BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.9 + phase: 0 /pseudo
(1303 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 50 4e-05
GLH2_CAEEL (Q966L9) ATP-dependent RNA helicase glh-2 (EC 3.6.1.-... 43 0.006
GLH1_CAEEL (P34689) ATP-dependent RNA helicase glh-1 (EC 3.6.1.-... 41 0.019
GAG_EIAVY (P03351) Gag polyprotein [Contains: Core protein p15; ... 41 0.019
CNBP_RAT (P62634) Cellular nucleic acid binding protein (CNBP) (... 39 0.071
CNBP_MOUSE (P53996) Cellular nucleic acid binding protein (CNBP)... 39 0.071
CNBP_HUMAN (P62633) Cellular nucleic acid binding protein (CNBP)... 39 0.071
CNBP_CHICK (O42395) Cellular nucleic acid binding protein (CNBP) 39 0.071
GAG_SRV2 (P51516) Gag polyprotein (Core polyprotein) [Contains: ... 39 0.12
GAG_IPMA (P11365) Retrovirus-related Gag polyprotein [Contains: ... 39 0.12
ZCH9_HUMAN (Q8N567) Zinc finger CCHC domain containing protein 9 38 0.16
ZCH9_MOUSE (Q8R1J3) Zinc finger CCHC domain containing protein 9 37 0.46
GRP2_NICSY (P27484) Glycine-rich protein 2 36 0.60
GAG_HV2RO (P04590) Gag polyprotein [Contains: Core protein p16; ... 36 0.78
GAG_HV2CA (P24106) Gag polyprotein [Contains: Core protein p16; ... 36 0.78
GAG_HV2BE (P18095) Gag polyprotein [Contains: Core protein p16; ... 36 0.78
GAG_FIVSD (P19027) Gag polyprotein [Contains: Core protein p15; ... 36 0.78
GAG_HV2D1 (P17756) Gag polyprotein [Contains: Core protein p16; ... 35 1.0
GAG_BAEVM (P03341) Gag polyprotein [Contains: Inner coat protein... 35 1.0
HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding p... 35 1.3
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 50.1 bits (118), Expect = 4e-05
Identities = 26/85 (30%), Positives = 44/85 (51%), Gaps = 7/85 (8%)
Query: 156 IRLEPFNL*YSLFFLGKGKIEKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKK 215
+R +P N +L G+G+ ++ + YG+ RG KN K + C+NC +
Sbjct: 185 MRKKPENQGQALITEGRGRSYQRSSNN-------YGRSGARGKSKNRSKSRVRNCYNCNQ 237
Query: 216 PGHFIADCPDLQKEKSKSRPKKQSE 240
PGHF DCP+ +K K ++ +K +
Sbjct: 238 PGHFKRDCPNPRKGKGETSGQKNDD 262
>GLH2_CAEEL (Q966L9) ATP-dependent RNA helicase glh-2 (EC 3.6.1.-)
(Germline helicase-2)
Length = 974
Score = 42.7 bits (99), Expect = 0.006
Identities = 20/47 (42%), Positives = 25/47 (52%), Gaps = 3/47 (6%)
Query: 184 FGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEK 230
FGG G GG R E CFNC++PGH DCP+ +KE+
Sbjct: 350 FGGN---SNGFGSGGGGQDRGERNNNCFNCQQPGHRSNDCPEPKKER 393
Score = 42.7 bits (99), Expect = 0.006
Identities = 22/56 (39%), Positives = 27/56 (47%), Gaps = 9/56 (16%)
Query: 184 FGGRFRYGKVLGRGGYKNS---------RKEDQKGCFNCKKPGHFIADCPDLQKEK 230
FGG G G GG N R E CFNC++PGH DCP+ +KE+
Sbjct: 224 FGGGNSGGSGFGSGGNSNGFGSGGGGQDRGERNNNCFNCQQPGHRSNDCPEPKKER 279
Score = 35.8 bits (81), Expect = 0.78
Identities = 21/57 (36%), Positives = 28/57 (48%), Gaps = 7/57 (12%)
Query: 176 EKKPLESK--FGGRFRYGKVLGRGG-----YKNSRKEDQKGCFNCKKPGHFIADCPD 225
E+KP E + F F G G GG + N+ + CFNCK GH A+CP+
