BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135311.2 + phase: 0
(164 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
IBB2_PEA (Q41066) Seed trypsin/chymotrypsin inhibitor TI5-72 pre... 130 2e-30
IBBA_PEA (Q41065) Seed trypsin/chymotrypsin inhibitor IVA precur... 107 1e-23
IBB_MEDSC (P80321) Bowman-Birk type proteinase inhibitor (MSTI) 79 4e-15
IWIT_MEDSA (P16346) Bowman-Birk type wound induced trypsin inhib... 78 8e-15
IBB_VICAN (P01065) Bowman-Birk type proteinase inhibitor (VAI) 78 1e-14
IBBB_PEA (P56679) Seed trypsin/chymotrypsin inhibitor IVB (PSTI-... 78 1e-14
IBB_VICFA (P24661) Bowman-Birk type proteinase inhibitor (FBI) 76 4e-14
IBB2_AMBCE (P83283) Bowman-birk type proteinase inhibitor 2 (TcTI2) 73 3e-13
IBB1_SOYBN (P01055) Bowman-Birk type proteinase inhibitor precur... 73 3e-13
IBB1_AMBAC (P83284) Bowman-birk type proteinase inhibitor (TaTI) 73 3e-13
IBB4_LONCA (P16343) Bowman-Birk type proteinase inhibitor DE-4 (... 72 7e-13
IBB1_PHAAN (P01058) Bowman-Birk type proteinase inhibitor 69 6e-12
IBB_VIGUN (P17734) Bowman-Birk type seed trypsin and chymotrypsi... 68 8e-12
IBB2_SOYBN (P01063) Bowman-Birk type proteinase inhibitor C-II p... 67 1e-11
IBB3_SOYBN (P01064) Bowman-Birk type proteinase inhibitor D-II p... 67 2e-11
IBB_DOLBI (Q9S9E3) Bowman-Birk type proteinase inhibitor HGI-I (... 66 4e-11
IBB3_DOLAX (P01057) Bowman-Birk type proteinase inhibitor DE-3 66 4e-11
IBB2_PHAAN (P01061) Bowman-Birk type proteinase inhibitors I-A, ... 66 4e-11
IBB1_DIOGL (P82469) Bowman-Birk type proteinase inhibitor I (DgTI) 65 5e-11
IBB_PHALU (P01056) Bowman-Birk type proteinase inhibitor 65 7e-11
>IBB2_PEA (Q41066) Seed trypsin/chymotrypsin inhibitor TI5-72
precursor
Length = 114
Score = 130 bits (326), Expect = 2e-30
Identities = 69/117 (58%), Positives = 82/117 (69%), Gaps = 14/117 (11%)
Query: 1 MELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTAT 60
MELMNK ++MKLAL+VFLL F + VV+ARFDSTSFITQV+SNGD D KS
Sbjct: 1 MELMNKK---VMMKLALMVFLLSFAANVVNARFDSTSFITQVLSNGD------DVKS--- 48
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWS-SPLCTCYDITDFCYKPCN 116
ACC+TCLC+ + T CRC DVG+TCHSAC +C C S P C C+D FCYK C+
Sbjct: 49 ACCDTCLCTKSDPPT-CRCVDVGETCHSACDSCICALSYPPQCQCFDTHKFCYKACH 104
>IBBA_PEA (Q41065) Seed trypsin/chymotrypsin inhibitor IVA precursor
(PSTI IVA) (TI12-36) [Contains: Seed
trypsin/chymotrypsin inhibitor I (PSTI I)] (Fragment)
Length = 96
Score = 107 bits (267), Expect = 1e-23
Identities = 54/96 (56%), Positives = 66/96 (68%), Gaps = 11/96 (11%)
Query: 22 LGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATACCNTCLCSVTPELTQCRCAD 81
L F + VV+ARFDSTSFITQV+SNGD D KS ACC+TCLC+ + T CRC D
Sbjct: 1 LSFAANVVNARFDSTSFITQVLSNGD------DVKS---ACCDTCLCTKSNPPT-CRCVD 50
Query: 82 VGKTCHSACKNCECGWSS-PLCTCYDITDFCYKPCN 116
