Medicago
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC126012.10 + phase: 0 /pseudo
         (498 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YKF9_YEAST (P35728) Hypothetical 49.6 kDa protein in FBA1-TOA2 i...    87  8e-17
COP1_ARATH (P43254) Ubiquitin ligase protein COP1 (EC 6.3.2.-) (...    54  9e-07
COP1_PEA (P93471) Ubiquitin ligase protein COP1 (EC 6.3.2.-) (Co...    50  1e-05
RA18_HUMAN (Q9NS91) Postreplication repair protein RAD18 (hRAD18...    48  5e-05
RA18_MOUSE (Q9QXK2) Postreplication repair protein RAD18 (mRAD18Sc)    41  0.008
NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B)            40  0.018
GRP2_NICSY (P27484) Glycine-rich protein 2                             40  0.018
NF7O_XENLA (Q91431) Nuclear factor 7, ovary (xnf7-O)                   39  0.041
BRD3_MOUSE (Q8K2F0) Bromodomain-containing protein 3 (Bromodomai...    39  0.041
TM22_HUMAN (Q8IYM9) Tripartite motif-containing protein 22 (RING...    38  0.069
UFD2_SCHPO (Q9HE05) Ubiquitin conjugation factor E4 (Ubiquitin f...    37  0.12
RNG1_HUMAN (Q06587) Polycomb complex protein RING1 (RNF1)              37  0.12
BMI1_MOUSE (P25916) Polycomb complex protein BMI-1                     37  0.12
BMI1_HUMAN (P35226) Polycomb complex protein BMI-1                     37  0.12
BMI1_FELCA (Q9TST0) Polycomb complex protein BMI-1                     37  0.12
NUCL_XENLA (P20397) Nucleolin (Protein C23)                            37  0.15
ME18_MOUSE (P23798) DNA-binding protein Mel-18 (RING finger prot...    37  0.15
ME18_HUMAN (P35227) DNA-binding protein Mel-18 (RING finger prot...    37  0.15
IF38_SCHPO (O14164) Probable eukaryotic translation initiation f...    37  0.15
GSA_MYCTU (P63506) Glutamate-1-semialdehyde 2,1-aminomutase (EC ...    37  0.15

>YKF9_YEAST (P35728) Hypothetical 49.6 kDa protein in FBA1-TOA2
           intergenic region
          Length = 441

 Score = 87.4 bits (215), Expect = 8e-17
 Identities = 65/257 (25%), Positives = 111/257 (42%), Gaps = 35/257 (13%)

Query: 38  SKADEDSKIKALIDTPALDWQQQGSDFGAGRGFRRGAVGGRIGGGRGFGLERKTPPEGYV 97
           S   E+ +I ++  T    W+Q   +  A       +   +       G     PP GY+
Sbjct: 126 SGTTEEERIASMFATQENQWEQTQEEMSAATPVFFKSQTNKNSAQENEG----PPPPGYM 181

Query: 98  CHRCKVSGHFIQHCPTNGDSTFDIKKVRQPTGIPRSMLM---VNPQGSYALQNGSVAVLK 154
           C+RC    H+I++CPTN D  F+ K++R+ TGIP+  L    ++P+     +     ++ 
Sbjct: 182 CYRCGGRDHWIKNCPTNSDPNFEGKRIRRTTGIPKKFLKSIEIDPETMTPEEMAQRKIMI 241

Query: 155 PNEAAFDKEMEGLSS---------TRSVG------------DLPPELHCPLCNNVMKDAV 193
            +E  F  ++E   S          R +             DLP +L CPL   +++  V
Sbjct: 242 TDEGKFVVQVEDKQSWEDYQRKRENRQIDGDETIWRKGHFKDLPDDLKCPLTGGLLRQPV 301

Query: 194 LTSKCCFKSFCDKCIRNYIM-SKSACV-CLATNILADDLLPNKTLRDAIHRILES----- 246
            TSKCC   F  + + N ++ S   C  C   +IL D L+P++     +   L+      
Sbjct: 302 KTSKCCNIDFSKEALENALVESDFVCPNCETRDILLDSLVPDQDKEKEVETFLKKQEELH 361