Sbjct: 414 ERKPREGRNGFTSGFGGGNDGGFGGGNAEGFGNNEERGPMKCFNCKGEGHRSAECPE 470
Score = 33.5 bits (75), Expect = 3.9
Identities = 10/28 (35%), Positives = 20/28 (70%)
Query: 203 RKEDQKGCFNCKKPGHFIADCPDLQKEK 230
++ + + C+NC++PGH DCP+ +K +
Sbjct: 391 KEREPRVCYNCQQPGHNSRDCPEERKPR 418
Score = 33.5 bits (75), Expect = 3.9
Identities = 10/28 (35%), Positives = 20/28 (70%)
Query: 203 RKEDQKGCFNCKKPGHFIADCPDLQKEK 230
++ + + C+NC++PGH DCP+ +K +
Sbjct: 277 KEREPRVCYNCQQPGHNSRDCPEERKPR 304
>GLH1_CAEEL (P34689) ATP-dependent RNA helicase glh-1 (EC 3.6.1.-)
(Germline helicase-1)
Length = 763
Score = 41.2 bits (95), Expect = 0.019
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 3/47 (6%)
Query: 185 GGRFRYGKV-LGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEK 230
GG F G G GG+ + + CFNC++PGH +DCP+ +KE+
Sbjct: 136 GGSFGGGNSGFGEGGHGGGERNNN--CFNCQQPGHRSSDCPEPRKER 180
Score = 38.1 bits (87), Expect = 0.16
Identities = 21/59 (35%), Positives = 28/59 (46%), Gaps = 9/59 (15%)
Query: 176 EKKPLESK---FGGRFRYGKVLGR------GGYKNSRKEDQKGCFNCKKPGHFIADCPD 225
E+KP E + FGG +G G GG+ + CFNCK GH A+CP+
Sbjct: 201 ERKPREGRTGGFGGGAGFGNNGGNDGFGGDGGFGGGEERGPMKCFNCKGEGHRSAECPE 259
>GAG_EIAVY (P03351) Gag polyprotein [Contains: Core protein p15;
Major core protein p26 (Capsid protein p26); Core
protein p11; Core protein p9]
Length = 486
Score = 41.2 bits (95), Expect = 0.019
Identities = 19/48 (39%), Positives = 29/48 (59%), Gaps = 3/48 (6%)
Query: 195 GRGGYKNSRKEDQKGCFNCKKPGHFIADC---PDLQKEKSKSRPKKQS 239
G+ G+ +S+ K CF CK+PGHF C P K+ ++ RP+KQ+
Sbjct: 387 GKPGHLSSQCRAPKVCFKCKQPGHFSKQCRSVPKNGKQGAQGRPQKQT 434
>CNBP_RAT (P62634) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 177
Score = 39.3 bits (90), Expect = 0.071
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Query: 195 GRGGY-----KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKS 233
GRGG+ K ++E ++ C+NC KPGH DC ++K S
Sbjct: 78 GRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYS 121
>CNBP_MOUSE (P53996) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 178
Score = 39.3 bits (90), Expect = 0.071
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Query: 195 GRGGY-----KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKS 233
GRGG+ K ++E ++ C+NC KPGH DC ++K S
Sbjct: 79 GRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYS 122
Score = 36.2 bits (82), Expect = 0.60
Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Query: 185 GGRFRYGKVLGRGGYKNSRKEDQKG------CFNCKKPGHFIADCPDLQKEKSKSRPKKQ 238
GGR R + GRGG+ + R C+ C + GH DC DLQ++++ +
Sbjct: 23 GGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDC-DLQEDEACYNCGRG 81
Query: 239 SELVKTAGVPKGKRE 253
+ K PK +RE
Sbjct: 82 GHIAKDCKEPKRERE 96
>CNBP_HUMAN (P62633) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 177
Score = 39.3 bits (90), Expect = 0.071
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Query: 195 GRGGY-----KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKS 233
GRGG+ K ++E ++ C+NC KPGH DC ++K S
