BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126780.8 - phase: 0 /pseudo
(172 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
IM23_SCHPO (Q9USM7) Mitochondrial import inner membrane transloc... 38 0.010
IM7A_RAT (O35092) Mitochondrial import inner membrane translocas... 36 0.038
IM7B_HUMAN (O60830) Mitochondrial import inner membrane transloc... 35 0.086
IM22_HUMAN (Q9Y584) Mitochondrial import inner membrane transloc... 35 0.086
IM7B_MOUSE (Q9Z0V7) Mitochondrial import inner membrane transloc... 34 0.15
IM7A_MOUSE (Q9Z0V8) Mitochondrial import inner membrane transloc... 34 0.19
IM7A_HUMAN (Q99595) Mitochondrial import inner membrane transloc... 34 0.19
IM22_MOUSE (Q9CQ85) Mitochondrial import inner membrane transloc... 33 0.43
IM23_HUMAN (O14925) Mitochondrial import inner membrane transloc... 30 2.1
IM23_BRARE (Q7T2P6) Mitochondrial import inner membrane transloc... 30 2.1
GLS2_ARATH (Q9T0P4) Ferredoxin-dependent glutamate synthase 2, c... 30 3.6
RBS1_HUMAN (P29558) RNA binding motif, single-stranded interacti... 29 4.7
IM23_MOUSE (Q9WTQ8) Mitochondrial import inner membrane transloc... 29 4.7
LIPB_TROWT (Q83G63) Lipoyltransferase (EC 2.3.1.-) (Lipoyl-[acyl... 29 6.1
LIPB_TROW8 (Q83I07) Lipoyltransferase (EC 2.3.1.-) (Lipoyl-[acyl... 29 6.1
IM23_RAT (O35093) Mitochondrial import inner membrane translocas... 29 6.1
IM17_YEAST (P39515) Mitochondrial import inner membrane transloc... 29 6.1
ARC1_LACPL (P59391) N-acetyl-gamma-glutamyl-phosphate reductase ... 29 6.1
IM17_SCHPO (P87130) Mitochondrial import inner membrane transloc... 28 8.0
GLS1_ARATH (Q9ZNZ7) Ferredoxin-dependent glutamate synthase 1, c... 28 8.0
>IM23_SCHPO (Q9USM7) Mitochondrial import inner membrane translocase
subunit tim23
Length = 210
Score = 38.1 bits (87), Expect = 0.010
Identities = 22/67 (32%), Positives = 35/67 (51%), Gaps = 6/67 (8%)
Query: 104 GLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTSHEQIAQCAITGAAIS 163
G+ A +Y+G+ + R H W+NS AGA+TGA T + AI+ + ++
Sbjct: 139 GVLALVYNGINSLIGYKRQKHGWENSVAAGALTGALYKST------RGLRAMAISSSLVA 192
Query: 164 TAANLLT 170
TAA + T
Sbjct: 193 TAAGIWT 199
>IM7A_RAT (O35092) Mitochondrial import inner membrane translocase
subunit Tim17 A (Inner membrane preprotein translocase
Tim17a)
Length = 171
Score = 36.2 bits (82), Expect = 0.038
Identities = 28/71 (39%), Positives = 36/71 (50%), Gaps = 8/71 (11%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTSHEQIAQCAITG--- 159
WG GL+S + GM + RG D NS +GA+TGA LA + N + A+ G
Sbjct: 69 WG---GLFSTIDCGMVQIRGKEDPWNSITSGALTGAILA--ARNGPVAMVGSAAMGGILL 123
Query: 160 AAISTAANLLT 170
A I A LLT
Sbjct: 124 ALIEGAGILLT 134
>IM7B_HUMAN (O60830) Mitochondrial import inner membrane translocase
subunit Tim17 B (JM3)
Length = 172
Score = 35.0 bits (79), Expect = 0.086
Identities = 19/42 (45%), Positives = 24/42 (56%), Gaps = 3/42 (7%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTS 144
WG GL+S + G+ RG D NS +GA+TGA LA S
Sbjct: 69 WG---GLFSTIDCGLVRLRGKEDPWNSITSGALTGAVLAARS 107
>IM22_HUMAN (Q9Y584) Mitochondrial import inner membrane translocase
subunit Tim22
Length = 194
Score = 35.0 bits (79), Expect = 0.086
Identities = 14/38 (36%), Positives = 23/38 (59%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAAL 140
+ + ++S ++ RGT DWKNS ++G ITG A+
Sbjct: 129 FAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAI 166
>IM7B_MOUSE (Q9Z0V7) Mitochondrial import inner membrane translocase
subunit Tim17 B
Length = 172
Score = 34.3 bits (77), Expect = 0.15
Identities = 19/42 (45%), Positives = 24/42 (56%), Gaps = 3/42 (7%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTS 144
WG GL+S + G+ RG D NS +GA+TGA LA S
Sbjct: 69 WG---GLFSTIDCGLVRLRGKEDPWNSISSGALTGAVLAARS 107
