BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.12 + phase: 0
(162 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DFRA_PETHY (P14720) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 92 5e-19
DFRA_LYCES (P51107) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 91 1e-18
DFRA_GERHY (P51105) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 89 3e-18
DFRA_CALCH (P51103) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 89 4e-18
DFRA_DIACA (P51104) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 88 1e-17
DFRA_ANTMA (P14721) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 85 6e-17
DFRA_ARATH (P51102) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 82 4e-16
DFRA_VITVI (P51110) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 79 3e-15
BAN_ARATH (Q9SEV0) Leucoanthocyanidin reductase (EC 1.-.-.-) (LA... 76 3e-14
DFRA_MAIZE (P51108) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 76 4e-14
DFRA_HORVU (P51106) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 75 6e-14
DFRA_MEDSA (P51109) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 63 3e-10
NSDL_MOUSE (Q9R1J0) NAD(P)-dependent steroid dehydrogenase (EC 1... 48 1e-05
DFRA_SYNY3 (P73212) Putative dihydroflavonol-4-reductase (EC 1.1... 46 3e-05
HLDD_PHOLL (Q7MY46) ADP-L-glycero-D-manno-heptose-6-epimerase (E... 42 8e-04
NSDL_HUMAN (Q15738) NAD(P)-dependent steroid dehydrogenase (EC 1... 41 0.001
HLDD_SHIFL (Q83PP2) ADP-L-glycero-D-manno-heptose-6-epimerase (E... 40 0.002
HLDD_SALTY (P67912) ADP-L-glycero-D-manno-heptose-6-epimerase (E... 40 0.002
HLDD_SALTI (P67913) ADP-L-glycero-D-manno-heptose-6-epimerase (E... 40 0.002
HLDD_KLEPN (Q9XCA1) ADP-L-glycero-D-manno-heptose-6-epimerase (E... 40 0.002
>DFRA_PETHY (P14720) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 380
Score = 92.0 bits (227), Expect = 5e-19
Identities = 45/80 (56%), Positives = 58/80 (72%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AG+IGS LV +LLE+GY VHAT+R+ +++ KV L P A+T L L++AD+
Sbjct: 18 VCVTGAAGFIGSWLVMRLLERGYNVHATVRDPENKKKVKHLLELPKADTNLTLWKADLTV 77
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AIQGC+ VFHVATP
Sbjct: 78 EGSFDEAIQGCQGVFHVATP 97
>DFRA_LYCES (P51107) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 379
Score = 90.5 bits (223), Expect = 1e-18
Identities = 45/80 (56%), Positives = 58/80 (72%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
V VTGGAG+IGS LV +LLE+GY VHAT+R+ +++ KV L P A+T L L++AD+
Sbjct: 20 VWVTGGAGFIGSWLVMRLLERGYNVHATVRDPENQKKVKHLLELPKADTNLTLWKADLAV 79
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AIQGC+ VFHVATP
Sbjct: 80 EGSFDEAIQGCQGVFHVATP 99
>DFRA_GERHY (P51105) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 366
Score = 89.4 bits (220), Expect = 3e-18
Identities = 45/80 (56%), Positives = 55/80 (68%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AG+IGS LV +LLE+GY VHAT+R+ D KV L P A T L L++AD+ +
Sbjct: 9 VCVTGAAGFIGSWLVMRLLERGYVVHATVRDPGDLKKVKHLLELPKAQTNLKLWKADLTQ 68
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AIQGC VFH+ATP
Sbjct: 69 EGSFDEAIQGCHGVFHLATP 88
>DFRA_CALCH (P51103) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 364
Score = 89.0 bits (219), Expect = 4e-18
Identities = 45/80 (56%), Positives = 54/80 (67%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AG+IGS LV +LLE+GY V AT+RN D KV L P A T L L++AD+ +
Sbjct: 9 VCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLTLWKADLTQ 68
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFHVATP
Sbjct: 69 EGSFDEAIEGCHGVFHVATP 88
>DFRA_DIACA (P51104) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 360
Score = 87.8 bits (216), Expect = 1e-17
Identities = 40/80 (50%), Positives = 56/80 (70%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+IGS L+ +LLE+GYTV AT+R+ + KV L P+A T L L++AD+++
Sbjct: 25 VCVTGASGFIGSWLIMRLLERGYTVRATVRDPDNTKKVQHLLDLPNAKTNLTLWKADLHE 84
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F A+ GC VFH+ATP
Sbjct: 85 EGSFDAAVDGCTGVFHIATP 104
