BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135416.15 - phase: 0 /pseudo
(662 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LMA1_MOUSE (P19137) Laminin alpha-1 chain precursor (Laminin A c... 35 0.82
VSAC_TRYBB (P06016) Variant surface glycoprotein ANTAT 1.1C prec... 34 1.1
EF1D_XENLA (P29693) Elongation factor 1-delta (EF-1-delta) (P36) 34 1.1
HPPD_PIG (Q02110) 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.1... 34 1.4
GATB_SULSO (Q980Q3) Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransfe... 34 1.4
FA8_PIG (P12263) Coagulation factor VIII precursor (Procoagulant... 33 1.8
K2C5_XENLA (P16878) Keratin, type II cytoskeletal (XENCK55(5/6)) 33 2.4
NRP3_HUMAN (Q9NQ35) Nuclear receptor interacting protein 3 (Sarc... 33 3.1
CCA_SCHMA (O02197) Circulating cathodic antigen (CCA) 33 3.1
K2C1_HUMAN (P04264) Keratin, type II cytoskeletal 1 (Cytokeratin... 32 4.1
GIDA_XYLFA (Q9PBN4) Glucose inhibited division protein A 32 4.1
DPD2_CAEEL (Q19366) Preobable DNA polymerase delta small subunit... 32 4.1
AN18_HUMAN (Q8IVF6) Ankyrin repeat domain protein 18A 32 4.1
NRP3_MOUSE (Q9JJR9) Nuclear receptor interacting protein 3 32 5.3
LEU3_LISMO (Q8Y5R8) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 32 5.3
YLP3_CAEEL (P34383) Hypothetical protein F02A9.3 in chromosome III 32 7.0
PRPU_MOUSE (Q91YR7) U5 snRNP-associated 102 kDa protein (U5-102 ... 32 7.0
MLP2_YEAST (P40457) MLP2 protein (Myosin-like protein 2) 32 7.0
HPPD_HUMAN (P32754) 4-hydroxyphenylpyruvate dioxygenase (EC 1.13... 32 7.0
VP15_YEAST (P22219) Protein kinase VPS15 (EC 2.7.1.-) 31 9.1
>LMA1_MOUSE (P19137) Laminin alpha-1 chain precursor (Laminin A chain)
Length = 3084
Score = 34.7 bits (78), Expect = 0.82
Identities = 26/75 (34%), Positives = 38/75 (50%), Gaps = 9/75 (12%)
Query: 290 AKKSSLEILLENCIANQNKNLQELKNQTGFL------NGSLSKLTTKVDSIATHTKMLKT 343
AKK EI LE IA Q +NLQ K T L N + + + ++AT + L
Sbjct: 1617 AKKIRAEIQLEG-IAEQTENLQ--KELTRVLARHQKVNAEMERTSNGTQALATFIEQLHA 1673
Query: 344 QISQVAQQVATSSQT 358
I ++ ++VAT +QT
Sbjct: 1674 NIKEITEKVATLNQT 1688
>VSAC_TRYBB (P06016) Variant surface glycoprotein ANTAT 1.1C
precursor (VSG) (Fragment)
Length = 482
Score = 34.3 bits (77), Expect = 1.1
Identities = 30/113 (26%), Positives = 54/113 (47%), Gaps = 8/113 (7%)
Query: 304 ANQNKNLQELKNQTGFLNGSLSKLTTKVDSIATHTKMLKTQISQVAQQV---ATSSQTPG 360
A NK + LKN+ G +KL T + T + LKT ++ ++ A + +
Sbjct: 230 APTNKLISALKNKGA---GVAAKLATVTSAAPTSKQELKTLLASKGERAKLQAANDEYNN 286
Query: 361 VFPGQPETNPKAHVNAIFLGGSKLEETVAKAKSVKGESVKCLGKKGVIESEKL 413
PG + AH+ +F G++ + A A +++G S++ K G ES++L
