BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC133139.2 + phase: 0 /pseudo
(155 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NUB1_MOUSE (P54729) NEDD8 ultimate buster-1 (BS4 protein) 35 0.051
NUB1_HUMAN (Q9Y5A7) NEDD8 ultimate buster-1 (NY-REN-18 antigen) 35 0.087
BPEA_HUMAN (O94833) Bullous pemphigoid antigen 1, isoforms 6/9/1... 33 0.33
LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5) (Methy... 32 0.73
CYA1_HUMAN (Q08828) Adenylate cyclase, type I (EC 4.6.1.1) (ATP ... 30 1.6
PRTS_SERMA (P09489) Extracellular serine protease precursor (EC ... 29 3.6
GTH2_SCHPO (O59827) Glutathione S-transferase II (EC 2.5.1.18) (... 29 4.8
C1JB_BACTU (Q45716) Pesticidial crystal protein cry1Jb (Insectic... 29 4.8
BIOB_ARATH (P54967) Biotin synthase (EC 2.8.1.6) (Biotin synthet... 29 4.8
CYA1_BOVIN (P19754) Adenylate cyclase, type I (EC 4.6.1.1) (ATP ... 28 6.2
CU4B_HUMAN (Q13620) Cullin homolog 4B (CUL-4B) 28 6.2
YLH3_SCHPO (Q9HGQ3) Hypothetical transport protein C17D4.03c in ... 28 8.1
HS72_ANOAL (P41826) Heat shock protein 70 A2 28 8.1
HS71_ANOAL (P41825) Heat shock protein 70 A1 28 8.1
DPO3_STAAW (Q53665) DNA polymerase III polC-type (EC 2.7.7.7) (P... 28 8.1
DPO3_STAAN (P63982) DNA polymerase III polC-type (EC 2.7.7.7) (P... 28 8.1
DPO3_STAAM (P63981) DNA polymerase III polC-type (EC 2.7.7.7) (P... 28 8.1
C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb (Insectic... 28 8.1
ACE_CHICK (Q10751) Angiotensin-converting enzyme (EC 3.4.15.1) (... 28 8.1
>NUB1_MOUSE (P54729) NEDD8 ultimate buster-1 (BS4 protein)
Length = 614
Score = 35.4 bits (80), Expect = 0.051
Identities = 28/87 (32%), Positives = 39/87 (44%), Gaps = 4/87 (4%)
Query: 31 VRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYG-LNNINFLPSGYSMTQPSITHYR 89
+R D N H HI+ E+ + R EEK K+ L N+N L G + +
Sbjct: 396 LRACDGNVDHA--ATHISNRREELAQIRKEEKEKRRRRLENVNTL-RGMGYSTQAAKQAL 452
Query: 90 NQIRDWSDDVVKWVDDNPKKQWLQAHD 116
+Q R DD +K + NP WLQ D
Sbjct: 453 HQARGNLDDALKVLLSNPHMWWLQDAD 479
>NUB1_HUMAN (Q9Y5A7) NEDD8 ultimate buster-1 (NY-REN-18 antigen)
Length = 615
Score = 34.7 bits (78), Expect = 0.087
Identities = 31/88 (35%), Positives = 40/88 (45%), Gaps = 14/88 (15%)
Query: 31 VRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKK-YGLNNINFLPS-GYSMTQPSITHY 88
+R D N H HIT E+ + R EEK KK L NI FL GYS TH
Sbjct: 397 LRACDGNVDHA--ATHITNRREELAQIRKEEKEKKRRRLENIRFLKGMGYS------THA 448
Query: 89 RNQIRDWS----DDVVKWVDDNPKKQWL 112
Q+ + D+ +K + NP+ WL
Sbjct: 449 AQQVLHAASGNLDEALKILLSNPQMWWL 476
>BPEA_HUMAN (O94833) Bullous pemphigoid antigen 1, isoforms 6/9/10
(Trabeculin-beta) (Bullous pemphigoid antigen) (BPA)
(Hemidesmosomal plaque protein) (Dystonia musculorum
protein) (Dystonin)
Length = 5171
Score = 32.7 bits (73), Expect = 0.33
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Query: 16 DLGLISDRHESIKSVVRRLDSNWHHV-FCIRHITQLFEDFQEHRDEEK 62
D G+++D ++S+ V + H FC+ ++TQ F++FQE E K
Sbjct: 2616 DKGVVTDENKSLIQKVDMVTEQLHSKKFCLENMTQKFKEFQEVSKESK 2663
>LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5)
(Methylglyoxalase) (Aldoketomutase) (Glyoxalase I)
Length = 219
Score = 31.6 bits (70), Expect = 0.73
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Query: 79 SMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 120
S PS + R+Q R W D + K + D KK WL + H++G++
Sbjct: 82 SPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEEHEEGKK 126
>CYA1_HUMAN (Q08828) Adenylate cyclase, type I (EC 4.6.1.1) (ATP
pyrophosphate-lyase) (Ca(2+)/calmodulin activated
adenylyl cyclase) (Fragment)
Length = 839
Score = 30.4 bits (67), Expect = 1.6
