BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.5 + phase: 0 /pseudo
(233 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
IF3B_ORYSA (Q94HF1) Eukaryotic translation initiation factor 3 s... 308 9e-84
IF3B_ARATH (Q9SZA3) Eukaryotic translation initiation factor 3 s... 298 6e-81
IF3B_DROME (Q9W2D9) Probable eukaryotic translation initiation f... 111 2e-24
IF3B_MOUSE (Q9DBZ5) Eukaryotic translation initiation factor 3 s... 105 9e-23
IF3B_HUMAN (Q9UBQ5) Eukaryotic translation initiation factor 3 s... 105 9e-23
IF3B_CAEEL (Q9XUP3) Probable eukaryotic translation initiation f... 75 1e-13
ACE_MOUSE (P09470) Angiotensin-converting enzyme, somatic isofor... 31 2.2
SODM_CHLMU (Q9PKA0) Superoxide dismutase [Mn] (EC 1.15.1.1) 29 8.3
>IF3B_ORYSA (Q94HF1) Eukaryotic translation initiation factor 3
subunit 11 (eIF-3 p25) (eIF3k)
Length = 226
Score = 308 bits (788), Expect = 9e-84
Identities = 158/222 (71%), Positives = 183/222 (82%), Gaps = 2/222 (0%)
Query: 13 QGGTAYTVEQLVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKMSSQ 72
Q +YTVE+LVA N YNPDIL DLE +VNDQVS+QTY+L++NL LLRLYQFE E++S Q
Sbjct: 5 QAAESYTVEELVAVNPYNPDILNDLEGFVNDQVSNQTYNLDANLSLLRLYQFEPERLSVQ 64
Query: 73 IVARILVKALMAMPAPDFSLCLFLIPERVIANGRSSSRH*L-FCLTI*RFRQFWDEASKS 131
IV+RIL+KALMAMP PDFSLCLFLIPE V + + L L RFRQFWDEASK+
Sbjct: 65 IVSRILIKALMAMPGPDFSLCLFLIPEHVQMEEQFKTLIVLSHYLETARFRQFWDEASKN 124
Query: 132 RHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENSGWVI 191
R+I++ VPGFEQAIQ YAIHVLSLTYQK+PR VLAEAIN+EGL+LDKFLEH + NSGWVI
Sbjct: 125 RNILDVVPGFEQAIQSYAIHVLSLTYQKVPRPVLAEAINIEGLALDKFLEHHIANSGWVI 184
Query: 192 EKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHVTRIFPIL 233
EK + QLIVLPRNEFN P LKKNTA++VP EHVTRIFP+L
Sbjct: 185 EKG-ARSQLIVLPRNEFNHPELKKNTAETVPFEHVTRIFPVL 225
>IF3B_ARATH (Q9SZA3) Eukaryotic translation initiation factor 3
subunit 11 (eIF-3 p25) (eIF3k)
Length = 226
Score = 298 bits (764), Expect = 6e-81
Identities = 155/222 (69%), Positives = 182/222 (81%), Gaps = 6/222 (2%)
Query: 13 QGGTAYTVEQLVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKMSSQ 72
Q ++YTVEQLVA N +NP+ILPDLENYVN V+SQTYSLE NLCLLRLYQFE E+M++
Sbjct: 9 QEQSSYTVEQLVALNPFNPEILPDLENYVN--VTSQTYSLEVNLCLLRLYQFEPERMNTH 66
Query: 73 IVARILVKALMAMPAPDFSLCLFLIPERVIANGRSSSRH*L-FCLTI*RFRQFWDEASKS 131
IVARILVKALMAMP PDFSLCLFLIPERV + S L L RF+QFWDEA+K+
Sbjct: 67 IVARILVKALMAMPTPDFSLCLFLIPERVQMEEQFKSLIVLSHYLETGRFQQFWDEAAKN 126
Query: 132 RHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENSGWVI 191
RHI+EAVPGFEQAIQ YA H+LSL+YQK+PR+VLAEA+N++G SLDKF+E QV NSGW++
Sbjct: 127 RHILEAVPGFEQAIQAYASHLLSLSYQKVPRSVLAEAVNMDGASLDKFIEQQVTNSGWIV 186