Sbjct: 287 WKPGAKPEDFDAHIKKVF--GAEDGKDSAYALALEGISIEVPQKPGTTESKQL 337
>EF1D_XENLA (P29693) Elongation factor 1-delta (EF-1-delta) (P36)
Length = 265
Score = 34.3 bits (77), Expect = 1.1
Identities = 44/175 (25%), Positives = 78/175 (44%), Gaps = 23/175 (13%)
Query: 250 EQLNYAQFNQGTRPNQKFYKNPQGSYGQVAPPGYTNNQRVAKKSSLEILLENCIANQNKN 309
E+ Y + G+ N K + +PQ + ++ G ++ A+ ++LE +N++
Sbjct: 22 EKQYYENLSMGSASN-KPHNSPQSAASALSNSG-DGSELAARVANLE--------QENQS 71
Query: 310 LQELKNQTGFLNGSLSKLTTKVDSIATHTKMLK-TQISQVAQQVATSSQTPGVFPGQPET 368
L ++ L ++SKL +++ ++ +K K SQ A +VA Q V P E
Sbjct: 72 LHKVVKD---LQSAISKLESRLSTLEKSSKSQKPAAASQPAIEVAARVQKVQVTPAAKEE 128
Query: 369 NPKAH----VNAIFLGGSKLEETVAKAKSVKGESVKCLGKK-----GVIESEKLL 414
N + I L GS EE A+A ++ E +K +K GVI +L
Sbjct: 129 NGTGEDDDDDDDIDLFGSDNEEEDAEAARIREERLKQYAEKKSKKPGVIAKSSIL 183
>HPPD_PIG (Q02110) 4-hydroxyphenylpyruvate dioxygenase (EC
1.13.11.27) (4HPPD) (HPD) (HPPDase)
Length = 392
Score = 33.9 bits (76), Expect = 1.4
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 7/70 (10%)
Query: 156 HTEAYSLIEDMAQNHYQWTNERAVTAPTPSKKEAGIYEVSEYN------HLAAKVEALTQ 209
HTE YS + + +Y+ + + + P P KK++ I E +YN H+A K E +
Sbjct: 217 HTE-YSALRSVVMANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIIT 275
Query: 210 KFEKLNVNAV 219
L V
Sbjct: 276 AIRSLRERGV 285
>GATB_SULSO (Q980Q3) Aspartyl/glutamyl-tRNA(Asn/Gln)
amidotransferase subunit B (EC 6.3.5.-) (Asp/Glu-ADT
subunit B)
Length = 472
Score = 33.9 bits (76), Expect = 1.4
Identities = 33/136 (24%), Positives = 56/136 (40%), Gaps = 14/136 (10%)
Query: 68 WDDMRRAFLARFFPPSKTAKLRDQITRFNQKD---GEYLYEAWERFKEMLRLCPHHGLEK 124
WD+ RR + P +T + + F D E E+FK + P E+
Sbjct: 246 WDNERRVTV-----PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAER 300
Query: 125 WLIVHTFYNGLSYTTKMSVDAAACGALMNK--NHTEAYSLIEDMAQNHY-QWTNERAVTA 181
++ YN Y ++ V+ A L + N+ + Y+ + ++ N Y +W NE +T
Sbjct: 301 FV---KQYNVTPYQAQVLVNEKALADLFEEITNNYKNYAKVANLLINDYMRWLNENNITV 357
Query: 182 PTPSKKEAGIYEVSEY 197
K I E+ EY
Sbjct: 358 TQSKAKANHIIELFEY 373
>FA8_PIG (P12263) Coagulation factor VIII precursor (Procoagulant
component)
Length = 2133
Score = 33.5 bits (75), Expect = 1.8
Identities = 32/113 (28%), Positives = 50/113 (43%), Gaps = 17/113 (15%)
Query: 324 LSKLTTKVDSIATHTKMLKTQISQVAQQVATSSQTPGVFPGQPETNPKAHVNAIFLG--- 380
L KL +K+ S + K T S A + +T + P P+ N ++ + AI LG
Sbjct: 873 LKKLDSKMSSSSDLLKTSPTIPSDTLS--AETERTHSLGPPHPQVNFRSQLGAIVLGKNS 930
Query: 381 ------GSKLEETVAKAKSVKGESVKCLGKKGVIESEKLLDKDKALTPLRLTK 427
G L T +S GE+V +ES+ + +K++A P LTK
Sbjct: 931 SHFIGAGVPLGSTEEDHESSLGENVS------PVESDGIFEKERAHGPASLTK 977