V +TCHSAC +C C +S+ P C C+D FCYK C+
Sbjct: 51 VRETCHSACDSCICAYSNPPKCQCFDTHKFCYKACH 86
>IBB_MEDSC (P80321) Bowman-Birk type proteinase inhibitor (MSTI)
Length = 62
Score = 79.3 bits (194), Expect = 4e-15
Identities = 37/64 (57%), Positives = 43/64 (66%), Gaps = 6/64 (9%)
Query: 56 KSTATACCNTCLC--SVTPELTQCRCADVGKTCHSACKNCECGWS-SPLCTCYDITDFCY 112
KST TACC+ C C S+ P QC+C DV + CHSACK+C C S P C CYDITDFCY
Sbjct: 2 KSTTTACCDFCPCTRSIPP---QCQCTDVREKCHSACKSCLCTRSFPPQCRCYDITDFCY 58
Query: 113 KPCN 116
C+
Sbjct: 59 PSCS 62
>IWIT_MEDSA (P16346) Bowman-Birk type wound induced trypsin
inhibitor
Length = 58
Score = 78.2 bits (191), Expect = 8e-15
Identities = 36/60 (60%), Positives = 40/60 (66%), Gaps = 6/60 (10%)
Query: 60 TACCNTCLC--SVTPELTQCRCADVGKTCHSACKNCECGWS-SPLCTCYDITDFCYKPCN 116
TACCN C C S+ P QCRC D+G+TCHSACK C C S P C C DIT+FCY CN
Sbjct: 2 TACCNFCPCTRSIPP---QCRCTDIGETCHSACKTCLCTKSIPPQCHCADITNFCYPKCN 58
Score = 35.0 bits (79), Expect = 0.076
Identities = 16/39 (41%), Positives = 20/39 (51%), Gaps = 5/39 (12%)
Query: 54 DAKSTATACCNTCLC--SVTPELTQCRCADVGKTCHSAC 90
D T + C TCLC S+ P QC CAD+ C+ C
Sbjct: 22 DIGETCHSACKTCLCTKSIPP---QCHCADITNFCYPKC 57
>IBB_VICAN (P01065) Bowman-Birk type proteinase inhibitor (VAI)
Length = 72
Score = 77.8 bits (190), Expect = 1e-14
Identities = 31/67 (46%), Positives = 47/67 (69%), Gaps = 2/67 (2%)
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSS-PLCTCYDITDFCYKPCNVW 118
+ACC+TCLC+ + T CRC DVG+ CHSAC +C C +S+ P C C+D FCYK C+
Sbjct: 6 SACCDTCLCTRSQPPT-CRCVDVGERCHSACNHCVCNYSNPPQCQCFDTHKFCYKACHSS 64
Query: 119 QQQQKVQ 125
++++ ++
Sbjct: 65 EKEEVIK 71
>IBBB_PEA (P56679) Seed trypsin/chymotrypsin inhibitor IVB
(PSTI-IVB) [Contains: Seed trypsin/chymotrypsin
inhibitor II (PSTI II)]
Length = 72
Score = 77.8 bits (190), Expect = 1e-14
Identities = 32/58 (55%), Positives = 42/58 (72%), Gaps = 2/58 (3%)
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSS-PLCTCYDITDFCYKPCN 116
+ACC+TCLC+ + T CRC DVG+TCHSAC +C C +S+ P C C+D FCYK C+
Sbjct: 6 SACCDTCLCTKSNPPT-CRCVDVGETCHSACLSCICAYSNPPKCQCFDTQKFCYKACH 62
>IBB_VICFA (P24661) Bowman-Birk type proteinase inhibitor (FBI)
Length = 63
Score = 75.9 bits (185), Expect = 4e-14
Identities = 31/58 (53%), Positives = 41/58 (70%), Gaps = 2/58 (3%)
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSS-PLCTCYDITDFCYKPCN 116
+ACC+TCLC+ + T CRC DVG+ CHSAC +C C +S+ P C C+D FCYK C+
Sbjct: 6 SACCDTCLCTKSEPPT-CRCVDVGERCHSACNSCVCRYSNPPKCQCFDTHKFCYKSCH 62
Score = 32.0 bits (71), Expect = 0.64
Identities = 12/39 (30%), Positives = 20/39 (50%), Gaps = 1/39 (2%)
Query: 54 DAKSTATACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
D + CN+C+C + +C+C D K C+ +C N
Sbjct: 26 DVGERCHSACNSCVCRYSNP-PKCQCFDTHKFCYKSCHN 63
>IBB2_AMBCE (P83283) Bowman-birk type proteinase inhibitor 2 (TcTI2)
