BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135320.11 + phase: 0
(165 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HS2C_CHLRE (P12811) Chloroplast heat shock 22 kDa protein 34 0.17
RPOD_PICTO (Q6KZP5) DNA-directed RNA polymerase subunit D (EC 2.... 32 0.50
HS12_ORYSA (P31673) 17.4 kDa class I heat shock protein 32 0.65
HSCB_YERPE (Q8ZCS4) Co-chaperone protein hscB (Hsc20) 30 1.9
HS15_SOYBN (P04795) 17.6 kDa class I heat shock protein (HSP 17.... 30 1.9
VATE_MOUSE (P50518) Vacuolar ATP synthase subunit E (EC 3.6.3.14... 30 2.5
HS13_ARATH (P19037) 18.2 kDa class I heat shock protein (HSP 18.2) 30 2.5
HS11_ARATH (P19036) 17.4 kDa class I heat shock protein (HSP 17.4) 30 2.5
HS11_WHEAT (P12810) 16.9 kDa class I heat shock protein (Low mol... 30 3.2
TGL_BACHD (Q9K5W7) Protein-glutamine gamma-glutamyltransferase (... 29 4.2
HSPS_METJA (Q57733) Small heat shock protein HSP16.5 29 4.2
HS16_SOYBN (P05478) 18.5 kDa class I heat shock protein (HSP 18.5) 29 4.2
HS13_SOYBN (P04793) 17.5 kDa class I heat shock protein (HSP 17.... 29 4.2
HS11_ORYSA (P27777) 16.9 kDa class I heat shock protein 29 4.2
2ABD_RAT (P56932) Serine/threonine protein phosphatase 2A, 55 kD... 29 4.2
2ABD_MOUSE (Q925E7) Serine/threonine protein phosphatase 2A, 55 ... 29 4.2
YLYA_BACMT (P41024) Hypothetical 14.0 kDa protein in lysA 3'regi... 29 5.5
PRKA_BACSU (P39134) PrkA protein 29 5.5
NDF4_HUMAN (Q9HD90) Neurogenic differentiation factor 4 (NeuroD4) 29 5.5
LGUL_BRAOL (Q39366) Putative lactoylglutathione lyase (EC 4.4.1.... 29 5.5
>HS2C_CHLRE (P12811) Chloroplast heat shock 22 kDa protein
Length = 157
Score = 33.9 bits (76), Expect = 0.17
Identities = 31/108 (28%), Positives = 46/108 (41%), Gaps = 16/108 (14%)
Query: 57 ADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLDDLHPDIWR 116
A + I E PT F A+ +G + + + GV ++V L + + +WR
Sbjct: 47 APMDIIESPTAFELHADAPG-MGPDDVK-VELQEGV--LMVTGERKLSHTTKEAGGKVWR 102
Query: 117 -----------FRLPESVVPELATAVFVDGELIVTVPKAFDDENTPEP 153
F LPE+ P+ TA G L+VTVPK + PEP
Sbjct: 103 SERTAYSFSRAFSLPENANPDGITAAMDKGVLVVTVPKR-EPPAKPEP 149
>RPOD_PICTO (Q6KZP5) DNA-directed RNA polymerase subunit D (EC
2.7.7.6)
Length = 271
Score = 32.3 bits (72), Expect = 0.50
Identities = 21/55 (38%), Positives = 27/55 (48%)
Query: 29 SGFSNNNNKLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVET 83
S S N N++ V R LQ + V+I E+ T F F ETD SL +ET
Sbjct: 196 SVISENENEITFTDDVECRYLQQLFDEKSGVTITEDDTKFIFQFETDGSLTAIET 250
>HS12_ORYSA (P31673) 17.4 kDa class I heat shock protein
Length = 154
Score = 32.0 bits (71), Expect = 0.65
Identities = 16/28 (57%), Positives = 17/28 (60%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK 143
RFRLPE PE A +G L VTVPK
Sbjct: 112 RFRLPEDTKPEQIKASMENGVLTVTVPK 139
>HSCB_YERPE (Q8ZCS4) Co-chaperone protein hscB (Hsc20)
Length = 174
Score = 30.4 bits (67), Expect = 1.9
Identities = 24/76 (31%), Positives = 38/76 (49%), Gaps = 3/76 (3%)
Query: 9 ISIAYNMM-NPLSQAETLLSMSGFSNNNNKLRRLPHVFSRILQLPLRSDADVSIEEEPTC 67
I+ AY + +PL +AE +LS+ GF N + F + QL LR + D +IE +P
Sbjct: 54 INDAYQTLKHPLKRAEYMLSLQGFDLGNEQHTMRDTAF-LMEQLELREELD-AIERKPDA 111
Query: 68 FRFVAETDSSLGHVET 83
+AE + + T
Sbjct: 112 ETLLAEFSRRVAQMTT 127
>HS15_SOYBN (P04795) 17.6 kDa class I heat shock protein (HSP
17.6-L)
Length = 154
Score = 30.4 bits (67), Expect = 1.9
Identities = 16/39 (41%), Positives = 22/39 (56%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPEPI 154
RFRLPE+ E A +G L VT+PK ++ +PI
