BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126009.2 + phase: 0
(184 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HS90_SCHPO (P41887) Heat shock protein 90 homolog 41 0.002
LEF_BACAN (P15917) Lethal factor precursor (EC 3.4.24.83) (LF) (... 35 0.13
YKZ6_YEAST (P36112) Hypothetical 61.1 kDa protein in YPT52-DBP7 ... 34 0.22
PP71_HCMVA (P06726) 71 kDa upper matrix phosphoprotein (PP71) 33 0.37
MAD1_KLUDE (Q874L7) Spindle assembly checkpoint component MAD1 (... 33 0.37
HS11_LYCES (P30221) 17.8 kDa class I heat shock protein 32 0.82
SP21_STIAU (Q06823) Spore protein SP21 32 1.1
HS11_HELAN (P30693) 17.6 kDa class I heat shock protein 32 1.1
VATI_PYRHO (O57721) V-type ATP synthase subunit I (EC 3.6.3.14) ... 31 1.4
TIG_AQUAE (O67358) Trigger factor (TF) 31 1.4
SYRC_YEAST (Q05506) Arginyl-tRNA synthetase, cytoplasmic (EC 6.1... 31 1.4
HS13_ARATH (P19037) 18.2 kDa class I heat shock protein (HSP 18.2) 31 1.4
SYA_CHLCV (Q821R2) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-... 31 1.8
SCPA_STRMU (Q7ZAK8) Segregation and condensation protein A 31 1.8
HOS4_YEAST (P40480) Protein HOS4 31 1.8
GRPE_CLOTE (Q892Q9) GrpE protein (HSP-70 cofactor) 31 1.8
E2BD_YEAST (P12754) Translation initiation factor eIF-2B delta s... 31 1.8
ARS2_MOUSE (Q99MR6) Arsenite-resistance protein 2 31 1.8
YAH3_YEAST (P39706) Hypothetical 48.7 kDa Trp-Asp repeats contai... 30 2.4
HS82_YEAST (P02829) ATP-dependent molecular chaperone HSP82 (Hea... 30 2.4
>HS90_SCHPO (P41887) Heat shock protein 90 homolog
Length = 704
Score = 40.8 bits (94), Expect = 0.002
Identities = 36/134 (26%), Positives = 65/134 (47%), Gaps = 20/134 (14%)
Query: 33 LPGNGERPFDGTKWKRFKKEFELPKYCNEDAIR------GNFMQNILSVVLPKKVDL-IP 85
L +G R GT+ + F KE +L +Y E I+ F+ + +V+ ++V+ +P
Sbjct: 161 LDTDGPRLLRGTEIRLFMKEDQL-QYLEEKTIKDTVKKHSEFISYPIQLVVTREVEKEVP 219
Query: 86 QEEQ------EEEEKIPELEDLDKYQEKNTYKSLGFGGRDREEEIGTLSEYTYRTDNKFG 139
+EE+ EE++K P++E++D EK K+ + +E T +E +T +
Sbjct: 220 EEEETEEVKNEEDDKAPKIEEVDDESEKKEKKT------KKVKETTTETEELNKTKPIWT 273
Query: 140 ENDVETTREVALKF 153
N E T+E F
Sbjct: 274 RNPSEVTKEEYASF 287
>LEF_BACAN (P15917) Lethal factor precursor (EC 3.4.24.83) (LF)
(Anthrax lethal toxin endopeptidase component)
Length = 809
Score = 34.7 bits (78), Expect = 0.13
Identities = 29/113 (25%), Positives = 51/113 (44%), Gaps = 7/113 (6%)
Query: 45 KWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELE----D 100
KW++ K+ ++ + RG ++ + + PKK D+I QEE+E + ++ D
Sbjct: 303 KWEKIKQHYQHWSDSLSEEGRG-LLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSD 361
Query: 101 LDKYQEKNTYKSLGFGGRD--REEEIGTLSEYTYRTDNKFGENDVETTREVAL 151
+EK K L RD EEE L+ + N E + E +++ L
Sbjct: 362 FLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKL 414
>YKZ6_YEAST (P36112) Hypothetical 61.1 kDa protein in YPT52-DBP7
intergenic region
Length = 540
Score = 33.9 bits (76), Expect = 0.22
Identities = 32/140 (22%), Positives = 62/140 (43%), Gaps = 25/140 (17%)
