BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135505.7 - phase: 0 /pseudo
(109 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DNAK_CHLCV (Q824B2) Chaperone protein dnaK (Heat shock protein 7... 42 3e-04
DNAK_CHLAB (Q8GH79) Chaperone protein dnaK (Heat shock protein 7... 42 3e-04
HS88_NEUCR (O74225) Heat shock protein Hsp88 41 4e-04
GR78_SCHPO (P36604) 78 kDa glucose-regulated protein homolog pre... 41 4e-04
DNK1_SYNP7 (P50020) Chaperone protein dnaK1 (Heat shock protein ... 41 4e-04
DNK1_SYNY3 (Q55154) Chaperone protein dnaK1 (Heat shock protein ... 41 6e-04
YHM4_YEAST (P38788) Heat shock protein 70 homolog YHR064C 40 7e-04
HSCC_ECOLI (P77319) Chaperone protein hscC (Hsc62) 40 0.001
DNK1_SYNEL (Q8DKR6) Chaperone protein dnaK1 (Heat shock protein ... 40 0.001
OS94_MOUSE (P48722) Osmotic stress protein 94 (Heat shock 70-rel... 39 0.002
OS94_HUMAN (O95757) Osmotic stress protein 94 (Heat shock 70-rel... 39 0.002
DNAK_FUSNN (Q8RH05) Chaperone protein dnaK (Heat shock protein 7... 39 0.002
HS7M_SOLTU (Q08276) Heat shock 70 kDa protein, mitochondrial pre... 39 0.003
HS7E_DROME (P29845) Heat shock 70 kDa protein cognate 5 39 0.003
HS75_SCHPO (Q10284) Heat shock protein sks2 (Heat shock cognate ... 39 0.003
HS70_ENCCU (O96772) Mitochondrial-type heat shock protein 70 (mi... 39 0.003
DNAK_METTH (O27351) Chaperone protein dnaK (Heat shock protein 7... 39 0.003
DNAK_CHLTR (P17821) Chaperone protein dnaK (Heat shock protein 7... 39 0.003
DNAK_CHLPN (P27542) Chaperone protein dnaK (Heat shock protein 7... 39 0.003
DNAK_CHLMU (P56836) Chaperone protein dnaK (Heat shock protein 7... 39 0.003
>DNAK_CHLCV (Q824B2) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 664
Score = 42.0 bits (97), Expect = 3e-04
Identities = 23/69 (33%), Positives = 36/69 (51%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ + L+K+K + E + P+ + V TVP F +R + A A LD ++P
Sbjct: 114 TPEEIGAQVLIKMKETAEAYLGEPVTEAVITVPAYFNDSQRASTKDAGRIAGLDVKRIIP 173
Query: 84 QPIAVALFY 92
+P A AL Y
Sbjct: 174 EPTAAALAY 182
>DNAK_CHLAB (Q8GH79) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 659
Score = 42.0 bits (97), Expect = 3e-04
Identities = 23/69 (33%), Positives = 36/69 (51%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ + L+K+K + E + P+ + V TVP F +R + A A LD ++P
Sbjct: 114 TPEEIGAQVLIKMKETAEAYLGEPVTEAVITVPAYFNDSQRASTKDAGRIAGLDVKRIIP 173
Query: 84 QPIAVALFY 92
+P A AL Y
Sbjct: 174 EPTAAALAY 182
>HS88_NEUCR (O74225) Heat shock protein Hsp88
Length = 707
Score = 41.2 bits (95), Expect = 4e-04
Identities = 23/70 (32%), Positives = 39/70 (54%)
Query: 21 RSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVE 80
R +A EL+ F+ K+K + + VK+ ++++V +VP FT +R I A+ A L +
Sbjct: 109 RKFSATELIAMFMSKIKQTTQAEVKVAVQELVLSVPAWFTDKQRRSILDAAEIAGLRPLR 168
Query: 81 LMPQPIAVAL 90
L+ A AL
Sbjct: 169 LINDTTAAAL 178
