BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126781.9 + phase: 0
(195 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 137 2e-32
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 135 5e-32
M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810... 95 9e-20
M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240... 67 3e-11
YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein 50 3e-06
YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B) 44 2e-04
YMD9_YEAST (Q03434) Transposon Ty1 protein B 44 3e-04
YJZ7_YEAST (P47098) Transposon Ty1 protein B 44 3e-04
YME4_YEAST (Q04711) Transposon Ty1 protein B 37 0.029
YMU0_YEAST (Q04670) Transposon Ty1 protein B 36 0.065
YMT5_YEAST (Q04214) Transposon Ty1 protein B 35 0.11
YJZ9_YEAST (P47100) Transposon Ty1 protein B 35 0.11
SKM1_YEAST (Q12469) Serine/threonine-protein kinase SKM1 (EC 2.7... 35 0.11
YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein 32 0.94
CLA4_ASHGO (Q9HFW2) Serine/threonine-protein kinase CLA4 (EC 2.7... 32 1.2
CLA4_YEAST (P48562) Serine/threonine-protein kinase CLA4 (EC 2.7... 30 2.7
CLP1_CHLPN (Q9Z832) ATP-dependent Clp protease proteolytic subun... 29 6.1
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 137 bits (345), Expect = 2e-32
Identities = 72/190 (37%), Positives = 112/190 (58%), Gaps = 2/190 (1%)
Query: 1 MNQPRTEHWEAALRVVRFLKGNPGQGIFFGSKSDLRLHGWCDSDWASCPLTRRSVTGWIV 60
+ P EHWEA ++R+L+G G + FG SD L G+ D+D A R+S TG++
Sbjct: 1140 LENPGKEHWEAVKWILRYLRGTTGDCLCFGG-SDPILKGYTDADMAGDIDNRKSSTGYLF 1198
Query: 61 QLGDSPISWKTKKQHTVSRSSAEAEYRSMANTTCELKWLKGILSNLGVVHDKPMIIHCDS 120
ISW++K Q V+ S+ EAEY + T E+ WLK L LG+ H K +++CDS
Sbjct: 1199 TFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGL-HQKEYVVYCDS 1257
Query: 121 QAAIHIAKNPVFHERTKHIEVDCHFVRNEVLKNNILPVYVSTANQPADIFTKALGKPQFE 180
Q+AI ++KN ++H RTKHI+V H++R V ++ + +ST PAD+ TK + + +FE
Sbjct: 1258 QSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVPRNKFE 1317
Query: 181 FLLGKLGIRN 190
+G+ +
Sbjct: 1318 LCKELVGMHS 1327
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 135 bits (341), Expect = 5e-32
Identities = 73/186 (39%), Positives = 111/186 (59%), Gaps = 3/186 (1%)
Query: 6 TEHWEAALRVVRFLKGNPGQGIFFGSKS--DLRLHGWCDSDWASCPLTRRSVTGWIVQLG 63
+E W+ RV+R+LKG + F + ++ G+ DSDWA + R+S TG++ ++
Sbjct: 1215 SELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTGYLFKMF 1274
Query: 64 D-SPISWKTKKQHTVSRSSAEAEYRSMANTTCELKWLKGILSNLGVVHDKPMIIHCDSQA 122
D + I W TK+Q++V+ SS EAEY ++ E WLK +L+++ + + P+ I+ D+Q
Sbjct: 1275 DFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLENPIKIYEDNQG 1334
Query: 123 AIHIAKNPVFHERTKHIEVDCHFVRNEVLKNNILPVYVSTANQPADIFTKALGKPQFEFL 182
I IA NP H+R KHI++ HF R +V N I Y+ T NQ ADIFTK L +F L
Sbjct: 1335 CISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKPLPAARFVEL 1394
Query: 183 LGKLGI 188
KLG+
Sbjct: 1395 RDKLGL 1400
>M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810
(ORF240b)
