BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127169.2 - phase: 0
(270 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YSM6_CAEEL (Q10126) Hypothetical protein F52C9.6 in chromosome III 62 1e-09
RTJK_DROME (P21328) RNA-directed DNA polymerase from mobile elem... 42 0.002
RTJK_DROFU (P21329) RNA-directed DNA polymerase from mobile elem... 38 0.030
PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type... 37 0.067
RUV2_XENLA (Q9DE27) RuvB-like 2 (EC 3.6.1.-) (Reptin) 32 1.6
RUV2_MOUSE (Q9WTM5) RuvB-like 2 (EC 3.6.1.-) (p47 protein) 32 1.6
RUV2_HUMAN (Q9Y230) RuvB-like 2 (EC 3.6.1.-) (48-kDa TATA box-bi... 32 1.6
YMJ3_YEAST (Q04500) Hypothetical 103.0 kDa protein in RAD10-PRS4... 32 2.1
RUV2_BRARE (P83571) RuvB-like 2 (EC 3.6.1.-) (Reptin) (zReptin) 31 3.7
PCF1_MOUSE (P59114) Phosphorylated CTD interacting factor 1 30 4.8
YCF2_MESVI (Q9MUP8) Protein ycf2 (RF2) 30 6.2
TAF1_DROME (P51123) Transcription initiation factor TFIID subuni... 30 6.2
>YSM6_CAEEL (Q10126) Hypothetical protein F52C9.6 in chromosome III
Length = 279
Score = 62.4 bits (150), Expect = 1e-09
Identities = 55/255 (21%), Positives = 102/255 (39%), Gaps = 26/255 (10%)
Query: 25 FADDVVLVGESREEVNGRLETWRQALEAYGFRLSRSKTEYIECNFS-------GRRSRST 77
FADD+VL+ + L+ Q G ++ KT+ + + G S +T
Sbjct: 13 FADDIVLIANHPNTASKMLQELVQKCSEVGLEINTGKTKVLRNRLADPSKVYFGSPSSTT 72
Query: 78 LEVKVEDHIIPQVTRSKYLGSFVQNDGEIEADVSHRIQAGWLKW---RRDSDVLCDKKVP 134
V+++I YLG + + ++ R +A W + + +D + DKK+
Sbjct: 73 QLDDVDEYI--------YLGRQINAHNNLMPEIHRRRRAAWAAFNGSKNTTDSITDKKIR 124
Query: 135 LKLKGKFYRPAFRPALMYGRECWAVKSQHENQVSVA----EMRMLRWMSSKTRHDRTRND 190
+ L + PAL YG E W +V + E R++ ++ R +
Sbjct: 125 VNL----FDSIVLPALTYGSEAWTFNKALSERVRITHASLERRLVGITLTQQRERDLHRE 180
Query: 191 TIRKRVEVAPIVEKLVENRLRWFGHVERRPVDAVVRRVYQMEDSQVKRGRGRPRKTIRET 250
IR +V + + + +L W GHV RR + + KR GR ++
Sbjct: 181 DIRVMSQVRDPLNFVKKRKLGWAGHVARRKDGRWTTLMTEWRPWNWKRYVGRTPMRWTDS 240
Query: 251 IRKDLEVNELDPNLV 265
+RK++ + D ++
Sbjct: 241 LRKEITTRDADGEVI 255
>RTJK_DROME (P21328) RNA-directed DNA polymerase from mobile element
jockey (EC 2.7.7.49) (Reverse transcriptase)
Length = 916
Score = 42.0 bits (97), Expect = 0.002
Identities = 46/216 (21%), Positives = 96/216 (44%), Gaps = 16/216 (7%)
Query: 1 MFNCFNRNMSVTEHIQELAPRCML---FADDVVLVGESREEVNGR------LETWRQALE 51
+++ F +M + E+ +L +ADD ++ +S+ + L+ ++Q E
Sbjct: 652 LYSVFASDMPTHTPVTEVDEEDVLIATYADDTAVLTKSKSILAATSGLQEYLDAFQQWAE 711
Query: 52 AYGFRLSRSKTEYIECNFSGRRSRSTLEVKVEDHIIPQVTRSKYLGSFVQNDGEIEADVS 111
+ R++ K + R+ S V + +I KYLG + ++
Sbjct: 712 NWNVRINAEKCANVTF---ANRTGSCPGVSLNGRLIRHHQAYKYLGITLDRKLTFSRHIT 768
Query: 112 HRIQAGWLKWRRDSDVLCDK-KVPLKLKGKFYRPAFRPALMYGRECWAVKSQ-HENQVSV 169
+ QA K R S ++ + K+ L K Y+ P L YG + + + ++ H N++ +
Sbjct: 769 NIQQAFRTKVARMSWLIAPRNKLSLGCKVNIYKSILAPCLFYGLQVYGIAAKSHLNKIRI 828
Query: 170 AEMRMLRWMSSKTRHDRTRNDTIRKRVEVAPIVEKL 205
+ + LR +S + RTR+ I + ++V + +KL
Sbjct: 829 LQAKTLRRISGAPWYMRTRD--IERDLKVPKLGDKL 862
>RTJK_DROFU (P21329) RNA-directed DNA polymerase from mobile element
jockey (EC 2.7.7.49) (Reverse transcriptase)
Length = 916