>K2C5_XENLA (P16878) Keratin, type II cytoskeletal (XENCK55(5/6))
Length = 512
Score = 33.1 bits (74), Expect = 2.4
Identities = 18/47 (38%), Positives = 25/47 (52%)
Query: 286 NQRVAKKSSLEILLENCIANQNKNLQELKNQTGFLNGSLSKLTTKVD 332
NQ+ AK S++ L E IAN + L+ L N G L G L + V+
Sbjct: 164 NQKSAKASNIGPLFEAYIANLRRQLESLTNDKGKLEGELRNMQDLVE 210
>NRP3_HUMAN (Q9NQ35) Nuclear receptor interacting protein 3 (Sarcoma
antigen NY-SAR-105)
Length = 241
Score = 32.7 bits (73), Expect = 3.1
Identities = 34/145 (23%), Positives = 64/145 (43%), Gaps = 10/145 (6%)
Query: 477 NETKALTRENSAIIKKPPTKLRDPGSFAIPCMIGSETLNQALCDLGASVSLLPLPLF*RL 536
++T L + S +KK + + C + + +AL D G +L+ L RL
Sbjct: 86 SKTNKLNQAKSEGLKKS----EEDDMILVSCQCAGKDV-KALVDTGCLYNLISLACVDRL 140
Query: 537 GLGELKLTETTL--KLADRSDIQPVGNVEDIPVKIEGIDIPTDFMVLDIDEDNECPIILG 594
GL E + KL+ ++ VG +E + + + + + V+D DNE + LG
Sbjct: 141 GLKEHVKSHKHEGEKLSLPRHLKVVGQIEHLVITLGSLRLDCPAAVVD---DNEKNLSLG 197
Query: 595 RPFLATAGAIVDVQNGRIIFQVSDE 619
L + I+++ R+I +D+
Sbjct: 198 LQTLRSLKCIINLDKHRLIMGKTDK 222
>CCA_SCHMA (O02197) Circulating cathodic antigen (CCA)
Length = 347
Score = 32.7 bits (73), Expect = 3.1
Identities = 26/114 (22%), Positives = 48/114 (41%), Gaps = 19/114 (16%)
Query: 302 CIANQNKNLQELKNQTGFLNGSLSKLTTKVDSIATH-----TKMLKT-QISQVAQQVATS 355
C+A +NK L++LK + L SL + T +D I T +K+++T Q+ + Q V
Sbjct: 77 CLAAENKQLEQLKIENKTLRNSLDEHQTALDMIMTKYRGQISKLMRTYQVEHLVQSVI-- 134
Query: 356 SQTPGVFPGQPETNPKAHVNAIFLGGSKLEETVAKAKSVKGESVKCLGKKGVIE 409
P+ N + + + G + S+ +SV G ++
Sbjct: 135 ---------NPDNNENRCIKSFYPGPKSV--NTPSTNSIDSQSVSQKSNSGKVD 177
>K2C1_HUMAN (P04264) Keratin, type II cytoskeletal 1 (Cytokeratin 1)
(K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Length = 643
Score = 32.3 bits (72), Expect = 4.1
Identities = 28/99 (28%), Positives = 48/99 (48%), Gaps = 18/99 (18%)
Query: 273 GSYGQVAPPG----YTNNQRVAKKSSLEILLENCIANQNKNLQELKN----QTGFLNGSL 324
G YG V PPG T NQ + + ++EI + +Q++K+ Q LN
Sbjct: 140 GGYGPVCPPGGIQEVTINQSLLQPLNVEI---------DPEIQKVKSREREQIKSLNNQF 190
Query: 325 SKLTTKVDSIATHTKMLKTQISQVAQQVATSSQTPGVFP 363
+ KV + ++L+T+ ++ QQV TS++T + P
Sbjct: 191 ASFIDKVRFLEQQNQVLQTK-WELLQQVDTSTRTHNLEP 228
>GIDA_XYLFA (Q9PBN4) Glucose inhibited division protein A
Length = 629
Score = 32.3 bits (72), Expect = 4.1
Identities = 18/74 (24%), Positives = 34/74 (45%)
Query: 151 LMNKNHTEAYSLIEDMAQNHYQWTNERAVTAPTPSKKEAGIYEVSEYNHLAAKVEALTQK 210
L+ E Y + A+ Q + A T ++ G+ + + + H AK EA+ ++
Sbjct: 418 LITHGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLIDDARWAHFNAKQEAVARE 477
Query: 211 FEKLNVNAVTPSSA 224