Length = 240
Score = 95.1 bits (235), Expect = 9e-20
Identities = 45/98 (45%), Positives = 61/98 (61%)
Query: 1 MNQPRTEHWEAALRVVRFLKGNPGQGIFFGSKSDLRLHGWCDSDWASCPLTRRSVTGWIV 60
M++P ++ RV+R++KG G++ S L + +CDSDWA C TRRS TG+
Sbjct: 128 MHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCT 187
Query: 61 QLGDSPISWKTKKQHTVSRSSAEAEYRSMANTTCELKW 98
LG + ISW K+Q TVSRSS E EYR++A T EL W
Sbjct: 188 FLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225
>M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240
(ORF111a)
Length = 111
Score = 67.0 bits (162), Expect = 3e-11
Identities = 29/54 (53%), Positives = 40/54 (73%)
Query: 5 RTEHWEAALRVVRFLKGNPGQGIFFGSKSDLRLHGWCDSDWASCPLTRRSVTGW 58
RT +A +V+ ++KG GQG+F+ + SDL+L + DSDWASCP TRRSVTG+
Sbjct: 25 RTAQMQAVYKVLHYVKGTVGQGLFYSATSDLQLKAFADSDWASCPDTRRSVTGF 78
>YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein
Length = 308
Score = 50.4 bits (119), Expect = 3e-06
Identities = 28/97 (28%), Positives = 50/97 (50%), Gaps = 1/97 (1%)
Query: 1 MNQPRTEHWEAALRVVRFLKGNPGQGIFFGSKSDLRLHGWCDSDWASCPLTRRSVTGWIV 60
+ +PR H E+A RV+R+L + + S S + L +CD+ + S G++
Sbjct: 212 LREPRAIHLESARRVLRYLYTTRSMCLKYRSGSQVALTVYCDASHGAIHDLPHSTGGYVT 271
Query: 61 QLGDSPISWKTKK-QHTVSRSSAEAEYRSMANTTCEL 96
L +P++W +KK + + S EAEY + + T E+
Sbjct: 272 LLAGAPVTWSSKKLKGVIPVPSTEAEYITASETVMEI 308
>YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B)
Length = 1770
Score = 44.3 bits (103), Expect = 2e-04
Identities = 42/158 (26%), Positives = 70/158 (43%), Gaps = 7/158 (4%)
Query: 31 SKSDLRLHGWCDSDWASCPLTRRSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMA 90
+K D +L D+ + + P + + G I L I K+ K S+ EAE +++
Sbjct: 1614 TKPDNKLVAISDASYGNQPYYKSQI-GNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVS 1672
Query: 91 NTTCELKWLKGILSNLGVVHDKPMI--IHCDSQAAIHIAKNPVFHE-RTKHIEVDCHFVR 147
L L ++ L + KP+I + DS++ I I K+ + R + +R
Sbjct: 1673 EAIPLLNNLSHLVQEL---NKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLR 1729
Query: 148 NEVLKNNILPVYVSTANQPADIFTKALGKPQFEFLLGK 185
+EV NN+ Y+ T AD+ TK L F+ L K
Sbjct: 1730 DEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNK 1767
>YMD9_YEAST (Q03434) Transposon Ty1 protein B
Length = 1328
Score = 43.5 bits (101), Expect = 3e-04
Identities = 41/158 (25%), Positives = 71/158 (43%), Gaps = 7/158 (4%)
Query: 31 SKSDLRLHGWCDSDWASCPLTRRSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMA 90
++ D +L D+ + + P + + G I L I K+ K S+ EAE +++
Sbjct: 1172 TEPDNKLVAISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAIS 1230
Query: 91 NTTCELKWLKGILSNLGVVHDKPMI--IHCDSQAAIHIAKNPVFHE-RTKHIEVDCHFVR 147
+ L L ++ L + KP+I + DS++ I I K+ + R + +R
Sbjct: 1231 ESVPLLNNLSYLIQEL---NKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLR 1287
Query: 148 NEVLKNNILPVYVSTANQPADIFTKALGKPQFEFLLGK 185
+EV NN+ Y+ T AD+ TK L F+ L K
Sbjct: 1288 DEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNK 1325
>YJZ7_YEAST (P47098) Transposon Ty1 protein B
