BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134049.16 - phase: 0
(166 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VIRN_NICGU (Q40392) TMV resistance protein N 148 6e-36
WR16_ARATH (Q9FL92) Probable WRKY transcription factor 16 (WRKY ... 56 3e-08
WR52_ARATH (Q9FH83) Probable WRKY transcription factor 52 (WRKY ... 42 6e-04
WR19_ARATH (Q9SZ67) Probable WRKY transcription factor 19 (WRKY ... 40 0.002
TOLL_DROME (P08953) Toll protein precursor 34 0.17
TLR2_MOUSE (Q9QUN7) Toll-like receptor 2 precursor 33 0.39
TLR2_CRIGR (Q9R1F8) Toll-like receptor 2 precursor 32 0.66
TLR2_HUMAN (O60603) Toll-like receptor 2 precursor (Toll/interle... 31 1.1
TLR2_MACFA (Q95M53) Toll-like receptor 2 precursor 30 1.9
RBP2_PLAVB (Q00799) Reticulocyte binding protein 2 precursor (Pv... 30 1.9
TLR2_BOVIN (Q95LA9) Toll-like receptor 2 precursor 30 2.5
T2B1_HERAU (P25257) Type II restriction enzyme HgiBI (EC 3.1.21.... 30 2.5
ENV_OMVVS (P16899) ENV polyprotein precursor (Coat polyprotein) 30 2.5
DGAL_ECOLI (P02927) D-galactose-binding periplasmic protein prec... 30 2.5
RL9_CHLCV (Q821W9) 50S ribosomal protein L9 29 4.3
APBE_CHLPN (Q9Z8K2) Thiamine biosynthesis lipoprotein apbE precu... 29 4.3
T2E1_HERAU (P25260) Type II restriction enzyme HgiEI (EC 3.1.21.... 29 5.6
ENV_VILV2 (P23423) ENV polyprotein precursor (Coat polyprotein) 29 5.6
DPOL_THEG8 (Q9HH84) DNA polymerase (EC 2.7.7.7) [Contains: Endon... 29 5.6
DGAL_SALTY (P23905) D-galactose-binding periplasmic protein prec... 29 5.6
>VIRN_NICGU (Q40392) TMV resistance protein N
Length = 1144
Score = 148 bits (373), Expect = 6e-36
Identities = 78/156 (50%), Positives = 107/156 (68%), Gaps = 8/156 (5%)
Query: 6 SSSSSSFNNAPLEKHEVFISFRGEDTRNNFTSHLNGALKRLDIRTYIDND-LNSGDEIPT 64
SSSSS ++ ++VF+SFRGEDTR FTSHL L I+T+ D+ L G IP
Sbjct: 4 SSSSSRWS------YDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPG 57
Query: 65 TLVRAIEEAKLSVIVFSKNYAVSKWCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQR 124
L +AIEE++ +++VFS+NYA S+WCL EL+KI+E K Q V+P+FYDVDPS VRNQ+
Sbjct: 58 ELCKAIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQK 117
Query: 125 GSYAEAFAKHENNFEGKIK-VQEWRNGLLEAANYAG 159
S+A+AF +HE ++ ++ +Q WR L EAAN G
Sbjct: 118 ESFAKAFEEHETKYKDDVEGIQRWRIALNEAANLKG 153
>WR16_ARATH (Q9FL92) Probable WRKY transcription factor 16 (WRKY
DNA-binding protein 16)
Length = 1372
Score = 56.2 bits (134), Expect = 3e-08
Identities = 34/92 (36%), Positives = 55/92 (58%), Gaps = 8/92 (8%)