E+L+ TP +A
Sbjct: 478 CERLSAFWATPGNA 491
>DPD2_CAEEL (Q19366) Preobable DNA polymerase delta small subunit
(EC 2.7.7.7)
Length = 451
Score = 32.3 bits (72), Expect = 4.1
Identities = 17/61 (27%), Positives = 33/61 (53%), Gaps = 6/61 (9%)
Query: 554 SDIQPVGNVEDIPVKIEGIDIPTDFMVLDIDEDNECP-----IILGRPFLATAGAIVDVQ 608
S + G++ED + I G++ D+M + + +N+CP +++G P + T DVQ
Sbjct: 190 SGLDLTGDLEDDRLTISGLEFMADWMNVQVGNENQCPPIDRLVVIG-PLVETKSNGCDVQ 248
Query: 609 N 609
+
Sbjct: 249 S 249
>AN18_HUMAN (Q8IVF6) Ankyrin repeat domain protein 18A
Length = 992
Score = 32.3 bits (72), Expect = 4.1
Identities = 35/128 (27%), Positives = 57/128 (44%), Gaps = 15/128 (11%)
Query: 383 KLEETVAKAKSVKGESVKCLGKKGVIESEKL-----------LDKDKALTPLRLTKLNLV 431
K + VA+ ++V E V V+ E L L+KDK + L LNL
Sbjct: 749 KFNDLVAEKEAVSSECVNLAKDNEVLHQELLSMRNVQEKCEKLEKDKKM--LEEEVLNLK 806
Query: 432 TQFTKFLNILQKICIKIPFAEALSRMPLYAKFLKEIFSKKKAIDHNETKALTRENSAIIK 491
T K + L K+ + +E R + L+EI +K+A + + L ++N+A +K
Sbjct: 807 THMEKDMVELGKL--QEYKSELDERAVQEIEKLEEIHLQKQAEYEKQLEQLNKDNTASLK 864
Query: 492 KPPTKLRD 499
K L+D
Sbjct: 865 KKELTLKD 872
>NRP3_MOUSE (Q9JJR9) Nuclear receptor interacting protein 3
Length = 240
Score = 32.0 bits (71), Expect = 5.3
Identities = 29/126 (23%), Positives = 55/126 (43%), Gaps = 6/126 (4%)
Query: 496 KLRDPGSFAIPCMIGSETLNQALCDLGASVSLLPLPLF*RLGLGELKLTETTL--KLADR 553
K D + C + + +AL D G +L+ RLGL + + KL+
Sbjct: 100 KCEDEDMILVSCQCAGKDV-KALVDTGCQYNLISSACVDRLGLKDHVKSHKHEGEKLSLP 158
Query: 554 SDIQPVGNVEDIPVKIEGIDIPTDFMVLDIDEDNECPIILGRPFLATAGAIVDVQNGRII 613
++ VG +E + + + + + V+D DNE + LG L + I+++ R+I
Sbjct: 159 RHLKVVGQIEHLMITVGSLRLDCQAAVVD---DNEKSLSLGLQTLRSLKCIINLDKHRLI 215
Query: 614 FQVSDE 619
+D+
Sbjct: 216 VGKTDK 221
>LEU3_LISMO (Q8Y5R8) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 350
Score = 32.0 bits (71), Expect = 5.3
Identities = 24/91 (26%), Positives = 44/91 (47%), Gaps = 4/91 (4%)
Query: 330 KVDSIATHTKMLKTQISQVAQQVATSSQTPGVFPGQPE-TNPKAHVNAIFLGGSKLEETV 388
+V S TH LK +I + V T G++ G+P+ + A V+++ +++E +
Sbjct: 107 QVPSSITHLSPLKKEIVENTDFVVVRELTGGLYFGEPKHWDESAAVDSLTYTRAEIERII 166
Query: 389 AKA---KSVKGESVKCLGKKGVIESEKLLDK 416
KA + + + V + K V+ S KL K
Sbjct: 167 EKAFEIAATRNKKVTSVDKANVLASSKLWRK 197
>YLP3_CAEEL (P34383) Hypothetical protein F02A9.3 in chromosome III
Length = 182
Score = 31.6 bits (70), Expect = 7.0
Identities = 22/62 (35%), Positives = 33/62 (52%), Gaps = 8/62 (12%)
Query: 158 EAYSLIEDMAQNHYQWTNERAVTAPTPSKKEAGIYEVSEYNHLAAKVEA-LTQKFEKLNV 216
E + ++++AQNH ++ E A +K +YE A K+EA LT KFEKL+
Sbjct: 52 EEKAALKELAQNHKEYKTEEEFKAAL-KEKSPSLYE------KAGKLEALLTAKFEKLDA 104