Length = 1755
Score = 43.5 bits (101), Expect = 3e-04
Identities = 41/158 (25%), Positives = 71/158 (43%), Gaps = 7/158 (4%)
Query: 31 SKSDLRLHGWCDSDWASCPLTRRSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMA 90
++ D +L D+ + + P + + G I L I K+ K S+ EAE +++
Sbjct: 1599 TEPDNKLVAISDASYGNQPYYKSQI-GNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAIS 1657
Query: 91 NTTCELKWLKGILSNLGVVHDKPMI--IHCDSQAAIHIAKNPVFHE-RTKHIEVDCHFVR 147
+ L L ++ L + KP+I + DS++ I I K+ + R + +R
Sbjct: 1658 ESVPLLNNLSYLIQEL---NKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLR 1714
Query: 148 NEVLKNNILPVYVSTANQPADIFTKALGKPQFEFLLGK 185
+EV NN+ Y+ T AD+ TK L F+ L K
Sbjct: 1715 DEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNK 1752
>YME4_YEAST (Q04711) Transposon Ty1 protein B
Length = 1328
Score = 37.0 bits (84), Expect = 0.029
Identities = 39/158 (24%), Positives = 68/158 (42%), Gaps = 7/158 (4%)
Query: 31 SKSDLRLHGWCDSDWASCPLTRRSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMA 90
++ D +L D+ + + P + + G I L I K+ K S+ EAE +++
Sbjct: 1172 TEPDNKLVAISDASYGNQPYYKSQI-GNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAIS 1230
Query: 91 NTTCELKWLKGILSNLGVVHDKPMI--IHCDSQAAIHIA-KNPVFHERTKHIEVDCHFVR 147
+ L L ++ L + KP+ + DS++ I I N R + +R
Sbjct: 1231 ESVPLLNNLSHLVQEL---NKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLR 1287
Query: 148 NEVLKNNILPVYVSTANQPADIFTKALGKPQFEFLLGK 185
+EV N++ Y+ T AD+ TK L F+ L K
Sbjct: 1288 DEVSGNHLHVCYIETKKNIADVMTKPLPIKTFKLLTNK 1325
>YMU0_YEAST (Q04670) Transposon Ty1 protein B
Length = 1328
Score = 35.8 bits (81), Expect = 0.065
Identities = 36/136 (26%), Positives = 58/136 (42%), Gaps = 6/136 (4%)
Query: 53 RSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMANTTCELKWLKGILSNLGVVHDK 112
+S G I L I K+ K S+ EAE +++ + L L ++ L + K
Sbjct: 1193 KSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSHLVQEL---NKK 1249
Query: 113 PMI--IHCDSQAAIHIA-KNPVFHERTKHIEVDCHFVRNEVLKNNILPVYVSTANQPADI 169
P+ + DS++ I I N R + +R+EV N++ Y+ T AD+
Sbjct: 1250 PITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADV 1309
Query: 170 FTKALGKPQFEFLLGK 185
TK L F+ L K
Sbjct: 1310 MTKPLPIKTFKLLTNK 1325
>YMT5_YEAST (Q04214) Transposon Ty1 protein B
Length = 1328
Score = 35.0 bits (79), Expect = 0.11
Identities = 36/136 (26%), Positives = 57/136 (41%), Gaps = 6/136 (4%)
Query: 53 RSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMANTTCELKWLKGILSNLGVVHDK 112
+S G I L I K+ K S+ EAE +++ + L L ++ L K
Sbjct: 1193 KSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSYLIQELD---KK 1249
Query: 113 PMI--IHCDSQAAIHIA-KNPVFHERTKHIEVDCHFVRNEVLKNNILPVYVSTANQPADI 169
P+ + DS++ I I N R + +R+EV N++ Y+ T AD+
Sbjct: 1250 PITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADV 1309
Query: 170 FTKALGKPQFEFLLGK 185
TK L F+ L K
Sbjct: 1310 MTKPLPIKTFKLLTNK 1325
>YJZ9_YEAST (P47100) Transposon Ty1 protein B
Length = 1755
Score = 35.0 bits (79), Expect = 0.11