Query: 44 TKWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELEDLDK 103
T+++ K+EFE N +N+L V K +L Q+++E EEK+ E+L +
Sbjct: 214 TQYENSKREFEK-----------NLQKNLLQEVDEFKENLTKQKDKELEEKLKANEELLQ 262
Query: 104 YQEKNTYKSLGFGGRDREEEIGTLSEYTYRTDNKFGENDVETTREVALKFMVVIIVVMVI 163
+ N E+G LS + NK ++ +E R L + I +
Sbjct: 263 AKHAN--------------EVGLLSITQVKEFNKIIKDKIEKERNGRLAHLEEINSEVND 308
Query: 164 VNYLVDMSKTVMAQGQSYFQ 183
++ +D S ++++ ++ Q
Sbjct: 309 LSKSIDRSSKILSKNEALVQ 328
>PP71_HCMVA (P06726) 71 kDa upper matrix phosphoprotein (PP71)
Length = 559
Score = 33.1 bits (74), Expect = 0.37
Identities = 18/53 (33%), Positives = 25/53 (46%)
Query: 65 RGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELEDLDKYQEKNTYKSLGFGG 117
R N M+ +S LP+ L P EE+EEEE+ + +DL F G
Sbjct: 394 RSNVMRGAVSEFLPQSPGLPPTEEEEEEEEEDDEDDLSSTPTPTPLSEAMFAG 446
>MAD1_KLUDE (Q874L7) Spindle assembly checkpoint component MAD1
(Mitotic arrest deficient protein 1)
Length = 674
Score = 33.1 bits (74), Expect = 0.37
Identities = 27/126 (21%), Positives = 61/126 (47%), Gaps = 8/126 (6%)
Query: 45 KWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELEDLDKY 104
+W++ K EFE + ED +R + + +++ + DL+ + E+EE +I E D
Sbjct: 53 QWQKEKYEFEKRQLAAEDRLRN--ANDEMDILIQRNQDLVYRIEEEERNRINNDEGNDL- 109
Query: 105 QEKNTYKSLGFGGRDREEEIGTLSE---YTYRTDNKFGENDVETTREVALKFMVVIIVVM 161
K S+ G + EE+ E + ++T N+ + ++++ + + K+ +++
Sbjct: 110 --KGGAGSMDKGKKQEIEELSRRYEADRFEWKTQNEELDLELKSCKSLISKYEQLLMEQS 167
Query: 162 VIVNYL 167
+N L
Sbjct: 168 EKINSL 173
>HS11_LYCES (P30221) 17.8 kDa class I heat shock protein
Length = 154
Score = 32.0 bits (71), Expect = 0.82
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 19/87 (21%)
Query: 23 EQDRDTIELHLPGNGERPFDGTKWKR-------FKKEFELPKYCNEDAIRGNFMQNILSV 75
E+DR +++ N E+ KW R F + F LP+ D ++ + +L+V
Sbjct: 77 EEDR-VLQISGERNVEKEDKNDKWHRMERSSGKFMRRFRLPENAKMDQVKASMENGVLTV 135
Query: 76 VLPKKVDLIPQEEQEEEEKIPELEDLD 102
+PK EE K PE++ ++
Sbjct: 136 TVPK-----------EEVKKPEVKSIE 151
>SP21_STIAU (Q06823) Spore protein SP21
Length = 188
Score = 31.6 bits (70), Expect = 1.1
Identities = 14/56 (25%), Positives = 28/56 (50%)
Query: 49 FKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELEDLDKY 104
F + F LP+ + D +R + +L++ LPK+ ++ P+ Q + E + Y
Sbjct: 115 FSRAFTLPEGVDGDNVRADLKNGVLTLTLPKRPEVQPKRIQVASSGTEQKEHIKAY 170
>HS11_HELAN (P30693) 17.6 kDa class I heat shock protein
Length = 153
Score = 31.6 bits (70), Expect = 1.1
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 10/55 (18%)
Query: 48 RFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIPELEDLD 102
+F + F LP+ D ++ +L+VV+PK EEEEK P ++ +D
Sbjct: 106 KFIRRFRLPENAKMDEVKAMMENGVLTVVVPK----------EEEEKKPMVKAID 150
>VATI_PYRHO (O57721) V-type ATP synthase subunit I (EC 3.6.3.14)
(V-type ATPase subunit I)
Length = 659
Score = 31.2 bits (69), Expect = 1.4
Identities = 28/82 (34%), Positives = 34/82 (41%), Gaps = 19/82 (23%)
Query: 82 DLIPQEEQEEEEKIPELEDLDK---------YQEKNTYKSLGFGGRDREEEIGTLSEYTY 132
