BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.9 - phase: 0
(122 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KOK0_YEAST (Q12236) Probable serine/threonine-protein kinase YOL... 32 0.25
COZ2_MOUSE (Q9JHH9) Coatomer zeta-2 subunit (Zeta-2 coat protein... 32 0.33
YJH6_YEAST (P47035) Hypothetical 128.5 kDa protein in SCP160-SMC... 31 0.56
SEC8_SCHPO (O74562) Exocyst complex component sec8 31 0.56
TIN2_HUMAN (Q9BSI4) TERF1-interacting nuclear factor 2 (TRF1-int... 30 1.3
MPP5_HUMAN (Q8N3R9) MAGUK p55 subfamily member 5 30 1.3
MAD1_CANGA (Q6FS63) Spindle assembly checkpoint component MAD1 (... 30 1.3
COZ2_HUMAN (Q9P299) Coatomer zeta-2 subunit (Zeta-2 coat protein... 29 1.6
TRA2_CAEEL (P34709) Sex-determining transformer protein 2 precur... 28 3.6
RMUC_NEIMB (Q9JXH2) DNA recombination protein rmuC homolog 28 3.6
NOG2_CRYBA (Q6TGJ8) Nucleolar GTP-binding protein 2 28 3.6
SSB_HELHP (P59931) Single-strand binding protein (SSB) (Helix-de... 28 4.8
MPP5_MOUSE (Q9JLB2) MAGUK p55 subfamily member 5 (Protein associ... 28 4.8
BIR3_MOUSE (O08863) Baculoviral IAP repeat-containing protein 3 ... 28 4.8
TS22_CHICK (Q91012) Regulatory protein TSC-22 (TGFB stimulated c... 27 6.2
TDP2_HUMAN (Q14188) Transcription factor Dp-2 (E2F dimerization ... 27 6.2
RLK5_ARATH (P47735) Receptor-like protein kinase 5 precursor (EC... 27 6.2
MATK_PISST (Q8WHM8) Maturase K (Intron maturase) 27 6.2
VG56_BPMD2 (O64247) Gene 56 protein (GP56) 27 8.1
TDP2_MOUSE (Q64163) Transcription factor Dp-2 (E2F dimerization ... 27 8.1
>KOK0_YEAST (Q12236) Probable serine/threonine-protein kinase
YOL100W (EC 2.7.1.37)
Length = 1081
Score = 32.0 bits (71), Expect = 0.25
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 5/59 (8%)
Query: 3 KGGKLMKLKSALKKWNSFGNGKQSRHSISAVADEDSSSSRSDLHTVFVGKSRRLYRVTS 61
+GG L +L + N G G + + S + D+D S S + +VF GK ++L+ TS
Sbjct: 795 EGGDLSELLT-----NRSGEGYKCNQNSSPMKDDDKSESNNKGSSVFSGKIKKLFHPTS 848
>COZ2_MOUSE (Q9JHH9) Coatomer zeta-2 subunit (Zeta-2 coat protein)
(Zeta-2 COP)
Length = 205
Score = 31.6 bits (70), Expect = 0.33
Identities = 26/88 (29%), Positives = 41/88 (46%), Gaps = 14/88 (15%)
Query: 38 SSSSRSDLHTVFVGKSRRLYRVTSDVVDNPVFRELVERSRETEQQNDTVNVVACEVVLFE 97
+ +SR++ F+G +Y+ + D+ F +V S E E ++V+AC
Sbjct: 78 NKTSRTESEIAFLGGMTIVYKSSIDI-----FLYVVGSSSENELM--LMSVLACLFDSLS 130
Query: 98 HLL-------WMLENADPQPESLDELVD 118
H+L W+LEN D LDE VD
Sbjct: 131 HILRKNVEKRWLLENMDGAFLVLDETVD 158
>YJH6_YEAST (P47035) Hypothetical 128.5 kDa protein in SCP160-SMC3
intergenic region
Length = 1189
Score = 30.8 bits (68), Expect = 0.56
Identities = 29/74 (39%), Positives = 37/74 (49%), Gaps = 9/74 (12%)
Query: 15 KKWNSFGNGKQSRHSISAVADE-DSSSSRSDLHTVFVGKSRRLYRVTSDVVDNPVFRELV 73
