BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135160.10 + phase: 0
(373 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
R8L2_ARATH (Q9MAG6) Probable disease resistance RPP8-like protein 2 50 1e-05
PYR1_EMENI (O93937) PyrABCN protein [Includes: Glutamine-depende... 33 0.89
FA8_HUMAN (P00451) Coagulation factor VIII precursor (Procoagula... 33 1.5
GAN_HUMAN (Q9H2C0) Gigaxonin (Kelch-like protein 16) 32 2.6
SYL_FUSNN (Q8RIQ3) Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine-... 32 3.4
AROC_BUCAI (P57198) Chorismate synthase (EC 4.2.3.5) (5-enolpyru... 32 3.4
AEC1_ARATH (Q9C6B8) Auxin efflux carrier component 1 (PIN-FORMED... 31 4.4
AROC_BUCAP (Q9ZHE9) Chorismate synthase (EC 4.2.3.5) (5-enolpyru... 31 5.8
TOP1_CAMJE (Q9PLZ2) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 30 7.6
RPOA_OENHO (Q9MTJ3) DNA-directed RNA polymerase alpha chain (EC ... 30 7.6
YF09_MYCPN (P75277) Hypothetical protein MPN509 (P02_orf427) 30 9.9
AROC_BUCBP (Q89AX9) Chorismate synthase (EC 4.2.3.5) (5-enolpyru... 30 9.9
>R8L2_ARATH (Q9MAG6) Probable disease resistance RPP8-like protein 2
Length = 1271
Score = 49.7 bits (117), Expect = 1e-05
Identities = 63/275 (22%), Positives = 107/275 (38%), Gaps = 24/275 (8%)
Query: 38 LPTKSLSRFKCVQKSWN---NIIKSPYFATRRNRLLILQNAPNMKFIFCDGGNDQKSIPI 94
+P KS++RF+CV +S + ++ + + L+ N +F Q
Sbjct: 919 VPAKSIARFRCVSESIHRRPDVTEQSLTKSMSRPRLLFALEVNKDLLFFSSPQPQNPYDN 978
Query: 95 KSLFPQDVARIEIY---GSCDGVFCLKGISSCITRHDQLILWNPTTKEVHLIPRAPSLGN 151
SL R Y C + L + + + ++I NP + + +P+ + G
Sbjct: 979 SSLVATPYKRFPKYLPTRICTTLGGLVFLQKWLRKKTRVIC-NPVSGDYITLPKVKATG- 1036
Query: 152 HYSDESLYGFGAVNDDFKVVKLNISNSNRMAKINSLLKADIYDLSTKSWTPLVSHPPITM 211
ES +GF + FKV+ + S L L P++ H
Sbjct: 1037 --VGESYFGFDPITKQFKVLCMTWSRYGTPNTHQVLTLETGKRLWRTIQDPILPH----- 1089
Query: 212 VTRIQPSRYNTLVNGVYYWITSSDGSDAARILCFDFRDNQFRKLEAPKLGHYIPFFCDDV 271
S +NGV Y+ + S +++I+CFDFR +F + G + + +
Sbjct: 1090 ----YRSFDRICINGVLYYGADFEESQSSKIVCFDFRFEKFSFINIADEGMFRGSYKWTL 1145
Query: 272 FEIKGYLGYVVQYRCRIVWLEIWTL---EQNGWAK 303
F KG LG QY R L +W L E + W+K
Sbjct: 1146 FNYKGKLG-AHQY-SRNGELVLWVLEDAENHKWSK 1178
>PYR1_EMENI (O93937) PyrABCN protein [Includes: Glutamine-dependent
carbamoyl-phosphate (EC 6.3.5.5); Aspartate
carbamoyltransferase (EC 2.1.3.2)]
Length = 2275
Score = 33.5 bits (75), Expect = 0.89
Identities = 14/53 (26%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Query: 161 FGAVNDDFKVVKLNISNSNRMAKINSLLKADIYDLSTKSW--TPLVSHPPITM 211
F A ++D KV++ N+ S ++ ++ D+ +++TK+ P +PP+T+
Sbjct: 1290 FIAKDNDIKVIECNVRASRSFPFVSKVMGVDLIEMATKAMIGAPFAEYPPVTI 1342
>FA8_HUMAN (P00451) Coagulation factor VIII precursor (Procoagulant
