BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135319.8 - phase: 0 /pseudo
         (271 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

SYFB_CLOPE (Q8XJ76) Phenylalanyl-tRNA synthetase beta chain (EC ...    35  0.15
PPNK_PORGI (Q51841) Probable inorganic polyphosphate/ATP-NAD kin...    34  0.33
IF5A_NEUCR (P38672) Eukaryotic translation initiation factor 5A ...    33  0.57
IF52_YEAST (P23301) Eukaryotic translation initiation factor 5A-...    33  0.57
GALE_HAEIN (P24325) UDP-glucose 4-epimerase (EC 5.1.3.2) (Galact...    33  0.57
Y719_METJA (Q58129) Hypothetical ABC transporter ATP-binding pro...    33  0.74
MDN1_GIALA (Q8T5T1) Midasin (MIDAS-containing protein)                 33  0.74
RPOB_CYACA (Q9TM35) DNA-directed RNA polymerase beta chain (EC 2...    32  1.7
IF51_YEAST (P19211) Eukaryotic translation initiation factor 5A-...    32  1.7
ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein A)                 32  1.7
Y948_CLOAB (Q97KH1) Hypothetical UPF0230 protein CAC0948               32  2.2
GLSF_ANTSP (Q06434) Ferredoxin-dependent glutamate synthase (EC ...    32  2.2
MYSS_CHICK (P13538) Myosin heavy chain, skeletal muscle, adult         31  3.7
SMC4_HUMAN (Q9NTJ3) Structural maintenance of chromosomes 4-like...    30  4.8
ROM1_CAEEL (Q19821) Rhomboid related protein 1 (EC 3.4.21.-)           30  4.8
YG41_METJA (Q59035) Hypothetical protein MJ1641                        30  6.3
SMC4_MOUSE (Q8CG47) Structural maintenance of chromosomes 4-like...    30  6.3
LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only...    30  6.3
YGBM_HAEIN (Q57151) Hypothetical protein HI1013                        30  8.2
V117_FOWPV (Q9J5A5) Protein FPV117                                     30  8.2

>SYFB_CLOPE (Q8XJ76) Phenylalanyl-tRNA synthetase beta chain (EC
           6.1.1.20) (Phenylalanine--tRNA ligase beta chain)
           (PheRS)
          Length = 793

 Score = 35.4 bits (80), Expect = 0.15
 Identities = 28/109 (25%), Positives = 53/109 (47%), Gaps = 7/109 (6%)

Query: 168 FNTFPTVLIMDSTYKTNLYRMPLFE---IVGVTSTYLTYSVDFSFMTAEKEDNFTW---- 220
           +N  P  LI  ST K    +  L +   I  + ++ L  S+ +SF++ +  D        
Sbjct: 473 YNNIPATLISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDS 532

Query: 221 ALQMLLKLLEPNSDMPKVVVTNRDPSMMKAVTNALPDSSAILCYFHVGK 269
           +L+ ++K+  P  +   V+ T+  PSMM+++      S+ I+  F +GK
Sbjct: 533 SLRNVVKIKNPLGEDYSVMRTSTLPSMMESLARNYSRSNEIVRLFEIGK 581


>PPNK_PORGI (Q51841) Probable inorganic polyphosphate/ATP-NAD kinase
           (EC 2.7.1.23) (Poly(P)/ATP NAD kinase)
          Length = 288

 Score = 34.3 bits (77), Expect = 0.33
 Identities = 41/166 (24%), Positives = 71/166 (42%), Gaps = 25/166 (15%)

Query: 111 VYNERHKFKKTKRGDLTEMQYLISKLEENGYVHYVREKPESQTVQDIFWTHPTSVKLFNT 170
           ++  RHK ++        ++ LI KLEE G   Y+  K  S   QD+ + HP    + +T
Sbjct: 6   IFGSRHKSEQG-----ASIKALILKLEEAGTPLYIERKFLSFLKQDLDF-HPAICGVIDT 59