Query: 29 EDTRNNFTSHLNGALKRLDIR-TYIDNDLNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVS 87
E+ R +F SHL+ AL+R + +ID+D D + +E A++SV++ N VS
Sbjct: 14 EEVRYSFVSHLSKALQRKGVNDVFIDSD----DSLSNESQSMVERARVSVMILPGNRTVS 69
Query: 88 KWCLEELMKILEIKKMKGQIVVPVFYDVDPSD 119
L++L+K+L+ +K K Q+VVPV Y V S+
Sbjct: 70 ---LDKLVKVLDCQKNKDQVVVPVLYGVRSSE 98
>WR52_ARATH (Q9FH83) Probable WRKY transcription factor 52 (WRKY
DNA-binding protein 52) (Disease resistance protein
RRS1) (Resistance to Ralstonia solanacearum 1 protein)
Length = 1378
Score = 42.0 bits (97), Expect = 6e-04
Identities = 34/98 (34%), Positives = 52/98 (52%), Gaps = 3/98 (3%)
Query: 18 EKHEVFISFRG-EDTRNNFTSHLNGALKRLDIRTYIDNDLNSGDEIPTTLVRAIEEAKLS 76
EK E F+ E+ R +F SHL+ AL+R I + D++ D + IE+A +S
Sbjct: 5 EKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVV-VDVDIDDLLFKESQAKIEKAGVS 63
Query: 77 VIVFSKNYAVSKWCLEELMKILEIKK-MKGQIVVPVFY 113
V+V N S+ L++ K+LE ++ K Q VV V Y
Sbjct: 64 VMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLY 101
>WR19_ARATH (Q9SZ67) Probable WRKY transcription factor 19 (WRKY
DNA-binding protein 19)
Length = 1895
Score = 40.4 bits (93), Expect = 0.002
Identities = 35/142 (24%), Positives = 60/142 (41%), Gaps = 24/142 (16%)
Query: 20 HEVFISFRGEDTRN-NFTSHLNGALKRLDIRTYIDNDLNSGDEIPTTLVRAIEEAKLSVI 78
++V I + D N +F SHL +L R I Y N D +P + ++ +I
Sbjct: 668 YDVVIRYGRADISNEDFISHLRASLCRRGISVY--EKFNEVDALP--------KCRVLII 717
Query: 79 VFSKNYAVSKWCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAFAKHENNF 138
V + Y S L+ ILE + + ++V P+FY + P D +Y + + E
Sbjct: 718 VLTSTYVPSN-----LLNILEHQHTEDRVVYPIFYRLSPYDFVCNSKNYERFYLQDE--- 769
Query: 139 EGKIKVQEWRNGLLEAANYAGW 160
++W+ L E G+
Sbjct: 770 -----PKKWQAALKEITQMPGY 786
>TOLL_DROME (P08953) Toll protein precursor
Length = 1097
Score = 33.9 bits (76), Expect = 0.17
Identities = 21/103 (20%), Positives = 51/103 (49%), Gaps = 6/103 (5%)
Query: 18 EKHEVFISFRGEDT---RNNFTSHLNGALKRLDIRTYIDNDLNSGDEIPTTLVRAIEEAK 74
+K + FIS+ +D + L ++ + + + D G IP ++R++ +++
Sbjct: 857 KKFDAFISYSHKDQSFIEDYLVPQLEHGPQKFQLCVH-ERDWLVGGHIPENIMRSVADSR 915
Query: 75 LSVIVFSKNYAVSKWCLEELMKI--LEIKKMKGQIVVPVFYDV 115
++IV S+N+ S+W E + + + +I+V ++ D+
Sbjct: 916 RTIIVLSQNFIKSEWARLEFRAAHRSALNEGRSRIIVIIYSDI 958
>TLR2_MOUSE (Q9QUN7) Toll-like receptor 2 precursor
Length = 784
Score = 32.7 bits (73), Expect = 0.39
Identities = 23/99 (23%), Positives = 44/99 (44%), Gaps = 6/99 (6%)
Query: 1 MAWSYSSSSSSFNNAPLEK--HEVFISFRGEDTR---NNFTSHLNGALKRLDIRTYIDND 55
M W++ + AP ++ F+S+ +D+ N L + + + D
Sbjct: 620 MMWAWLQAKRKPKKAPCRDVCYDAFVSYSEQDSHWVENLMVQQLENSDPPFKLCLH-KRD 678