DLIP ++ +EK ELE++ Y E YK L D E E G Y
Sbjct: 234 DLIPIYTEKIKEKEKELEEVKSRGRKVAERYYDELVFYKEL----MDNEREKGNYLSYLV 289
Query: 133 RTDNKFG------ENDVETTRE 148
RT+ FG E DVE E
Sbjct: 290 RTEMTFGLLAWVPEKDVEKVVE 311
>TIG_AQUAE (O67358) Trigger factor (TF)
Length = 478
Score = 31.2 bits (69), Expect = 1.4
Identities = 17/48 (35%), Positives = 30/48 (62%)
Query: 47 KRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEK 94
K++ KE L + EDA R ++ I+S V K+V+ +EE++EE++
Sbjct: 421 KKYFKENNLEQVVYEDARRKKALKEIISKVKIKEVEQKQEEEKKEEKE 468
>SYRC_YEAST (Q05506) Arginyl-tRNA synthetase, cytoplasmic (EC
6.1.1.19) (Arginine--tRNA ligase) (ArgRS)
Length = 607
Score = 31.2 bits (69), Expect = 1.4
Identities = 31/103 (30%), Positives = 48/103 (46%), Gaps = 20/103 (19%)
Query: 23 EQDRDTIELHLPGNGE------RPFDGTK-----WKRFKKEFELPKYCNEDAIRGNFMQN 71
E++ D+I L NG+ R DG + WKRF+ EF + KY + A R N +
Sbjct: 232 EEEGDSIPLEQSTNGKAREYFKRMEDGDEEALKIWKRFR-EFSIEKYIDTYA-RLNIKYD 289
Query: 72 ILS-------VVLPKKVDLIPQEEQEEEEKIPELEDLDKYQEK 107
+ S + K +DL ++ E+K L DL K+ +K
Sbjct: 290 VYSGESQVSKESMLKAIDLFKEKGLTHEDKGAVLIDLTKFNKK 332
>HS13_ARATH (P19037) 18.2 kDa class I heat shock protein (HSP 18.2)
Length = 161
Score = 31.2 bits (69), Expect = 1.4
Identities = 16/70 (22%), Positives = 32/70 (44%), Gaps = 7/70 (10%)
Query: 24 QDRDTIELHLPGNGERPFDGTKWKR-------FKKEFELPKYCNEDAIRGNFMQNILSVV 76
+D++ +++ + E KW R F + F LP+ + ++ +L+VV
Sbjct: 82 EDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVV 141
Query: 77 LPKKVDLIPQ 86
+PK + PQ
Sbjct: 142 VPKAPEKKPQ 151
>SYA_CHLCV (Q821R2) Alanyl-tRNA synthetase (EC 6.1.1.7)
(Alanine--tRNA ligase) (AlaRS)
Length = 875
Score = 30.8 bits (68), Expect = 1.8
Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 9/85 (10%)
Query: 33 LPGNGERPFDGTKWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEE 92
LPGN ER + K R + R F Q L+ ++P VD + + E
Sbjct: 291 LPGNTERGYVLRKILRRAVNY---------GKRLGFTQPFLADIVPSLVDTMGEAYPELR 341
Query: 93 EKIPELEDLDKYQEKNTYKSLGFGG 117
+ +++++ +E+N +KSL GG
Sbjct: 342 LSLSQIQEVMTMEEENYFKSLHRGG 366
>SCPA_STRMU (Q7ZAK8) Segregation and condensation protein A
Length = 235
Score = 30.8 bits (68), Expect = 1.8
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Query: 64 IRGNFMQNILSVVLPKKVDLIPQE-EQEEEEKIPELEDLDKYQEKNTYKSLG 114
+ G +M ++L K L+P+ E E E+ PEL+ L + +E TYK LG
Sbjct: 52 VAGEYMVMASQLMLIKSRKLLPKVVETIEAEEDPELQLLHQIEEYRTYKLLG 103
>HOS4_YEAST (P40480) Protein HOS4
Length = 1083
Score = 30.8 bits (68), Expect = 1.8
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Query: 42 DGTKWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEQEEEEKIP 96
D +KRF LP Y D F+ ++ ++L K +DL+ ++ Q EKIP
Sbjct: 890 DKLDYKRF-----LPLYYFVDEKNDKFVLDLQVMILLKDIDLLSKDNQPTSEKIP 939
>GRPE_CLOTE (Q892Q9) GrpE protein (HSP-70 cofactor)
Length = 200
Score = 30.8 bits (68), Expect = 1.8
Identities = 28/105 (26%), Positives = 50/105 (46%), Gaps = 6/105 (5%)
Query: 80 KVDLIPQE-EQEEEEKIPELEDLDKYQEKNTYKSLGFGGRDREE----EIGTLSEYTYRT 134
