BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135795.3 + phase: 0 
         (134 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

WR19_ARATH (Q9SZ67) Probable WRKY transcription factor 19 (WRKY ...    62  3e-10
SN3A_HUMAN (Q96ST3) Paired amphipathic helix protein Sin3a             55  2e-08
SN3A_MOUSE (Q60520) Paired amphipathic helix protein Sin3a             54  8e-08
SIN3_YEAST (P22579) Paired amphipathic helix protein SIN3              52  2e-07
SN3B_MOUSE (Q62141) Paired amphipathic helix protein Sin3b             49  1e-06
SN3B_HUMAN (O75182) Paired amphipathic helix protein Sin3b             49  1e-06
PST1_SCHPO (Q09750) Paired amphipathic helix protein pst1 (SIN3 ...    46  1e-05
GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing prote...    36  0.013
GCC2_MOUSE (Q8CHG3) GRIP and coiled-coil domain-containing prote...    35  0.022
CH60_METMA (Q8PW06) 60 kDa chaperonin (Protein Cpn60) (groEL pro...    35  0.022
CLPB_HELPJ (Q9ZMH1) Chaperone clpB                                     34  0.049
LPB1_HUMAN (Q86W92) Liprin-beta 1 (Protein tyrosine phosphatase ...    34  0.063
CLPB_HELPY (P71404) Chaperone clpB                                     34  0.063
OSB2_HUMAN (Q969R2) Oxysterol-binding protein 2 (Oxysterol bindi...    33  0.083
NUF1_YEAST (P32380) NUF1 protein (Spindle poly body spacer prote...    33  0.11
CH60_METAC (Q8TGX7) 60 kDa chaperonin (Protein Cpn60) (groEL pro...    33  0.14
RA50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase        32  0.32
Y4FB_RHISN (P55440) Hypothetical 73.7 kDa protein y4fB                 31  0.41
BA29_MOUSE (Q61334) B-cell receptor-associated protein 29 (BCR-a...    31  0.41
Y211_MYCPN (P75481) Hypothetical protein MG211 homolog (H10_orf149)    30  0.70

>WR19_ARATH (Q9SZ67) Probable WRKY transcription factor 19 (WRKY
           DNA-binding protein 19)
          Length = 1895

 Score = 61.6 bits (148), Expect = 3e-10
 Identities = 36/88 (40%), Positives = 52/88 (58%), Gaps = 4/88 (4%)

Query: 27  DALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNS 86
           DAL ++K V+ +FED  +KYD FL V+ND K   +D  G  ARL +LFK H  L++ FN+
Sbjct: 308 DALNYLKAVKDKFEDS-EKYDTFLEVLNDCKHQGVDTSGVIARLKDLFKGHDDLLLGFNT 366

Query: 87  LTLKGSHFTKHGYETEEEEDMEILDKLE 114
              K    T      E++  ++ LDK+E
Sbjct: 367 YLSKEYQIT---ILPEDDFPIDFLDKVE 391


>SN3A_HUMAN (Q96ST3) Paired amphipathic helix protein Sin3a
          Length = 1273

 Score = 55.5 bits (132), Expect = 2e-08
 Identities = 28/78 (35%), Positives = 48/78 (60%), Gaps = 7/78 (8%)

Query: 25  MKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRF 84
           ++DAL+++  V+++F  +   Y++FL++M +FK+  ID  G  +R+ +LFK H  LIM F
Sbjct: 123 VEDALSYLDQVKLQFGSQPQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGF 182

Query: 85  NSLTLKGSHFTKHGYETE 102
           N+       F   GY+ E
Sbjct: 183 NT-------FLPPGYKIE 193


>SN3A_MOUSE (Q60520) Paired amphipathic helix protein Sin3a
          Length = 1282

 Score = 53.5 bits (127), Expect = 8e-08
 Identities = 27/78 (34%), Positives = 48/78 (60%), Gaps = 7/78 (8%)