Identities = 23/114 (20%), Positives = 51/114 (44%), Gaps = 9/114 (7%)
Query: 1 MSQAPDLRLWVTPQPDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQE---- 56
MS ++ L+ +G++ + LD N V C+R + ++ DF E
Sbjct: 571 MSNPRNMDLYYQSYSQVGVMFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDELMEK 630
Query: 57 --HRDEEKSKKYG---LNNINFLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDD 105
++D EK K G + + P+ + + SI+ + + + D++ ++ +D+
Sbjct: 631 DFYKDIEKIKTIGSTYMAAVGLAPTSGTKAKKSISSHLSTLADFAIEMFDVLDE 684
>PRTS_SERMA (P09489) Extracellular serine protease precursor (EC
3.4.21.-)
Length = 1045
Score = 29.3 bits (64), Expect = 3.6
Identities = 21/85 (24%), Positives = 33/85 (38%), Gaps = 15/85 (17%)
Query: 17 LGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPS 76
LGL D+ ++ WH + R + ++ D EK+K F+ S
Sbjct: 843 LGLYGDKRFGALALRAGGTYTWHRIDTSRSV-----NYGAQSDREKAKYNARTGQLFIES 897
Query: 77 GYSMTQPSI----------THYRNQ 91
GY T ++ THYRN+
Sbjct: 898 GYDWTSDAVNLEPFANLAYTHYRNE 922
>GTH2_SCHPO (O59827) Glutathione S-transferase II (EC 2.5.1.18)
(GST-II)
Length = 230
Score = 28.9 bits (63), Expect = 4.8
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 6/57 (10%)
Query: 24 HESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSM 80
HE + S V R + I+ + + ED + RD + KY + +++F+P Y++
Sbjct: 126 HEPVVSAVTRYRNE------IKRVLGVLEDILKDRDYLVANKYTIADLSFIPWNYNL 176
>C1JB_BACTU (Q45716) Pesticidial crystal protein cry1Jb
(Insecticidal delta-endotoxin CryIJ(b)) (Crystaline
entomocidal protoxin) (134 kDa crystal protein)
Length = 1170
Score = 28.9 bits (63), Expect = 4.8
Identities = 11/28 (39%), Positives = 18/28 (64%)
Query: 118 GRRWGHITTNISECFNNVLKGGPQSSNH 145
G RWG TTN+++ +N +K + S+H
Sbjct: 178 GERWGLTTTNVNDIYNRQVKRIHEYSDH 205
>BIOB_ARATH (P54967) Biotin synthase (EC 2.8.1.6) (Biotin
synthetase)
Length = 378
Score = 28.9 bits (63), Expect = 4.8
Identities = 18/56 (32%), Positives = 25/56 (44%)
Query: 94 DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 149
DWS D +K V D+P L Q R H + +C +K G S + + CP
Sbjct: 47 DWSRDEIKSVYDSPLLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCP 102
>CYA1_BOVIN (P19754) Adenylate cyclase, type I (EC 4.6.1.1) (ATP
pyrophosphate-lyase) (Ca(2+)/calmodulin activated
adenylyl cyclase)
Length = 1134
Score = 28.5 bits (62), Expect = 6.2
Identities = 22/114 (19%), Positives = 50/114 (43%), Gaps = 9/114 (7%)
Query: 1 MSQAPDLRLWVTPQPDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQE---- 56
MS ++ L+ +G++ + LD N V C+R + ++ DF E
Sbjct: 854 MSNPRNMDLYYQSYSQVGVMFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDELMDK 913
Query: 57 --HRDEEKSKKYG---LNNINFLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDD 105
++D EK K G + + P+ + + I+ + + + D++ ++ +D+
Sbjct: 914 DFYKDLEKIKTIGSTYMAAVGLAPTAGTKAKKCISSHLSTLADFAIEMFDVLDE 967
>CU4B_HUMAN (Q13620) Cullin homolog 4B (CUL-4B)
Length = 895
Score = 28.5 bits (62), Expect = 6.2
Identities = 18/98 (18%), Positives = 44/98 (44%), Gaps = 5/98 (5%)
Query: 44 IRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSMTQ----PSITHYRNQ-IRDWSDD 98
+R + + D Q ++D + + N + G + Q P H+ N+ + + +D
Sbjct: 350 LRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADR 409
Query: 99 VVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVL 136
++ ++D +K + ++ H+T + + NN+L
Sbjct: 410 LITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLL 447
>YLH3_SCHPO (Q9HGQ3) Hypothetical transport protein C17D4.03c in
chromosome I
Length = 732
Score = 28.1 bits (61), Expect = 8.1
Identities = 12/38 (31%), Positives = 23/38 (59%), Gaps = 3/38 (7%)