>GR78_SCHPO (P36604) 78 kDa glucose-regulated protein homolog
precursor (GRP 78) (Immunoglobulin heavy chain binding
protein homolog) (BiP)
Length = 663
Score = 41.2 bits (95), Expect = 4e-04
Identities = 25/98 (25%), Positives = 43/98 (43%)
Query: 2 HTLDIKVRPFIAAIANKACRSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTR 61
H ++ K RP + + T E+ L K+K + E + P+ V TVP F
Sbjct: 124 HIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFND 183
Query: 62 LRRTQIERASAWADLDDVELMPQPIAVALFYAQQQLQT 99
+R + A A L+ + ++ +P A A+ Y + T
Sbjct: 184 AQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT 221
>DNK1_SYNP7 (P50020) Chaperone protein dnaK1 (Heat shock protein
70-1) (Heat shock 70 kDa protein 1) (HSP70-1)
Length = 655
Score = 41.2 bits (95), Expect = 4e-04
Identities = 26/84 (30%), Positives = 41/84 (47%)
Query: 21 RSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVE 80
R T E+ L KL+ E + P+ + V TVP FT +R + A A A L+ +
Sbjct: 106 RFCTPQEISAMVLQKLRQDAETFLGEPVTQAVITVPAYFTDAQRQATKDAGAIAGLEVLR 165
Query: 81 LMPQPIAVALFYAQQQLQTSASSL 104
++ +P A AL Y +L ++ L
Sbjct: 166 IVNEPTAAALSYGLDKLHENSRIL 189
>DNK1_SYNY3 (Q55154) Chaperone protein dnaK1 (Heat shock protein
70-1) (Heat shock 70 kDa protein 1) (HSP70-1)
Length = 692
Score = 40.8 bits (94), Expect = 6e-04
Identities = 25/79 (31%), Positives = 39/79 (48%)
Query: 21 RSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVE 80
+S T E+ L KLK E + P+ + V TVP FT +R + A A L+ +
Sbjct: 106 QSYTPQEISAMILQKLKADSEAFLGEPVTQAVITVPAYFTDAQRQATKDAGTIAGLEVLR 165
Query: 81 LMPQPIAVALFYAQQQLQT 99
++ +P A AL Y + +T
Sbjct: 166 IINEPTAAALAYGLDKQET 184
>YHM4_YEAST (P38788) Heat shock protein 70 homolog YHR064C
Length = 572
Score = 40.4 bits (93), Expect = 7e-04
Identities = 20/72 (27%), Positives = 42/72 (57%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E++ + L +LK + E + +++ V TVP +F+ ++T ++ ++A L V+ +
Sbjct: 148 TVDEVVSRHLNRLKLAAEDYIGSAVKEAVLTVPTNFSEEQKTALKASAAKIGLQIVQFIN 207
Query: 84 QPIAVALFYAQQ 95
+P A L +A+Q
Sbjct: 208 EPSAALLAHAEQ 219
>HSCC_ECOLI (P77319) Chaperone protein hscC (Hsc62)
Length = 556
Score = 39.7 bits (91), Expect = 0.001
Identities = 27/83 (32%), Positives = 41/83 (48%)
Query: 25 AVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQ 84
A EL L LK E ++ PI+ VV +VP F+ +R A+ A L+ V L+ +
Sbjct: 91 APELSSLVLRSLKEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINE 150
Query: 85 PIAVALFYAQQQLQTSASSLEDM 107
P A A+ Y Q + S + D+
Sbjct: 151 PTAAAMAYGLHTQQNTRSLVFDL 173
>DNK1_SYNEL (Q8DKR6) Chaperone protein dnaK1 (Heat shock protein
70-1) (Heat shock 70 kDa protein 1) (HSP70-1)
Length = 651
Score = 39.7 bits (91), Expect = 0.001
Identities = 27/82 (32%), Positives = 41/82 (49%), Gaps = 3/82 (3%)
Query: 21 RSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVE 80
R+ T E+ L KLK E + P+ + V TVP F+ +R + A A A L+ +