Query: 247 GNSSTENAGSTYQVQDM 263
           G+S   N   T +++ M
Sbjct: 362 GSSKDGNQPETKKMKLM 378


>COP1_ARATH (P43254) Ubiquitin ligase protein COP1 (EC 6.3.2.-)
           (Constitutive photomorphogenesis protein 1)
          Length = 675

 Score = 53.9 bits (128), Expect = 9e-07
 Identities = 33/106 (31%), Positives = 53/106 (49%), Gaps = 6/106 (5%)

Query: 165 EGLSSTRSVG--DLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLA 222
           +G S    +G  DL  +L CP+C  ++KDA LT+  C  SFC  CI  ++ +KS C C +
Sbjct: 33  DGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTA--CGHSFCYMCIITHLRNKSDCPCCS 90

Query: 223 TNILADDLLPNKTLRDAIHRILESGNSSTENAGSTYQVQDMLSSRC 268
            ++  + L PN  L   + +   S    ++ A    Q ++ L   C
Sbjct: 91  QHLTNNQLYPNFLLDKLLKK--TSARHVSKTASPLDQFREALQRGC 134


>COP1_PEA (P93471) Ubiquitin ligase protein COP1 (EC 6.3.2.-)
           (Constitutive photomorphogenesis protein 1)
          Length = 672

 Score = 50.4 bits (119), Expect = 1e-05
 Identities = 26/80 (32%), Positives = 41/80 (50%), Gaps = 2/80 (2%)

Query: 172 SVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDLL 231
           ++ DL  +  CP+C  ++KDA LT+  C  SFC  CI  ++ +KS C C    +   +L 
Sbjct: 37  TMSDLDKDFLCPICMQIIKDAFLTA--CGHSFCYMCIITHLRNKSDCPCCGHYLTNSNLF 94

Query: 232 PNKTLRDAIHRILESGNSST 251
           PN  L   + +  +   S T
Sbjct: 95  PNFLLDKLLKKTSDRQISKT 114


>RA18_HUMAN (Q9NS91) Postreplication repair protein RAD18 (hRAD18)
           (hHR18)
          Length = 495

 Score = 48.1 bits (113), Expect = 5e-05
 Identities = 42/201 (20%), Positives = 76/201 (36%), Gaps = 29/201 (14%)

Query: 166 GLSSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNI 225
           GL+  +++ DL   L C +C      A++  +C   ++C  CIR ++  K+ C      +
Sbjct: 12  GLAVMKTIDDL---LRCGICFEYFNIAMIIPQCSH-NYCSLCIRKFLSYKTQCPTCCVTV 67

Query: 226 LADDLLPNKTLRDAIHRILESGNSSTENAGSTYQVQDMLSSRCPQPKIPSPTSSATSKGE 285
              DL  N+ L + +  +         N    + +Q  L S    P   S  + A     
Sbjct: 68  TEPDLKNNRILDELVKSL---------NFARNHLLQFALESPAKSPASSSSKNLAVKVYT 118

Query: 286 PKVSQ--------------VNEGMANIQEIVAE--RKEVSATQQVSEQVKIPRAAVVSEV 329
           P  S+              + E   +  E++ +  + + S  ++ S   K      V E+
Sbjct: 119 PVASRQSLKQGSRLMDNFLIREMSGSTSELLIKENKSKFSPQKEASPAAKTKETRSVEEI 178

Query: 330 THESMSVKEPEPASQGSAKLV 350
             +    K PEP S  + K V
Sbjct: 179 APDPSEAKRPEPPSTSTLKQV 199


>RA18_MOUSE (Q9QXK2) Postreplication repair protein RAD18 (mRAD18Sc)
          Length = 509

 Score = 40.8 bits (94), Expect = 0.008
 Identities = 34/135 (25%), Positives = 58/135 (42%), Gaps = 18/135 (13%)

Query: 166 GLSSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNI 225
           GL+  +++ DL   L C +C      AV+  +C   ++C  CIR ++  K+ C      +
Sbjct: 12  GLAVMKTIDDL---LRCGICFEYFNIAVIIPQCSH-NYCSLCIRKFLSYKTQCPTCCVAV 67

Query: 226 LADDLLPNKTLRDAIHRILESGNSSTENAGSTYQVQDMLSSRCPQPKIPSPTSSATSKGE 285
              DL  N+ L + +          + N   T+ +Q  L S    P I SP SS + K  
Sbjct: 68  TEPDLRNNRLLDELV---------KSMNFARTHLLQFALES----PPI-SPVSSTSKKVV 113