Sbjct: 112 RFRLPENAKVEQVKACMENGVLTVTIPKEEVKKSDVKPI 150
>VATE_MOUSE (P50518) Vacuolar ATP synthase subunit E (EC 3.6.3.14)
(V-ATPase E subunit) (Vacuolar proton pump E subunit)
(V-ATPase 31 kDa subunit) (P31)
Length = 228
Score = 30.0 bits (66), Expect = 2.5
Identities = 25/83 (30%), Positives = 41/83 (49%), Gaps = 22/83 (26%)
Query: 50 QLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLDD 109
++ + D DV I++EPTC R +A G VE I+ G KI + S++L LD
Sbjct: 158 KIATKRDVDVQIDQEPTCLRNIA------GGVE-----IYNGDRKI--KVSNTLESRLD- 203
Query: 110 LHPDIWRFRLPESVVPELATAVF 132
+ + ++PE+ A+F
Sbjct: 204 --------LIAQQMMPEVRGALF 218
>HS13_ARATH (P19037) 18.2 kDa class I heat shock protein (HSP 18.2)
Length = 161
Score = 30.0 bits (66), Expect = 2.5
Identities = 18/44 (40%), Positives = 23/44 (51%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPEPIGGGGA 159
RFRLPE+ E A +G L V VPKA + + + I GA
Sbjct: 117 RFRLPENAKMEEVKATMENGVLTVVVPKAPEKKPQVKSIDISGA 160
>HS11_ARATH (P19036) 17.4 kDa class I heat shock protein (HSP 17.4)
Length = 156
Score = 30.0 bits (66), Expect = 2.5
Identities = 18/37 (48%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPE 152
RFRLPE+ E A +G L VTVPK E+ PE
Sbjct: 114 RFRLPENAKVEEVKASMENGVLSVTVPKV--QESKPE 148
>HS11_WHEAT (P12810) 16.9 kDa class I heat shock protein (Low
molecular weight heat shock protein) (Heat shock protein
17) (HSP 16.9)
Length = 151
Score = 29.6 bits (65), Expect = 3.2
Identities = 16/29 (55%), Positives = 17/29 (58%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKA 144
RFRLPE E A +G L VTVPKA
Sbjct: 109 RFRLPEDAKVEEVKAGLENGVLTVTVPKA 137
>TGL_BACHD (Q9K5W7) Protein-glutamine gamma-glutamyltransferase (EC
2.3.2.13) (Transglutaminase) (TGase)
Length = 284
Score = 29.3 bits (64), Expect = 4.2
Identities = 18/55 (32%), Positives = 25/55 (44%), Gaps = 5/55 (9%)
Query: 75 DSSLGHVETHTLHIHPGVTKIVVRASHSL-----HFSLDDLHPDIWRFRLPESVV 124
DS H+ ++ H HPG+ A L +F+ D HPD FR +VV
Sbjct: 140 DSIFQHLYLYSWHTHPGLELHTFHADRFLPGDVVYFNNPDFHPDTPWFRGENAVV 194
>HSPS_METJA (Q57733) Small heat shock protein HSP16.5
Length = 147
Score = 29.3 bits (64), Expect = 4.2
Identities = 20/61 (32%), Positives = 33/61 (53%), Gaps = 1/61 (1%)
Query: 85 TLHIHPGVTKIVVRASHSLHFSLDDLHPDIWR-FRLPESVVPELATAVFVDGELIVTVPK 143
TL I + +++ S + +S +I+R +LP +V E A+A F +G L V +PK
Sbjct: 76 TLEIRAKRSPLMITESERIIYSEIPEEEEIYRTIKLPATVKEENASAKFENGVLSVILPK 135
Query: 144 A 144
A
Sbjct: 136 A 136
>HS16_SOYBN (P05478) 18.5 kDa class I heat shock protein (HSP 18.5)
Length = 161
Score = 29.3 bits (64), Expect = 4.2
Identities = 15/28 (53%), Positives = 17/28 (60%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK 143
RFRLPE+ E A +G L VTVPK
Sbjct: 119 RFRLPENAKVEQVKASMENGVLTVTVPK 146
>HS13_SOYBN (P04793) 17.5 kDa class I heat shock protein (HSP
17.5-M)
Length = 153
Score = 29.3 bits (64), Expect = 4.2
Identities = 15/28 (53%), Positives = 17/28 (60%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK 143
RFRLPE+ E A +G L VTVPK
Sbjct: 111 RFRLPENAKVEQVKASMENGVLTVTVPK 138
>HS11_ORYSA (P27777) 16.9 kDa class I heat shock protein
Length = 150
Score = 29.3 bits (64), Expect = 4.2
Identities = 15/29 (51%), Positives = 18/29 (61%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKA 144
RFRLPE+ + A +G L VTVPKA
Sbjct: 108 RFRLPENAKVDQVKAGLENGVLTVTVPKA 136
>2ABD_RAT (P56932) Serine/threonine protein phosphatase 2A, 55 kDa
regulatory subunit B, delta isoform (PP2A, subunit B,
B-delta isoform) (PP2A, subunit B, B55-delta isoform)