>DFRA_ANTMA (P14721) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 446
Score = 85.1 bits (209), Expect = 6e-17
Identities = 44/79 (55%), Positives = 55/79 (68%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AG+IGS LV +LLE+GYTV AT+R+ + KV L P A+T L L++AD+
Sbjct: 20 VCVTGAAGFIGSWLVMRLLERGYTVRATVRDPGNMKKVKHLIELPKADTNLTLWKADMTV 79
Query: 67 PDDFGTAIQGCEFVFHVAT 85
F AIQGCE VFH+AT
Sbjct: 80 EGSFDEAIQGCEGVFHLAT 98
>DFRA_ARATH (P51102) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase) (TRANSPARENT
TESTA 3 protein)
Length = 382
Score = 82.4 bits (202), Expect = 4e-16
Identities = 41/80 (51%), Positives = 55/80 (68%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+IGS LV +LLE+GY V AT+R+ + KV L P+A T L L++AD+ +
Sbjct: 8 VCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWKADLSE 67
Query: 67 PDDFGTAIQGCEFVFHVATP 86
+ AI GC+ VFHVATP
Sbjct: 68 EGSYDDAINGCDGVFHVATP 87
>DFRA_VITVI (P51110) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 337
Score = 79.3 bits (194), Expect = 3e-15
Identities = 41/80 (51%), Positives = 52/80 (64%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+IGS LV +LLE+ TV AT+R+ + KV L P A T L L++AD+
Sbjct: 8 VCVTGASGFIGSWLVMRLLERRLTVRATVRDPTNVKKVKHLLDLPKAETHLTLWKADLAD 67
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFHVATP
Sbjct: 68 EGSFDEAIKGCTGVFHVATP 87
>BAN_ARATH (Q9SEV0) Leucoanthocyanidin reductase (EC 1.-.-.-)
(LAR) (BANYULS) (Anthocyanin spotted testa) (ast)
Length = 340
Score = 76.3 bits (186), Expect = 3e-14
Identities = 35/81 (43%), Positives = 53/81 (65%), Gaps = 1/81 (1%)
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIY 65
K CV GG G + S+L+K LL+ GY V+ T+R+ ++E K++ LR L +F+AD+
Sbjct: 12 KACVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKLQELGD-LKIFKADLT 70
Query: 66 KPDDFGTAIQGCEFVFHVATP 86
D F ++ GCE++FHVATP
Sbjct: 71 DEDSFESSFSGCEYIFHVATP 91
>DFRA_MAIZE (P51108) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 357
Score = 75.9 bits (185), Expect = 4e-14
Identities = 39/80 (48%), Positives = 52/80 (64%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
V VTG +G++GS LV KLL+ GYTV AT+R+ + K L P A RL +++AD+ +
Sbjct: 13 VLVTGASGFVGSWLVMKLLQAGYTVRATVRDPANVGKTKPLMDLPGATERLSIWKADLAE 72
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFHVATP
Sbjct: 73 EGSFHDAIRGCTGVFHVATP 92
>DFRA_HORVU (P51106) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase)
Length = 354
Score = 75.1 bits (183), Expect = 6e-14
Identities = 39/80 (48%), Positives = 51/80 (63%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
V VTG +G++GS LV KLL+ GYTV AT+R+ + K L P A RL +++AD+ +
Sbjct: 8 VVVTGASGFVGSWLVMKLLQAGYTVRATVRDPANVEKTKPLLELPGAKERLSIWKADLSE 67
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI GC VFHVATP
Sbjct: 68 DGSFNEAIAGCTGVFHVATP 87
>DFRA_MEDSA (P51109) Dihydroflavonol-4-reductase (EC 1.1.1.219)
(DFR) (Dihydrokaempferol 4-reductase) (Fragment)
Length = 217
Score = 63.2 bits (152), Expect = 3e-10
Identities = 33/70 (47%), Positives = 47/70 (67%)
Query: 17 GSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYKPDDFGTAIQG 76
GS LV +L+E GY V AT+R+ ++ KVS L P A ++L +++AD+ + F AI+G
Sbjct: 1 GSWLVMRLMEPGYMVRATVRDPENLKKVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKG 60
Query: 77 CEFVFHVATP 86
C VFHVATP
Sbjct: 61 CTGVFHVATP 70
>NSDL_MOUSE (Q9R1J0) NAD(P)-dependent steroid dehydrogenase (EC
1.1.1.-)
Length = 362
Score = 47.8 bits (112), Expect = 1e-05
Identities = 29/97 (29%), Positives = 49/97 (49%), Gaps = 12/97 (12%)
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIY 65
K V GG+G++G +V++LLE+GYTV+ +GF N R+ F D+
Sbjct: 28 KCTVIGGSGFLGQHMVEQLLERGYTVNV----------FDIHQGFD--NPRVQFFIGDLC 75
Query: 66 KPDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILII 102
D A++G VFH A+P + + ++ ++ I
Sbjct: 76 NQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFI 112
>DFRA_SYNY3 (P73212) Putative dihydroflavonol-4-reductase (EC
1.1.1.219) (DFR) (Dihydrokaempferol 4-reductase)
Length = 343
Score = 46.2 bits (108), Expect = 3e-05