Query: 56 LNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEEL 94
G I ++ +IE++ +V V S+N+ S+WC EL
Sbjct: 679 FVPGKWIIDNIIDSIEKSHKTVFVLSENFVRSEWCKYEL 717
>TLR2_CRIGR (Q9R1F8) Toll-like receptor 2 precursor
Length = 784
Score = 32.0 bits (71), Expect = 0.66
Identities = 22/99 (22%), Positives = 44/99 (44%), Gaps = 6/99 (6%)
Query: 1 MAWSYSSSSSSFNNAPLEK--HEVFISFRGEDT---RNNFTSHLNGALKRLDIRTYIDND 55
M W++ + AP ++ F+S+ +D+ N L + + + D
Sbjct: 620 MMWAWLQAKRKPKKAPCRDICYDAFVSYSEQDSYWVENLMVQQLENSEPPFKLCLH-KRD 678
Query: 56 LNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEEL 94
G I ++ +IE++ ++ V S+N+ S+WC EL
Sbjct: 679 FVPGKWIIDNIIDSIEKSHKTLFVLSENFVRSEWCKYEL 717
>TLR2_HUMAN (O60603) Toll-like receptor 2 precursor
(Toll/interleukin 1 receptor-like protein 4)
Length = 784
Score = 31.2 bits (69), Expect = 1.1
Identities = 23/99 (23%), Positives = 41/99 (41%), Gaps = 6/99 (6%)
Query: 1 MAWSYSSSSSSFNNAPLEK--HEVFISFRGEDT---RNNFTSHLNGALKRLDIRTYIDND 55
M W++ + AP ++ F+S+ D N L + + D
Sbjct: 620 MMWAWLQAKRKPRKAPSRNICYDAFVSYSERDAYWVENLMVQELENFNPPFKLCLH-KRD 678
Query: 56 LNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEEL 94
G I ++ +IE++ +V V S+N+ S+WC EL
Sbjct: 679 FIPGKWIIDNIIDSIEKSHKTVFVLSENFVKSEWCKYEL 717
>TLR2_MACFA (Q95M53) Toll-like receptor 2 precursor
Length = 784
Score = 30.4 bits (67), Expect = 1.9
Identities = 14/40 (35%), Positives = 23/40 (57%)
Query: 55 DLNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEEL 94
D G I ++ +IE++ +V V S+N+ S+WC EL
Sbjct: 678 DFIPGKWIIDNIIDSIEKSHKTVFVLSENFVKSEWCKYEL 717
>RBP2_PLAVB (Q00799) Reticulocyte binding protein 2 precursor
(PvRBP-2)
Length = 2867
Score = 30.4 bits (67), Expect = 1.9
Identities = 27/129 (20%), Positives = 58/129 (44%), Gaps = 18/129 (13%)
Query: 29 EDTRNNFTSHLNGALKRLDIRTYIDNDLNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSK 88
+D ++N+ ++LN ++ N LN D T + A++E+K NY +
Sbjct: 1662 KDDQSNYVNYLNQITTERNLIVTEKNRLNGIDSTITNIEGALKESK-------GNYEIG- 1713
Query: 89 WCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAFAKHE-NNFEGKIKVQEW 147
LE+L +I + +K+K D+ + + G+++ F + N ++ + ++
Sbjct: 1714 -FLEKLEEIGKNRKLK--------VDITKKSINSTVGNFSSLFNNFDLNQYDFNKNINDY 1764
Query: 148 RNGLLEAAN 156
N + E N
Sbjct: 1765 ENKMGEIYN 1773
>TLR2_BOVIN (Q95LA9) Toll-like receptor 2 precursor
Length = 784
Score = 30.0 bits (66), Expect = 2.5
Identities = 13/40 (32%), Positives = 23/40 (57%)
Query: 55 DLNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEEL 94
D G I ++ +IE++ ++ V S+N+ S+WC EL
