BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126014.1 + phase: 0
(405 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YE76_AQUAE (O67453) Hypothetical protein AQ_1476 34 0.58
GIS4_YEAST (Q04233) Protein GIS4 34 0.58
Y552_HUMAN (O60299) Hypothetical protein KIAA0552 34 0.76
IF3T_TORCA (P23729) Type III intermediate filament 34 0.76
KNG_RAT (P08934) Kininogen precursor [Contains: Bradykinin] 33 1.7
VIME_RAT (P31000) Vimentin 32 2.2
VIME_MOUSE (P20152) Vimentin 32 2.2
VIME_MESAU (P02544) Vimentin 32 2.2
VIME_CRIGR (P48670) Vimentin (Fragment) 32 2.2
SP23_YEAST (P35210) SPT23 protein 32 2.2
VIME_PIG (P02543) Vimentin (Fragments) 32 2.9
VIME_CHICK (P09654) Vimentin 32 2.9
VIM2_CARAU (P48672) Vimentin A2 (Fragment) 32 2.9
CYAA_DICDI (Q03100) Adenylate cyclase, aggregation specific (EC ... 32 2.9
SER2_GALME (O96615) Sericin-2 (Silk gum protein 2) (Fragment) 32 3.8
RA50_PYRHO (O58687) DNA double-strand break repair rad50 ATPase 32 3.8
MYSP_BOOMI (Q86RN8) Paramyosin 32 3.8
YSM5_CAEEL (Q10125) Hypothetical protein F52C9.5 in chromosome III 31 4.9
YGG6_YEAST (P53165) Hypothetical 72.9 kDa protein in RPB9-ALG2 i... 31 4.9
VIME_HUMAN (P08670) Vimentin 31 4.9
>YE76_AQUAE (O67453) Hypothetical protein AQ_1476
Length = 180
Score = 34.3 bits (77), Expect = 0.58
Identities = 21/87 (24%), Positives = 46/87 (52%), Gaps = 10/87 (11%)
Query: 305 HERIMEESKKRDRKNACGLLREIQQIEKCVRVMSDLVDSAHFPLTEEKEGEVRQKVHEVS 364
+ER+++E +K + A L EI ++ + + + + H +KE E+ +K+H++
Sbjct: 97 YERLLQERQKLIER-AYKLSEEIYELRRKYEALREEEEKLH-----QKEDEIEEKIHKLK 150
Query: 365 K----VCDALKDGLDPLERQVREVFHR 387
K + + LK ++ L R+ RE+ +
Sbjct: 151 KEYRALLNELKGLIEELNRKAREIIEK 177
>GIS4_YEAST (Q04233) Protein GIS4
Length = 774
Score = 34.3 bits (77), Expect = 0.58
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 7/65 (10%)
Query: 196 HQHGNSHSNN-----NINTYQHRSMGQFRSLSWSVSRTWSAARQLQAIGNNINPPRANDL 250
H H +H N+ N+N +Q + +F S SW V + A N+I PP +
Sbjct: 105 HSHCYNHENHYLLTDNLNNFQDPTFLEFASTSWQVQKNSKALNNNNR--NSIPPPTISSS 162
Query: 251 MATNG 255
A+NG
Sbjct: 163 KASNG 167
>Y552_HUMAN (O60299) Hypothetical protein KIAA0552
Length = 673
Score = 33.9 bits (76), Expect = 0.76
Identities = 22/77 (28%), Positives = 44/77 (56%), Gaps = 3/77 (3%)
Query: 309 MEESKKRDRKNACGLLREIQQIEKCVRVMSDLVDSAHF-PLTEEKEGEVRQKVHEVSKVC 367
+++ KK+ ++ A L+R+ +++E +V + + A F P EE + EV QK E+S +
Sbjct: 396 LQQDKKQLQEEAARLMRQREELED--KVAACQKEQADFLPRIEETKWEVCQKAGEISLLK 453
Query: 368 DALKDGLDPLERQVREV 384
LKD + +++ E+
Sbjct: 454 QQLKDSQADVSQKLSEI 470
>IF3T_TORCA (P23729) Type III intermediate filament
Length = 458
Score = 33.9 bits (76), Expect = 0.76
Identities = 21/74 (28%), Positives = 38/74 (50%), Gaps = 5/74 (6%)
Query: 337 MSDLVDSAH-----FPLTEEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRS 391
++D+ DSA L +++ E R++V ++ DALK + LERQ+++V R
Sbjct: 289 LADITDSASRNNDALRLAKQENNEYRRQVQSLTCEIDALKGTNESLERQMQDVEDRYNME 348
Query: 392 KTEGLDSLGRPNNE 405
T D++ +E
Sbjct: 349 TTNAQDTISHLEDE 362
>KNG_RAT (P08934) Kininogen precursor [Contains: Bradykinin]
Length = 639
Score = 32.7 bits (73), Expect = 1.7
Identities = 37/122 (30%), Positives = 51/122 (41%), Gaps = 11/122 (9%)