+K NS N S S S+ DE SSSS SD T K+RR+ VV+ P RE V
Sbjct: 971 EKNNSSANDDDSSSSGSSTEDESSSSSSSSDEETSTSRKARRV------VVNTP--REPV 1022
Query: 74 ERSRETEQQNDTVN 87
S + E + +VN
Sbjct: 1023 RSSSKIEAPSPSVN 1036
>SEC8_SCHPO (O74562) Exocyst complex component sec8
Length = 1088
Score = 30.8 bits (68), Expect = 0.56
Identities = 21/73 (28%), Positives = 34/73 (45%), Gaps = 1/73 (1%)
Query: 45 LHTVFVGKSRRLYRVTSDVVDNPVFRELVERSRETEQQNDTVNV-VACEVVLFEHLLWML 103
LHT+ V SD+ V +E + +TE +DTV V + + V + + L
Sbjct: 695 LHTLLVRFRNHCSDYLSDLCRTAVLKEYKHVNEDTEDVDDTVRVKLLHDDVTYPQFIKFL 754
Query: 104 ENADPQPESLDEL 116
+ +P E L+EL
Sbjct: 755 KQKNPSLEGLNEL 767
>TIN2_HUMAN (Q9BSI4) TERF1-interacting nuclear factor 2
(TRF1-interacting nuclear protein 2)
Length = 451
Score = 29.6 bits (65), Expect = 1.3
Identities = 21/76 (27%), Positives = 38/76 (49%), Gaps = 4/76 (5%)
Query: 37 DSSSSRSDLHTVFVGKSRRLYRVTSDVVDNPVFRELVERSRETEQQNDTVNVVACEVVLF 96
D +++ DL + + Y+ + + PV +L + +E EQ+ + A E +LF
Sbjct: 96 DPKATKQDLRKILEAQET-FYQQVKQLSEAPV--DLASKLQELEQEYGEPFLAAMEKLLF 152
Query: 97 EHLLWMLENADPQPES 112
E+L LE A P P++
Sbjct: 153 EYLC-QLEKALPTPQA 167
>MPP5_HUMAN (Q8N3R9) MAGUK p55 subfamily member 5
Length = 675
Score = 29.6 bits (65), Expect = 1.3
Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 11/80 (13%)
Query: 43 SDL--HTVFVG--KSRRLYRVTSDVVDNPV---FRELVERSRETEQQN----DTVNVVAC 91
SDL + +F+ RL + + V NP RE++E++RE EQ N DT V +
Sbjct: 588 SDLKPYIIFIAPPSQERLRALLAKEVKNPKPEELREIIEKTREMEQSNGHYFDTAIVNSD 647
Query: 92 EVVLFEHLLWMLENADPQPE 111
++ LL ++ D +P+
Sbjct: 648 LDKAYQELLRLINKLDTEPQ 667
>MAD1_CANGA (Q6FS63) Spindle assembly checkpoint component MAD1
(Mitotic arrest deficient protein 1)
Length = 657
Score = 29.6 bits (65), Expect = 1.3
Identities = 30/128 (23%), Positives = 62/128 (48%), Gaps = 20/128 (15%)
Query: 6 KLMKLKSALKKWNSF-GNGKQSRHSISAVADE---DSSSSRSDLHTVFVGKSRRLYRVTS 61
++M+LK+ L++WN F N KQ +S D + S R +L+ V + L V
Sbjct: 257 EIMELKANLEEWNKFLSNKKQQDNSNDYSIDHFIIEFESQRRELNMV----TEELAEVKK 312
Query: 62 DVVDNPVFRE--LVERSR----ETEQQNDTVNV------VACEVVLFEHLLWMLENADPQ 109
++ + + +ER++ + + +N+ +N+ + + VL E +L+N P
Sbjct: 313 SYYNSKILNDELALERNQLLKLKDDYENNIINLEKLNHELEQQKVLLEEECSLLKNQIPT 372
Query: 110 PESLDELV 117
E+L++ +
Sbjct: 373 KENLNQQI 380
>COZ2_HUMAN (Q9P299) Coatomer zeta-2 subunit (Zeta-2 coat protein)
(Zeta-2 COP)
Length = 210
Score = 29.3 bits (64), Expect = 1.6
Identities = 25/88 (28%), Positives = 40/88 (45%), Gaps = 14/88 (15%)
Query: 38 SSSSRSDLHTVFVGKSRRLYRVTSDVVDNPVFRELVERSRETEQQNDTVNVVACEVVLFE 97
+ +SR++ F G +Y+ + D+ F +V S E E ++V+ C
Sbjct: 83 NKTSRTESEIAFFGGMTIVYKNSIDL-----FLYVVGSSYENELM--LMSVLTCLFESLN 135
Query: 98 HLL-------WMLENADPQPESLDELVD 118
H+L W+LEN D LDE+VD
Sbjct: 136 HMLRKNVEKRWLLENMDGAFLVLDEIVD 163