Query: 171 FPT----VLIM--DSTYKTNLYRMPLFEI--VGVTSTYLTYSVDF------SFMTAEKED 216
            P     V+ M  D T+    +++ + +I  +GV +  L +  D         +T   + 
Sbjct: 60  LPEHIDYVICMGGDGTFLRTAHQIGVSQIPVLGVNTGRLGFLTDVDCHEASELITRLLDG 119

Query: 217 NFTWALQMLLKLLEPNSDMP-----KVVVTNRDPSMMKAVTNALPD 257
           +FT   + LL++ E N   P     +  +  R+   M  V   L D
Sbjct: 120 DFTIETRSLLEVTEDNGSSPSYALNEAAILKRETGSMIRVNACLND 165


>IF5A_NEUCR (P38672) Eukaryotic translation initiation factor 5A
           (eIF-5A) (eIF-4D)
          Length = 163

 Score = 33.5 bits (75), Expect = 0.57
 Identities = 37/139 (26%), Positives = 53/139 (37%), Gaps = 11/139 (7%)

Query: 11  EHKLPKTKVKHEATESRKCGCLFKVRGYVVRENNAWKL----TILNGVHNHEMVRYVAGH 66
           EH          AT   +C  L K  G+VV +N   K+    T   G H H  V  VA  
Sbjct: 11  EHTFDSVDAGRSATYPMQCSALRK-GGHVVIKNRPCKIVDMSTSKTGKHGHAKVHLVAID 69

Query: 67  LLAGRLMEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQVYNERHKFKKTKRGDL 126
           +  G+ +ED     H++    VK  +   +      E    +     E  +  K   G+L
Sbjct: 70  IFTGKKLEDLCPSTHNMDVPNVKRTDYQFSY---IDEDFLVLIDSNGEEKRELKMPEGEL 126

Query: 127 TEMQYLISKLEENGYVHYV 145
            +    I KLEE G   +V
Sbjct: 127 AKR---IEKLEEEGKDFFV 142


>IF52_YEAST (P23301) Eukaryotic translation initiation factor 5A-2
          (eIF-5A 2) (eIF-4D) (Hypusine containing protein HP2)
          Length = 156

 Score = 33.5 bits (75), Expect = 0.57
 Identities = 24/83 (28%), Positives = 35/83 (41%), Gaps = 5/83 (6%)

Query: 11 EHKLPKTKVKHEATESRKCGCLFKVRGYVVRENNAWKL----TILNGVHNHEMVRYVAGH 66
          EH          AT   +C  L K  G+VV ++   K+    T   G H H  V  VA  
Sbjct: 4  EHTFETADAGSSATYPMQCSALRK-NGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAID 62

Query: 67 LLAGRLMEDDKKIVHDLTDSLVK 89
          +  G+ +ED     H++   +VK
Sbjct: 63 IFTGKKLEDLSPSTHNMEVPVVK 85


>GALE_HAEIN (P24325) UDP-glucose 4-epimerase (EC 5.1.3.2)
           (Galactowaldenase) (UDP-galactose 4-epimerase)
          Length = 338

 Score = 33.5 bits (75), Expect = 0.57
 Identities = 21/94 (22%), Positives = 43/94 (45%), Gaps = 5/94 (5%)

Query: 93  ILTNLKKKRKESITNIKQVYNERHKFKKTKRGDLTEMQYLISKLEENGYVHYVREKPESQ 152
           +L NL     +S+  +KQ+  +  KF +    D   +Q + ++ E N  +H+   K   +
Sbjct: 29  VLDNLCNSSPKSLERVKQITGKEAKFYEGDILDRALLQKIFAENEINSVIHFAGLKAVGE 88

Query: 153 TVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLY 186
           +VQ      PT   + N   T++++    K  ++
Sbjct: 89  SVQ-----KPTEYYMNNVAGTLVLIQEMKKAGVW 117


>Y719_METJA (Q58129) Hypothetical ABC transporter ATP-binding
           protein MJ0719
          Length = 600

