BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC124970.5 - phase: 0 
         (952 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

VGNB_CPSMV (P36312) Genome polyprotein B [Contains: Protease cof...    38  0.15
SLS4_BRAOL (P17841) S-locus-specific glycoprotein S14 precursor ...    34  1.6
HUL5_YEAST (P53119) Probable ubiquitin--protein ligase HUL5 (EC ...    34  1.6
YG4F_YEAST (P42938) Kinase YGR205W (EC 2.7.-.-)                        33  2.8
GYRB_RICPR (Q9ZCX2) DNA gyrase subunit B (EC 5.99.1.3)                 33  2.8

>VGNB_CPSMV (P36312) Genome polyprotein B [Contains: Protease
           cofactor; Membrane-binding protein; VPG; Protease (EC
           3.4.22.-); RNA-directed RNA polymerase (EC 2.7.7.48)]
          Length = 1858

 Score = 37.7 bits (86), Expect = 0.15
 Identities = 34/137 (24%), Positives = 56/137 (40%), Gaps = 29/137 (21%)

Query: 231 REMVLDGVKPDPVTVSCILSACSDLQDLKSGKAIHG-----------FALKHGMVENVFV 279
           REM++ G+K  P     I   C+DL  L +G  +HG           +A     V    V
Sbjct: 432 REMIMGGMKVSPTVCGLINKGCTDLAKLMAGAVMHGTSGTRKIPFVVYAHGASRVGKTMV 491

Query: 280 SNALVNLYESCLCVREAQAVFDLMPHRNVIT--WNSLASCYVNCGFPQKGLNVFREMGLN 337
            N L+  +      +E +   D +  RNV+   W+         G+ ++ + V  + G  
Sbjct: 492 INRLIEDFR-----KELELGEDCVYPRNVVDDYWS---------GYKRQPIVVIDDFG-- 535

Query: 338 GVKPDPMAMSSILPACS 354
            V  DP A + ++P  S
Sbjct: 536 AVSSDPSAEAQLIPLIS 552


>SLS4_BRAOL (P17841) S-locus-specific glycoprotein S14 precursor
           (SLSG-14) (Fragment)
          Length = 434

 Score = 34.3 bits (77), Expect = 1.6
 Identities = 27/109 (24%), Positives = 53/109 (47%), Gaps = 11/109 (10%)

Query: 544 DEITWSVVIGGCVKNSRIEEAMEIFRKMQTMGFKPDETTIYSILRACSLSEC-LRMGKEI 602
           D+  W+   GGC++ +R+  + + F +M+ M  K  ETT+  + R+  + EC  R   + 
Sbjct: 330 DQRVWA---GGCIRRTRLSGSGDGFTRMKNM--KLPETTMAIVDRSIGVKECEKRCLNDC 384

Query: 603 HCYVF-----RHWKDWDLARTNALVDMYAKCGGLSLSRNVFDMMPIKDV 646
           +C  F     R+     +  T  L DM +   G + S++++  +   D+
Sbjct: 385 NCTAFANADIRNGGTGCVINTGELEDMRSYATGATDSQDLYVRLAAADI 433


>HUL5_YEAST (P53119) Probable ubiquitin--protein ligase HUL5 (EC
           6.3.2.-)
          Length = 910

 Score = 34.3 bits (77), Expect = 1.6
 Identities = 30/124 (24%), Positives = 57/124 (45%), Gaps = 20/124 (16%)

Query: 771 SAKKLFEIDPNGSANYVTLFNILVTAKLWSEASKIRKLMK-----ERGITKTPGCSWFHV 825
           S+ K+ ++ PNGS  YVT  N+L+     ++    ++  K       G++      W  +
Sbjct: 723 SSAKVVDLIPNGSKTYVTKDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEM 782

Query: 826 GNRV--HTFVAGDKSNMESDKIYNFLDELFAKIKAAGYKPD----TDY--VLHDIDQEEK 877
            N +     ++G++ N++       LD+L +  +  GYK +     D+  VL++   EEK
Sbjct: 783 FNSIELQMLISGERDNID-------LDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEK 835

Query: 878 AESL 881
              L
Sbjct: 836 LNFL 839


>YG4F_YEAST (P42938) Kinase YGR205W (EC 2.7.-.-)
          Length = 290

 Score = 33.5 bits (75), Expect = 2.8
 Identities = 21/68 (30%), Positives = 30/68 (43%), Gaps = 5/68 (7%)

Query: 822 WFHVGNRVHTFV-----AGDKSNMESDKIYNFLDELFAKIKAAGYKPDTDYVLHDIDQEE 876
           WF  GN+   F+      G   +  S +IYN L E +   K+ GY    D+ L   DQ +
Sbjct: 23  WFETGNKCPLFIFFSGPQGSGKSFTSIQIYNHLMEKYGGEKSIGYASIDDFYLTHEDQLK 82

Query: 877 KAESLCNH 884
             E   N+
Sbjct: 83  LNEQFKNN 90


>GYRB_RICPR (Q9ZCX2) DNA gyrase subunit B (EC 5.99.1.3)
          Length = 807

 Score = 33.5 bits (75), Expect = 2.8
 Identities = 28/136 (20%), Positives = 58/136 (42%), Gaps = 12/136 (8%)

Query: 253 SDLQDLKSGKAIHGFALKHGMVENVFVSNALVNLYESCLCVREAQAVFDLMPHR------ 306
           ++LQ LK    I  F    G+ E   +    + L+E     + A ++FD+   R      
Sbjct: 654 TNLQVLKHENKIEFFHFSRGLKETKILLKEQLELFEFVEISQFALSIFDIFSKRLKLIVK 713

Query: 307 ----NVITWNSLASCYVNCGFPQKGLNVFREMGLNGVKPDPMAMSSILPACSQLKDLKSG 362
               +++T + L +  + CG  +KG+ + R  GL  +  D +  +++ P    L  ++  
Sbjct: 714 SKAFDILTPSQLLNTIIECG--KKGITIQRFKGLGEMNSDQLWETTLDPTKRTLLQVRVA 771

Query: 363 KTIHGFAVKHGMVEDV 378
           +      +   ++ DV
Sbjct: 772 EVDEAEGIFSTLMGDV 787


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.324    0.137    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 112,185,338
Number of Sequences: 164201
Number of extensions: 4658723
Number of successful extensions: 12862
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 12856
Number of HSP's gapped (non-prelim): 10
length of query: 952
length of database: 59,974,054
effective HSP length: 120
effective length of query: 832
effective length of database: 40,269,934
effective search space: 33504585088
effective search space used: 33504585088
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 71 (32.0 bits)