BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.8 + phase: 0 /pseudo
(250 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CCI1_ARATH (Q9M643) Cycloeucalenol cycloisomerase (EC 5.5.1.9) (... 267 2e-71
NU1M_APILI (P34847) NADH-ubiquinone oxidoreductase chain 1 (EC 1... 38 0.020
YO67_BACAN (Q81QF8) Hypothetical UPF0078 protein BA2467/GBAA2467... 36 0.100
YPRF_ZYMMO (Q9RH13) Hypothetical UPF0060 protein in purF 5'region 32 1.4
YP00_BACC1 (Q737Z5) Hypothetical UPF0078 protein BCE2500 32 1.4
APPC_ECOLI (P26459) Cytochrome bd-II oxidase subunit I (EC 1.10.... 31 2.5
YN98_BACCR (Q81DG8) Hypothetical UPF0078 protein BC2398 31 3.2
BXEN_CLOBO (P46082) Botulinum neurotoxin type E, nontoxic component 31 3.2
CYDA_AZOVI (Q09049) Cytochrome d ubiquinol oxidase subunit I (EC... 30 4.2
BXEN_CLOBU (Q06366) Botulinum neurotoxin type E, nontoxic component 30 4.2
S361_HUMAN (Q7Z2H8) Proton-coupled amino acid transporter 1 (Pro... 30 5.5
RSMB_XYLFA (Q9PEV0) Ribosomal RNA small subunit methyltransferas... 30 7.1
MBI3_YEAST (Q9ZZW7) Cytochrome b mRNA maturase bI3 30 7.1
SAT7_RAT (Q9R0A3) Spermatogenesis associated protein 7 homolog (... 29 9.3
>CCI1_ARATH (Q9M643) Cycloeucalenol cycloisomerase (EC 5.5.1.9)
(Cycloeucalenol--obtusifoliol isomerase) (Cyclopropyl
sterol isomerase)
Length = 280
Score = 267 bits (683), Expect = 2e-71
Identities = 150/283 (53%), Positives = 175/283 (61%), Gaps = 66/283 (23%)
Query: 24 SASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISA 83
S S +PSLW APN SKRWGELFFL YTPFWL+L LGIVVPYKLYE FTELEYLLL L+SA
Sbjct: 2 SGSSSPSLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLYETFTELEYLLLALVSA 61
Query: 84 VPAFLIPMLFVGKADKGISWQDRYWVKSMDNNLQLC---------WELFLDSLL------ 128
VPAF+IPML VGKAD+ + W+DRYWVK+ NL + W + +L
Sbjct: 62 VPAFVIPMLLVGKADRSLCWKDRYWVKA---NLWIIVFSYVGNYFWTHYFFKVLGASYTF 118
Query: 129 -------------FHSSGCILYLSFMEN---------------------EQSYLETLAIS 154
F + C L+ N E +++ LA+S
Sbjct: 119 PSWKMNNVPHTTFFLTHVCFLFYHVASNITLRRLRHSTADLPDSLKWCFEAAWI--LALS 176
Query: 155 NFPYY------------EFVDRESMYKVGSLFYAIYFIVSFPMFLRIDEKPGDKWDLPRV 202
F Y EFVDR +MY+VG LFYAIYFIVSFPMF R+DEK D+WDL RV
Sbjct: 177 YFIAYLETIAIANFPYYEFVDRSAMYRVGCLFYAIYFIVSFPMFFRMDEKSTDEWDLSRV 236
Query: 203 AVDALGAAMLVTIILDLWRIFLGPIVPVSEAKQCSPTGLPWFS 245
AVDALGAAMLVTIILDLWR+FLGPIVP+ E + C +GLPWFS
Sbjct: 237 AVDALGAAMLVTIILDLWRLFLGPIVPLPEGQNCLQSGLPWFS 279
>NU1M_APILI (P34847) NADH-ubiquinone oxidoreductase chain 1 (EC
1.6.5.3)
Length = 305
Score = 38.1 bits (87), Expect = 0.020
Identities = 38/167 (22%), Positives = 69/167 (40%), Gaps = 33/167 (19%)