Score = 37.7 bits (86), Expect = 0.030
Identities = 53/256 (20%), Positives = 103/256 (39%), Gaps = 18/256 (7%)
Query: 1 MFNCFNRNMSVTEHIQELAPRCML---FADDVVLVGESR--EEVNGRLETWRQALEAYGF 55
+++ F +M + LA +L +ADD+ ++ +S E L+ + A + +
Sbjct: 652 LYSIFTADMPNQNAVTGLAEGEVLIATYADDIAVLTKSTCIVEATDALQEYLDAFQEWAV 711
Query: 56 RLSRSKTEYIECNFSGRRS-RSTLEVKVEDHIIPQVTRSKYLGSFVQNDGEIEADV---- 110
+ + S N + + R V + ++ KYLG + +
Sbjct: 712 KWNVSINAGKCANVTFTNAIRDCPGVTINGSLLSHTHEYKYLGVILDRSLTFRRHITSLQ 771
Query: 111 -SHRIQAGWLKWRRDSDVLCDKKVPLKLKGKFYRPAFRPALMYGRECWAVKSQ-HENQVS 168
S R + + W + K+ L K K Y+ P L Y + + + ++ H N++
Sbjct: 772 HSFRTRITKMNWL----LAARNKLSLDNKVKIYKCIVAPGLFYAIQVYGIAARTHLNKIR 827
Query: 169 VAEMRMLRWMSSKTRHDRTRNDTIRKRVEVAPIVEKLVENRLRWFGHVERRPVDAVVRRV 228
V + +MLR +S + RTR+ I ++V I +K+ E ++ ++ P R
Sbjct: 828 VLQAKMLRKISGAPWYMRTRD--IECDLKVPKIGDKIREVAKKYHERLDSHPNSLARRLG 885
Query: 229 YQMEDSQVKRGRGRPR 244
+ S R R R R
Sbjct: 886 VAAQRSASPRSRVRRR 901
>PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 1025
Score = 36.6 bits (83), Expect = 0.067
Identities = 37/148 (25%), Positives = 60/148 (40%), Gaps = 30/148 (20%)
Query: 23 MLFADDVVLVGESREEVNGRLETWRQALEAYGFRLSRSKTEYIECNFSGRRSRSTLEVKV 82
++FADD+VL+ E+RE + L L+ G + K + SG+ + +KV
Sbjct: 551 LVFADDLVLLAETREGLQASLSRIEAGLQEQGLEMMPRKCHTLALVPSGKEKK----IKV 606
Query: 83 EDH-----------IIPQVTRSKYLGSFVQNDGEIEADVS-----HRIQAGWLKWRRDSD 126
E H + + KYLG + G I+ ++ R+ A LK ++
Sbjct: 607 ETHKPFTVGNQEITQLGHADQWKYLGVVYNSYGPIQVKINIAGDLQRVTAAPLKPQQRMA 666
Query: 127 VLCDKKVPLKLKGKFYRPAFRPALMYGR 154
+L G F P F L+ GR
Sbjct: 667 IL----------GMFLIPRFIHKLVLGR 684
>RUV2_XENLA (Q9DE27) RuvB-like 2 (EC 3.6.1.-) (Reptin)
Length = 462
Score = 32.0 bits (71), Expect = 1.6
Identities = 25/103 (24%), Positives = 49/103 (47%), Gaps = 9/103 (8%)
Query: 26 ADDVVLVGESREEVN--GRLETWRQALEAYGFRLSRSKTEYIECNFSGRRSRSTLEVKVE 83
A DV+ + ++ ++ GR T + +A G S+T++++C + R + V
Sbjct: 188 AGDVITIDKATGKITKLGRAFTRARDYDAMG-----SQTKFVQCPDGELQKRKEVVHTVS 242
Query: 84 DHIIPQVTRSK--YLGSFVQNDGEIEADVSHRIQAGWLKWRRD 124
H I + +L F + GEI+++V +I A +WR +
Sbjct: 243 LHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREE 285
>RUV2_MOUSE (Q9WTM5) RuvB-like 2 (EC 3.6.1.-) (p47 protein)
Length = 463
Score = 32.0 bits (71), Expect = 1.6
Identities = 25/103 (24%), Positives = 50/103 (48%), Gaps = 9/103 (8%)
Query: 26 ADDVVLVGESREEVN--GRLETWRQALEAYGFRLSRSKTEYIECNFSGRRSRSTLEVKVE 83
A DV+ + ++ +++ GR T + +A G S+T++++C + R + V
Sbjct: 189 AGDVITIDKATGKISKLGRSFTRARDYDAMG-----SQTKFVQCPDGELQKRKEVVHTVS 243
Query: 84 DHIIPQVTRSK--YLGSFVQNDGEIEADVSHRIQAGWLKWRRD 124
H I + +L F + GEI+++V +I A +WR +
Sbjct: 244 LHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREE 286
>RUV2_HUMAN (Q9Y230) RuvB-like 2 (EC 3.6.1.-) (48-kDa TATA
box-binding protein-interacting protein) (48-kDa
TBP-interacting protein) (TIP49b) (Repressing pontin 52)
(Reptin 52) (51 kDa erythrocyte cytosolic protein)
(ECP-51) (TIP60-associated protein 54-b
Length = 463
Score = 32.0 bits (71), Expect = 1.6
Identities = 25/103 (24%), Positives = 50/103 (48%), Gaps = 9/103 (8%)