component) (Antihemophilic factor) (AHF)
Length = 2351
Score = 32.7 bits (73), Expect = 1.5
Identities = 33/150 (22%), Positives = 56/150 (37%), Gaps = 5/150 (3%)
Query: 15 RKTKLSIERMKLGEELEIEILLRLPTKSLSRFKCVQKSWNNIIKSPYFATRRNRLLI--- 71
R K + K GEE +E L + + ++ C + N + + R R L
Sbjct: 1279 RTKKHTAHFSKKGEEENLEGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFR 1338
Query: 72 --LQNAPNMKFIFCDGGNDQKSIPIKSLFPQDVARIEIYGSCDGVFCLKGISSCITRHDQ 129
L+ K I D + Q S +K L P + +I+ G +S C+TR
Sbjct: 1339 LPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHS 1398
Query: 130 LILWNPTTKEVHLIPRAPSLGNHYSDESLY 159
+ N + + + PS+ Y L+
Sbjct: 1399 IPQANRSPLPIAKVSSFPSIRPIYLTRVLF 1428
>GAN_HUMAN (Q9H2C0) Gigaxonin (Kelch-like protein 16)
Length = 597
Score = 32.0 bits (71), Expect = 2.6
Identities = 25/98 (25%), Positives = 43/98 (43%), Gaps = 10/98 (10%)
Query: 155 DESLYGFGAVNDDFKVVKLNISNSNRMAKINSLLKADIYDLSTKSWTPLVSHPPITMVTR 214
+E+ + FG V D + L + + L+ + YD+ +K+WT P +TMV +
Sbjct: 361 NEARHNFGIVEIDGMLYILGGEDGEK-----ELISMECYDIYSKTWT---KQPDLTMVRK 412
Query: 215 IQPSRYNTLVNGVYYWITSSDGSDAARILCFDFRDNQF 252
I Y + +Y S G + C+D R Q+
Sbjct: 413 I--GCYAAMKKKIYAMGGGSYGKLFESVECYDPRTQQW 448
>SYL_FUSNN (Q8RIQ3) Leucyl-tRNA synthetase (EC 6.1.1.4)
(Leucine--tRNA ligase) (LeuRS)
Length = 859
Score = 31.6 bits (70), Expect = 3.4
Identities = 34/124 (27%), Positives = 56/124 (44%), Gaps = 19/124 (15%)
Query: 169 KVVKLNISNSNRMAKINSLLKADIYDLSTKSWTPLVS-HPPITMVTRIQPSRYN------ 221
+V +L NS + N + D LS + T L+ + I VT + Y+
Sbjct: 664 RVWRLVFENSELVKNAND--EIDYNKLSKEDKTLLIKLNQTIKKVTDAIENNYHFNTAIA 721
Query: 222 ---TLVNGVYYWITSSDGSD-AARILCFDFRDNQFRKLEAPKLGHYIPFFCDDVFEIKGY 277
L+N V +++SS S+ AA+IL + K L ++P FCD+++E G
Sbjct: 722 ANMELINEVQTYVSSSMNSEQAAKILGYTL------KKIIIMLSPFVPHFCDEIWEELGE 775
Query: 278 LGYV 281
GY+
Sbjct: 776 KGYL 779
>AROC_BUCAI (P57198) Chorismate synthase (EC 4.2.3.5)
(5-enolpyruvylshikimate-3-phosphate phospholyase)
Length = 354
Score = 31.6 bits (70), Expect = 3.4
Identities = 12/41 (29%), Positives = 22/41 (53%)
Query: 34 ILLRLPTKSLSRFKCVQKSWNNIIKSPYFATRRNRLLILQN 74
I +R ++ KC KSW + +P+F + ++L L+N
Sbjct: 151 ITIRAYLSAMGNIKCPFKSWQEVENNPFFCSDPEKILALEN 191
>AEC1_ARATH (Q9C6B8) Auxin efflux carrier component 1 (PIN-FORMED
protein) (AtPIN1)
Length = 622
Score = 31.2 bits (69), Expect = 4.4
Identities = 22/79 (27%), Positives = 34/79 (42%), Gaps = 5/79 (6%)
Query: 134 NPTTKEVHL-IPRAPSLGNHYSDESLYGFGAVNDDFKVVKL----NISNSNRMAKINSLL 188
N K+V + +P+ S N Y + + FG +DD KV+ NISN AK+
Sbjct: 399 NDHQKDVKISVPQGNSNDNQYVEREEFSFGNKDDDSKVLATDGGNNISNKTTQAKVMPPT 458
Query: 189 KADIYDLSTKSWTPLVSHP 207
+ W L+ +P