Identities = 32/87 (36%), Positives = 48/87 (54%), Gaps = 10/87 (11%)
Query: 9 VTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYKPD 68
VTGG G++G+ LV+ LLE+GY V A +R S+ L+ P L + D+++
Sbjct: 15 VTGGTGFVGANLVRHLLEQGYQVRALVR---ASSRPDNLQNLPIDWVVGDLNDGDLHQ-- 69
Query: 69 DFGTAIQGCEFVFHVATPY-LHQTDSQ 94
+QGC+ +FHVA Y L Q D +
Sbjct: 70 ----QMQGCQGLFHVAAHYSLWQKDRE 92
>HLDD_PHOLL (Q7MY46) ADP-L-glycero-D-manno-heptose-6-epimerase (EC
5.1.3.20)
(ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
(ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
6-epimerase) (AGME)
Length = 312
Score = 41.6 bits (96), Expect = 8e-04
Identities = 28/66 (42%), Positives = 36/66 (54%), Gaps = 1/66 (1%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHAN-TRLVLFEADIY 65
+ VTGGAG+IGS +VK L ++GY + NLKD +K L A+ F A I
Sbjct: 2 IIVTGGAGFIGSNIVKALNDEGYKDILVVDNLKDGTKFINLADLDIADYMDKEEFIASIL 61
Query: 66 KPDDFG 71
DDFG
Sbjct: 62 AGDDFG 67
>NSDL_HUMAN (Q15738) NAD(P)-dependent steroid dehydrogenase (EC
1.1.1.-) (H105e3 protein)
Length = 373
Score = 41.2 bits (95), Expect = 0.001
Identities = 26/84 (30%), Positives = 41/84 (47%), Gaps = 14/84 (16%)
Query: 3 RRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEA 62
+RC V GG+G++G +V++LL +GY V+ +GF + R F
Sbjct: 38 KRCTVI--GGSGFLGQHMVEQLLARGYAVNV----------FDIQQGFDNPQVR--FFLG 83
Query: 63 DIYKPDDFGTAIQGCEFVFHVATP 86
D+ D A++G VFH A+P
Sbjct: 84 DLCSRQDLYPALKGVNTVFHCASP 107
>HLDD_SHIFL (Q83PP2) ADP-L-glycero-D-manno-heptose-6-epimerase (EC
5.1.3.20)
(ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
(ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
6-epimerase) (AGME)
Length = 310
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/37 (54%), Positives = 26/37 (70%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK 43
+ VTGGAG+IGS +VK L +KG T + NLKD +K
Sbjct: 2 IIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTK 38
>HLDD_SALTY (P67912) ADP-L-glycero-D-manno-heptose-6-epimerase (EC
5.1.3.20)
(ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
(ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
6-epimerase) (AGME)
Length = 310
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/37 (54%), Positives = 26/37 (70%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK 43
+ VTGGAG+IGS +VK L +KG T + NLKD +K
Sbjct: 2 IIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTK 38
>HLDD_SALTI (P67913) ADP-L-glycero-D-manno-heptose-6-epimerase (EC
5.1.3.20)
(ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
(ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
6-epimerase) (AGME)
Length = 310
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/37 (54%), Positives = 26/37 (70%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK 43
+ VTGGAG+IGS +VK L +KG T + NLKD +K
Sbjct: 2 IIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTK 38
>HLDD_KLEPN (Q9XCA1) ADP-L-glycero-D-manno-heptose-6-epimerase (EC
5.1.3.20)
(ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
(ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
6-epimerase) (AGME)
Length = 310
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/37 (54%), Positives = 26/37 (70%)
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK 43
+ VTGGAG+IGS +VK L +KG T + NLKD +K
Sbjct: 2 IIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTK 38
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.142 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,613,707
Number of Sequences: 164201
Number of extensions: 856825
Number of successful extensions: 2300
Number of sequences better than 10.0: 181
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 2155
Number of HSP's gapped (non-prelim): 183
length of query: 162
length of database: 59,974,054
effective HSP length: 102
effective length of query: 60
effective length of database: 43,225,552
effective search space: 2593533120
effective search space used: 2593533120
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC129091.12