Query: 37 LSKRWGELFF-----LLYTP---FWLSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFL 88
LSK W FF +Y+P F+LSL + I+ P+ + + E L + L+ + +
Sbjct: 57 LSKEW--FFFNYSNLFIYSPMLMFFLSLVMWILYPWFGFMYYIEFSILFMLLVLGLSVY- 113
Query: 89 IPMLFVGKADKGISWQDRYWVKSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQSYL 148
P+LFVG W+ + + + L + F + L S M
Sbjct: 114 -PVLFVG------------WISNCNYAILGSMRLVSTMISFEINLFFLVFSLM------- 153
Query: 149 ETLAISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPMFLRIDEKPGD 195
+ + +F + EF ++ K L Y +Y ++ M + ++ P D
Sbjct: 154 --MMVESFSFNEFFFFQNNIKFAILLYPLYLMMFTSMLIELNRTPFD 198
>YO67_BACAN (Q81QF8) Hypothetical UPF0078 protein
BA2467/GBAA2467/BAS2295
Length = 189
Score = 35.8 bits (81), Expect = 0.100
Identities = 29/109 (26%), Positives = 55/109 (49%), Gaps = 15/109 (13%)
Query: 1 MVSSILFSKSLFCVSV------LEISSSSSASPTPSLWFAPNLSKRWGELFFLL-YTPFW 53
+V+S LF L V ++I S +P A N+ + +G+ +F+ +
Sbjct: 17 LVASYLFGNILTAYIVTKWRHNVDIRDEGSGNPG-----ARNMGRVYGKGYFVATFLGDA 71
Query: 54 LSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPMLFVGKADKGIS 102
+ ++ + + L+E+FT +++L L++ + + PMLF GK KGIS
Sbjct: 72 IKGAIVVSIAKYLFEDFT---FVMLTLLAVIMGHIYPMLFKGKGGKGIS 117
>YPRF_ZYMMO (Q9RH13) Hypothetical UPF0060 protein in purF 5'region
Length = 107
Score = 32.0 bits (71), Expect = 1.4
Identities = 23/97 (23%), Positives = 48/97 (48%), Gaps = 5/97 (5%)
Query: 122 LFLDSLLFHSSGCILYLSFMENEQSYLETL-AISNFPYYE----FVDRESMYKVGSLFYA 176
L++ + L +GC + +++ +S L L I++ + F E+ K +++
Sbjct: 5 LYIPAALAEITGCFSFWAWIRLHKSPLWLLPGIASLLLFAWLLTFSPAENAGKAYAVYGG 64
Query: 177 IYFIVSFPMFLRIDEKPGDKWDLPRVAVDALGAAMLV 213
IY I+S +++ P + WDL A +GAA+++
Sbjct: 65 IYIIMSLLWSWKVEATPPEHWDLIGAAFCLVGAAIIL 101
>YP00_BACC1 (Q737Z5) Hypothetical UPF0078 protein BCE2500
Length = 182
Score = 32.0 bits (71), Expect = 1.4
Identities = 29/108 (26%), Positives = 54/108 (49%), Gaps = 13/108 (12%)
Query: 1 MVSSILFSKSLFCVSV------LEISSSSSASPTPSLWFAPNLSKRWGELFFLLYTPFWL 54
+V+S LF L V ++I + S +P A N+ + +G+ +F++ T
Sbjct: 10 LVASYLFGNILTAYIVTKWRHDVDIRNEGSGNPG-----ARNMGRVYGKGYFIV-TFLGD 63
Query: 55 SLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPMLFVGKADKGIS 102
++ IVV Y F + +++L L++ + + P++F GK KGIS
Sbjct: 64 AIKGAIVVSIAEYL-FGDSTFVMLALLAVLLGHIYPIVFKGKGGKGIS 110
>APPC_ECOLI (P26459) Cytochrome bd-II oxidase subunit I (EC
1.10.3.-)
Length = 514
Score = 31.2 bits (69), Expect = 2.5
Identities = 30/149 (20%), Positives = 60/149 (40%), Gaps = 10/149 (6%)
Query: 36 NLSKRWGELF---FLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPML 92