Query: 26 ADDVVLVGESREEVN--GRLETWRQALEAYGFRLSRSKTEYIECNFSGRRSRSTLEVKVE 83
A DV+ + ++ +++ GR T + +A G S+T++++C + R + V
Sbjct: 189 AGDVITIDKATGKISKLGRSFTRARDYDAMG-----SQTKFVQCPDGELQKRKEVVHTVS 243
Query: 84 DHIIPQVTRSK--YLGSFVQNDGEIEADVSHRIQAGWLKWRRD 124
H I + +L F + GEI+++V +I A +WR +
Sbjct: 244 LHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREE 286
>YMJ3_YEAST (Q04500) Hypothetical 103.0 kDa protein in RAD10-PRS4
intergenic region
Length = 899
Score = 31.6 bits (70), Expect = 2.1
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 10/72 (13%)
Query: 136 KLKGKFYRPAFRPALMYGRECWAVKSQHENQ---VSVAEMRML-------RWMSSKTRHD 185
K+K K YR + LM RE AV S +N+ ++ A+ RM +W +H
Sbjct: 399 KIKSKTYRKIKKKELMKNRELAAVSSDEDNEDHDIARAKERMTLKHKTNSKWAKDMIKHG 458
Query: 186 RTRNDTIRKRVE 197
T + R+ +E
Sbjct: 459 MTNDAETREEME 470
>RUV2_BRARE (P83571) RuvB-like 2 (EC 3.6.1.-) (Reptin) (zReptin)
Length = 463
Score = 30.8 bits (68), Expect = 3.7
Identities = 24/103 (23%), Positives = 50/103 (48%), Gaps = 9/103 (8%)
Query: 26 ADDVVLVGESREEVN--GRLETWRQALEAYGFRLSRSKTEYIECNFSGRRSRSTLEVKVE 83
A DV+ + ++ +++ GR T + +A G ++T++++C + R + V
Sbjct: 189 AGDVITIDKATGKISKLGRSFTRARDYDAMG-----AQTQFVQCPEGELQKRKEVVHTVS 243
Query: 84 DHIIPQVTRSK--YLGSFVQNDGEIEADVSHRIQAGWLKWRRD 124
H I + +L F + GEI+++V +I A +WR +
Sbjct: 244 LHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVSEWREE 286
>PCF1_MOUSE (P59114) Phosphorylated CTD interacting factor 1
Length = 706
Score = 30.4 bits (67), Expect = 4.8
Identities = 15/47 (31%), Positives = 28/47 (58%), Gaps = 1/47 (2%)
Query: 221 VDAVVRRVYQMEDSQVKRGRGRPRKTIRET-IRKDLEVNELDPNLVY 266
V+ + ++Y + VKR R + ++E I +++E +EL+P LVY
Sbjct: 356 VEGICSKIYHISLEYVKRIREKHLAVLKENNIPEEVEASELEPRLVY 402
>YCF2_MESVI (Q9MUP8) Protein ycf2 (RF2)
Length = 890
Score = 30.0 bits (66), Expect = 6.2
Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Query: 13 EHIQELAPRCMLFADDVVLVGESREEVNGRLE 44
+ IQ ++P C+L+ D++ + E REE N +LE
Sbjct: 431 KQIQNISP-CILYIDEIDSIAERREEANKQLE 461
>TAF1_DROME (P51123) Transcription initiation factor TFIID subunit 1
(EC 2.7.1.37) (Transcription initiation factor TFIID 230
kDa subunit) (TAFII-230) (TAFII250) (TBP-associated
factor 230 kDa) (p230)
Length = 2065
Score = 30.0 bits (66), Expect = 6.2
Identities = 16/37 (43%), Positives = 23/37 (61%), Gaps = 1/37 (2%)
Query: 225 VRRVYQMEDSQVKRGRGRPRKTIRETIRKDLEVNELD 261
VR+ +VKRGRGRPRK R+ + +DL+ + D
Sbjct: 1778 VRKPAPPGPGEVKRGRGRPRKQ-RDPVEEDLQCSTDD 1813
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.136 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,666,386
Number of Sequences: 164201
Number of extensions: 1224419
Number of successful extensions: 3027
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 3020
Number of HSP's gapped (non-prelim): 12
length of query: 270
length of database: 59,974,054
effective HSP length: 108
effective length of query: 162
effective length of database: 42,240,346
effective search space: 6842936052
effective search space used: 6842936052
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)
Medicago: description of AC127169.2