BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.2 - phase: 0 /pseudo
(1353 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 84 3e-15
M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810... 83 4e-15
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 80 3e-14
M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240... 54 4e-06
YJZ7_YEAST (P47098) Transposon Ty1 protein B 45 0.002
YMD9_YEAST (Q03434) Transposon Ty1 protein B 44 0.004
YME4_YEAST (Q04711) Transposon Ty1 protein B 40 0.033
YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B) 39 0.074
YMT5_YEAST (Q04214) Transposon Ty1 protein B 39 0.13
YJZ9_YEAST (P47100) Transposon Ty1 protein B 39 0.13
YMU0_YEAST (Q04670) Transposon Ty1 protein B 38 0.16
YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein 33 5.3
NU4M_CERCA (Q34048) NADH-ubiquinone oxidoreductase chain 4 (EC 1... 33 6.9
COX3_LEITA (P14546) Cytochrome c oxidase polypeptide III (EC 1.9... 33 6.9
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 84.0 bits (206), Expect = 3e-15
Identities = 43/106 (40%), Positives = 63/106 (58%), Gaps = 1/106 (0%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCF-FIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCE 1253
++GY D+DW G R+S TGY F +L+ W +K+Q V+ SS+EAEY AL + E
Sbjct: 1248 IIGYVDSDWAGSEIDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVRE 1307
Query: 1254 LQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
L +LLT +N+ + D+Q + IA+NP H+R KH+DI
Sbjct: 1308 ALWLKFLLTSINIKLENPIKIYEDNQGCISIANNPSCHKRAKHIDI 1353
>M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810
(ORF240b)
Length = 240
Score = 83.2 bits (204), Expect = 4e-15
Identities = 35/71 (49%), Positives = 51/71 (71%)
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
GL+ ++S L++ + D+DW GC TRRS TG+C F+G +++SW +K+Q VSRSS+E E
Sbjct: 153 GLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETE 212
Query: 1244 YRALVASTCEL 1254
YRAL + EL
Sbjct: 213 YRALALTAAEL 223
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 80.5 bits (197), Expect = 3e-14
Identities = 44/105 (41%), Positives = 64/105 (60%), Gaps = 1/105 (0%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L GY+DAD G D R+S TGY F +SW+SK Q+ V+ S++EAEY A + E+
Sbjct: 1175 LKGYTDADMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEM 1234
Query: 1255 Q*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
L L +L + K + CDSQSA+ ++ N ++H RTKH+D+
Sbjct: 1235 IWLKRFLQELGLH-QKEYVVYCDSQSAIDLSKNSMYHARTKHIDV 1278
>M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240
(ORF111a)
Length = 111
Score = 53.5 bits (127), Expect = 4e-06
Identities = 20/39 (51%), Positives = 27/39 (68%)
Query: 1182 GTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFI 1220
G GLF+ S L L ++D+DW CPDTRRS+TG+C +
Sbjct: 44 GQGLFYSATSDLQLKAFADSDWASCPDTRRSVTGFCSLV 82
>YJZ7_YEAST (P47098) Transposon Ty1 protein B
Length = 1755
Score = 44.7 bits (104), Expect = 0.002
Identities = 32/97 (32%), Positives = 48/97 (48%), Gaps = 2/97 (2%)
Query: 1188 PRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRAL 1247
P + L+ SDA +G P + I G F + ++ KS K + S++EAE A+
Sbjct: 1598 PTEPDNKLVAISDASYGNQPYYKSQI-GNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAI 1656
Query: 1248 VASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHI 1284
S L L YL+ +LN + GLL DS+S + I
Sbjct: 1657 SESVPLLNNLSYLIQELNKK-PIIKGLLTDSRSTISI 1692
>YMD9_YEAST (Q03434) Transposon Ty1 protein B
Length = 1328
Score = 43.5 bits (101), Expect = 0.004
Identities = 31/97 (31%), Positives = 48/97 (48%), Gaps = 2/97 (2%)
Query: 1188 PRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRAL 1247
P + L+ SDA +G P + I G + + ++ KS K + S++EAE A+
Sbjct: 1171 PTEPDNKLVAISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAI 1229
Query: 1248 VASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHI 1284
S L L YL+ +LN + GLL DS+S + I
Sbjct: 1230 SESVPLLNNLSYLIQELNKK-PIIKGLLTDSRSTISI 1265
>YME4_YEAST (Q04711) Transposon Ty1 protein B
Length = 1328
Score = 40.4 bits (93), Expect = 0.033
Identities = 32/100 (32%), Positives = 49/100 (49%), Gaps = 8/100 (8%)
Query: 1188 PRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRAL 1247
P + L+ SDA +G P + I G + + ++ KS K + S++EAE A+
Sbjct: 1171 PTEPDNKLVAISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAI 1229
Query: 1248 VASTCELQ*LVYLLTDLNVTCSKVP---GLLCDSQSALHI 1284
S L L +L+ +LN K P GLL DS+S + I