++++ WG+LF F L L++ + Y N+ + + A+ AF +
Sbjct: 50 DMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALMAFFLEST 109
Query: 93 FVGKADKGISWQDRY------WVKSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQS 146
FVG G ++Y W+ + +NL W L + + + +G + + E +
Sbjct: 110 FVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFDIDTLRMEMT 169
Query: 147 YLETLAISNFPYYEFVDR-ESMYKVGSLF 174
L + +FV + Y G++F
Sbjct: 170 SFSELVFNPVSQVKFVHTVMAGYVTGAMF 198
>YN98_BACCR (Q81DG8) Hypothetical UPF0078 protein BC2398
Length = 182
Score = 30.8 bits (68), Expect = 3.2
Identities = 21/69 (30%), Positives = 38/69 (54%), Gaps = 2/69 (2%)
Query: 34 APNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPMLF 93
A N+ + +G+ +F+ T ++ IVV Y F + +++L L++ + + P+LF
Sbjct: 44 ARNMGRVYGKGYFIA-TFLGDAIKGAIVVAVAKYL-FEDPTFVMLTLLAVIIGHIYPVLF 101
Query: 94 VGKADKGIS 102
GK KGIS
Sbjct: 102 KGKGGKGIS 110
>BXEN_CLOBO (P46082) Botulinum neurotoxin type E, nontoxic component
Length = 1162
Score = 30.8 bits (68), Expect = 3.2
Identities = 23/110 (20%), Positives = 44/110 (39%), Gaps = 25/110 (22%)
Query: 31 LWFAPNLSKRWGELFF------------LLYTPFWLSLSLGIVVPYKLYENFTELEYLLL 78
+WF P L+ ++ E + LY + + + + +PY+L F LEY L
Sbjct: 167 IWFQPFLTHKYDEFYVDPALELIKCLIKSLYYLYGIKPNDNLNIPYRLRNEFNSLEYSEL 226
Query: 79 GLISAV-------------PAFLIPMLFVGKADKGISWQDRYWVKSMDNN 115
+I + P + I F+ + +++ Y +K +NN
Sbjct: 227 NMIDFLISGGIDYKLLNTNPYWFIDKYFIDTSKNFEKYKNDYEIKIKNNN 276
>CYDA_AZOVI (Q09049) Cytochrome d ubiquinol oxidase subunit I (EC
1.10.3.-)
Length = 537
Score = 30.4 bits (67), Expect = 4.2
Identities = 32/152 (21%), Positives = 60/152 (39%), Gaps = 16/152 (10%)
Query: 36 NLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISA------VPAFLI 89
++ K WG+LF + F L ++ GI + ++ N+ + + + A + AF +
Sbjct: 52 DMVKFWGKLFGI---NFALGVTTGITMEFQFGTNWAYYSHYVGDIFGAPLAIEGLTAFFL 108
Query: 90 PMLFVGKADKGISWQDRY------WVKSMDNNLQLCWELFLDSLLFHSSGCILYLSFMEN 143
F+G G + W+ ++ +NL W L + + H G M
Sbjct: 109 ESTFIGMFFFGWDRLSKIQHLAVTWLVALGSNLSALWILVANGWMQHPVGAEFNFETMRM 168
Query: 144 EQSYLETLAISNFPYYEFVDR-ESMYKVGSLF 174
E L ++ +FV S Y G++F
Sbjct: 169 ELVDFGALLLNPVAQVKFVHTVASGYVTGAVF 200
>BXEN_CLOBU (Q06366) Botulinum neurotoxin type E, nontoxic component
Length = 1162
Score = 30.4 bits (67), Expect = 4.2
Identities = 24/110 (21%), Positives = 46/110 (41%), Gaps = 25/110 (22%)
Query: 31 LWFAPNLSKRWGELFF------------LLYTPFWLSLSLGIVVPYKLYENFTELEY--- 75
+WF P L+ ++ E + LY + + + + +PY+L F LEY
Sbjct: 167 IWFQPFLTHKYDEFYVDPALELIKCLIKSLYYLYGIKPNDNLNIPYRLRNEFNSLEYSEL 226