>IM7A_MOUSE (Q9Z0V8) Mitochondrial import inner membrane translocase
subunit Tim17 A (Inner membrane preprotein translocase
Tim17a)
Length = 171
Score = 33.9 bits (76), Expect = 0.19
Identities = 27/71 (38%), Positives = 35/71 (49%), Gaps = 8/71 (11%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTSHEQIAQCAITG--- 159
WG GL+S + M + RG D NS +GA+TGA LA + N + A+ G
Sbjct: 69 WG---GLFSTIDCSMVQIRGKEDPWNSITSGALTGAILA--ARNGPVAMVGSAAMGGILL 123
Query: 160 AAISTAANLLT 170
A I A LLT
Sbjct: 124 ALIEGAGILLT 134
>IM7A_HUMAN (Q99595) Mitochondrial import inner membrane translocase
subunit Tim17 A (Inner membrane preprotein translocase
Tim17a)
Length = 171
Score = 33.9 bits (76), Expect = 0.19
Identities = 27/71 (38%), Positives = 35/71 (49%), Gaps = 8/71 (11%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTSHEQIAQCAITG--- 159
WG GL+S + M + RG D NS +GA+TGA LA + N + A+ G
Sbjct: 69 WG---GLFSMIDCSMVQVRGKEDPWNSITSGALTGAILA--ARNGPVAMVGSAAMGGILL 123
Query: 160 AAISTAANLLT 170
A I A LLT
Sbjct: 124 ALIEGAGILLT 134
>IM22_MOUSE (Q9CQ85) Mitochondrial import inner membrane translocase
subunit Tim22
Length = 194
Score = 32.7 bits (73), Expect = 0.43
Identities = 13/38 (34%), Positives = 22/38 (57%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAAL 140
+ + ++S ++ RG DWKNS ++G ITG A+
Sbjct: 129 FAIVGAMFSCTECLVESYRGKSDWKNSVISGCITGGAI 166
>IM23_HUMAN (O14925) Mitochondrial import inner membrane translocase
subunit Tim23
Length = 209
Score = 30.4 bits (67), Expect = 2.1
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 3/61 (4%)
Query: 104 GLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTSHEQIAQCAITGAAIS 163
G A LYS +++ RG D N+ AG +TG CT IA+ +TG ++
Sbjct: 132 GSLALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTG---GLRGIARGGLTGLTLT 188
Query: 164 T 164
+
Sbjct: 189 S 189
>IM23_BRARE (Q7T2P6) Mitochondrial import inner membrane translocase
subunit Tim23
Length = 208
Score = 30.4 bits (67), Expect = 2.1
Identities = 32/135 (23%), Positives = 54/135 (39%), Gaps = 12/135 (8%)
Query: 37 ESFVKAAGIGAVQAVSREAYFTV----IEGTGIDNAGGMPPEISGAKKNRFHGLRDQQ*I 92
+ F+ G + A+FT+ I G G+ +S + + R+ Q I
Sbjct: 58 DEFILPTGANKTRGRFELAFFTIGGCCITGAAFGTLNGLRMGLSETRDMPWSKPRNVQ-I 116
Query: 93 Y*GNGKESFQW----GLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACTSDNTS 148
++ W G A LYS +++ARG D N+ AG +TG T
Sbjct: 117 LNMVTRQGASWANTLGSVALLYSVFGVAIEKARGAEDDLNTVAAGTLTGMVFKSTG---G 173
Query: 149 HEQIAQCAITGAAIS 163
+ +A+ + G A+S
Sbjct: 174 LKGVARGGLIGLAMS 188
>GLS2_ARATH (Q9T0P4) Ferredoxin-dependent glutamate synthase 2,
chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT 2)
Length = 1629
Score = 29.6 bits (65), Expect = 3.6
Identities = 31/118 (26%), Positives = 50/118 (42%), Gaps = 5/118 (4%)
Query: 34 RIAESFVKAAGIGAVQAVSREAYFTVIEGTGIDNAGGMPP--EISGAKKNRFHGLRDQQ* 91
+++ V GIG V + +A +I+ +G D G P I A GL + Q
Sbjct: 1132 KVSVKLVSETGIGTVASGVAKANADIIQISGYDGGTGASPISSIKHAGGPWELGLAETQK 1191
Query: 92 IY*GNG-KESFQWGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALA--CTSDN 146
GNG +E + G SG+ + A G ++ +A TG +A C ++N
Sbjct: 1192 TLIGNGLRERVIIRVDGGFKSGVDVLIAAAMGADEYGFGTLAMIATGCIMARICHTNN 1249
>RBS1_HUMAN (P29558) RNA binding motif, single-stranded interacting
protein 1 (Single-stranded DNA-binding protein MSSP-1)
(Suppressor of CDC2 with RNA binding motif 2)
Length = 406
Score = 29.3 bits (64), Expect = 4.7
Identities = 17/49 (34%), Positives = 28/49 (56%), Gaps = 1/49 (2%)
Query: 7 LETRTLLDELSDFNKG-GLFDFGHPLVNRIAESFVKAAGIGAVQAVSRE 54
+ T+ +LD+ ++ KG G DF P + A S +KA+G+ A A +E
Sbjct: 89 VSTKAILDKTTNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQE 137
>IM23_MOUSE (Q9WTQ8) Mitochondrial import inner membrane translocase
subunit Tim23
Length = 209
Score = 29.3 bits (64), Expect = 4.7