Query: 286 PKVSQVNEGMANIQE 300
            KV   +     +++
Sbjct: 114 VKVHNADAAQHPVKQ 128


>NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B)
          Length = 609

 Score = 39.7 bits (91), Expect = 0.018
 Identities = 22/69 (31%), Positives = 35/69 (49%), Gaps = 4/69 (5%)

Query: 154 KPNEAAFDKE--MEGLSSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNY 211
           +P +A  +++   +  SS  + GD   EL CPLC  + KD V+ +  C  +FC  CI   
Sbjct: 115 EPKKAKVEEKDASKNASSLGAAGDFAEELTCPLCVELFKDPVMVA--CGHNFCRSCIDKA 172

Query: 212 IMSKSACVC 220
              +S+  C
Sbjct: 173 WEGQSSFAC 181


>GRP2_NICSY (P27484) Glycine-rich protein 2
          Length = 214

 Score = 39.7 bits (91), Expect = 0.018
 Identities = 22/55 (40%), Positives = 26/55 (47%), Gaps = 2/55 (3%)

Query: 61  GSDFGAGRGFRRGAVGGRIGGGRGFGLERKTPPEGYVCHRCKVSGHFIQHCPTNG 115
           G   G G G R G  GG  GGG G+G        G  C +C  SGHF + C  +G
Sbjct: 124 GGGGGYGGGSRYGGGGGGYGGGGGYGGGGSGGGSG--CFKCGESGHFARDCSQSG 176



 Score = 34.7 bits (78), Expect = 0.59
 Identities = 17/43 (39%), Positives = 21/43 (48%), Gaps = 7/43 (16%)

Query: 73  GAVGGRIGGGRGFGLERKTPPEGYVCHRCKVSGHFIQHCPTNG 115
           G  GGR GGG G G        G  C++C   GHF + C + G
Sbjct: 178 GGGGGRFGGGGGGG-------GGGGCYKCGEDGHFARECTSGG 213


>NF7O_XENLA (Q91431) Nuclear factor 7, ovary (xnf7-O)
          Length = 610

 Score = 38.5 bits (88), Expect = 0.041
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 2/53 (3%)

Query: 168 SSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVC 220
           +S  + GD   EL CPLC  + KD V+ +  C  +FC  CI      +S+  C
Sbjct: 132 ASLGAAGDFAEELTCPLCVELFKDPVMVA--CGHNFCRSCIDKVWEGQSSFAC 182


>BRD3_MOUSE (Q8K2F0) Bromodomain-containing protein 3
           (Bromodomain-containing FSH-like protein FSRG2)
          Length = 726

 Score = 38.5 bits (88), Expect = 0.041
 Identities = 23/103 (22%), Positives = 45/103 (43%), Gaps = 9/103 (8%)

Query: 271 PKIPSPTSSATSKGEPKVSQVNEGMANIQEIVAERKEVSATQQVSEQVKIPRAAVVSEVT 330
           P +P+PT+   SKG        E  ++     +E +  +   ++ EQ+K           
Sbjct: 422 PALPAPTAPIVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLK---------AV 472

Query: 331 HESMSVKEPEPASQGSAKLVEEEVQQKLVPTDGGKKKKKKKVR 373
           HE ++     P ++   K  ++E ++K    D  K+K+K K +
Sbjct: 473 HEQLAALSQAPVNKPKKKKEKKEKEKKKKDKDKDKEKEKHKAK 515


>TM22_HUMAN (Q8IYM9) Tripartite motif-containing protein 22 (RING
           finger protein 94) (50 kDa stimulated trans-acting
           factor) (Staf-50)
          Length = 498

 Score = 37.7 bits (86), Expect = 0.069
 Identities = 25/85 (29%), Positives = 41/85 (47%), Gaps = 9/85 (10%)

Query: 175 DLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCI-----RNYIMSK--SACVCLATNILA 227
           D+  E+ CP+C  ++ + +  S  C  SFC  CI      + I+S+  S+C    T    
Sbjct: 8   DIEKEVTCPICLELLTEPL--SLDCGHSFCQACITAKIKESVIISRGESSCPVCQTRFQP 65