Identities = 36/136 (26%), Positives = 57/136 (41%), Gaps = 6/136 (4%)
Query: 53 RSVTGWIVQLGDSPISWKTKKQHTVSRSSAEAEYRSMANTTCELKWLKGILSNLGVVHDK 112
+S G I L I K+ K S+ EAE +++ + L L ++ L K
Sbjct: 1620 KSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSYLIQELD---KK 1676
Query: 113 PMI--IHCDSQAAIHIA-KNPVFHERTKHIEVDCHFVRNEVLKNNILPVYVSTANQPADI 169
P+ + DS++ I I N R + +R+EV N++ Y+ T AD+
Sbjct: 1677 PITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADV 1736
Query: 170 FTKALGKPQFEFLLGK 185
TK L F+ L K
Sbjct: 1737 MTKPLPIKTFKLLTNK 1752
>SKM1_YEAST (Q12469) Serine/threonine-protein kinase SKM1 (EC
2.7.1.37) (Protein kinase 75490 D)
Length = 655
Score = 35.0 bits (79), Expect = 0.11
Identities = 13/33 (39%), Positives = 21/33 (63%)
Query: 18 FLKGNPGQGIFFGSKSDLRLHGWCDSDWASCPL 50
F + N + IF ++++ LHGW D+ +A CPL
Sbjct: 87 FYESNSKRSIFISTRTERDLHGWLDAIFAKCPL 119
>YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein
Length = 1803
Score = 32.0 bits (71), Expect = 0.94
Identities = 21/101 (20%), Positives = 44/101 (42%)
Query: 80 SSAEAEYRSMANTTCELKWLKGILSNLGVVHDKPMIIHCDSQAAIHIAKNPVFHERTKHI 139
SS EAE ++ + + LK L LG + +++ DS+ AI + K
Sbjct: 1682 SSTEAELHAIYEGYADSETLKVTLKELGEGDNNDIVMITDSKPAIQGLNRSYQQPKEKFT 1741
Query: 140 EVDCHFVRNEVLKNNILPVYVSTANQPADIFTKALGKPQFE 180
+ ++ ++ + +I + ++ AD+ TK + F+
Sbjct: 1742 WIKTEIIKEKIKEKSIKLLKITGKGNIADLLTKPVSASDFK 1782
>CLA4_ASHGO (Q9HFW2) Serine/threonine-protein kinase CLA4 (EC
2.7.1.37)
Length = 793
Score = 31.6 bits (70), Expect = 1.2
Identities = 11/26 (42%), Positives = 18/26 (68%)
Query: 25 QGIFFGSKSDLRLHGWCDSDWASCPL 50
+ +F +K++L LH W DS ++ CPL
Sbjct: 144 KSVFIATKTELDLHTWLDSIFSKCPL 169
>CLA4_YEAST (P48562) Serine/threonine-protein kinase CLA4 (EC
2.7.1.37)
Length = 842
Score = 30.4 bits (67), Expect = 2.7
Identities = 11/29 (37%), Positives = 18/29 (61%)
Query: 22 NPGQGIFFGSKSDLRLHGWCDSDWASCPL 50
N + I+ +K++ LH W D+ +A CPL
Sbjct: 152 NSKKSIYIATKTESDLHSWLDAIFAKCPL 180
>CLP1_CHLPN (Q9Z832) ATP-dependent Clp protease proteolytic subunit
1 (EC 3.4.21.92) (Endopeptidase Clp 1)
Length = 191
Score = 29.3 bits (64), Expect = 6.1
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
Query: 140 EVDCHFVRNEVLKNN--ILPVYVSTANQPADIFTKALGK 176
++D H E+LK I+ VYV NQP DI KA+ +
Sbjct: 130 DLDIH--AREILKTKARIIDVYVEATNQPRDIIEKAIDR 166
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.135 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,502,902
Number of Sequences: 164201
Number of extensions: 905490
Number of successful extensions: 1632
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1613
Number of HSP's gapped (non-prelim): 19
length of query: 195
length of database: 59,974,054
effective HSP length: 104
effective length of query: 91
effective length of database: 42,897,150
effective search space: 3903640650
effective search space used: 3903640650
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)
Medicago: description of AC126781.9