Sbjct: 106 RTYTPQEISAMVLQKLKQDAEAYLGEPVTQAVITVPAYFSDAQRQATKDAGAIAGLEVLR 165
Query: 81 LMPQPIAVALFYA---QQQLQT 99
++ +P A +L Y Q Q QT
Sbjct: 166 IINEPTAASLAYGIDKQDQDQT 187
>OS94_MOUSE (P48722) Osmotic stress protein 94 (Heat shock
70-related protein APG-1)
Length = 838
Score = 39.3 bits (90), Expect = 0.002
Identities = 23/66 (34%), Positives = 35/66 (52%)
Query: 27 ELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQPI 86
++ G L KLK + E +K P+ V ++P FT R + A+ A L+ + LM +
Sbjct: 116 QVTGMLLAKLKETSENALKKPVADCVISIPSFFTDAERRSVMAAAQVAGLNCLRLMNETT 175
Query: 87 AVALFY 92
AVAL Y
Sbjct: 176 AVALAY 181
>OS94_HUMAN (O95757) Osmotic stress protein 94 (Heat shock
70-related protein APG-1)
Length = 839
Score = 39.3 bits (90), Expect = 0.002
Identities = 23/66 (34%), Positives = 35/66 (52%)
Query: 27 ELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQPI 86
++ G L KLK + E +K P+ V ++P FT R + A+ A L+ + LM +
Sbjct: 116 QVTGMLLAKLKETSENALKKPVADCVISIPSFFTDAERRSVMAAAQVAGLNCLRLMNETT 175
Query: 87 AVALFY 92
AVAL Y
Sbjct: 176 AVALAY 181
>DNAK_FUSNN (Q8RH05) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 607
Score = 38.9 bits (89), Expect = 0.002
Identities = 24/73 (32%), Positives = 36/73 (48%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ K L KLK E + +++ V TVP FT +R + A A LD ++
Sbjct: 87 TPQEISAKTLQKLKKDAEAYLGEEVKEAVITVPAYFTDSQRQATKDAGTIAGLDVKRIIN 146
Query: 84 QPIAVALFYAQQQ 96
+P A AL Y ++
Sbjct: 147 EPTAAALAYGLEK 159
>HS7M_SOLTU (Q08276) Heat shock 70 kDa protein, mitochondrial
precursor
Length = 682
Score = 38.5 bits (88), Expect = 0.003
Identities = 25/65 (38%), Positives = 34/65 (51%), Gaps = 1/65 (1%)
Query: 29 LGKF-LVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQPIA 87
+G F L K+K + E + I K V TVP F +R I+ A A A LD ++ +P A
Sbjct: 170 IGAFILTKMKETAEAYLGKSINKAVITVPAYFNDAQRQAIKDAGAIAGLDVQRIINEPTA 229
Query: 88 VALFY 92
AL Y
Sbjct: 230 AALSY 234
>HS7E_DROME (P29845) Heat shock 70 kDa protein cognate 5
Length = 686
Score = 38.5 bits (88), Expect = 0.003
Identities = 22/80 (27%), Positives = 41/80 (50%), Gaps = 1/80 (1%)
Query: 29 LGKF-LVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQPIA 87
+G F L+K+K + E + P++ V TVP F +R + A A L+ + ++ +P A
Sbjct: 166 IGAFILMKMKETAEAYLNTPVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVINEPTA 225
Query: 88 VALFYAQQQLQTSASSLEDM 107
AL Y + + ++ D+
Sbjct: 226 AALAYGMDKTEDKIIAVYDL 245
>HS75_SCHPO (Q10284) Heat shock protein sks2 (Heat shock cognate
protein hsc1)
Length = 613
Score = 38.5 bits (88), Expect = 0.003
Identities = 22/69 (31%), Positives = 36/69 (51%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
TA E+ L K+K E ++ + K V TVP F+ +R + A A A L+ + ++
Sbjct: 117 TAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIIN 176
Query: 84 QPIAVALFY 92
+P A A+ Y
Sbjct: 177 EPTAAAIAY 185
>HS70_ENCCU (O96772) Mitochondrial-type heat shock protein 70