++DL +E E+ E + E E+LDK +E+N + L +D E E+ L + RT
Sbjct: 17 EMDLNEEELEESEVNEDKEFEELDKSEEENEVEELISTLKDENEKLNNEMEALKDRLLRT 76
Query: 135 DNKFGENDVETTRE-VALKFMVVIIVVMVIVNYLVDMSKTVMAQG 178
++ T RE L V+ I+ L ++ + ++A+G
Sbjct: 77 TGEYDNYRKRTDREKEGLYASACEDVLKEILPVLDNLERAILAKG 121
>E2BD_YEAST (P12754) Translation initiation factor eIF-2B delta
subunit (eIF-2B GDP-GTP exchange factor) (Guanine
nucleotide exchange factor subunit GCD2) (GCD complex
subunit GCD2)
Length = 651
Score = 30.8 bits (68), Expect = 1.8
Identities = 22/89 (24%), Positives = 39/89 (43%), Gaps = 1/89 (1%)
Query: 36 NGERPFDGTKWKRFKKEFELPKYCNEDAIRG-NFMQNILSVVLPKKVDLIPQEEQEEEEK 94
NGE+ + K KK+ + K G + Q + K+ + +E+Q++ E+
Sbjct: 29 NGEKKLTNKELKELKKQEKAAKRAAMKQANGISIEQQQQQAQMKKEKKQLQREQQQKREQ 88
Query: 95 IPELEDLDKYQEKNTYKSLGFGGRDREEE 123
+ + K E+N KS FG + EE
Sbjct: 89 KQKNANKKKQNERNVKKSTLFGHLETTEE 117
>ARS2_MOUSE (Q99MR6) Arsenite-resistance protein 2
Length = 875
Score = 30.8 bits (68), Expect = 1.8
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 9/80 (11%)
Query: 82 DLIPQEEQEEEEKIPELEDLDKYQEKNTYKSLGFGGR-----DREEEIGTLSEYTYRTDN 136
DL E++EEEE+ + + K +E +LG G R D ++E G +E D+
Sbjct: 264 DLRILEQEEEEEQAGKTGEASKKEEARAGPALGEGERKANDKDEKKEDGKQAENDSSNDD 323
Query: 137 KF----GENDVETTREVALK 152
K G+ D E +E A K
Sbjct: 324 KTKKSEGDGDKEEKKEEAEK 343
>YAH3_YEAST (P39706) Hypothetical 48.7 kDa Trp-Asp repeats
containing protein in TFC3-RFA1 intergenic region
Length = 426
Score = 30.4 bits (67), Expect = 2.4
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Query: 72 ILSVVLPKKVDLIPQEEQEEEEKIPELEDLDKYQEKNTYKSLGFGGRDREEEIG----TL 127
+ SVV+P K + + +E EE + LE D++ E + + G ++EEEI T
Sbjct: 339 VWSVVIPPKWSALAPDFEEVEENVDYLEKEDEFDEVDEAEQQ--QGLEQEEEIAIDLRTR 396
Query: 128 SEYTYRTDN 136
+Y R +N
Sbjct: 397 EQYDVRGNN 405
>HS82_YEAST (P02829) ATP-dependent molecular chaperone HSP82 (Heat
shock protein Hsp90 heat inducible isoform) (82 kDa heat
shock protein)
Length = 709
Score = 30.4 bits (67), Expect = 2.4
Identities = 27/90 (30%), Positives = 47/90 (52%), Gaps = 21/90 (23%)
Query: 38 ERPFDGTKWKRFKKEFELPKYCNEDAIR------GNFMQNILSVVLPKKVDL---IPQEE 88
ER GT + F K+ +L +Y E I+ F+ + +V+ K+V+ IP+EE
Sbjct: 165 ERIGRGTILRLFLKDDQL-EYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEE 223
Query: 89 QEEEEKI-----------PELEDLDKYQEK 107
+++EEK P+LE++D+ +EK
Sbjct: 224 KKDEEKKDEEKKDEDDKKPKLEEVDEEEEK 253
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,830,660
Number of Sequences: 164201
Number of extensions: 1113994
Number of successful extensions: 4744
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 4671
Number of HSP's gapped (non-prelim): 100
length of query: 184
length of database: 59,974,054
effective HSP length: 104
effective length of query: 80
effective length of database: 42,897,150
effective search space: 3431772000
effective search space used: 3431772000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC126009.2