Sbjct: 78 GRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYS 121
>CNBP_CHICK (O42395) Cellular nucleic acid binding protein (CNBP)
Length = 172
Score = 39.3 bits (90), Expect = 0.071
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Query: 195 GRGGY-----KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKS 233
GRGG+ K ++E ++ C+NC KPGH DC ++K S
Sbjct: 73 GRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYS 116
Score = 36.2 bits (82), Expect = 0.60
Identities = 21/68 (30%), Positives = 32/68 (46%), Gaps = 1/68 (1%)
Query: 186 GRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRPKKQSELVKTA 245
GR R + GR G++ C+ C + GH DC DLQ++K+ + + K
Sbjct: 24 GRGRGMRSRGRAGFQFMSSSLPDICYRCGESGHLAKDC-DLQEDKACYNCGRGGHIAKDC 82
Query: 246 GVPKGKRE 253
PK +RE
Sbjct: 83 KEPKRERE 90
Score = 32.3 bits (72), Expect = 8.7
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Query: 195 GRGGY--KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSK 232
G G+ K+ ++ K C+NC + GH DC + ++E+ +
Sbjct: 52 GESGHLAKDCDLQEDKACYNCGRGGHIAKDCKEPKREREQ 91
>GAG_SRV2 (P51516) Gag polyprotein (Core polyprotein) [Contains:
Core protein p10; Core phosphoprotein p18; Core protein
p12; Core protein p27; Core protein p14; Core protein
p4]
Length = 654
Score = 38.5 bits (88), Expect = 0.12
Identities = 14/34 (41%), Positives = 21/34 (61%)
Query: 201 NSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSR 234
N++ +D+ GCF C K GHF DC D + +S+
Sbjct: 537 NNKNKDRGGCFKCGKKGHFAKDCRDHSNKNPESK 570
>GAG_IPMA (P11365) Retrovirus-related Gag polyprotein [Contains:
Protease (EC 3.4.23.-)]
Length = 827
Score = 38.5 bits (88), Expect = 0.12
Identities = 16/30 (53%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Query: 200 KNSRKEDQKGCFNCKKPGHFIADC--PDLQ 227
++ + DQ+ CFNC KPGHF DC PD Q
Sbjct: 450 RSMSRNDQRTCFNCGKPGHFKKDCRAPDKQ 479
>ZCH9_HUMAN (Q8N567) Zinc finger CCHC domain containing protein 9
Length = 271
Score = 38.1 bits (87), Expect = 0.16
Identities = 15/30 (50%), Positives = 21/30 (70%)
Query: 195 GRGGYKNSRKEDQKGCFNCKKPGHFIADCP 224
GR + + K++ CF+C+KPGH IADCP
Sbjct: 115 GRRLKRQAAKKNAMVCFHCRKPGHGIADCP 144
>ZCH9_MOUSE (Q8R1J3) Zinc finger CCHC domain containing protein 9
Length = 273
Score = 36.6 bits (83), Expect = 0.46
Identities = 14/30 (46%), Positives = 21/30 (69%)
Query: 195 GRGGYKNSRKEDQKGCFNCKKPGHFIADCP 224
GR + + K++ CF+C++PGH IADCP
Sbjct: 115 GRRLKRQAAKKNAMVCFHCRQPGHGIADCP 144
>GRP2_NICSY (P27484) Glycine-rich protein 2
Length = 214
Score = 36.2 bits (82), Expect = 0.60
Identities = 18/44 (40%), Positives = 20/44 (44%), Gaps = 4/44 (9%)
Query: 184 FGGRFRYGK----VLGRGGYKNSRKEDQKGCFNCKKPGHFIADC 223
+GG RYG G GGY GCF C + GHF DC
Sbjct: 129 YGGGSRYGGGGGGYGGGGGYGGGGSGGGSGCFKCGESGHFARDC 172
>GAG_HV2RO (P04590) Gag polyprotein [Contains: Core protein p16;
Core protein p26]
Length = 522
Score = 35.8 bits (81), Expect = 0.78
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Query: 195 GRGGY--KNSRKEDQKGCFNCKKPGHFIADCPDLQ 227