Query: 143 HQRSIGEGQFRRAKKALIDLSISMLDDGKESNASVAH-RNRSFGRSNG-GRDRDHHQHGN 200
HQ+ G G + K L DL +DG + V H + G +G G RD +H N
Sbjct: 452 HQKPHGLGHGHQLK--LDDLK-QQREDGYDHRHPVGHGHGQRHGHGHGHGHGRD--KHTN 506
Query: 201 SHSNNNINTYQHRSMGQFRSLSWSVSRTWSAARQLQAIGNNINPPRANDLMATNGLAMSV 260
NN +T Q R+ S S T S Q + G +NPP A + + G S
Sbjct: 507 KDKNNVKHTDQRRA----PLTSSSEDNTTSTQIQGRTEGFTLNPPLAQPAVISRGFQDSG 562
Query: 261 YT 262
+T
Sbjct: 563 FT 564
>VIME_RAT (P31000) Vimentin
Length = 465
Score = 32.3 bits (72), Expect = 2.2
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 312 KQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDE 367
>VIME_MOUSE (P20152) Vimentin
Length = 465
Score = 32.3 bits (72), Expect = 2.2
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 312 KQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDE 367
>VIME_MESAU (P02544) Vimentin
Length = 464
Score = 32.3 bits (72), Expect = 2.2
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 311 KQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDE 366
>VIME_CRIGR (P48670) Vimentin (Fragment)
Length = 448
Score = 32.3 bits (72), Expect = 2.2
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 295 KQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDE 350
>SP23_YEAST (P35210) SPT23 protein
Length = 1082
Score = 32.3 bits (72), Expect = 2.2
Identities = 14/55 (25%), Positives = 28/55 (50%)
Query: 172 ESNASVAHRNRSFGRSNGGRDRDHHQHGNSHSNNNINTYQHRSMGQFRSLSWSVS 226
++N ++ + NR F N + +H + H +NN + HRS+ + S ++ S
Sbjct: 388 KTNTAMMNNNRHFPSPNSSSEDSNHSFSDIHFSNNNDNNLHRSLDSWSSTGFNSS 442
>VIME_PIG (P02543) Vimentin (Fragments)
Length = 275
Score = 32.0 bits (71), Expect = 2.9
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 122 KQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDE 177
>VIME_CHICK (P09654) Vimentin
Length = 459
Score = 32.0 bits (71), Expect = 2.9
Identities = 16/56 (28%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R+++ ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 306 KQEANEYRRQIQSLTCEVDALKGSNESLERQMREMEENFAVEAANYQDTIGRLQDE 361
>VIM2_CARAU (P48672) Vimentin A2 (Fragment)
Length = 243
Score = 32.0 bits (71), Expect = 2.9
Identities = 16/54 (29%), Positives = 29/54 (53%)
Query: 348 LTEEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGR 401
L +++ E R++V ++ DALK + LERQ+RE+ + D++ R
Sbjct: 88 LAKQEANEYRRQVQALTCEVDALKGTNESLERQMREIEENFAIESSSSQDNIAR 141
>CYAA_DICDI (Q03100) Adenylate cyclase, aggregation specific (EC
4.6.1.1) (ATP pyrophosphate-lyase) (Adenylyl cyclase)
Length = 1407
Score = 32.0 bits (71), Expect = 2.9
Identities = 24/94 (25%), Positives = 44/94 (46%), Gaps = 5/94 (5%)
Query: 152 FRRAKKALIDLSISMLDDGKESNASVAHRNRSFGRSNGGRDRDHHQHGNSHSNNNINTYQ 211
+R KK + S+ K NA ++S + + +++ + N+++NNN+N
Sbjct: 725 YREKKKKDLSCDFSI---NKVLNAETIE-SKSNNNNYNNNNYNNNNYNNNYNNNNLNNNS 780
Query: 212 HRSMGQFRSLSWSVSRTWSAARQLQAIGNNINPP 245
+ + ++ S S S S A + Q NN NPP
Sbjct: 781 NNNNNEYGSSSSSSSVLGEAVTE-QIDCNNTNPP 813
>SER2_GALME (O96615) Sericin-2 (Silk gum protein 2) (Fragment)
Length = 220
Score = 31.6 bits (70), Expect = 3.8