Sbjct: 678 DFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYEL 717
>T2B1_HERAU (P25257) Type II restriction enzyme HgiBI (EC 3.1.21.4)
(Endonuclease HgiBI) (R.HgiBI)
Length = 274
Score = 30.0 bits (66), Expect = 2.5
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 3/71 (4%)
Query: 90 CLEELMKILEIKKMKGQIVVPV--FYDVDPSDVRNQRGSYAEAFAKHENNFEGKIKV-QE 146
CLE + ++L I +K Q VPV FY + + R SY + K +F + + QE
Sbjct: 180 CLEVMQRLLRIYTIKQQSAVPVKAFYAMAYNPWGISRASYRSSITKKYTDFSNAVVIGQE 239
Query: 147 WRNGLLEAANY 157
+ + + E + Y
Sbjct: 240 FWSLIGEPSTY 250
>ENV_OMVVS (P16899) ENV polyprotein precursor (Coat polyprotein)
Length = 990
Score = 30.0 bits (66), Expect = 2.5
Identities = 17/74 (22%), Positives = 31/74 (40%)
Query: 75 LSVIVFSKNYAVSKWCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAFAKH 134
+ VI F V CL+ ++ EI+ + +V+ D++ + GS +H
Sbjct: 847 VGVICFRLLMCVITMCLQAYRQVREIRYTRVTVVIEAPVDLEEKQREERDGSSGSENLEH 906
Query: 135 ENNFEGKIKVQEWR 148
E + +Q WR
Sbjct: 907 EKRTSPRSFIQIWR 920
>DGAL_ECOLI (P02927) D-galactose-binding periplasmic protein
precursor (GBP) (D-galactose/ D-glucose binding protein)
(GGBP)
Length = 332
Score = 30.0 bits (66), Expect = 2.5
Identities = 23/69 (33%), Positives = 34/69 (48%), Gaps = 14/69 (20%)
Query: 100 IKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAF-----AKHENNFEGKIKVQEWRNGLLEA 154
I+K +GQ V VF++ +PS R SY +A+ +K +G + + W A
Sbjct: 100 IEKARGQNVPVVFFNKEPS--RKALDSYDKAYYVGTDSKESGIIQGDLIAKHW------A 151
Query: 155 ANYAGWDCN 163
AN GWD N
Sbjct: 152 AN-QGWDLN 159
>RL9_CHLCV (Q821W9) 50S ribosomal protein L9
Length = 172
Score = 29.3 bits (64), Expect = 4.3
Identities = 25/96 (26%), Positives = 45/96 (46%), Gaps = 7/96 (7%)
Query: 46 LDIRTYIDNDLNSGDEIPTTLVRAIEEA-KLSVIVFSKNYAVSKWCLEELMKILEIKKMK 104
L+ + +D D N + T+ I+EA K ++I+ KN+ S + ++ L K K+K
Sbjct: 79 LEFQVRVDPDNNMYGSV--TISDIIDEAAKKNIILTRKNFPHSHYAIKNLGKKSVPLKLK 136
Query: 105 GQIVVPVFYDVDPSD----VRNQRGSYAEAFAKHEN 136
+ +F +V V NQ+ + E A+ N
Sbjct: 137 EDVTATLFVEVSSESSYVAVLNQQPTQEEPAAEESN 172
>APBE_CHLPN (Q9Z8K2) Thiamine biosynthesis lipoprotein apbE
precursor
Length = 314
Score = 29.3 bits (64), Expect = 4.3
Identities = 15/62 (24%), Positives = 29/62 (46%)
Query: 86 VSKWCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAFAKHENNFEGKIKVQ 145
+ KWC+E + + G+ + Y + V + +YA+A A F+ KI+ +
Sbjct: 237 IQKWCVEGKIYTHILDTRTGKPLELSSYPIQSVSVVHPSCAYADAIATVLMTFDSKIEAK 296
Query: 146 EW 147
+W
Sbjct: 297 QW 298
>T2E1_HERAU (P25260) Type II restriction enzyme HgiEI (EC 3.1.21.4)
