BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135319.4 - phase: 0
(581 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310... 57 1e-07
TYCA_BREPA (P09095) Tyrocidine synthetase I [Includes: ATP-depen... 37 0.11
RNH1_SCHPO (Q9UST8) Ribonuclease H (EC 3.1.26.4) (RNase H) 33 1.6
YPZ5_METTF (P29579) Hypothetical 54.1 kDa protein (ORF5') 32 4.6
RW1_MOUSE (O70472) RW1 protein 32 6.0
RNH_RICPR (Q9ZCK3) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 31 7.8
RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI) (Rib... 31 7.8
DHG2_BACSU (P80869) Glucose 1-dehydrogenase II (EC 1.1.1.47) (GL... 31 7.8
COX1_SACDO (P98001) Cytochrome c oxidase polypeptide I (EC 1.9.3.1) 31 7.8
>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
(ORF154)
Length = 154
Score = 57.4 bits (137), Expect = 1e-07
Identities = 40/142 (28%), Positives = 60/142 (42%), Gaps = 3/142 (2%)
Query: 28 SIPSYFMQTNWLPQNICDSIDQTTRNFIWRGTTNKG-IHLVNWKKISLPK-NIGGLSIRA 85
++P Y M L + +C + F W NK I V W+K+ K + GGL R
Sbjct: 2 ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61
Query: 86 AREANTSLLGKLVWDMVQSSNKLWVSLLSNKYTGGHNTLYASANNRSSPTWSSIIRAKNI 145
N +LL K + ++ + L LL ++Y + + S R S W SII + +
Sbjct: 62 LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121
Query: 146 LKDGYTWRIGSG-SSSFWFSNW 166
L G IG G + W W
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRW 143
>TYCA_BREPA (P09095) Tyrocidine synthetase I [Includes: ATP-dependent
D-phenylalanine adenylase (D-PheA) (D-phenylalanine
activase); Phenylalanine racemase [ATP-hydrolyzing] (EC
5.1.1.11)]
Length = 1088
Score = 37.4 bits (85), Expect = 0.11
Identities = 36/103 (34%), Positives = 47/103 (44%), Gaps = 10/103 (9%)
Query: 43 ICDSIDQTTRNFIWRGTTNKGIHLVNWKKISLPKNIGGLSIRAAREANTSLLGKLVWDMV 102
+ D I T N R KGI K ++LP+N G LS R E + LG+ DM
Sbjct: 918 LSDYIKLTKENM--RKIPRKGIGYDILKHVTLPENRGSLSFRVQPEVTFNYLGQFDADM- 974
Query: 103 QSSNKLWVSLLSNKYTGGHNTLYASANNRSSPTWSSIIRAKNI 145
+L+ + Y+GG NTL A N SP S + A NI
Sbjct: 975 --RTELFT---RSPYSGG-NTLGADGKNNLSPE-SEVYTALNI 1010
>RNH1_SCHPO (Q9UST8) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 264
Score = 33.5 bits (75), Expect = 1.6
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 4/40 (10%)
Query: 515 LIQNIKDLLNDRNISLH----HTLREGNQCADFFAKFGAN 550
LI DL++DRN+SL H+ GNQ AD A+ GA+
Sbjct: 224 LINRASDLMSDRNVSLEYVKGHSTDYGNQQADMLARRGAS 263
>YPZ5_METTF (P29579) Hypothetical 54.1 kDa protein (ORF5')
Length = 499
Score = 32.0 bits (71), Expect = 4.6
Identities = 18/64 (28%), Positives = 30/64 (46%)
Query: 368 WRHRNLMCLNNETLSLFRLCNNIISAADAANSAFNGEENPPHSDRLVKWNNRNHQDFILN 427
W ++ +L+L L N + AN +G+ENP +++ +K+ R D LN
Sbjct: 88 WEIGDIPAYEERSLTLVCLLNRTGNVTVTANVTADGDENPANNNAQLKFRVRGIADLELN 147
Query: 428 VDGS 431
V S
Sbjct: 148 VTSS 151
>RW1_MOUSE (O70472) RW1 protein
Length = 1829
Score = 31.6 bits (70), Expect = 6.0