Query: 76 -----LLLG-----LISAVPAFLIPMLFVGKADKGISWQDRYWVKSMDNN 115
L+ G L++ P + I F+ + +++ Y +K +NN
Sbjct: 227 DMIDFLISGGIDYKLLNTNPYWFIDKYFIDTSKNFEKYKNDYEIKIKNNN 276
>S361_HUMAN (Q7Z2H8) Proton-coupled amino acid transporter 1
(Proton/amino acid transporter 1) (Solute carrier family
36 member 1)
Length = 476
Score = 30.0 bits (66), Expect = 5.5
Identities = 21/57 (36%), Positives = 32/57 (55%), Gaps = 4/57 (7%)
Query: 126 SLLFHSSGCILYLSFMENEQSYLETLAISNFPYYEFVDRESMYKVGSLF-YAIYFIV 181
++L+ S GC+ YL F N Q + TL + N Y+ V + +Y +G F YA+ F V
Sbjct: 300 TILYISLGCLGYLQFGANIQGSI-TLNLPNCWLYQSV--KLLYSIGIFFTYALQFYV 353
>RSMB_XYLFA (Q9PEV0) Ribosomal RNA small subunit methyltransferase B
(EC 2.1.1.-) (rRNA (cytosine-C(5)-)-methyltransferase)
(16S rRNA m5C967 methyltransferase)
Length = 421
Score = 29.6 bits (65), Expect = 7.1
Identities = 22/80 (27%), Positives = 36/80 (44%), Gaps = 15/80 (18%)
Query: 17 LEISSSSSASPTPSLWFAPNLSKRWGELFFLLY------TPFWLSLSLGIVVPYKLYENF 70
L + SS +A P W A + WGEL ++ P WL ++L + +
Sbjct: 123 LPVVSSDAAWPR---WLAERIRADWGELAEAIFDASLKPAPMWLRVNL------RYGDRH 173
Query: 71 TELEYLLLGLISAVPAFLIP 90
T ++ L + AVP+ L+P
Sbjct: 174 TYVQRLQAAGLEAVPSGLVP 193
>MBI3_YEAST (Q9ZZW7) Cytochrome b mRNA maturase bI3
Length = 517
Score = 29.6 bits (65), Expect = 7.1
Identities = 12/50 (24%), Positives = 27/50 (54%)
Query: 100 GISWQDRYWVKSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQSYLE 149
G + +D Y+ M N LCW +F+ + ++ I+Y + + N+ + ++
Sbjct: 168 GFNMEDPYYSNMMLNKSVLCWNIFIWMMNYYIIQLIIYNNMIWNKNNMVK 217
>SAT7_RAT (Q9R0A3) Spermatogenesis associated protein 7 homolog (Rat
sperm DNA no.3) (RSD-3) (Fertility related protein WMP1)
Length = 526
Score = 29.3 bits (64), Expect = 9.3
Identities = 19/53 (35%), Positives = 25/53 (46%), Gaps = 6/53 (11%)
Query: 97 ADKGISWQDRYWVKSMDNNLQL------CWELFLDSLLFHSSGCILYLSFMEN 143
A + Q+ W KS D LQ C + DS ++ +LYLSFMEN
Sbjct: 255 APLSLQGQNLAWDKSKDGILQPSSERAPCTQFSPDSKIYSDEEELLYLSFMEN 307
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.141 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,835,271
Number of Sequences: 164201
Number of extensions: 1247486
Number of successful extensions: 3563
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 3558
Number of HSP's gapped (non-prelim): 15
length of query: 250
length of database: 59,974,054
effective HSP length: 108
effective length of query: 142
effective length of database: 42,240,346
effective search space: 5998129132
effective search space used: 5998129132
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC126782.8