Sbjct: 459 SVMTRLILIMVWRKLIRNP 477
>AROC_BUCAP (Q9ZHE9) Chorismate synthase (EC 4.2.3.5)
(5-enolpyruvylshikimate-3-phosphate phospholyase)
Length = 353
Score = 30.8 bits (68), Expect = 5.8
Identities = 10/45 (22%), Positives = 27/45 (59%)
Query: 29 ELEIEILLRLPTKSLSRFKCVQKSWNNIIKSPYFATRRNRLLILQ 73
+++ I++R ++ KC +SW + ++P+F + +N++ L+
Sbjct: 146 KIQTGIVIRAYLSAMGDIKCPFESWEEVEQNPFFCSNKNKVFQLE 190
>TOP1_CAMJE (Q9PLZ2) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 700
Score = 30.4 bits (67), Expect = 7.6
Identities = 19/59 (32%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Query: 166 DDFKVVKLNISNSNRMAKINSLLKADIYDLS-TKSWTPLVSHPPITMVTRIQPSRYNTL 223
D K+ KL I+N +R I K ++Y +S +S ++ PP M + +Q S N L
Sbjct: 210 DKAKIEKLTITNKDRAKLILEACKNEVYSISDIESKERKIAPPPPFMTSTLQQSASNRL 268
>RPOA_OENHO (Q9MTJ3) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase alpha
subunit) (RNA polymerase alpha subunit)
Length = 367
Score = 30.4 bits (67), Expect = 7.6
Identities = 16/44 (36%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Query: 89 QKSIP-IKSLFPQDVARIEIYGSCDGVFCLKGISSCITRHDQLI 131
Q+S+P I + V R +YG+C+ C+KG C+T D L+
Sbjct: 82 QESVPEILMNLKEIVLRSNLYGTCNASICVKG-PGCVTAQDILL 124
>YF09_MYCPN (P75277) Hypothetical protein MPN509 (P02_orf427)
Length = 427
Score = 30.0 bits (66), Expect = 9.9
Identities = 24/81 (29%), Positives = 39/81 (47%), Gaps = 15/81 (18%)
Query: 6 VHNKKFSRLRKTKLSIERMKLGEELEIEILLRLPTKSLSRFKCVQ--------KSWNNII 57
+H+++ +RL + + +E G+E +++ L+R T+ LS F V+ K N I
Sbjct: 119 IHDEEQARLERERKQLEEELKGKEKKVQELIREKTRFLSSFNNVKSFKDYWKGKGKNVEI 178
Query: 58 KSPY-------FATRRNRLLI 71
KS F T RNR I
Sbjct: 179 KSQLIEVLKLAFKTDRNRTFI 199
>AROC_BUCBP (Q89AX9) Chorismate synthase (EC 4.2.3.5)
(5-enolpyruvylshikimate-3-phosphate phospholyase)
Length = 355
Score = 30.0 bits (66), Expect = 9.9
Identities = 12/29 (41%), Positives = 17/29 (58%)
Query: 34 ILLRLPTKSLSRFKCVQKSWNNIIKSPYF 62
IL+R K + C KSWN + K+P+F
Sbjct: 151 ILIRGYLKQIGNITCKLKSWNEVEKNPFF 179
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.140 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,382,592
Number of Sequences: 164201
Number of extensions: 1908655
Number of successful extensions: 3529
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3524
Number of HSP's gapped (non-prelim): 12
length of query: 373
length of database: 59,974,054
effective HSP length: 112
effective length of query: 261
effective length of database: 41,583,542
effective search space: 10853304462
effective search space used: 10853304462
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 66 (30.0 bits)
Medicago: description of AC135160.10