(mit-hsp70)
Length = 592
Score = 38.5 bits (88), Expect = 0.003
Identities = 24/65 (36%), Positives = 35/65 (52%), Gaps = 1/65 (1%)
Query: 29 LGKFLV-KLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMPQPIA 87
+G F++ KLK S E + P+ + V TVP F +R + A A LD V ++ +P A
Sbjct: 125 IGAFVLSKLKSSAEAFLSHPVARSVITVPAYFNDSQRQATKDAGRIAGLDVVRVINEPTA 184
Query: 88 VALFY 92
AL Y
Sbjct: 185 AALAY 189
>DNAK_METTH (O27351) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 596
Score = 38.5 bits (88), Expect = 0.003
Identities = 25/69 (36%), Positives = 34/69 (49%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ L K+K E + I+K V TVP F +RT + A A LD V L+
Sbjct: 94 TPQEISAFILQKIKKDAEAFLGEEIKKAVITVPAYFDDNQRTATKDAGTIAGLDVVRLVN 153
Query: 84 QPIAVALFY 92
+P A +L Y
Sbjct: 154 EPTAASLAY 162
>DNAK_CHLTR (P17821) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70) (75 kDa membrane
protein)
Length = 659
Score = 38.5 bits (88), Expect = 0.003
Identities = 22/69 (31%), Positives = 35/69 (49%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ + L+K+K + E + + + V TVP F +R + A A LD ++P
Sbjct: 113 TPEEIGAQILMKMKETAEAYLGETVTEAVITVPAYFNDSQRASTKDAGRIAGLDVKRIIP 172
Query: 84 QPIAVALFY 92
+P A AL Y
Sbjct: 173 EPTAAALAY 181
>DNAK_CHLPN (P27542) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70) (75 kDa membrane
protein)
Length = 660
Score = 38.5 bits (88), Expect = 0.003
Identities = 22/69 (31%), Positives = 35/69 (49%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ + L+K+K + E + + + V TVP F +R + A A LD ++P
Sbjct: 114 TPEEIGAQILMKMKETAEAYLGETVTEAVITVPAYFNDSQRASTKDAGRIAGLDVKRIIP 173
Query: 84 QPIAVALFY 92
+P A AL Y
Sbjct: 174 EPTAAALAY 182
>DNAK_CHLMU (P56836) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70) (75 kDa membrane
protein)
Length = 654
Score = 38.5 bits (88), Expect = 0.003
Identities = 22/69 (31%), Positives = 35/69 (49%)
Query: 24 TAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVELMP 83
T E+ + L+K+K + E + + + V TVP F +R + A A LD ++P
Sbjct: 113 TPEEIGAQILMKMKETAEAYLGETVTEAVITVPAYFNDSQRASTKDAGRIAGLDVKRIIP 172
Query: 84 QPIAVALFY 92
+P A AL Y
Sbjct: 173 EPTAAALAY 181
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.134 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,096,467
Number of Sequences: 164201
Number of extensions: 312469
Number of successful extensions: 1046
Number of sequences better than 10.0: 295
Number of HSP's better than 10.0 without gapping: 289
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 757
Number of HSP's gapped (non-prelim): 295
length of query: 109
length of database: 59,974,054
effective HSP length: 85
effective length of query: 24
effective length of database: 46,016,969
effective search space: 1104407256
effective search space used: 1104407256
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135505.7