>TRA2_CAEEL (P34709) Sex-determining transformer protein 2 precursor
(Ce-Tra-2)
Length = 1475
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Query: 55 RLYRVTSDVVDNPVFRELVERSRETEQQNDTVNVVACEVVLFEHLLW 101
++ RVTS V+DN V +L S E + + VNV + V+LF +W
Sbjct: 421 KMIRVTSRVLDNTVTTKLQSFS-EKQTIHFVVNVHSLIVILFTIFVW 466
>RMUC_NEIMB (Q9JXH2) DNA recombination protein rmuC homolog
Length = 594
Score = 28.1 bits (61), Expect = 3.6
Identities = 25/89 (28%), Positives = 41/89 (45%), Gaps = 6/89 (6%)
Query: 3 KGGKLM-KLKSALKKWNSFGNGKQSRHSISAVADEDSSSSRSDLHTVFVGKSRRLYRVTS 61
+GGKL K ++ S G G S A + + R +L VG++ +L R+
Sbjct: 506 EGGKLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNL----VGRAEKL-RLLG 560
Query: 62 DVVDNPVFRELVERSRETEQQNDTVNVVA 90
+ R+LVERS ET ++++ A
Sbjct: 561 VKAGKQLQRDLVERSNETTALSESLEYAA 589
>NOG2_CRYBA (Q6TGJ8) Nucleolar GTP-binding protein 2
Length = 731
Score = 28.1 bits (61), Expect = 3.6
Identities = 14/38 (36%), Positives = 21/38 (54%), Gaps = 2/38 (5%)
Query: 47 TVFVGKSRRLYRVTSDVVDNPVFRELVERSRETEQQND 84
T F+G R Y +VVD +E+VE+ E E++ D
Sbjct: 572 TKFIGDDARRYVEEEEVVDQD--KEMVEKEEEEEEEED 607
>SSB_HELHP (P59931) Single-strand binding protein (SSB)
(Helix-destabilizing protein)
Length = 170
Score = 27.7 bits (60), Expect = 4.8
Identities = 13/33 (39%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Query: 1 MAKGGK-LMKLKSALKKWNSFGNGKQSRHSISA 32
+ KG K L++ + +L+ WN K+SRH+I+A
Sbjct: 67 LRKGSKILIEGRLSLESWNDQSGAKRSRHTITA 99
>MPP5_MOUSE (Q9JLB2) MAGUK p55 subfamily member 5 (Protein
associated with Lin-7 1)
Length = 675
Score = 27.7 bits (60), Expect = 4.8
Identities = 15/46 (32%), Positives = 26/46 (55%), Gaps = 4/46 (8%)
Query: 70 RELVERSRETEQQN----DTVNVVACEVVLFEHLLWMLENADPQPE 111
RE++E++RE EQ N DT V + ++ LL ++ D +P+
Sbjct: 622 REIIEKTREMEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQ 667
>BIR3_MOUSE (O08863) Baculoviral IAP repeat-containing protein 3
(Inhibitor of apoptosis protein 1) (MIAP1) (MIAP-1)
Length = 600
Score = 27.7 bits (60), Expect = 4.8
Identities = 15/49 (30%), Positives = 28/49 (56%), Gaps = 6/49 (12%)
Query: 57 YRVTSDVV------DNPVFRELVERSRETEQQNDTVNVVACEVVLFEHL 99
YR SD+V ++ + E +E++ E E+ +D + ++VLF+HL
Sbjct: 407 YRTVSDLVIGLLDAEDEMREEQMEQAAEEEESDDLALIRKNKMVLFQHL 455
>TS22_CHICK (Q91012) Regulatory protein TSC-22 (TGFB stimulated
clone 22 homolog)
Length = 144
Score = 27.3 bits (59), Expect = 6.2
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 6/66 (9%)
Query: 26 SRHSISAVADEDSSSSRSDLHTVFVGKSRRLYRVTSDV-VDNPVFRELVERSRETEQQND 84
S +S VA E+ DL KS +Y V +V V +EL+E++ + EQ+N
Sbjct: 42 SSSGVSVVAIENMIEQAMDLV-----KSHLMYAVREEVEVLKEQIKELIEKNSQLEQENT 96
Query: 85 TVNVVA 90
+ +A
Sbjct: 97 LLKTLA 102
>TDP2_HUMAN (Q14188) Transcription factor Dp-2 (E2F dimerization