Query: 228 DDLLPNKTLRDAIHRILESGNSSTE 252
            +L PN+ L + + R+ E   S  E
Sbjct: 66  GNLRPNRHLANIVERVKEVKMSPQE 90


>UFD2_SCHPO (Q9HE05) Ubiquitin conjugation factor E4 (Ubiquitin fusion
            degradation protein 2) (UB fusion protein 2)
          Length = 1010

 Score = 37.0 bits (84), Expect = 0.12
 Identities = 23/83 (27%), Positives = 41/83 (48%), Gaps = 1/83 (1%)

Query: 164  MEGLSSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLAT 223
            ++  +    +GD+P     PL   +MKD V+  +    S     I+ +++S +      T
Sbjct: 919  LQEATEEEDMGDIPDYFLDPLMFTIMKDPVVLPRSGI-SIDRSTIKAHLLSDATDPFNRT 977

Query: 224  NILADDLLPNKTLRDAIHRILES 246
             +  DD+ PN TLR+ I+  L+S
Sbjct: 978  PLTLDDVTPNDTLREEINTFLKS 1000


>RNG1_HUMAN (Q06587) Polycomb complex protein RING1 (RNF1)
          Length = 377

 Score = 37.0 bits (84), Expect = 0.12
 Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 3/85 (3%)

Query: 164 MEGLSSTRSVGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMS--KSACVCL 221
           M+G     S   L  EL CP+C +++K+  +T+K C   FC  CI   + S  K    C 
Sbjct: 1   MDGTEIAVSPRSLHSELMCPICLDMLKN-TMTTKECLHRFCSDCIVTALRSGNKECPTCR 59

Query: 222 ATNILADDLLPNKTLRDAIHRILES 246
              +    L P+      I +I  S
Sbjct: 60  KKLVSKRSLRPDPNFDALISKIYPS 84


>BMI1_MOUSE (P25916) Polycomb complex protein BMI-1
          Length = 324

 Score = 37.0 bits (84), Expect = 0.12
 Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 5/76 (6%)

Query: 173 VGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDLLP 232
           + +L P L C LC     DA    +C   SFC  CI  Y+ +   C      +     L 
Sbjct: 9   ITELNPHLMCVLCGGYFIDATTIIEC-LHSFCKTCIVRYLETSKYCPICDVQVHKTRPLL 67

Query: 233 N----KTLRDAIHRIL 244
           N    KTL+D +++++
Sbjct: 68  NIRSDKTLQDIVYKLV 83


>BMI1_HUMAN (P35226) Polycomb complex protein BMI-1
          Length = 326

 Score = 37.0 bits (84), Expect = 0.12
 Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 5/76 (6%)

Query: 173 VGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDLLP 232
           + +L P L C LC     DA    +C   SFC  CI  Y+ +   C      +     L 
Sbjct: 9   ITELNPHLMCVLCGGYFIDATTIIEC-LHSFCKTCIVRYLETSKYCPICDVQVHKTRPLL 67

Query: 233 N----KTLRDAIHRIL 244
           N    KTL+D +++++
Sbjct: 68  NIRSDKTLQDIVYKLV 83


>BMI1_FELCA (Q9TST0) Polycomb complex protein BMI-1
          Length = 326

 Score = 37.0 bits (84), Expect = 0.12
 Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 5/76 (6%)

Query: 173 VGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDLLP 232
           + +L P L C LC     DA    +C   SFC  CI  Y+ +   C      +     L 
Sbjct: 9   ITELNPHLMCVLCGGYFIDATTIIEC-LHSFCKTCIVRYLETSKYCPICDVQVHKTRPLL 67

Query: 233 N----KTLRDAIHRIL 244
           N    KTL+D +++++
Sbjct: 68  NIRSDKTLQDIVYKLV 83


>NUCL_XENLA (P20397) Nucleolin (Protein C23)
          Length = 650

 Score = 36.6 bits (83), Expect = 0.15
 Identities = 27/60 (45%), Positives = 31/60 (51%), Gaps = 6/60 (10%)

Query: 33  DAPLTSKADEDSKI---KALID--TPALDWQQQG-SDFGAGRGFRRGAVGGRIGGGRGFG 86
           DA    +A ED +I   K  +D   P  D Q+ G   FG G GFR G  G   GGGRGFG
Sbjct: 552 DAKAAKEAMEDGEIDGNKVTLDFAKPKGDSQRGGRGGFGRGGGFRGGRGGRGGGGGRGFG 611