 Score = 33.1 bits (74), Expect = 0.74
 Identities = 35/146 (23%), Positives = 65/146 (43%), Gaps = 14/146 (9%)

Query: 13  KLPKTKVKHEATESRKCGCLFKVRGYVVRENNAWKLTILNGVHNHEMVRYVAGHLLAGRL 72
           +L + K+ H   ++R     FK+ G V+  +    +   NG+    ++R +AG L+   L
Sbjct: 85  ELSEDKIVHSYGQNR-----FKLFGLVIPRDGVVGIIGQNGIGKSTVLRILAGELIP-NL 138

Query: 73  MEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQV--YNERHKFKKTKRGDLTEMQ 130
            + DK+  +D      +   +    +K + + +  I +V   +   K  K K GD     
Sbjct: 139 GKHDKEPNYDDVIKYFRGTELQEYFEKLKNKGVKAIHKVQYVDILPKVVKGKVGD----- 193

Query: 131 YLISKLEENGYVHYVREKPESQTVQD 156
            L+ K++E G    V EK E + + D
Sbjct: 194 -LLKKVDEKGKFDEVVEKLELKNILD 218


>MDN1_GIALA (Q8T5T1) Midasin (MIDAS-containing protein)
          Length = 4835

 Score = 33.1 bits (74), Expect = 0.74
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 2/52 (3%)

Query: 57   HEMVRYVAGHLLAGRLMEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNI 108
            HEMV Y+A  +L+  L + D  ++ DLT S+ K  N LT L+   +  +  I
Sbjct: 1418 HEMVLYLANRILSANLTDID--LIQDLTVSISKDTNELTFLQASTQTVLATI 1467


>RPOB_CYACA (Q9TM35) DNA-directed RNA polymerase beta chain (EC
           2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta
           subunit) (RNA polymerase beta subunit)
          Length = 1081

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 4/65 (6%)

Query: 93  ILTNLKKKRKESITNIKQVYNERHKFKKT----KRGDLTEMQYLISKLEENGYVHYVREK 148
           IL N K K  +++TN+  ++NE H  K      + G+  +   L+ K+     VH V +K
Sbjct: 898 ILINQKLKEAQTLTNLDYLFNENHLGKVALFDGRSGEKFDNPVLVGKIYMMKLVHLVDDK 957

Query: 149 PESQT 153
             S++
Sbjct: 958 IHSRS 962


>IF51_YEAST (P19211) Eukaryotic translation initiation factor 5A-1
          (eIF-5A 1) (eIF-4D) (Hypusine containing protein HP1)
          Length = 156

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 24/83 (28%), Positives = 32/83 (37%), Gaps = 5/83 (6%)

Query: 11 EHKLPKTKVKHEATESRKCGCLFKVRGYVVRENNAWKL----TILNGVHNHEMVRYVAGH 66
          EH          AT   +C  L K  G+VV +    K+    T   G H H  V  V   
Sbjct: 4  EHTFENADAGASATYPMQCSALRK-NGFVVIKGRPCKIVDMSTSKTGKHGHAKVHLVTLD 62

Query: 67 LLAGRLMEDDKKIVHDLTDSLVK 89
          +  G+ +ED     H+L    VK
Sbjct: 63 IFTGKKLEDLSPSTHNLEVPFVK 85


>ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein A)
          Length = 793

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 21/64 (32%), Positives = 31/64 (47%), Gaps = 3/64 (4%)

Query: 98  KKKRKESITNIKQVYNERHKFKKTKRGDLTEMQYLISKLEENGYVHYVREKPESQTVQDI 157
           K+  K S+ + +Q YN +    +TK     E+Q  ISKLEEN    + +   E  + QD 
Sbjct: 120 KENLKVSLESSEQKYNHKELEARTKE---EELQATISKLEENVVSLHEKLAKEESSTQDA 176

Query: 158 FWTH 161
              H
Sbjct: 177 IECH 180


>Y948_CLOAB (Q97KH1) Hypothetical UPF0230 protein CAC0948
          Length = 281

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 8/72 (11%)

