (88), Expect = 0.017
Identities = 27/87 (31%), Positives = 42/87 (48%), Gaps = 15/87 (17%)
Query: 69 QDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-CNSKTRQGFTSSKSSRTCERFKSKG 127
Q R A + H + EKRRR+++NS +D L +L+P CN+ +R+ K
Sbjct: 74 QGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRK--------------LDKL 119
Query: 128 SRLAKVVERVKELRQQTCQITQVETVP 154
+ L V+ +K LR T T+ P
Sbjct: 120 TVLRMAVQHMKTLRGATNPYTEANYKP 146
>BMAL_MESAU (O88529) Aryl hydrocarbon receptor nuclear
translocator-like protein 1 (Brain and muscle ARNT-like
1)
Length = 626
Score = 38.5 bits (88), Expect = 0.017
Identities = 27/87 (31%), Positives = 42/87 (48%), Gaps = 15/87 (17%)
Query: 69 QDRALAALKNHKEAEKRRRERINSHLDHLRTLLP-CNSKTRQGFTSSKSSRTCERFKSKG 127
Q R A + H + EKRRR+++NS +D L +L+P CN+ +R+ K
Sbjct: 67 QGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRK--------------LDKL 112
Query: 128 SRLAKVVERVKELRQQTCQITQVETVP 154
+ L V+ +K LR T T+ P
Sbjct: 113 TVLRMAVQHMKTLRGATNPYTEANYKP 139
>USO1_YEAST (P25386) Intracellular protein transport protein USO1
Length = 1790
Score = 37.7 bits (86), Expect = 0.029
Identities = 51/252 (20%), Positives = 113/252 (44%), Gaps = 37/252 (14%)
Query: 30 EYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNHK-------EA 82
EY + S + ++ +++ N+ N ++SE+T T + + LAA KN K E
Sbjct: 1051 EYESQISLLKEKLETATTANDENVN---KISELTKTREELEAELAAYKNLKNELETKLET 1107
Query: 83 EKRRRERINSHLDHLR----TLLPCNSKTRQGFTSSKSSRTC--ERFKSKGSRLAKVVER 136
++ + + + +HL+ L ++T+Q S +++ + + ++L K E+
Sbjct: 1108 SEKALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQ 1167
Query: 137 V-KELRQQTCQITQV--ETVPCEADGXTVISAGSDMSGEGRLIFKASLCCEDRSDLIPDL 193
+ + RQ +I+Q+ E + + ++ ++ GE + + S E++S+L
Sbjct: 1168 IANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMKSTS---EEQSNLKKSE 1224
Query: 194 IEILKSLHLKTLKAEMATLGGRTRNVLVVAAEKEHNSIESIHFLQNSLRSLLDRSSGCND 253
I+ L +L +K LK + T E + +ESI +++ + + CN
Sbjct: 1225 IDAL-NLQIKELKKKNET--------------NEASLLESIKSVESETVKIKELQDECNF 1269
Query: 254 RSKRRRGLDRRM 265
+ K L+ ++
Sbjct: 1270 KEKEVSELEDKL 1281
>HAIR_DROME (P14003) Hairy protein
Length = 337
Score = 36.2 bits (82), Expect = 0.085
Identities = 26/90 (28%), Positives = 43/90 (46%), Gaps = 13/90 (14%)
Query: 60 SEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSSKSSRT 119
+++ +TP + DR N EKRRR RIN+ L+ L+TL+ ++K
Sbjct: 21 AQLKETPLKSDRR----SNKPIMEKRRRARINNCLNELKTLI---------LDATKKDPA 67
Query: 120 CERFKSKGSRLAKVVERVKELRQQTCQITQ 149
K L K V+ ++EL++Q + Q
Sbjct: 68 RHSKLEKADILEKTVKHLQELQRQQAAMQQ 97
>CYCL_DROME (O61734) Cycle protein (Brain and muscle ARNT-like 1)
(BMAL1) (MOP3)
Length = 413
Score = 36.2 bits (82), Expect = 0.085
Identities = 18/65 (27%), Positives = 36/65 (54%), Gaps = 7/65 (10%)
Query: 38 MMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHL 97
M Q FC + + + + E S T +++ +NH E EKRRR+++N++++ L
Sbjct: 1 MEVQEFCENMEEIEDENYDEEKSARTSDENRK-------QNHSEIEKRRRDKMNTYINEL 53
Query: 98 RTLLP 102
+++P
Sbjct: 54 SSMIP 58
>PRE_STRAG (P13925) Plasmid recombination enzyme (Mobilization
protein)
Length = 494
Score = 35.8 bits (81), Expect = 0.11
Identities = 54/228 (23%), Positives = 93/228 (40%), Gaps = 19/228 (8%)
Query: 57 FEV-SEITDTPSQQDRALAALKNHKEAEKRRRER--INSHLDHLRTLLPCNSKTRQGFTS 113
FEV S I +T +Q+ + +E +K + + L HL LL +K S
Sbjct: 239 FEVQSPIRETTNQEKMDWLRKQYQEELKKLESSKKPLEDDLSHLEELLDKKTKEYIKIDS 298
Query: 114 SKSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQVETVPCEADGXTVISAGSDMSGEG 173