Query: 25  MKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRF 84
           ++DAL+++  V+++F  +   +++FL++M +FK+  ID  G  +R+ +LFK H  LIM F
Sbjct: 123 VEDALSYLDQVKLQFGSQPQVHNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGF 182

Query: 85  NSLTLKGSHFTKHGYETE 102
           N+       F   GY+ E
Sbjct: 183 NT-------FLPPGYKIE 193


>SIN3_YEAST (P22579) Paired amphipathic helix protein SIN3
          Length = 1536

 Score = 52.4 bits (124), Expect = 2e-07
 Identities = 25/68 (36%), Positives = 43/68 (62%)

Query: 24  SMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMR 83
           ++KDAL++++ V+ +F  + D Y+ FL++M DFK+  ID  G   R+  LF+ +  LI  
Sbjct: 220 NVKDALSYLEQVKFQFSSRPDIYNLFLDIMKDFKSQAIDTPGVIERVSTLFRGYPILIQG 279

Query: 84  FNSLTLKG 91
           FN+   +G
Sbjct: 280 FNTFLPQG 287



 Score = 27.7 bits (60), Expect = 4.5
 Identities = 13/57 (22%), Positives = 28/57 (48%)

Query: 28  ALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRF 84
           A+++V  ++  F D+ D Y  FL ++  ++  +       A++  LF+    L+  F
Sbjct: 411 AISYVNKIKTRFADQPDIYKHFLEILQTYQREQKPINEVYAQVTHLFQNAPDLLEDF 467


>SN3B_MOUSE (Q62141) Paired amphipathic helix protein Sin3b
          Length = 954

 Score = 49.3 bits (116), Expect = 1e-06
 Identities = 23/62 (37%), Positives = 37/62 (59%)

Query: 25 MKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRF 84
          ++DAL ++  V++ F      Y+ FL +M +FK+  ID  G   R+ +LF EH  LI+ F
Sbjct: 34 VEDALTYLDQVKIRFGSDPATYNGFLEIMKEFKSQSIDTPGVIRRVSQLFHEHPDLIVGF 93

Query: 85 NS 86
          N+
Sbjct: 94 NA 95


>SN3B_HUMAN (O75182) Paired amphipathic helix protein Sin3b
          Length = 1130

 Score = 49.3 bits (116), Expect = 1e-06
 Identities = 23/62 (37%), Positives = 37/62 (59%)

Query: 25  MKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRF 84
           ++DAL ++  V++ F      Y+ FL +M +FK+  ID  G   R+ +LF EH  LI+ F
Sbjct: 41  VEDALTYLDQVKIRFGSDPATYNGFLEIMKEFKSQSIDTPGVIRRVSQLFHEHPDLIVGF 100

Query: 85  NS 86
           N+
Sbjct: 101 NA 102


>PST1_SCHPO (Q09750) Paired amphipathic helix protein pst1 (SIN3
           homolog 1)
          Length = 1522

 Score = 46.2 bits (108), Expect = 1e-05
 Identities = 22/70 (31%), Positives = 43/70 (61%)

Query: 22  QQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLI 81
           Q ++ DAL+++  V+++F  + + Y+EFL++M +FK+  I+      R+ +LF  + +LI
Sbjct: 179 QLNVTDALSYLDLVKLQFHQEPEIYNEFLDIMKEFKSQAIETPEVITRVSKLFAGYPNLI 238

Query: 82  MRFNSLTLKG 91
             FN+    G
Sbjct: 239 QGFNTFLPPG 248


>GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing protein
           2 (Golgi coiled coil protein GCC185) (CTCL tumor antigen
           se1-1) (CLL-associated antigen KW-11)
          Length = 1583

 Score = 36.2 bits (82), Expect = 0.013
 Identities = 30/135 (22%), Positives = 67/135 (49%), Gaps = 5/135 (3%)