Query: 82  DLTDSLVKPKNI--------LTNLKKKRKESITNIKQVYNERHKFKKTKRGDLTEMQYLI 133
           DLT+  +K  +I          + + K K  IT+ +   N   +  K+    L +M+ L 
Sbjct: 13  DLTEEFIKENDINVLSFRIIYKDKEYKDKVDITSEEVYRNFEIEVPKSSMPSLQDMEDLY 72

Query: 134 SKLEENGYVHYV 145
            KLEE GY H +
Sbjct: 73  KKLEEEGYTHAI 84


>GLSF_ANTSP (Q06434) Ferredoxin-dependent glutamate synthase (EC
            1.4.7.1) (Fd-GOGAT)
          Length = 1536

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 25/102 (24%), Positives = 49/102 (47%), Gaps = 23/102 (22%)

Query: 38   YVVRENNAWKLTILNGVHNHEMVRYVAGHLLA-----GR------------LMEDDKKIV 80
            + VR +N +  +++ GV +H    Y+ G L+      GR             +++D  + 
Sbjct: 1404 FAVRNSNGY--SVVEGVGDHAC-EYMTGGLIVVLGTFGRNIGAGMTGGIAYFLDEDNTLK 1460

Query: 81   HDLTDSLVKPKNILTNLKKKRKESITNIKQVYNERHKFKKTK 122
            + L   +VK + +LT   K+ +E + NI ++Y  + K +K K
Sbjct: 1461 NKLNTEIVKAQRLLT---KESEEQLKNIMELYEIKTKSEKAK 1499


>MYSS_CHICK (P13538) Myosin heavy chain, skeletal muscle, adult
          Length = 1938

 Score = 30.8 bits (68), Expect = 3.7
 Identities = 20/70 (28%), Positives = 30/70 (42%)

Query: 69   AGRLMEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQVYNERHKFKKTKRGDLTE 128
            A R +E D K+ HD    L   K  L    KK+   I+ I+    +        +  + E
Sbjct: 1053 AKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKE 1112

Query: 129  MQYLISKLEE 138
            +Q  I +LEE
Sbjct: 1113 LQARIEELEE 1122


>SMC4_HUMAN (Q9NTJ3) Structural maintenance of chromosomes 4-like 1
           protein (Chromosome-associated polypeptide C) (hCAP-C)
           (XCAP-C homolog)
          Length = 1288

 Score = 30.4 bits (67), Expect = 4.8
 Identities = 14/57 (24%), Positives = 31/57 (53%)

Query: 73  MEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQVYNERHKFKKTKRGDLTEM 129
           ME  K+ +H+ T  + +  NIL+N  K + + + + ++  N+  KF +  +   T++
Sbjct: 337 METQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQL 393


>ROM1_CAEEL (Q19821) Rhomboid related protein 1 (EC 3.4.21.-)
          Length = 356

 Score = 30.4 bits (67), Expect = 4.8
 Identities = 20/58 (34%), Positives = 29/58 (49%), Gaps = 8/58 (13%)

Query: 115 RHKFKKTKRGDLTE--MQYLISKLE------ENGYVHYVREKPESQTVQDIFWTHPTS 164
           RH+F + + GD TE  M  L S++E       NG +H ++E P+     D F    TS
Sbjct: 13  RHQFNQLRTGDETEIPMSTLASRIETRKIPLTNGQIHAIKEAPDELVDIDGFQKIVTS 70


>YG41_METJA (Q59035) Hypothetical protein MJ1641
          Length = 287

 Score = 30.0 bits (66), Expect = 6.3
 Identities = 16/54 (29%), Positives = 24/54 (43%)

Query: 85  DSLVKPKNILTNLKKKRKESITNIKQVYNERHKFKKTKRGDLTEMQYLISKLEE 138
           D  +  K I T L+K  K  + N+  VY       K    D+    Y ++K+EE
Sbjct: 76  DEYILMKEIKTTLEKMEKTLLNNLDLVYTALKNSDKKLAEDIIIKDYALNKMEE 129