partner 2)
Length = 446
Score = 27.3 bits (59), Expect = 6.2
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 7/52 (13%)
Query: 39 SSSRSDLHTVFVGKSRRLYRVTSD-------VVDNPVFRELVERSRETEQQN 83
++S + + + K RR+ R+ ++ F+ LV+R+R+ EQQN
Sbjct: 211 TNSAQECQNLEIEKQRRIERIKQKRAQLQELLLQQIAFKNLVQRNRQNEQQN 262
>RLK5_ARATH (P47735) Receptor-like protein kinase 5 precursor (EC
2.7.1.37)
Length = 999
Score = 27.3 bits (59), Expect = 6.2
Identities = 31/101 (30%), Positives = 45/101 (43%), Gaps = 17/101 (16%)
Query: 1 MAKGGKLMKLKS---ALKKWNSFGNGKQSRHSISAVADEDSSSSRSDLHTVFVGKSRRLY 57
+AK KL LKS A KW SF S H I+ DE + + G S ++Y
Sbjct: 647 IAKCRKLRALKSSTLAASKWRSFHKLHFSEHEIADCLDEK--------NVIGFGSSGKVY 698
Query: 58 RVT---SDVVDNPVFRELVERSRETEQQNDTVN--VVACEV 93
+V +VV + V + + E +D++N V A EV
Sbjct: 699 KVELRGGEVVAVKKLNKSV-KGGDDEYSSDSLNRDVFAAEV 738
>MATK_PISST (Q8WHM8) Maturase K (Intron maturase)
Length = 511
Score = 27.3 bits (59), Expect = 6.2
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 11/85 (12%)
Query: 49 FVGKSRRLY-RVTSD----VVDNPVFRELVERSRETEQQNDTVNVVACEVVL------FE 97
FVG ++ Y ++ S+ +V+ P LV E ++ + N+ + + F
Sbjct: 87 FVGHNKNFYSQMISEGFAVIVEIPFSLRLVSSLEEKKEIPKSQNLRSIHSIFPFFEDNFS 146
Query: 98 HLLWMLENADPQPESLDELVDFYAC 122
HL W+ + P P+ L+ LV C
Sbjct: 147 HLNWISDILIPYPDHLEMLVQILQC 171
>VG56_BPMD2 (O64247) Gene 56 protein (GP56)
Length = 90
Score = 26.9 bits (58), Expect = 8.1
Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 6/70 (8%)
Query: 54 RRLYRVTSDVVDNPVFRELVERSRETEQQN---DTVNVVACEVVLFEHLLWMLENADPQP 110
R +R+ + D V ++V+ +R E + D + + V++ +H ++ QP
Sbjct: 17 RPCHRIQQFLDDAGVEYDVVDLTRNAEAKTYVQDVLKASSVPVIVTDHFEPIIGY---QP 73
Query: 111 ESLDELVDFY 120
+ +DEL+D+Y
Sbjct: 74 DKVDELIDYY 83
>TDP2_MOUSE (Q64163) Transcription factor Dp-2 (E2F dimerization
partner 2) (Dp-3)
Length = 446
Score = 26.9 bits (58), Expect = 8.1
Identities = 14/51 (27%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Query: 40 SSRSDLHTVFVGKSRRLYRVTSD-------VVDNPVFRELVERSRETEQQN 83
+S + + + K RR+ R+ ++ F+ LV+R+R+ EQQN
Sbjct: 212 NSAQECQNLEIEKQRRIERIKQKRAQLQELLLQQIAFKNLVQRNRQNEQQN 262
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.130 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,312,286
Number of Sequences: 164201
Number of extensions: 495291
Number of successful extensions: 1337
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 1334
Number of HSP's gapped (non-prelim): 22
length of query: 122
length of database: 59,974,054
effective HSP length: 98
effective length of query: 24
effective length of database: 43,882,356
effective search space: 1053176544
effective search space used: 1053176544
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124967.9