BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.6 - phase: 0 /pseudo
(322 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LIPB_PORGI (Q7MUY1) Lipoyltransferase (EC 2.3.1.-) (Lipoyl-[acyl... 72 2e-12
PHNP_ECOLI (P16692) PhnP protein 36 0.11
SPT1_MOUSE (Q9R097) Kunitz-type protease inhibitor 1 precursor (... 35 0.33
PAG1_SHEEP (Q28755) Pregnancy-associated glycoprotein 1 precurso... 33 0.73
CTE2_XENLA (Q805F2) Cathepsin E2 precursor (EC 3.4.23.34) 33 0.73
Y020_PYRFU (P81414) Hypothetical UPF0173 protein PF0020 33 0.96
RNZ_STAEP (Q8CSG7) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 33 0.96
RNZ_BRAJA (Q89NF8) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 33 0.96
FND3_HUMAN (Q9Y2H6) Fibronectin type III domain containing prote... 33 0.96
RNZ_LISIN (Q92A38) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 33 1.2
CTE1_XENLA (Q805F3) Cathepsin E1 precursor (EC 3.4.23.34) 33 1.2
RNZ_METKA (Q8TWK0) Zinc phosphodiesterase elaC (EC 3.1.4.-) (ZiPD) 31 3.6
PGS2_RAT (Q01129) Decorin precursor (Bone proteoglycan II) (PG-S... 31 3.6
PGS2_HUMAN (P07585) Decorin precursor (Bone proteoglycan II) (PG... 31 3.6
RNZ_CLOAB (Q97IQ6) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 31 4.7
RNZ_LACPL (Q88VG6) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 30 6.2
DHNO_AGRT7 (P00386) D-nopaline dehydrogenase (EC 1.5.1.19) (Nopa... 30 6.2
>LIPB_PORGI (Q7MUY1) Lipoyltransferase (EC 2.3.1.-)
(Lipoyl-[acyl-carrier protein]-protein
-N-lipoyltransferase) (Lipoate-protein ligase B)
Length = 492
Score = 72.0 bits (175), Expect = 2e-12
Identities = 65/211 (30%), Positives = 98/211 (45%), Gaps = 38/211 (18%)
Query: 19 IFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYCGSGSNHN 78
I LG+G S+ VP V C C VC E + R TS+LI +G
Sbjct: 4 ILLGSGTSTGVPEVGC-------HCRVCRS------EDRHDKRTRTSLLI-ITDAGKR-- 47
Query: 79 YILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTNDIDPT 138
ILID FR+ L I +D+++LTHEH D V GLDD+R +
Sbjct: 48 -ILIDCSPDFRQQAL----FAGIDSLDAVLLTHEHFDHVGGLDDLRTICWHR------EL 96
Query: 139 PIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIITDDCNQPFFASGLKFTPLP 198
+Y Q+ +DSI ++ Y+ +K G + ++ ++ + PF + L PL
Sbjct: 97 AVYAEQNVLDSIRDRLHYVFRKNPYPGTPLLKLCEV-------KPDMPFQVADLTVEPLR 149
Query: 199 VMHGEDYICLGFLFGEKSRVAYISDVSRIPA 229
+MHG I LG+ GE +A+++D+ I A
Sbjct: 150 IMHGRLPI-LGYKIGE---MAFLTDMKDIAA 176
>PHNP_ECOLI (P16692) PhnP protein
Length = 252
Score = 36.2 bits (82), Expect = 0.11
Identities = 52/221 (23%), Positives = 82/221 (36%), Gaps = 60/221 (27%)
Query: 18 LIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNT-SMLIDYCGSGSN 76
L GTG + VP C C+ CA++ ++P YR S ++ + N
Sbjct: 5 LTLTGTGGAQGVPAWGC-------ECAACARA-----RRSPQYRRQPCSGVVKF-----N 47
Query: 77 HNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVR-----AVQPFSP 131
LID G + RW +LTH H D V GL +R + + P
Sbjct: 48 DAITLIDAG--LHDLADRWSPG----SFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGP 101
Query: 132 TNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIITDDCNQPFFASG 191
++ ++ +D P++V F G
Sbjct: 102 PDEQGCDDLFKHPGLLDFSHTVEPFVV----------------------------FDLQG 133
Query: 192 LKFTPLPVMHGEDYICLGFLFGEK-SRVAYISDVSRIPAST 231
L+ TPLP+ H + + G+L SRVA++SD + +P T
Sbjct: 134 LQVTPLPLNHSK--LTFGYLLETAHSRVAWLSDTAGLPEKT 172
>SPT1_MOUSE (Q9R097) Kunitz-type protease inhibitor 1 precursor
(Hepatocyte growth factor activator inhibitor type 1)
(HAI-1)
Length = 507
Score = 34.7 bits (78), Expect = 0.33
Identities = 34/140 (24%), Positives = 56/140 (39%), Gaps = 25/140 (17%)
Query: 78 NYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTNDIDP 137
NY+ ++ +++RE R F RIP+I +G+D +Q N+ D
Sbjct: 133 NYLTQELYRSYRELRTRGFGGSRIPRI------------WMGIDLKVQLQKPLVLNEADN 180