Query: 1   MNQIKLIILLAMKEYGYAHQNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLR 60
           +N+IKL+ + A KE   + +  Q++K+ L   + +  E +       + +     +K L 
Sbjct: 866 INKIKLVAVKAKKELDSSRKETQTVKEEL---ESLRSEKDQLSASMRDLIQGAESYKNLL 922

Query: 61  IDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLESAL 120
           ++ E    +LD   +   +   R   LT +  + T    ET   ++ ++L ++E L+S  
Sbjct: 923 LEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQ-CETINSDNEDLLARIETLQSNA 981

Query: 121 QLI-LEILVIKKKEA 134
           +L+ ++IL +++ +A
Sbjct: 982 KLLEVQILEVQRAKA 996



 Score = 32.0 bits (71), Expect = 0.24
 Identities = 27/121 (22%), Positives = 50/121 (41%), Gaps = 6/121 (4%)

Query: 5   KLIILLAMKEYGYAHQNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAE 64
           +LI+ L  K       N +  +       G+E   ++K     +   +M   K L  D E
Sbjct: 521 RLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKE 580

Query: 65  GAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLESALQLIL 124
              A +  L++E+  L      L+     F        ++ED+ + + + +LE  LQL++
Sbjct: 581 VLSAEVKSLYEENNKLSSEKKQLSRDLEVFLS------QKEDVILKEHITQLEKKLQLMV 634

Query: 125 E 125
           E
Sbjct: 635 E 635


>GCC2_MOUSE (Q8CHG3) GRIP and coiled-coil domain-containing protein 2
            (Golgi coiled coil protein GCC185)
          Length = 1679

 Score = 35.4 bits (80), Expect = 0.022
 Identities = 31/132 (23%), Positives = 64/132 (48%), Gaps = 5/132 (3%)

Query: 1    MNQIKLIILLAMKEYGYAHQNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLR 60
            +++IKL+ + A KE     +  Q++++ L  V+    E +       EFL     +K+L 
Sbjct: 958  ISKIKLVAVKAKKELDSNRKEAQTLREELESVRS---EKDRLSASMKEFLQGAESYKSLL 1014

Query: 61   IDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLESAL 120
            ++ +    +LD + KE  H   R      K    +   YE    ++ ++L ++E L++  
Sbjct: 1015 LEYDKQSEQLD-VEKERAHNFERHIEDLTKQLRNSTCQYERLTSDNEDLLARIETLQANA 1073

Query: 121  QLI-LEILVIKK 131
            +L+  +IL ++K
Sbjct: 1074 KLLEAQILEVQK 1085


>CH60_METMA (Q8PW06) 60 kDa chaperonin (Protein Cpn60) (groEL
           protein)
          Length = 536

 Score = 35.4 bits (80), Expect = 0.022
 Identities = 21/71 (29%), Positives = 41/71 (57%), Gaps = 10/71 (14%)

Query: 11  AMKEYGYAHQNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVM---------NDFKTLRI 61
           A+K  G+ ++ ++ ++D +A + G +V  E+KG K +EF + M         ++ KT+ +
Sbjct: 274 AVKAPGFGNERKEMLED-IAVLTGGQVISEEKGMKLEEFSDYMLGNARKVTVDNHKTIIV 332

Query: 62  DAEGAKARLDE 72
           +  G KA +DE
Sbjct: 333 EGRGDKADIDE 343


>CLPB_HELPJ (Q9ZMH1) Chaperone clpB
          Length = 856

 Score = 34.3 bits (77), Expect = 0.049
 Identities = 25/91 (27%), Positives = 41/91 (44%), Gaps = 2/91 (2%)

Query: 33  KGVEVEFEDKGDK-YDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKG 91
           + +E+E ++   K   E L  ++D K  +I  E       E+FKE   L M    L  + 
Sbjct: 424 QALEMENKESNHKRMQEILKELSDLKEEKIQLEAQFENEKEVFKEISRLKMEMEGLKKEA 483