Query: 217 NA 218
A
Sbjct: 105 TA 106
>PRPU_MOUSE (Q91YR7) U5 snRNP-associated 102 kDa protein (U5-102 kDa
protein)
Length = 941
Score = 31.6 bits (70), Expect = 7.0
Identities = 28/123 (22%), Positives = 56/123 (44%), Gaps = 9/123 (7%)
Query: 22 IFLPSLRLSGAIKENQEAVRLHLFPFSLRDRARAWFHSLEV----GSITSWDDMRRAFLA 77
I+L +++L E + A RL S AR + S+++ G+I++ ++ L
Sbjct: 644 IWLAAVKLESENNEYERARRLLAKARSSAPTARVFMKSVKLEWVLGNISAAQELCEEALR 703
Query: 78 RFFPPSKTAKLRDQITRFNQKDGEYLYEAWERFKEMLRLCPHHGLEKWLIVHTFYNGLSY 137
+ K ++ QI ++ GE + +A E + + L+ CP H WL++ +
Sbjct: 704 HYEDFPKLWMMKGQI----EEQGELMEKAREAYNQGLKKCP-HSTPLWLLLSRLEEKIGQ 758
Query: 138 TTK 140
T+
Sbjct: 759 LTR 761
>MLP2_YEAST (P40457) MLP2 protein (Myosin-like protein 2)
Length = 1679
Score = 31.6 bits (70), Expect = 7.0
Identities = 21/96 (21%), Positives = 45/96 (46%), Gaps = 6/96 (6%)
Query: 84 KTAKLRDQITRFNQKDGEYLYEAWERFKEMLRLCPHHGLEKWLIVHTFYNGLS-YTTKMS 142
++ KLR+++ R + + E E+ +++LC + ++ LS ++
Sbjct: 968 QSEKLRNEVERIQK-----MIEKIEKMSTIVQLCKKKEMSQYQSTMKENKDLSELVIRLE 1022
Query: 143 VDAAACGALMNKNHTEAYSLIEDMAQNHYQWTNERA 178
DAA C A + K + YS + + ++ +W E+A
Sbjct: 1023 KDAADCQAELTKTKSSLYSAQDLLDKHERKWMEEKA 1058
>HPPD_HUMAN (P32754) 4-hydroxyphenylpyruvate dioxygenase (EC
1.13.11.27) (4HPPD) (HPD) (HPPDase)
Length = 392
Score = 31.6 bits (70), Expect = 7.0
Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 7/56 (12%)
Query: 156 HTEAYSLIEDMAQNHYQWTNERAVTAPTPSKKEAGIYEVSEYN------HLAAKVE 205
HTE YS + + +Y+ + + + P P KK++ I E +YN H+A K E
Sbjct: 217 HTE-YSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTE 271
>VP15_YEAST (P22219) Protein kinase VPS15 (EC 2.7.1.-)
Length = 1453
Score = 31.2 bits (69), Expect = 9.1
Identities = 21/56 (37%), Positives = 31/56 (54%), Gaps = 2/56 (3%)
Query: 397 ESVKCLGKKGVIESEKLLDKDKALTPLRLTKLNLVTQFTKFLNILQKICIKIPFAE 452
+S+K L K G+I + +D K +PL L N + QFT L I+ +I K+ AE
Sbjct: 679 QSLKSLFKTGLIRKKYYIDISKTTSPLLLHPNNWIRQFT--LMIIIEIINKLSKAE 732
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,495,082
Number of Sequences: 164201
Number of extensions: 3133359
Number of successful extensions: 8950
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 8939
Number of HSP's gapped (non-prelim): 37
length of query: 662
length of database: 59,974,054
effective HSP length: 117
effective length of query: 545
effective length of database: 40,762,537
effective search space: 22215582665
effective search space used: 22215582665
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 69 (31.2 bits)
Medicago: description of AC135416.15