Query: 138 TPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIR----RVAQMAWNIITDDCNQPFFASGLK 193
T +L Q D V+ +RK +E+ + + + D +QP + L
Sbjct: 181 TDWHLLQGDSD---------VRVERKRPEEVELWGLKEGTYLFQLTRTDSDQPEETANLT 231
Query: 194 FTPLPVMHGEDYICLGFLFG 213
T L M EDY + G
Sbjct: 232 ITVLTAMQTEDYCLASYKVG 251
>PAG1_SHEEP (Q28755) Pregnancy-associated glycoprotein 1 precursor
(EC 3.4.23.-) (PAG 1)
Length = 382
Score = 33.5 bits (75), Expect = 0.73
Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
Query: 10 GTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLID 69
GTPP E ++F +VP++ CL +P+ PCS + ++ +R
Sbjct: 78 GTPPQEFQVVFDTGSSDLLVPSINCL-SPTKRPCS---KQDKFKHHQSSTFRFTNDTFRI 133
Query: 70 YCGSGSNHNYILID 83
Y GSG+ ++ D
Sbjct: 134 YFGSGTMRGFVAHD 147
>CTE2_XENLA (Q805F2) Cathepsin E2 precursor (EC 3.4.23.34)
Length = 397
Score = 33.5 bits (75), Expect = 0.73
Identities = 27/75 (36%), Positives = 37/75 (49%), Gaps = 9/75 (12%)
Query: 10 GTPPSESALIFLGTGCSSM-VPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLI 68
GTPP +IF TG S++ VP+V C I+P+ CAQ P+ + Y N +
Sbjct: 81 GTPPQNFTVIF-DTGSSNLWVPSVYC-ISPA------CAQHNRFQPQLSSTYESNGNNFS 132
Query: 69 DYCGSGSNHNYILID 83
G+GS I ID
Sbjct: 133 LQYGTGSLSGVIGID 147
>Y020_PYRFU (P81414) Hypothetical UPF0173 protein PF0020
Length = 210
Score = 33.1 bits (74), Expect = 0.96
Identities = 39/149 (26%), Positives = 59/149 (39%), Gaps = 35/149 (23%)
Query: 100 RIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQ 159
RI K+D I++THEH D + S I P +YL S D + +
Sbjct: 32 RIGKVDYILVTHEHMD---HYGKTPLIAKLSDAEVIGPKTVYLMAIS-DGLTK------V 81
Query: 160 KQRKEGQEIRRVAQMAWNIITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVA 219
++ + GQEI + D + FF H LG+L RVA
Sbjct: 82 REIEVGQEIE---------LGDIRVRAFFTE----------HPTSQYPLGYLIEGSKRVA 122
Query: 220 YISDVSRIPASTEYVISKSGAGQLDLLIL 248
++ D PA TE G++D+L++
Sbjct: 123 HLGDTYYSPAFTEL------RGKVDVLLV 145
>RNZ_STAEP (Q8CSG7) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 306
Score = 33.1 bits (74), Expect = 0.96
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 2/49 (4%)
Query: 81 LIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPF 129
L DVG+ + +LR + ++ KID I +TH H D + GL + + F
Sbjct: 35 LFDVGEGTQHQILRHSI--KLGKIDHIFITHMHGDHIFGLPGLLTSRSF 81
>RNZ_BRAJA (Q89NF8) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 301
Score = 33.1 bits (74), Expect = 0.96
Identities = 25/87 (28%), Positives = 44/87 (49%), Gaps = 11/87 (12%)
Query: 49 SLSIPPEKNPNYRCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSII 108
S S+P + R + ++L++ G ILID G+ + +LR R ++D I+
Sbjct: 10 SASVPSAE----RNHPALLVEAAGKR-----ILIDCGEGTQRQLLRSGAGFR--RLDRIL 58
Query: 109 LTHEHADAVLGLDDVRAVQPFSPTNDI 135
LTH H D VLG+ + + ++D+
Sbjct: 59 LTHAHLDHVLGIPGLFSTLGLRQSSDV 85
>FND3_HUMAN (Q9Y2H6) Fibronectin type III domain containing protein
3
Length = 1134
Score = 33.1 bits (74), Expect = 0.96
Identities = 25/72 (34%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
Query: 17 ALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDY---CGS 73
AL +G G S V V C+ PS P C Q +S +NP+Y +T + I + C
Sbjct: 767 ALSVVGAGPFSEV--VACVTPPSVPGIVTCLQEISDDEIENPHYSPSTCLAISWEKPCDH 824
Query: 74 GSNHNYILIDVG 85
GS ID G
Sbjct: 825 GSEILAYSIDFG 836
>RNZ_LISIN (Q92A38) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 306
Score = 32.7 bits (73), Expect = 1.2
Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 2/49 (4%)
Query: 81 LIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPF 129
L D G+ + VLR H ++ K++ I +TH H D + GL + + + F
Sbjct: 35 LFDCGEATQHQVLR--SHIKLSKLEKIFITHMHGDHIFGLPGLLSSRSF 81
>CTE1_XENLA (Q805F3) Cathepsin E1 precursor (EC 3.4.23.34)
Length = 397
Score = 32.7 bits (73), Expect = 1.2