Query: 92  SHFTKHG-YETEEEEDMEILDKLEKLESALQ 121
             F ++G Y+   E +   + + EK E  LQ
Sbjct: 484 ERFKRNGDYQQAAEIEYSKIPEKEKKEKELQ 514


>LPB1_HUMAN (Q86W92) Liprin-beta 1 (Protein tyrosine phosphatase
           receptor type f polypeptide-interacting protein binding
           protein 1) (PTPRF-interacting protein binding protein 1)
           (hSGT2)
          Length = 1011

 Score = 33.9 bits (76), Expect = 0.063
 Identities = 23/115 (20%), Positives = 57/115 (49%), Gaps = 6/115 (5%)

Query: 24  SMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMR 83
           ++K  L  V+   +++EDK    +  +  +ND +    + +  + + ++  K  + L+ +
Sbjct: 178 NLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAK 237

Query: 84  FNSLTLKGSHF------TKHGYETEEEEDMEILDKLEKLESALQLILEILVIKKK 132
            +S+ +K           +  +E+ ++E   + ++LE+ ES ++ + E LV K K
Sbjct: 238 LSSMKIKVGQMQYEKQRMEQKWESLKDELASLKEQLEEKESEVKRLQEKLVCKMK 292


>CLPB_HELPY (P71404) Chaperone clpB
          Length = 856

 Score = 33.9 bits (76), Expect = 0.063
 Identities = 23/83 (27%), Positives = 36/83 (42%), Gaps = 1/83 (1%)

Query: 40  EDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHG- 98
           E    +  E L  ++D K  +I  E       E+FKE   L M   SL  +   F ++G 
Sbjct: 432 ESNAKRMQEILKELSDLKEEKIQLEAQFENEKEVFKEISRLKMEMESLKKEAERFKRNGD 491

Query: 99  YETEEEEDMEILDKLEKLESALQ 121
           Y+   E +   + + +K E  LQ
Sbjct: 492 YQQAGEIEYSKIPENKKKEEELQ 514


>OSB2_HUMAN (Q969R2) Oxysterol-binding protein 2 (Oxysterol binding
           protein-related protein 4) (OSBP-related protein 4)
           (ORP-4)
          Length = 878

 Score = 33.5 bits (75), Expect = 0.083
 Identities = 20/66 (30%), Positives = 33/66 (49%), Gaps = 10/66 (15%)

Query: 70  LDELFKEHRHLIMRFNSLT----------LKGSHFTKHGYETEEEEDMEILDKLEKLESA 119
           +++L K+H  L   F+S            ++GS  T  G ++EE+ED E  D +E   S 
Sbjct: 365 IEQLAKQHNSLERAFHSAPGRPANPSKSFIEGSLLTPKGEDSEEDEDTEYFDAMEDSTSF 424

Query: 120 LQLILE 125
           + +I E
Sbjct: 425 ITVITE 430


>NUF1_YEAST (P32380) NUF1 protein (Spindle poly body spacer protein
           SPC110)
          Length = 944

 Score = 33.1 bits (74), Expect = 0.11
 Identities = 26/98 (26%), Positives = 42/98 (42%), Gaps = 9/98 (9%)

Query: 29  LAFVKGVEVEFEDKGD--------KYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHL 80
           L  VK   +E E+  D        K DE  N+MN+   L+ +AE    +L+    E R  
Sbjct: 243 LKTVKDQVLELENNSDVQSLKLRSKEDELKNLMNELNELKSNAEEKDTQLEFKKNELRKR 302

Query: 81  IMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLES 118
               N L +K         + ++ E   + D+L +LE+
Sbjct: 303 TNELNELKIKSDEMDLQ-LKQKQNESKRLKDELNELET 339


>CH60_METAC (Q8TGX7) 60 kDa chaperonin (Protein Cpn60) (groEL
           protein)
          Length = 536