S R E K++G +E + + + + + + E T + A + E
Sbjct: 299 EASERASELSKAEG--YINTLENHSKSLEAKIECLESDNLQLEKQKATKLEAKALNESEL 356
Query: 174 R-LIFKASLCCEDRSDLIPDLIEILKS--------LHLKTLKAEMATLGGRTR---NVLV 221
R L K + ++ +L P+ E LK+ LH K ++ E A R N
Sbjct: 357 RELKPKKNFLGKEHYELSPEQFEGLKAEVYRSRTLLHHKDIELEQAKRQVSLRASKNYFT 416
Query: 222 VAAE--KEHNSIESIHFLQNSLRSLLDRSSGCNDRSKRRRGLDRRMMP 267
+ E KE ESI L++ ++ L + +S ++ + G R +MP
Sbjct: 417 ASLERAKEKAKGESIDRLKSEIKRLKNENSILRQQNDKMLGKLRELMP 464
>CBF1_YEAST (P17106) Centromere-binding protein 1 (CBP-1)
(Centromere-binding factor 1) (Centromere promoter
factor 1)
Length = 351
Score = 35.8 bits (81), Expect = 0.11
Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 15/95 (15%)
Query: 78 NHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVERV 137
+HKE E+RRRE IN+ ++ L LLP ++ +R A+ ++++
Sbjct: 226 SHKEVERRRRENINTAINVLSDLLPVRESSKAAIL---------------ARAAEYIQKL 270
Query: 138 KELRQQTCQITQVETVPCEADGXTVISAGSDMSGE 172
KE + + ++ + E + + SA + E
Sbjct: 271 KETDEANIEKWTLQKLLSEQNASQLASANEKLQEE 305
>CBF1_KLULA (P49379) Centromere-binding protein 1 (CBP-1)
(Centromere-binding factor 1) (Centromere promoter
factor 1)
Length = 359
Score = 35.0 bits (79), Expect = 0.19
Identities = 22/96 (22%), Positives = 43/96 (43%), Gaps = 15/96 (15%)
Query: 77 KNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVER 136
++HKE E+RRR+ IN+ ++ L LLP ++ SR A+ +++
Sbjct: 252 ESHKEVERRRRQNINTAIEKLSDLLPVKETSKAAIL---------------SRAAEYIQK 296
Query: 137 VKELRQQTCQITQVETVPCEADGXTVISAGSDMSGE 172
+KE + ++ + E ++ SA + E
Sbjct: 297 MKETETANIEKWTLQKLLGEQQVSSLTSANDKLEQE 332
>NUC1_NEUCR (P20824) Phosphorus acquisition controlling protein
Length = 823
Score = 34.7 bits (78), Expect = 0.25
Identities = 36/141 (25%), Positives = 59/141 (41%), Gaps = 31/141 (21%)
Query: 66 PSQQDRALAALK-NHKEAEKRRRERINSHLDHLRTLLP-------------CNSKTRQGF 111
PS+ L + + +HK AE+ RR RINS L + TLLP +S + G
Sbjct: 658 PSELSTNLTSKRTSHKIAEQGRRNRINSALQEIATLLPKAPAKEGGDGDGDGHSSSGGGG 717
Query: 112 TSSKSSRTCERFK-----------SKGSRLAKVVERVKELRQQT------CQITQVETVP 154
S + R +R K SK S + +E +K+L+++ + + + V
Sbjct: 718 GSGGADREDKREKDKDKAGGGIPNSKASTVEMAIEYIKQLQKEVADANKRAEEAERKLVE 777
Query: 155 CEADGXTVISAGSDMSGEGRL 175
+ G +GS + G L
Sbjct: 778 MKMQGGAATGSGSSVGDAGDL 798
>USF_STRPU (Q07956) Upstream stimulatory factor (USF) (SPF1)
Length = 265
Score = 34.3 bits (77), Expect = 0.32
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 15/77 (19%)
Query: 79 HKEAEKRRRERINSHLDHLRTLLP-CN-SKTRQGFTSSKSSRTCERFKSKGSRLAKVVER 136
H E E+RRR++IN+ + L ++P CN ++QG +SKG L K +
Sbjct: 195 HNEVERRRRDKINNWIVKLSKIIPDCNIDHSKQG-------------QSKGGILTKTCDY 241
Query: 137 VKELRQQTCQITQVETV 153
+ +LR ++ + +
Sbjct: 242 IHDLRNSNTRMAKASRI 258
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.129 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,287,883
Number of Sequences: 164201
Number of extensions: 1010572
Number of successful extensions: 3724
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 38
Number of HSP's that attempted gapping in prelim test: 3674
Number of HSP's gapped (non-prelim): 80
length of query: 267
length of database: 59,974,054
effective HSP length: 108
effective length of query: 159
effective length of database: 42,240,346
effective search space: 6716215014
effective search space used: 6716215014
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 65 (29.6 bits)
Medicago: description of AC135795.6