Identities = 25/75 (33%), Positives = 34/75 (45%), Gaps = 9/75 (12%)
Query: 10 GTPPSESALIFLGTGCSSM-VPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLI 68
GTPP +IF TG S++ VP+V C+ CAQ P+ + Y N +
Sbjct: 81 GTPPQNFTVIF-DTGSSNLWVPSVYCI-------SQACAQHDRFQPQLSSTYESNGNNFS 132
Query: 69 DYCGSGSNHNYILID 83
G+GS I ID
Sbjct: 133 LQYGTGSLSGVIGID 147
>RNZ_METKA (Q8TWK0) Zinc phosphodiesterase elaC (EC 3.1.4.-) (ZiPD)
Length = 279
Score = 31.2 bits (69), Expect = 3.6
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 7/60 (11%)
Query: 61 RCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGL 120
R + +L+++ G+ +LID G+ + + V I +D+++LTH H D V GL
Sbjct: 21 RSHPGLLVEFSGTK-----LLIDCGEGTQRRAMEQGV--TIHDVDAVLLTHHHVDHVAGL 73
>PGS2_RAT (Q01129) Decorin precursor (Bone proteoglycan II) (PG-S2)
(PG40) (Dermatan sulfate proteoglycan-II) (DSPG)
Length = 354
Score = 31.2 bits (69), Expect = 3.6
Identities = 20/72 (27%), Positives = 35/72 (47%), Gaps = 5/72 (6%)
Query: 140 IYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWN-----IITDDCNQPFFASGLKF 194
+YLS++ + + EK P +Q+ R EI ++ + +N I+ + P SG++
Sbjct: 129 LYLSKNHLKELPEKLPKTLQELRLHDNEITKLKKSVFNGLNRMIVIELGGNPLKNSGIEN 188
Query: 195 TPLPVMHGEDYI 206
L M G YI
Sbjct: 189 GALQGMKGLGYI 200
>PGS2_HUMAN (P07585) Decorin precursor (Bone proteoglycan II)
(PG-S2) (PG40)
Length = 359
Score = 31.2 bits (69), Expect = 3.6
Identities = 20/82 (24%), Positives = 41/82 (49%), Gaps = 5/82 (6%)
Query: 117 VLGLDDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAW 176
+L + + V P + T + +YLS++ + + EK P +Q+ R EI +V ++ +
Sbjct: 111 ILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTF 170
Query: 177 N-----IITDDCNQPFFASGLK 193
N I+ + P +SG++
Sbjct: 171 NGLNQMIVIELGTNPLKSSGIE 192
>RNZ_CLOAB (Q97IQ6) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 309
Score = 30.8 bits (68), Expect = 4.7
Identities = 26/77 (33%), Positives = 37/77 (47%), Gaps = 9/77 (11%)
Query: 64 TSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDV 123
TS+LI Y N ILID G+ + ++ + ID I TH HAD V+GL +
Sbjct: 21 TSLLISY-----NGRKILIDCGEGTQVSMKE--IAWGFKDIDVICFTHYHADHVMGLTGL 73
Query: 124 RAVQPFSPTNDIDPTPI 140
+ + + IDP I
Sbjct: 74 --LLTIANSGRIDPLTI 88
>RNZ_LACPL (Q88VG6) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 311
Score = 30.4 bits (67), Expect = 6.2
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Query: 81 LIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTNDIDPTPI 140
L DVG+ + +LR + R KI I +TH H D + GL + + + F + +P I
Sbjct: 35 LFDVGEGTQHQILRTTLKPR--KIAKIFITHLHGDHIFGLPGLLSSRSFQGGD--EPLTI 90
Query: 141 Y 141
Y
Sbjct: 91 Y 91
>DHNO_AGRT7 (P00386) D-nopaline dehydrogenase (EC 1.5.1.19)
(Nopaline synthase)
Length = 413
Score = 30.4 bits (67), Expect = 6.2
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 2/73 (2%)
Query: 8 DDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPP-CSVCAQSL-SIPPEKNPNYRCNTS 65
D+ TP +E F G G ++ V NV I+ + C L S+ E N Y +
Sbjct: 255 DERTPRAEKGFFFYGEGSNTYVCNVQEQIDHERRKVAAACGLRLNSLLQECNDEYDTDYE 314
Query: 66 MLIDYCGSGSNHN 78
L +YC + S HN
Sbjct: 315 TLREYCLAPSPHN 327
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.332 0.145 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,479,391
Number of Sequences: 164201
Number of extensions: 1642745
Number of successful extensions: 4276
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 4266
Number of HSP's gapped (non-prelim): 18
length of query: 322
length of database: 59,974,054
effective HSP length: 110
effective length of query: 212
effective length of database: 41,911,944
effective search space: 8885332128
effective search space used: 8885332128
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 66 (30.0 bits)
Medicago: description of AC127428.6