Length = 63
Score = 73.2 bits (178), Expect = 3e-13
Identities = 33/60 (55%), Positives = 41/60 (68%), Gaps = 7/60 (11%)
Query: 61 ACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITDFCYKPCN 116
ACC+ C C S+ P QC CAD+ +CHSAC++C C S P C C+DITDFCYKPC+
Sbjct: 6 ACCDRCACTKSIPP---QCHCADIRLNSCHSACESCACTHSIPAQCRCFDITDFCYKPCS 62
>IBB1_SOYBN (P01055) Bowman-Birk type proteinase inhibitor precursor
(BBI)
Length = 110
Score = 72.8 bits (177), Expect = 3e-13
Identities = 44/108 (40%), Positives = 61/108 (55%), Gaps = 11/108 (10%)
Query: 11 MVMKLAL-LVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATACCNTCLCS 69
+V+K+ L L+FL+G T T + R + + D +N D S CC+ C C+
Sbjct: 2 VVLKVCLVLLFLVGGT-TSANLRLSKLGLLMK----SDHQHSNDDESSKP--CCDQCACT 54
Query: 70 VTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITDFCYKPC 115
+ QCRC+D+ +CHSACK+C C S P C C DITDFCY+PC
Sbjct: 55 KSNP-PQCRCSDMRLNSCHSACKSCICALSYPAQCFCVDITDFCYEPC 101
Score = 30.8 bits (68), Expect = 1.4
Identities = 11/34 (32%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCE 94
+ C +C+C+++ QC C D+ C+ CK E
Sbjct: 73 SACKSCICALSYP-AQCFCVDITDFCYEPCKPSE 105
>IBB1_AMBAC (P83284) Bowman-birk type proteinase inhibitor (TaTI)
Length = 63
Score = 72.8 bits (177), Expect = 3e-13
Identities = 33/60 (55%), Positives = 41/60 (68%), Gaps = 7/60 (11%)
Query: 61 ACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITDFCYKPCN 116
ACC+ C C S+ P QC CAD+ +CHSAC++C C S P C C+DITDFCYKPC+
Sbjct: 6 ACCDRCACTKSIPP---QCHCADIRLNSCHSACESCACTRSIPAKCRCFDITDFCYKPCS 62
>IBB4_LONCA (P16343) Bowman-Birk type proteinase inhibitor DE-4
(DE4)
Length = 80
Score = 71.6 bits (174), Expect = 7e-13
Identities = 30/56 (53%), Positives = 39/56 (69%), Gaps = 4/56 (7%)
Query: 62 CCNTCLCSVTPELTQCRCADVG-KTCHSACKNCECGWSSP-LCTCYDITDFCYKPC 115
CC++C P QC+C DV +CHSACK+C C +S P +C+C D+TDFCYKPC
Sbjct: 18 CCSSCCTRSRPP--QCQCTDVRLNSCHSACKSCMCTFSDPGMCSCLDVTDFCYKPC 71
Score = 28.9 bits (63), Expect = 5.4
Identities = 10/32 (31%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
+ C +C+C+ + + C C DV C+ CK+
Sbjct: 43 SACKSCMCTFS-DPGMCSCLDVTDFCYKPCKS 73
>IBB1_PHAAN (P01058) Bowman-Birk type proteinase inhibitor
Length = 82
Score = 68.6 bits (166), Expect = 6e-12
Identities = 31/71 (43%), Positives = 45/71 (62%), Gaps = 5/71 (7%)
Query: 47 DSTTNNYDAKSTATACCNTCLCSVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTC 104
D TT+ + ++ CC+ C C+ + +CRC+D+ +CHSACK+C C +S P C C
Sbjct: 5 DETTD--EPSESSKPCCDQCSCTKSMP-PKCRCSDIRLNSCHSACKSCACTYSIPAKCFC 61
Query: 105 YDITDFCYKPC 115
DI DFCY+PC
Sbjct: 62 TDINDFCYEPC 72
>IBB_VIGUN (P17734) Bowman-Birk type seed trypsin and chymotrypsin
inhibitor (BTCI)
Length = 83
Score = 68.2 bits (165), Expect = 8e-12
Identities = 31/75 (41%), Positives = 45/75 (59%), Gaps = 7/75 (9%)