Sbjct: 1230 SESVPLLNNLSHLVQELN----KKPITKGLLTDSKSTISI 1265
>YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B)
Length = 1770
Score = 39.3 bits (90), Expect = 0.074
Identities = 29/90 (32%), Positives = 46/90 (50%), Gaps = 2/90 (2%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L+ SDA +G P + I G F + ++ KS K + S++EAE A+ + L
Sbjct: 1620 LVAISDASYGNQPYYKSQI-GNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVSEAIPLL 1678
Query: 1255 Q*LVYLLTDLNVTCSKVPGLLCDSQSALHI 1284
L +L+ +LN + GLL DS+S + I
Sbjct: 1679 NNLSHLVQELNKK-PIIKGLLTDSRSTISI 1707
>YMT5_YEAST (Q04214) Transposon Ty1 protein B
Length = 1328
Score = 38.5 bits (88), Expect = 0.13
Identities = 31/93 (33%), Positives = 47/93 (50%), Gaps = 8/93 (8%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L+ SDA +G P + I G + + ++ KS K + S++EAE A+ S L
Sbjct: 1178 LVVISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLL 1236
Query: 1255 Q*LVYLLTDLNVTCSKVP---GLLCDSQSALHI 1284
L YL+ +L+ K P GLL DS+S + I
Sbjct: 1237 NNLSYLIQELD----KKPITKGLLTDSKSTISI 1265
>YJZ9_YEAST (P47100) Transposon Ty1 protein B
Length = 1755
Score = 38.5 bits (88), Expect = 0.13
Identities = 31/93 (33%), Positives = 47/93 (50%), Gaps = 8/93 (8%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L+ SDA +G P + I G + + ++ KS K + S++EAE A+ S L
Sbjct: 1605 LVVISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLL 1663
Query: 1255 Q*LVYLLTDLNVTCSKVP---GLLCDSQSALHI 1284
L YL+ +L+ K P GLL DS+S + I
Sbjct: 1664 NNLSYLIQELD----KKPITKGLLTDSKSTISI 1692
>YMU0_YEAST (Q04670) Transposon Ty1 protein B
Length = 1328
Score = 38.1 bits (87), Expect = 0.16
Identities = 31/93 (33%), Positives = 47/93 (50%), Gaps = 8/93 (8%)
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L+ SDA +G P + I G + + ++ KS K + S++EAE A+ S L
Sbjct: 1178 LVVISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLL 1236
Query: 1255 Q*LVYLLTDLNVTCSKVP---GLLCDSQSALHI 1284
L +L+ +LN K P GLL DS+S + I
Sbjct: 1237 NNLSHLVQELN----KKPITKGLLTDSKSTISI 1265
>YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein
Length = 308
Score = 33.1 bits (74), Expect = 5.3
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Query: 1191 SSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKK-QQIVSRSSSEAEY 1244
S + L Y DA G D S GY + + V+W SKK + ++ S+EAEY
Sbjct: 244 SQVALTVYCDASHGAIHDLPHSTGGYVTLLAGAPVTWSSKKLKGVIPVPSTEAEY 298
>NU4M_CERCA (Q34048) NADH-ubiquinone oxidoreductase chain 4 (EC
1.6.5.3)
Length = 446
Score = 32.7 bits (73), Expect = 6.9
Identities = 54/228 (23%), Positives = 93/228 (40%), Gaps = 14/228 (6%)
Query: 635 IKYLLMLFKEKFLINLCTMNCLICLVSKYLFHSALLLLKIVINLNWTIELANVF-FSVLD 693
+K +LM+ F+ +C M L +V LF +L+ + LN+ + ++ F VL
Sbjct: 2 LKLILMMI---FISPICFMQGLFWMVHNLLFVITFVLILMNFCLNYFVNISYFMGFDVLS 58
Query: 694 KG*KTMLF--*TYIISQSLCLDTF-NLKKLFFHTNLYLLLNLAGSTSHFPSHHIPIHHYQ 750
G + F + +I S ++ + N K LF L LL+ L + S +
Sbjct: 59 YGLILLSFWICSLMIVASESVNKYNNYKNLFLFNVLMLLIFLVLTFSSMNLFMFYLFFES 118
Query: 751 HS*PHLLLT----YQPQITHHTLLHLHLSLTLTLPLNQLCLPP*ENPPDQKDYPSILWTI 806
P L L YQP+ + L +L ++LPL +N Y +
Sbjct: 119 SLIPTLFLILGWGYQPERLQAGVYLLFYTLLVSLPLLVGIFYLYKNCNTMNFYLLSNYMF 178
Query: 807 NVTKLFIRHLIPLLTLLAIPIFHLFSTSTHFLSSQSMSPL---AMLKL 851
N L++ ++ L + + + HL+ H + S S + MLKL
Sbjct: 179 NCNLLYLAMILAFLVKMPMFLVHLWLPKAHVEAPVSGSMILAGIMLKL 226
>COX3_LEITA (P14546) Cytochrome c oxidase polypeptide III (EC
1.9.3.1)
Length = 284
Score = 32.7 bits (73), Expect = 6.9
Identities = 19/71 (26%), Positives = 37/71 (51%), Gaps = 2/71 (2%)
Query: 624 GLWSLMLFLL*IKYLLMLFKEKFLINLCTMNC--LICLVSKYLFHSALLLLKIVINLNWT 681
G++ +LFL+ + + +F FL+ LC C +C+ ++++F + L ++ W
Sbjct: 207 GVFGSILFLIDLLHFSHVFLGIFLLFLCFSRCFNFLCMDTRFVFLYVVCLYWHFVDCVWF 266
Query: 682 IELANVFFSVL 692
L V+F VL
Sbjct: 267 FLLRFVYFDVL 277
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.360 0.159 0.618
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 132,092,676
Number of Sequences: 164201
Number of extensions: 4767284
Number of successful extensions: 18906
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 18891
Number of HSP's gapped (non-prelim): 23
length of query: 1353
length of database: 59,974,054
effective HSP length: 122
effective length of query: 1231
effective length of database: 39,941,532
effective search space: 49168025892
effective search space used: 49168025892
T: 11
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 72 (32.3 bits)
Medicago: description of AC135464.2