G+ G+ + R ++GC+ C KPGH + +CPD Q
Sbjct: 395 GKEGHSARQCRAPRRQGCWKCGKPGHIMTNCPDRQ 429
>GAG_HV2CA (P24106) Gag polyprotein [Contains: Core protein p16;
Core protein p26]
Length = 521
Score = 35.8 bits (81), Expect = 0.78
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Query: 195 GRGGY--KNSRKEDQKGCFNCKKPGHFIADCPDLQ 227
G+ G+ + R ++GC+ C KPGH + +CPD Q
Sbjct: 395 GKEGHSARQCRAPRRQGCWKCGKPGHIMTNCPDRQ 429
>GAG_HV2BE (P18095) Gag polyprotein [Contains: Core protein p16;
Core protein p26]
Length = 521
Score = 35.8 bits (81), Expect = 0.78
Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Query: 195 GRGGY--KNSRKEDQKGCFNCKKPGHFIADCPDLQ 227
G+ G+ + R ++GC+ C KPGH +A+CP+ Q
Sbjct: 395 GKEGHSARQCRAPRRQGCWKCGKPGHIMANCPERQ 429
>GAG_FIVSD (P19027) Gag polyprotein [Contains: Core protein p15;
Major core protein p24; Nucleic acid binding protein
p10]
Length = 450
Score = 35.8 bits (81), Expect = 0.78
Identities = 13/26 (50%), Positives = 16/26 (61%)
Query: 210 CFNCKKPGHFIADCPDLQKEKSKSRP 235
CFNCKKPGH C D++K +P
Sbjct: 377 CFNCKKPGHLARQCRDVKKCNKCGKP 402
>GAG_HV2D1 (P17756) Gag polyprotein [Contains: Core protein p16;
Core protein p26]
Length = 521
Score = 35.4 bits (80), Expect = 1.0
Identities = 20/59 (33%), Positives = 30/59 (49%), Gaps = 3/59 (5%)
Query: 195 GRGGY--KNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRPK-KQSELVKTAGVPKG 250
G+ G+ K R ++GC+ C K GH +A+CP+ Q P+ KQ A P+G
Sbjct: 395 GKEGHSAKQCRAPRRQGCWKCGKSGHIMANCPERQAGFLGMGPRGKQPRNFPAAQAPQG 453
>GAG_BAEVM (P03341) Gag polyprotein [Contains: Inner coat protein
p12; Core protein p15; Core shell protein p30;
Nucleoprotein p10]
Length = 537
Score = 35.4 bits (80), Expect = 1.0
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 1/45 (2%)
Query: 192 KVLGRGGYKNSR-KEDQKGCFNCKKPGHFIADCPDLQKEKSKSRP 235
K G+ G R K D+ C CK+ GH+I DCP +++ K P
Sbjct: 484 KRAGKSGETRRRPKVDKDQCAYCKERGHWIKDCPKRPRDQKKPAP 528
>HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding
protein)
Length = 271
Score = 35.0 bits (79), Expect = 1.3
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Query: 172 KGKIEKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQ 227
+G + + S+ G R YG+ GR G + D+ C+ C GH DCP+ Q
Sbjct: 105 EGHLSRDCPSSQGGSRGGYGQKRGRSGAQGGYSGDRT-CYKCGDAGHISRDCPNGQ 159
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.382 0.170 0.717
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,574,027
Number of Sequences: 164201
Number of extensions: 3491139
Number of successful extensions: 20138
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 20000
Number of HSP's gapped (non-prelim): 167
length of query: 1303
length of database: 59,974,054
effective HSP length: 122
effective length of query: 1181
effective length of database: 39,941,532
effective search space: 47170949292
effective search space used: 47170949292
T: 11
A: 40
X1: 13 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 35 (21.8 bits)
S2: 72 (32.3 bits)
Medicago: description of AC135504.9