(PP2A, subunit B, PR55-delta isoform) (PP2A, subunit B,
R2-delta isoform)
Length = 453
Score = 29.3 bits (64), Expect = 4.2
Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 15/73 (20%)
Query: 49 LQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLD 108
L++P+ D+ +E P R A HT HI+ I V + H + S D
Sbjct: 158 LRVPILKPMDLMVEASPR--RIFANA---------HTYHIN----SISVNSDHETYLSAD 202
Query: 109 DLHPDIWRFRLPE 121
DL ++W + +
Sbjct: 203 DLRINLWHLEITD 215
>2ABD_MOUSE (Q925E7) Serine/threonine protein phosphatase 2A, 55 kDa
regulatory subunit B, delta isoform (PP2A, subunit B,
B-delta isoform) (PP2A, subunit B, B55-delta isoform)
(PP2A, subunit B, PR55-delta isoform) (PP2A, subunit B,
R2-delta isoform)
Length = 453
Score = 29.3 bits (64), Expect = 4.2
Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 15/73 (20%)
Query: 49 LQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLD 108
L++P+ D+ +E P R A HT HI+ I V + H + S D
Sbjct: 158 LRVPILKPMDLMVEASPR--RIFANA---------HTYHIN----SISVNSDHETYLSAD 202
Query: 109 DLHPDIWRFRLPE 121
DL ++W + +
Sbjct: 203 DLRINLWHLEITD 215
>YLYA_BACMT (P41024) Hypothetical 14.0 kDa protein in lysA 3'region
(ORF3)
Length = 120
Score = 28.9 bits (63), Expect = 5.5
Identities = 17/64 (26%), Positives = 31/64 (47%), Gaps = 14/64 (21%)
Query: 71 VAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLDDLHPDIWRFRLPESVVPELATA 130
V E + + H+ + H H G+ K +V+A L D++PD + ++P TA
Sbjct: 62 VNEYEVEIHHISVNPSHRHQGIGKSMVKA-------LRDIYPD-------KELIPNENTA 107
Query: 131 VFVD 134
F++
Sbjct: 108 AFIE 111
>PRKA_BACSU (P39134) PrkA protein
Length = 631
Score = 28.9 bits (63), Expect = 5.5
Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 3/54 (5%)
Query: 35 NNKLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHI 88
+NK H SRI+ +P+ + VS EEE + +AE+D + H+ HTL +
Sbjct: 304 SNKKNEALH--SRIIVMPVPYNLKVS-EEERIYEKMIAESDVADVHIAPHTLKV 354
>NDF4_HUMAN (Q9HD90) Neurogenic differentiation factor 4 (NeuroD4)
Length = 331
Score = 28.9 bits (63), Expect = 5.5
Identities = 12/26 (46%), Positives = 16/26 (61%)
Query: 79 GHVETHTLHIHPGVTKIVVRASHSLH 104
GH+ETH LH+ P V K + +S H
Sbjct: 215 GHMETHLLHLKPQVFKSLGESSFGSH 240
>LGUL_BRAOL (Q39366) Putative lactoylglutathione lyase (EC 4.4.1.5)
(Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx
I) (Ketone-aldehyde mutase) (S-D-lactoylglutathione
methylglyoxal lyase)
Length = 282
Score = 28.9 bits (63), Expect = 5.5
Identities = 29/129 (22%), Positives = 48/129 (36%), Gaps = 17/129 (13%)
Query: 39 RRLPHVFSRILQLPLRSDADVSIEEEPTCF--RFVAETDSSLGHVETHTLHIHPGVTKIV 96
RR HV R+ D D +I+ CF + + + D L P + V
Sbjct: 16 RRFLHVVYRV------GDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFV 69
Query: 97 VRASHSLHFSLDDLHPDIWRFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPEPIGG 156
V +++ S D+ F + V ++ AV G + P P+ G
Sbjct: 70 VELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVT---------REPGPVKG 120
Query: 157 GGATLVFVQ 165
GG+ + FV+
Sbjct: 121 GGSVIAFVK 129
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,230,516
Number of Sequences: 164201
Number of extensions: 788283
Number of successful extensions: 1963
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1941
Number of HSP's gapped (non-prelim): 35
length of query: 165
length of database: 59,974,054
effective HSP length: 102
effective length of query: 63
effective length of database: 43,225,552
effective search space: 2723209776
effective search space used: 2723209776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135320.11