Query: 45 NGDSTTNNYDAKSTATACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL 101
+G + +A ++ CC C C S+ PZ CRC++V +CHSACK+C C +S P
Sbjct: 1 SGHHZBSTBZASZSSKPCCRZCACTKSIPPZ---CRCSZVRLNSCHSACKSCACTFSIPA 57
Query: 102 -CTCYDITDFCYKPC 115
C C BI BFCYKPC
Sbjct: 58 ZCFCGBIBBFCYKPC 72
>IBB2_SOYBN (P01063) Bowman-Birk type proteinase inhibitor C-II
precursor
Length = 83
Score = 67.4 bits (163), Expect = 1e-11
Identities = 29/56 (51%), Positives = 35/56 (61%), Gaps = 3/56 (5%)
Query: 62 CCNTCLCSVTPELTQCRCADVG-KTCHSACKNCECGWSSP-LCTCYDITDFCYKPC 115
CC+ C+C+ + QC CAD+ +CHSAC C C S P C C D TDFCYKPC
Sbjct: 21 CCDLCMCTASMP-PQCHCADIRLNSCHSACDRCACTRSMPGQCRCLDTTDFCYKPC 75
Score = 28.9 bits (63), Expect = 5.4
Identities = 11/34 (32%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCE 94
+ C+ C C+ + QCRC D C+ CK+ +
Sbjct: 47 SACDRCACTRSMP-GQCRCLDTTDFCYKPCKSSD 79
>IBB3_SOYBN (P01064) Bowman-Birk type proteinase inhibitor D-II
precursor (IV)
Length = 83
Score = 66.6 bits (161), Expect = 2e-11
Identities = 31/70 (44%), Positives = 41/70 (58%), Gaps = 7/70 (10%)
Query: 50 TNNYDAKSTATACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSP-LCTCY 105
+++YD + CC+ C+C S+ P QC C D+ +CHS CK+C C S P C C
Sbjct: 12 SSSYDDDEYSKPCCDLCMCTRSMPP---QCSCEDIRLNSCHSDCKSCMCTRSQPGQCRCL 68
Query: 106 DITDFCYKPC 115
D DFCYKPC
Sbjct: 69 DTNDFCYKPC 78
Score = 31.2 bits (69), Expect = 1.1
Identities = 11/30 (36%), Positives = 18/30 (59%), Gaps = 1/30 (3%)
Query: 63 CNTCLCSVTPELTQCRCADVGKTCHSACKN 92
C +C+C+ + + QCRC D C+ CK+
Sbjct: 52 CKSCMCTRS-QPGQCRCLDTNDFCYKPCKS 80
>IBB_DOLBI (Q9S9E3) Bowman-Birk type proteinase inhibitor HGI-I
(Horsegram inhibitor I) [Contains: Horsegram germinated
inhibitor I (HGGI-I); Horsegram germinated inhibitor II
(HGGI-II); Horsegram germinated inhibitor III
(HGGI-III)]
Length = 76
Score = 65.9 bits (159), Expect = 4e-11
Identities = 30/66 (45%), Positives = 39/66 (58%), Gaps = 7/66 (10%)
Query: 54 DAKSTATACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITD 109
+ ++ CC+ C C S+ P QCRC DV +CHSAC +C C +S P C C D+ D
Sbjct: 8 EPSESSKPCCDQCTCTKSIPP---QCRCTDVRLNSCHSACSSCVCTFSIPAQCVCVDMKD 64
Query: 110 FCYKPC 115
FCY PC
Sbjct: 65 FCYAPC 70
Score = 29.3 bits (64), Expect = 4.2
Identities = 10/32 (31%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
+ C++C+C+ + QC C D+ C++ CK+
Sbjct: 42 SACSSCVCTFSIP-AQCVCVDMKDFCYAPCKS 72
>IBB3_DOLAX (P01057) Bowman-Birk type proteinase inhibitor DE-3
Length = 76
Score = 65.9 bits (159), Expect = 4e-11
Identities = 30/66 (45%), Positives = 39/66 (58%), Gaps = 7/66 (10%)
Query: 54 DAKSTATACCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITD 109
+ ++ CC+ C C S+ P QCRC DV +CHSAC +C C +S P C C D+ D
Sbjct: 8 EPSESSKPCCDECACTKSIPP---QCRCTDVRLNSCHSACSSCVCTFSIPAQCVCVDMKD 64