Identities = 26/97 (26%), Positives = 45/97 (45%), Gaps = 5/97 (5%)
Query: 173 SNASVAHRNRSFGRSNGGRDRDHHQHGNSHSNNNINTYQHRSMGQFRSLSWSVSRTW--- 229
+N S + RN S G S+ GNS +++ N+ +G S S S +
Sbjct: 41 NNTSGSSRNNSSGSSSSNNSSSGSSAGNSSGSSSSNSSGSSGIGSLSRNSSSSSSSQAAG 100
Query: 230 -SAARQLQAIGNNINPPRANDLMATNGLAMSVYTMNS 265
S++R++ A G++ + +N A N A S T+N+
Sbjct: 101 SSSSRRVSADGSSSSSSASNSAAAQNS-ASSSETINA 136
>RA50_PYRHO (O58687) DNA double-strand break repair rad50 ATPase
Length = 879
Score = 31.6 bits (70), Expect = 3.8
Identities = 21/84 (25%), Positives = 41/84 (48%)
Query: 311 ESKKRDRKNACGLLREIQQIEKCVRVMSDLVDSAHFPLTEEKEGEVRQKVHEVSKVCDAL 370
ES K +R+ E+ +IE + ++ ++ ++K E R+K+ ++S L
Sbjct: 619 ESLKDEREELDKAFEELAKIETDIEKVTSQLNELQRKFDQKKYEEKREKMMKLSMEIKGL 678
Query: 371 KDGLDPLERQVREVFHRIVRSKTE 394
+ L+ LER+ E+ I + K E
Sbjct: 679 ETKLEELERRRDEIKSTIEKLKEE 702
>MYSP_BOOMI (Q86RN8) Paramyosin
Length = 873
Score = 31.6 bits (70), Expect = 3.8
Identities = 21/86 (24%), Positives = 47/86 (54%), Gaps = 4/86 (4%)
Query: 319 NACGLLREIQQIEKCVRVMSDLVDSAHFPLTEEKEGEVRQKVHEVSKV---CDALKDGLD 375
+A L + + Q+EK + V+ L E+ + E+RQKV E+ K+ + +++ D
Sbjct: 360 HAQNLEKRVAQLEKLNIDLKSKVEELTI-LLEQSQRELRQKVAEIQKLQHEYEKMREQRD 418
Query: 376 PLERQVREVFHRIVRSKTEGLDSLGR 401
L+R+ +++ + +K++ D++ R
Sbjct: 419 ALQRENKKLVDDLSEAKSQLADAIRR 444
>YSM5_CAEEL (Q10125) Hypothetical protein F52C9.5 in chromosome III
Length = 498
Score = 31.2 bits (69), Expect = 4.9
Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 3/80 (3%)
Query: 314 KRDRKNACGLLREIQQIEKCVRVMSDLVDSAHFPLTEEKEGEVRQKVHE--VSKVCDALK 371
K+ R+ C +I+ + VR D +DS ++ ++ GE+ HE DA +
Sbjct: 370 KQTRRKICANFNQIEDCAEEVRDCVDEIDSTDMRMSIKRAGELENNDHEQIEDNNTDASE 429
Query: 372 DGLDPLERQVREVFHRIVRS 391
D + P + ++ EV +I R+
Sbjct: 430 DPV-PTKEEIAEVKQKIRRT 448
>YGG6_YEAST (P53165) Hypothetical 72.9 kDa protein in RPB9-ALG2
intergenic region
Length = 657
Score = 31.2 bits (69), Expect = 4.9
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 2/76 (2%)
Query: 139 YALDHQRSIGEGQFRRAKKA-LIDLSISMLDDGKESNASVAHRNRSFGRS-NGGRDRDHH 196
Y +HQ IG +RAK+ L L + + K+ RS R+ NGG+ +
Sbjct: 274 YHREHQTKIGAAAEKRAKQQELQKLQKQIQKEQKKHTQQQKQGQRSKQRNVNGGKSAKNG 333
Query: 197 QHGNSHSNNNINTYQH 212
H+ NNIN H
Sbjct: 334 GKSTVHNGNNINEIGH 349
>VIME_HUMAN (P08670) Vimentin
Length = 465
Score = 31.2 bits (69), Expect = 4.9
Identities = 17/56 (30%), Positives = 30/56 (53%)
Query: 350 EEKEGEVRQKVHEVSKVCDALKDGLDPLERQVREVFHRIVRSKTEGLDSLGRPNNE 405
+++ E R++V ++ DALK + LERQ+RE+ D++GR +E
Sbjct: 312 KQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDE 367
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,290,780
Number of Sequences: 164201
Number of extensions: 1842044
Number of successful extensions: 5828
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 5766
Number of HSP's gapped (non-prelim): 66
length of query: 405
length of database: 59,974,054
effective HSP length: 113
effective length of query: 292
effective length of database: 41,419,341
effective search space: 12094447572
effective search space used: 12094447572
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 67 (30.4 bits)
Medicago: description of AC126014.1