BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC134521.1 - phase: 0 /pseudo
         (324 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TRUD_METAC (Q8TKX2) Probable tRNA pseudouridine synthase D (EC 4...    30  8.2
GUAA_HELHP (Q7VG78) Probable GMP synthase [glutamine-hydrolyzing...    30  8.2

>TRUD_METAC (Q8TKX2) Probable tRNA pseudouridine synthase D (EC
           4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase)
          Length = 441

 Score = 30.0 bits (66), Expect = 8.2
 Identities = 36/184 (19%), Positives = 70/184 (37%), Gaps = 34/184 (18%)

Query: 77  QLFPTLEEYSHWVGLPVLD-----EVPFHAFGPIPKIPTIAKALHLEVADIKDKFTTKDG 131
           Q F ++   +H VG  +++         +   P P+ P   KA    V + +D    K+G
Sbjct: 176 QRFGSVRSVTHLVGKAIVEGNFEKAAMLYIAEPFPEEPEETKAARQFVKETRD---FKEG 232

Query: 132 LPCLPYNFLHQKATTCFEKSKTDDFESILALL---------------IYGIVLFPKVDKF 176
           L   P    H++A      S  +D+    ++L               IY ++L  +++  
Sbjct: 233 LKTYPLRLGHERAMMNHLISNPEDYSGAFSVLPKNLYRMFVHAYQSYIYNMILCRRIESG 292

Query: 177 VDMNA-----IRIFLTQNPVP------VLLADTYVAIHDRTSHGKGTIVCCTPLLHRWIT 225
           + +N      I  F  +  +P       + ++T  A++    HG+  I    P  +    
Sbjct: 293 ISLNRAVEGDIVCFRNEAGLPDSSKTEKVTSETVNAMNRLIKHGRAFITAPLPGFNTEFA 352

Query: 226 SHLP 229
           S +P
Sbjct: 353 SGVP 356


>GUAA_HELHP (Q7VG78) Probable GMP synthase [glutamine-hydrolyzing]
           (EC 6.3.5.2) (Glutamine amidotransferase) (GMP
           synthetase)
          Length = 1375

 Score = 30.0 bits (66), Expect = 8.2
 Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 2/39 (5%)

Query: 272 NNVPLLGTQGGISYSPILARRQFEY--YTEMKPVYLILD 308
           NNV +L    G  Y+ ++ARR  EY  YTE+ P +  LD
Sbjct: 4   NNVQILVLDFGSQYTQLIARRLREYGVYTEIVPYFEKLD 42


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.324    0.144    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,478,654
Number of Sequences: 164201
Number of extensions: 1985698
Number of successful extensions: 3375
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3375
Number of HSP's gapped (non-prelim): 2
length of query: 324
length of database: 59,974,054
effective HSP length: 110
effective length of query: 214
effective length of database: 41,911,944
effective search space: 8969156016
effective search space used: 8969156016
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 66 (30.0 bits)