 Score = 32.7 bits (73), Expect = 0.14
 Identities = 19/71 (26%), Positives = 41/71 (56%), Gaps = 10/71 (14%)

Query: 11  AMKEYGYAHQNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVM---------NDFKTLRI 61
           A+K  G+ ++ ++ ++D +A + G +V  E+KG K +EF + M         ++ KT+ +
Sbjct: 274 AVKAPGFGNERKEMLED-IAVLTGGQVISEEKGMKLEEFSDYMLGSARKVTVDNNKTIIV 332

Query: 62  DAEGAKARLDE 72
           +  G K +++E
Sbjct: 333 EGRGDKEKIEE 343


>RA50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase
          Length = 880

 Score = 31.6 bits (70), Expect = 0.32
 Identities = 27/90 (30%), Positives = 37/90 (41%)

Query: 38  EFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKH 97
           E E+  DKY E     ++ + L+   E  K  LD+ F+    +         K       
Sbjct: 597 ELEEFHDKYVEAKKSESELRELKNKLEKEKTELDQAFEMLADVENEIEEKEAKLKDLESK 656

Query: 98  GYETEEEEDMEILDKLEKLESALQLILEIL 127
             E E EE  E L KLE+  S+L   LE L
Sbjct: 657 FNEEEYEEKRERLVKLEREVSSLTARLEEL 686


>Y4FB_RHISN (P55440) Hypothetical 73.7 kDa protein y4fB
          Length = 664

 Score = 31.2 bits (69), Expect = 0.41
 Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 8/84 (9%)

Query: 24  SMKDALAF-VKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIM 82
           S +DAL + V  +E   ED           ++DF  LR+DA   K  + +     +H   
Sbjct: 147 SPRDALDYTVVAIEENSEDGATP-------ISDFGFLRLDARTGKTEVGQYATIIQHPDA 199

Query: 83  RFNSLTLKGSHFTKHGYETEEEED 106
           +   ++L+ + F K+G E + E D
Sbjct: 200 KTKRISLRENKFVKYGDEADPERD 223


>BA29_MOUSE (Q61334) B-cell receptor-associated protein 29
           (BCR-associated protein Bap29)
          Length = 240

 Score = 31.2 bits (69), Expect = 0.41
 Identities = 16/45 (35%), Positives = 19/45 (41%)

Query: 47  DEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKG 91
           D  L   ND  T++I +E      D L KEH  L  R      KG
Sbjct: 195 DALLKAQNDVMTMKIQSERLSKEYDRLLKEHSELQNRLEKEKKKG 239


>Y211_MYCPN (P75481) Hypothetical protein MG211 homolog (H10_orf149)
          Length = 149

 Score = 30.4 bits (67), Expect = 0.70
 Identities = 19/92 (20%), Positives = 45/92 (48%), Gaps = 13/92 (14%)

Query: 47  DEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEE--- 103
           D+ ++ +  ++  R   E  K   ++LF++   ++ R + L  + ++F ++GY       
Sbjct: 40  DQLVHTLTHYEAQRNREEEMKTAYEKLFQDRDEILKRCSKLEAELNNFYENGYSNRVLIS 99

Query: 104 -----EEDMEIL-----DKLEKLESALQLILE 125
                E  +E L     D+LE++E  L+ +++
Sbjct: 100 RVQALENKIESLPSGQNDRLERIEKLLKRVIK 131


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.318    0.136    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,480,246
Number of Sequences: 164201
Number of extensions: 561491
Number of successful extensions: 2181
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 48
Number of HSP's that attempted gapping in prelim test: 2124
Number of HSP's gapped (non-prelim): 103
length of query: 134
length of database: 59,974,054
effective HSP length: 110
effective length of query: 24
effective length of database: 41,911,944
effective search space: 1005886656
effective search space used: 1005886656
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)