(Endonuclease HgiEI) (R.HgiEI)
Length = 274
Score = 28.9 bits (63), Expect = 5.6
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 3/71 (4%)
Query: 90 CLEELMKILEIKKMKGQIVVPV--FYDVDPSDVRNQRGSYAEAFAKHENNFEGKIKV-QE 146
CLE + ++L I +K Q VPV FY + + R SY + K +F + + QE
Sbjct: 180 CLEVMQRLLRIYTIKQQSAVPVKAFYAMAYNPWGISRASYRSSNTKKYTDFSNAVVIGQE 239
Query: 147 WRNGLLEAANY 157
+ + + E + Y
Sbjct: 240 FWSLIGEPSTY 250
>ENV_VILV2 (P23423) ENV polyprotein precursor (Coat polyprotein)
Length = 991
Score = 28.9 bits (63), Expect = 5.6
Identities = 16/74 (21%), Positives = 33/74 (43%)
Query: 75 LSVIVFSKNYAVSKWCLEELMKILEIKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAFAKH 134
+ +I F V CL+ ++ +I+ + +V+ +++ RN G+ A +H
Sbjct: 849 VGLICFRILMCVISMCLQAYKQVKQIRYTQVTVVIEAPVELEEKQKRNGDGTNGCASLEH 908
Query: 135 ENNFEGKIKVQEWR 148
E + +Q WR
Sbjct: 909 ERRTSHRSFIQIWR 922
>DPOL_THEG8 (Q9HH84) DNA polymerase (EC 2.7.7.7) [Contains:
Endonuclease PI-TspGE8I (EC 3.1.-.-) (Tsp-GE8 pol-1
intein); Endonuclease PI-TspGE8II (EC 3.1.-.-) (Tsp-GE8
pol-2 intein)]
Length = 1699
Score = 28.9 bits (63), Expect = 5.6
Identities = 23/87 (26%), Positives = 36/87 (40%), Gaps = 14/87 (16%)
Query: 39 LNGALKRLDIRTYIDNDLNSGDEIPTTLVRAIEEAKLSVIVFSKNYAVSKWCLEELMKIL 98
+NG LK + I ++DN + G + +E + + I F++ KI
Sbjct: 503 VNGRLKLVRIGDFVDNTMKKGQPLENDGTEVLEVSGIEAISFNRK-----------TKIA 551
Query: 99 EIKKMKGQI---VVPVFYDVDPSDVRN 122
EIK +K I YD+ S RN
Sbjct: 552 EIKPVKALIRHRYRGKVYDIKLSSGRN 578
>DGAL_SALTY (P23905) D-galactose-binding periplasmic protein
precursor (GBP) (D-galactose/ D-glucose binding protein)
(GGBP)
Length = 332
Score = 28.9 bits (63), Expect = 5.6
Identities = 22/69 (31%), Positives = 34/69 (48%), Gaps = 14/69 (20%)
Query: 100 IKKMKGQIVVPVFYDVDPSDVRNQRGSYAEAF-----AKHENNFEGKIKVQEWRNGLLEA 154
I+K +GQ V VF++ +PS R SY +A+ +K +G + + W+
Sbjct: 100 IEKARGQNVPVVFFNKEPS--RKALDSYDKAYYVGTDSKESGVIQGDLIAKHWQ------ 151
Query: 155 ANYAGWDCN 163
AN GWD N
Sbjct: 152 AN-QGWDLN 159
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,238,062
Number of Sequences: 164201
Number of extensions: 820185
Number of successful extensions: 1890
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 1873
Number of HSP's gapped (non-prelim): 30
length of query: 166
length of database: 59,974,054
effective HSP length: 102
effective length of query: 64
effective length of database: 43,225,552
effective search space: 2766435328
effective search space used: 2766435328
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC134049.16