Query: 110 FCYKPC 115
FCY PC
Sbjct: 65 FCYAPC 70
Score = 29.3 bits (64), Expect = 4.2
Identities = 10/32 (31%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
+ C++C+C+ + QC C D+ C++ CK+
Sbjct: 42 SACSSCVCTFSIP-AQCVCVDMKDFCYAPCKS 72
>IBB2_PHAAN (P01061) Bowman-Birk type proteinase inhibitors I-A,
I-B, and I-A'
Length = 78
Score = 65.9 bits (159), Expect = 4e-11
Identities = 31/58 (53%), Positives = 38/58 (65%), Gaps = 7/58 (12%)
Query: 62 CCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSP-LCTCYDITDFCYKPC 115
CC+ CLC S+ P QC+CAD+ +CHSACK+C C S P C C D DFC+KPC
Sbjct: 18 CCDLCLCTKSIPP---QCQCADIRLDSCHSACKSCMCTRSMPGQCRCLDTHDFCHKPC 72
Score = 32.3 bits (72), Expect = 0.49
Identities = 12/32 (37%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
+ C +C+C+ + QCRC D CH CK+
Sbjct: 44 SACKSCMCTRSMP-GQCRCLDTHDFCHKPCKS 74
>IBB1_DIOGL (P82469) Bowman-Birk type proteinase inhibitor I (DgTI)
Length = 67
Score = 65.5 bits (158), Expect = 5e-11
Identities = 29/56 (51%), Positives = 38/56 (67%), Gaps = 3/56 (5%)
Query: 62 CCNTCLCSVTPELTQCRCADVG-KTCHSACKNCECGWSSP-LCTCYDITDFCYKPC 115
CC+ C C+ + E QC+C DV +CHSAC+ C C S P LC+C DIT FC++PC
Sbjct: 5 CCDRCRCTKS-EPPQCQCQDVRLNSCHSACEACVCSHSMPGLCSCLDITHFCHEPC 59
Score = 30.0 bits (66), Expect = 2.4
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 3/33 (9%)
Query: 61 ACCNTCLCSVT-PELTQCRCADVGKTCHSACKN 92
+ C C+CS + P L C C D+ CH CK+
Sbjct: 31 SACEACVCSHSMPGL--CSCLDITHFCHEPCKS 61
>IBB_PHALU (P01056) Bowman-Birk type proteinase inhibitor
Length = 83
Score = 65.1 bits (157), Expect = 7e-11
Identities = 30/58 (51%), Positives = 37/58 (63%), Gaps = 7/58 (12%)
Query: 62 CCNTCLC--SVTPELTQCRCADVG-KTCHSACKNCECGWSSPL-CTCYDITDFCYKPC 115
CC+ C C S+ P QCRC D+ +CHSACK+C C S P C C BI DFCY+PC
Sbjct: 18 CCBHCACTKSIPP---QCRCTDLRLDSCHSACKSCICTLSIPAQCVCBBIBDFCYEPC 72
Score = 29.3 bits (64), Expect = 4.2
Identities = 9/32 (28%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKN 92
+ C +C+C+++ QC C B+ C+ CK+
Sbjct: 44 SACKSCICTLSIP-AQCVCBBIBDFCYEPCKS 74
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.131 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,387,004
Number of Sequences: 164201
Number of extensions: 642685
Number of successful extensions: 3114
Number of sequences better than 10.0: 247
Number of HSP's better than 10.0 without gapping: 65
Number of HSP's successfully gapped in prelim test: 183
Number of HSP's that attempted gapping in prelim test: 2771
Number of HSP's gapped (non-prelim): 441
length of query: 164
length of database: 59,974,054
effective HSP length: 102
effective length of query: 62
effective length of database: 43,225,552
effective search space: 2679984224
effective search space used: 2679984224
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135311.2