Identities = 27/86 (31%), Positives = 37/86 (42%), Gaps = 11/86 (12%)
Query: 92 SLLGKLVWDMVQSSNKLWVSLLSNKYTGGHNTLYASANNRSSPTW-----SSIIRAKNIL 146
++ KL QSS + W N++ G + L+ S SP+W S A +IL
Sbjct: 1684 AVFSKLSRSCSQSSQRSW-----NEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSIL 1738
Query: 147 KDGY-TWRIGSGSSSFWFSNWNSLSP 171
+ W SSS W SN NS P
Sbjct: 1739 GNSSGLWSTTPFSSSIWSSNINSNLP 1764
>RNH_RICPR (Q9ZCK3) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 152
Score = 31.2 bits (69), Expect = 7.8
Identities = 22/72 (30%), Positives = 37/72 (50%), Gaps = 7/72 (9%)
Query: 425 ILNVDGSCIGTPPRTGYGGVLR-NSAGLFISGFSGLIPNSADILLAELTAIHQGLKLVTD 483
++ DG+C G P G+G +L N I G+ + N+ E+TA + L+++
Sbjct: 6 VIYTDGACSGNPGPGGWGALLHFNDTSKKIFGYELVTTNNR----MEMTAALEALRILKK 61
Query: 484 LNVNEIMCYSDS 495
+V EI Y+DS
Sbjct: 62 SSVVEI--YTDS 71
>RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
(Ribonuclease H) (RNase H)
Length = 155
Score = 31.2 bits (69), Expect = 7.8
Identities = 15/43 (34%), Positives = 23/43 (52%), Gaps = 2/43 (4%)
Query: 429 DGSCIGTPPRTGYGGVL--RNSAGLFISGFSGLIPNSADILLA 469
DGSC+G P GYG +L R F +G++ N +++ A
Sbjct: 10 DGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAA 52
>DHG2_BACSU (P80869) Glucose 1-dehydrogenase II (EC 1.1.1.47)
(GLCDH-II) (GDH-II) (General stress protein 74) (GSP74)
Length = 258
Score = 31.2 bits (69), Expect = 7.8
Identities = 32/113 (28%), Positives = 52/113 (45%), Gaps = 17/113 (15%)
Query: 398 NSAFNGEENPPHSDRLVKWNNRNHQDFILNVDGSCIGTPPRTGYGGVLRNSAG---LFIS 454
NS FNG E PH L W + +NV G+ +G + +++N+ L IS
Sbjct: 92 NSGFNGVEAMPHEMSLEDW----QRVIDVNVTGTFLGA--KAALNHMMKNNIKGNVLNIS 145
Query: 455 GFSGLIPNSADILLAELTAIHQGLKLVTD-LNVNEIMCYSDSLLAVNLIANDT 506
IP ++ + + G+K++T+ L +N Y+D + VN IA T
Sbjct: 146 SVHQQIPRPVNV---QYSTSKGGIKMMTETLALN----YADKGIRVNAIAPGT 191
>COX1_SACDO (P98001) Cytochrome c oxidase polypeptide I (EC 1.9.3.1)
Length = 534
Score = 31.2 bits (69), Expect = 7.8
Identities = 20/69 (28%), Positives = 38/69 (54%), Gaps = 5/69 (7%)
Query: 163 FSNWNSLSPIGNYVPVID----VHDLQLSVRDVLSAPGNHARVLYTNLPPDVE-NHINNI 217
F+ WN ++ IG+++ + ++ L + + L+ N+ V+YT P VE N I N+
Sbjct: 447 FAGWNYVASIGSFIATLSLFLFIYILYDQLVNGLNNKVNNKSVIYTKAPDFVESNTIFNL 506
Query: 218 HTQFNDNIE 226
+T + +IE
Sbjct: 507 NTVKSSSIE 515
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.135 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,045,978
Number of Sequences: 164201
Number of extensions: 3205588
Number of successful extensions: 6924
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 6919
Number of HSP's gapped (non-prelim): 10
length of query: 581
length of database: 59,974,054
effective HSP length: 116
effective length of query: 465
effective length of database: 40,926,738
effective search space: 19030933170
effective search space used: 19030933170
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 69 (31.2 bits)
Medicago: description of AC135319.4