Identities = 15/40 (37%), Positives = 20/40 (49%)
Query: 104 GLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACT 143
G A LYS +++ RG D N+ AG +TG CT
Sbjct: 132 GSLALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCT 171
>LIPB_TROWT (Q83G63) Lipoyltransferase (EC 2.3.1.-)
(Lipoyl-[acyl-carrier protein]-protein
-N-lipoyltransferase) (Lipoate-protein ligase B)
Length = 235
Score = 28.9 bits (63), Expect = 6.1
Identities = 12/33 (36%), Positives = 20/33 (60%)
Query: 137 GAALACTSDNTSHEQIAQCAITGAAISTAANLL 169
G AL C++D E+I C I+ A ++T + +L
Sbjct: 172 GFALNCSNDLAPFEKIVPCGISDAGVTTISRIL 204
>LIPB_TROW8 (Q83I07) Lipoyltransferase (EC 2.3.1.-)
(Lipoyl-[acyl-carrier protein]-protein
-N-lipoyltransferase) (Lipoate-protein ligase B)
Length = 225
Score = 28.9 bits (63), Expect = 6.1
Identities = 12/33 (36%), Positives = 20/33 (60%)
Query: 137 GAALACTSDNTSHEQIAQCAITGAAISTAANLL 169
G AL C++D E+I C I+ A ++T + +L
Sbjct: 162 GFALNCSNDLAPFEKIVPCGISDAGVTTISRIL 194
>IM23_RAT (O35093) Mitochondrial import inner membrane translocase
subunit Tim23
Length = 209
Score = 28.9 bits (63), Expect = 6.1
Identities = 15/40 (37%), Positives = 20/40 (49%)
Query: 104 GLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALACT 143
G A LYS +++ RG D N+ AG +TG CT
Sbjct: 132 GSLALLYSAFGVIIEKTRGAEDDFNTVAAGTMTGMLYKCT 171
>IM17_YEAST (P39515) Mitochondrial import inner membrane translocase
subunit TIM17 (Mitochondrial protein import protein 2)
(Mitochondrial inner membrane protein MIM17)
Length = 158
Score = 28.9 bits (63), Expect = 6.1
Identities = 15/39 (38%), Positives = 21/39 (53%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALA 141
+G+ GL+S +K R D N+ +AG TG ALA
Sbjct: 65 FGVWGGLFSTFDCAVKAVRKREDPWNAIIAGFFTGGALA 103
>ARC1_LACPL (P59391) N-acetyl-gamma-glutamyl-phosphate reductase 1
(EC 1.2.1.38) (AGPR 1) (N-acetyl-glutamate semialdehyde
dehydrogenase 1) (NAGSA dehydrogenase 1)
Length = 343
Score = 28.9 bits (63), Expect = 6.1
Identities = 14/37 (37%), Positives = 23/37 (61%), Gaps = 2/37 (5%)
Query: 37 ESFVKAAGIGAVQAVSREAYFTVIEGTGIDNAGGMPP 73
++ +K A AVQA ++ Y+ ++E TG+D GM P
Sbjct: 309 DNLLKGAAGQAVQAANK--YYQLVEDTGLDTLAGMWP 343
>IM17_SCHPO (P87130) Mitochondrial import inner membrane translocase
subunit tim17 (Mitochondrial protein import protein 2)
Length = 164
Score = 28.5 bits (62), Expect = 8.0
Identities = 15/39 (38%), Positives = 21/39 (53%)
Query: 103 WGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALA 141
+G+ GL+S +K R D N+ +AG TG ALA
Sbjct: 66 FGVWGGLFSTFDCAVKGVRRKEDPWNAIIAGFFTGGALA 104
>GLS1_ARATH (Q9ZNZ7) Ferredoxin-dependent glutamate synthase 1,
chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT 1)
Length = 1648
Score = 28.5 bits (62), Expect = 8.0
Identities = 31/118 (26%), Positives = 50/118 (42%), Gaps = 5/118 (4%)
Query: 34 RIAESFVKAAGIGAVQAVSREAYFTVIEGTGIDNAGGMPP--EISGAKKNRFHGLRDQQ* 91
+++ V AGIG V + + +I+ +G D G P I A GL +
Sbjct: 1156 KVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQ 1215
Query: 92 IY*GNG-KESFQWGLAAGLYSGLTYGMKEARGTHDWKNSAVAGAITGAALA--CTSDN 146
NG +E + GL SG+ M A G ++ ++A TG +A C ++N
Sbjct: 1216 TLIANGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNN 1273
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,405,229
Number of Sequences: 164201
Number of extensions: 759131
Number of successful extensions: 1392
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1375
Number of HSP's gapped (non-prelim): 22
length of query: 172
length of database: 59,974,054
effective HSP length: 102
effective length of query: 70
effective length of database: 43,225,552
effective search space: 3025788640
effective search space used: 3025788640
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC126780.8