>ME18_MOUSE (P23798) DNA-binding protein Mel-18 (RING finger protein
           110) (Zinc finger protein 144)
          Length = 342

 Score = 36.6 bits (83), Expect = 0.15
 Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 5/76 (6%)

Query: 173 VGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILAD---- 228
           + +L P L C LC     DA    +C   SFC  CI  Y+ +   C      +       
Sbjct: 9   ITELNPHLMCALCGGYFIDATTIVEC-LHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLL 67

Query: 229 DLLPNKTLRDAIHRIL 244
            +  +KTL+D +++++
Sbjct: 68  SIRSDKTLQDIVYKLV 83


>ME18_HUMAN (P35227) DNA-binding protein Mel-18 (RING finger protein
           110) (Zinc finger protein 144)
          Length = 344

 Score = 36.6 bits (83), Expect = 0.15
 Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 5/76 (6%)

Query: 173 VGDLPPELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILAD---- 228
           + +L P L C LC     DA    +C   SFC  CI  Y+ +   C      +       
Sbjct: 9   ITELNPHLMCALCGGYFIDATTIVEC-LHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLL 67

Query: 229 DLLPNKTLRDAIHRIL 244
            +  +KTL+D +++++
Sbjct: 68  SIRSDKTLQDIVYKLV 83


>IF38_SCHPO (O14164) Probable eukaryotic translation initiation
           factor 3 93 kDa subunit (eIF3 p93)
          Length = 918

 Score = 36.6 bits (83), Expect = 0.15
 Identities = 38/135 (28%), Positives = 62/135 (45%), Gaps = 3/135 (2%)

Query: 245 ESGNSSTEN--AGSTYQVQDMLSSRCPQPKIPSPTSSAT-SKGEPKVSQVNEGMANIQEI 301
           ES +SS EN    S  + QD  SS     +  S +SS + S  E   S+ +E     ++ 
Sbjct: 15  ESVDSSEENRLTSSRLKKQDDSSSEEESSEEESASSSESESSEEESESEESEVEVPKKKA 74

Query: 302 VAERKEVSATQQVSEQVKIPRAAVVSEVTHESMSVKEPEPASQGSAKLVEEEVQQKLVPT 361
           VA  ++  +  + SE+ +   +   SEV+ ES S  E E  S+  ++  EE  + +    
Sbjct: 75  VAASEDSESDSESSEEEEETESEEDSEVSDESESESESESESEEESESEEESDESERSGP 134

Query: 362 DGGKKKKKKKVRMPA 376
               KK +K+   PA
Sbjct: 135 SSFLKKPEKEEAKPA 149


>GSA_MYCTU (P63506) Glutamate-1-semialdehyde 2,1-aminomutase (EC
           5.4.3.8) (GSA) (Glutamate-1-semialdehyde
           aminotransferase) (GSA-AT)
          Length = 462

 Score = 36.6 bits (83), Expect = 0.15
 Identities = 24/86 (27%), Positives = 38/86 (43%), Gaps = 1/86 (1%)

Query: 268 CPQPKIPSPTSSATSKGEPKVSQVNEGMANIQEIVAERKEVSATQQVSEQVKIPRAAVVS 327
           C  P+ P+   S +S+G P    V  G A    IV    ++ A QQ   +     AAV++
Sbjct: 177 CDDPQRPASPRSQSSRGLPSSPGVT-GAAAADTIVLPYNDIDAVQQTFARFGEQIAAVIT 235

Query: 328 EVTHESMSVKEPEPASQGSAKLVEEE 353
           E +  +M V  P P    + + +  E
Sbjct: 236 EASPGNMGVVPPGPGFNAALRAITAE 261


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.320    0.135    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,941,081
Number of Sequences: 164201
Number of extensions: 2612956
Number of successful extensions: 9715
Number of sequences better than 10.0: 122
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 115
Number of HSP's that attempted gapping in prelim test: 9503
Number of HSP's gapped (non-prelim): 273
length of query: 498
length of database: 59,974,054
effective HSP length: 114
effective length of query: 384
effective length of database: 41,255,140
effective search space: 15841973760
effective search space used: 15841973760
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)


Medicago: description of AC126012.10