Query: 36 SNWHHVFCIRHITQ---LFEDFQEHRDEEKSKKYGLNN 70
S+ +H C+ + T +FE+F+E +D +++K G N
Sbjct: 486 SHSNHSMCLPNTTNDINIFEEFEEEKDNVEAQKMGYTN 523
>HS72_ANOAL (P41826) Heat shock protein 70 A2
Length = 640
Score = 28.1 bits (61), Expect = 8.1
Identities = 12/56 (21%), Positives = 26/56 (46%)
Query: 89 RNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSN 144
R ++D D+ ++W+D N + + Q + + + I + GGPQ ++
Sbjct: 565 RRTVQDRCDETLRWIDGNTMAEKEEYEHQMQELSRVCSPIMTKLHQQAAGGPQPTS 620
>HS71_ANOAL (P41825) Heat shock protein 70 A1
Length = 640
Score = 28.1 bits (61), Expect = 8.1
Identities = 12/56 (21%), Positives = 26/56 (46%)
Query: 89 RNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSN 144
R ++D D+ ++W+D N + + Q + + + I + GGPQ ++
Sbjct: 565 RRTVQDRCDETLRWIDGNTMAEKEEYEHQMQELSRVCSPIMTKLHQQAAGGPQPTS 620
>DPO3_STAAW (Q53665) DNA polymerase III polC-type (EC 2.7.7.7)
(PolIII)
Length = 1436
Score = 28.1 bits (61), Expect = 8.1
Identities = 20/82 (24%), Positives = 31/82 (37%), Gaps = 3/82 (3%)
Query: 44 IRHITQLFEDFQEHRDEEKSKKYGLNNINF-LPSGYSMTQ--PSITHYRNQIRDWSDDVV 100
IR + + D +E + K K + F L + S P+I Y Q DW +
Sbjct: 307 IRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAI 366
Query: 101 KWVDDNPKKQWLQAHDQGRRWG 122
D N + + AH + G
Sbjct: 367 AVTDHNVVQAFPDAHAAAEKHG 388
>DPO3_STAAN (P63982) DNA polymerase III polC-type (EC 2.7.7.7)
(PolIII)
Length = 1438
Score = 28.1 bits (61), Expect = 8.1
Identities = 20/82 (24%), Positives = 31/82 (37%), Gaps = 3/82 (3%)
Query: 44 IRHITQLFEDFQEHRDEEKSKKYGLNNINF-LPSGYSMTQ--PSITHYRNQIRDWSDDVV 100
IR + + D +E + K K + F L + S P+I Y Q DW +
Sbjct: 309 IRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAI 368
Query: 101 KWVDDNPKKQWLQAHDQGRRWG 122
D N + + AH + G
Sbjct: 369 AVTDHNVVQAFPDAHAAAEKHG 390
>DPO3_STAAM (P63981) DNA polymerase III polC-type (EC 2.7.7.7)
(PolIII)
Length = 1438
Score = 28.1 bits (61), Expect = 8.1
Identities = 20/82 (24%), Positives = 31/82 (37%), Gaps = 3/82 (3%)
Query: 44 IRHITQLFEDFQEHRDEEKSKKYGLNNINF-LPSGYSMTQ--PSITHYRNQIRDWSDDVV 100
IR + + D +E + K K + F L + S P+I Y Q DW +
Sbjct: 309 IRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAI 368
Query: 101 KWVDDNPKKQWLQAHDQGRRWG 122
D N + + AH + G
Sbjct: 369 AVTDHNVVQAFPDAHAAAEKHG 390
>C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb
(Insecticidal delta-endotoxin CryIC(b)) (Crystaline
entomocidal protoxin) (133 kDa crystal protein)
Length = 1176
Score = 28.1 bits (61), Expect = 8.1
Identities = 10/28 (35%), Positives = 18/28 (63%)
Query: 118 GRRWGHITTNISECFNNVLKGGPQSSNH 145
G RWG T N++E +N +++ + +NH
Sbjct: 178 GARWGLTTINVNENYNRLIRHIDEYANH 205
>ACE_CHICK (Q10751) Angiotensin-converting enzyme (EC 3.4.15.1)
(Dipeptidyl carboxypeptidase I) (Kininase II) (Fragment)
Length = 1193
Score = 28.1 bits (61), Expect = 8.1
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 2/59 (3%)
Query: 24 HESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSMTQ 82
HE+I V+ S H+ I + Q+ E+ E D L+ I FLP GY M Q
Sbjct: 913 HEAIGDVMALSVSTPKHLHSINLLDQVTEN--EESDINYLMSIALDKIAFLPFGYLMDQ 969
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,025,441
Number of Sequences: 164201
Number of extensions: 855412
Number of successful extensions: 2077
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 2069
Number of HSP's gapped (non-prelim): 21
length of query: 155
length of database: 59,974,054
effective HSP length: 101
effective length of query: 54
effective length of database: 43,389,753
effective search space: 2343046662
effective search space used: 2343046662
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC133139.2