>SMC4_MOUSE (Q8CG47) Structural maintenance of chromosomes 4-like 1
           protein (Chromosome-associated polypeptide C) (XCAP-C
           homolog)
          Length = 1286

 Score = 30.0 bits (66), Expect = 6.3
 Identities = 30/133 (22%), Positives = 63/133 (46%), Gaps = 18/133 (13%)

Query: 2   LELVCERSGEHKLPKTKV---KHEATESRKCGCLFKVRGYVVRENNAWKLTILNGVHNHE 58
           +E++ E  GE KL + K+   + +A E  K   +     ++  EN  +K        NH 
Sbjct: 272 VEILNEHRGE-KLNRVKMVEKEKDALEGEKNIAI----EFLTLENEMFKKK------NHI 320

Query: 59  MVRYVAGHLLAGRLME--DDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQVYNERH 116
              Y+  + L  R+ E    K+ +H+ T  + +  N+L+N  K +  ++ ++++  N+  
Sbjct: 321 CQYYI--YDLQNRIAEITTQKEKIHEDTKEITEKSNVLSNEMKAKNSAVKDVEKKLNKVT 378

Query: 117 KFKKTKRGDLTEM 129
           KF +  +   T++
Sbjct: 379 KFIEQNKEKFTQL 391


>LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only
           protein 20)
          Length = 1683

 Score = 30.0 bits (66), Expect = 6.3
 Identities = 22/86 (25%), Positives = 40/86 (45%), Gaps = 6/86 (6%)

Query: 54  VHNHEMVRYVAGHLLAGRLMEDDKKIVHDLTDSLVKPKNILTNLKKKRKESITNIKQVYN 113
           V N   +RY     +  +L E D+K   DL     + K+  ++L+KK++E      +   
Sbjct: 713 VQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEE------REEI 766

Query: 114 ERHKFKKTKRGDLTEMQYLISKLEEN 139
           E+   +K+KR   T  + L  +  +N
Sbjct: 767 EKQALEKSKRSSKTFKEMLQDRESQN 792


>YGBM_HAEIN (Q57151) Hypothetical protein HI1013
          Length = 258

 Score = 29.6 bits (65), Expect = 8.2
 Identities = 14/32 (43%), Positives = 18/32 (55%), Gaps = 2/32 (6%)

Query: 130 QYLISKLEENGYVHYV--REKPESQTVQDIFW 159
           QY+  KL+E GY  YV    KP  +TV  + W
Sbjct: 222 QYIFDKLDEIGYTGYVGCEYKPRGETVTGLDW 253


>V117_FOWPV (Q9J5A5) Protein FPV117
          Length = 440

 Score = 29.6 bits (65), Expect = 8.2
 Identities = 19/96 (19%), Positives = 48/96 (49%), Gaps = 13/96 (13%)

Query: 72  LMEDDKKIVHDLTDSLVK-----PKNILTNLKKKRKESITNIKQVYNERHKFKKTKRGDL 126
           ++++  K +H + D++         ++ T+L++KRK+S+ N  +   E  K  +    +L
Sbjct: 52  IIKEKFKFIHSIADNVTVFVDRGSISLKTSLREKRKQSLKNQYKRKQEELKNLEIAIDNL 111

Query: 127 T--------EMQYLISKLEENGYVHYVREKPESQTV 154
           +        + + L SK+++N Y  ++ +K   + +
Sbjct: 112 SVDDEMYEEQKESLFSKIDKNSYYMFLADKKNMEAI 147


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.319    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,919,756
Number of Sequences: 164201
Number of extensions: 1223771
Number of successful extensions: 3870
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 3859
Number of HSP's gapped (non-prelim): 29
length of query: 271
length of database: 59,974,054
effective HSP length: 108
effective length of query: 163
effective length of database: 42,240,346
effective search space: 6885176398
effective search space used: 6885176398
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 65 (29.6 bits)