Query: 192 EKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHVTRIFPIL 233
EK G IVLP+NEFN P LKKNT ++VPLEH+ RIFPIL
Sbjct: 187 EKEGGS---IVLPQNEFNHPELKKNTGENVPLEHIARIFPIL 225
>IF3B_DROME (Q9W2D9) Probable eukaryotic translation initiation
factor 3 subunit 11 (eIF-3 p25) (eIF3k)
Length = 222
Score = 111 bits (277), Expect = 2e-24
Identities = 71/219 (32%), Positives = 119/219 (53%), Gaps = 13/219 (5%)
Query: 11 KGQGGTAYTVEQLVA-FNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKM 69
K + G + T+++++ RYNPD L LE+YV DQ + TY LE+NL +L+LYQF +
Sbjct: 6 KMENGQSQTIQEMLGCIERYNPDHLKTLESYVQDQAKNNTYDLEANLAVLKLYQFNPHML 65
Query: 70 SSQIVARILVKALMAMPAPDFSL--CLFLIPERVIANGRSSSRH*LFCLTI*RFRQFWDE 127
+ I IL+K+L ++P DF + CL L+P+++ + L F FW
Sbjct: 66 NFDITYTILLKSLTSLPHTDFVMAKCL-LLPQQMKDENVQTIIDLADILERADFTLFWQR 124
Query: 128 ASKSRHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENS 187
A +R++ + GF +I+ + HV+ T+Q I + +L E + G D LE ++ +
Sbjct: 125 AEVNRNMFRHITGFHDSIRKFVSHVVGTTFQTIRKDLLKELL---GGIEDSTLESWIKRN 181
Query: 188 GWVIEKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHV 226
GW K+QG+G +IV + +D I KN + + ++V
Sbjct: 182 GW---KNQGQGLVIVAMQ---DDKIKTKNITEKIEFDNV 214
>IF3B_MOUSE (Q9DBZ5) Eukaryotic translation initiation factor 3
subunit 11 (eIF-3 p25) (eIF3k)
Length = 218
Score = 105 bits (262), Expect = 9e-23
Identities = 67/210 (31%), Positives = 106/210 (49%), Gaps = 14/210 (6%)
Query: 23 LVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKMSSQIVARILVKAL 82
L +RYNP+ L LE YV Q Y LE+NL +L+LYQF + + A+IL+KAL
Sbjct: 15 LKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQILLKAL 74
Query: 83 MAMPAPDFSLCLFLIPERVIANGRSSSRH*LF---CLTI*RFRQFWDEASKSRHIVEAVP 139
+P DF+LC +I + R L+ L F+ FW ++ ++E +
Sbjct: 75 TNLPHTDFTLCKCMIDQ--AHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGIT 132
Query: 140 GFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQ 199
GFE +++ + HV+ +TYQ I R +LAE + G D L+ + GW ++S GQ
Sbjct: 133 GFEDSVRKFICHVVGITYQHIDRWLLAEML---GDLTDNQLKVWMSKYGWSADES---GQ 186
Query: 200 LIVLPRNEFNDPILKKNTADSVPLEHVTRI 229
+ + + E P KN + + + V+ I
Sbjct: 187 VFICSQEESIKP---KNIVEKIDFDSVSSI 213
>IF3B_HUMAN (Q9UBQ5) Eukaryotic translation initiation factor 3
subunit 11 (eIF-3 p25) (eIF3k) (Muscle specific gene M9
protein) (PTD001) (HSPC029) (ARG134)
Length = 218
Score = 105 bits (262), Expect = 9e-23
Identities = 67/210 (31%), Positives = 106/210 (49%), Gaps = 14/210 (6%)
Query: 23 LVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKMSSQIVARILVKAL 82
L +RYNP+ L LE YV Q Y LE+NL +L+LYQF + + A+IL+KAL
Sbjct: 15 LKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQILLKAL 74
Query: 83 MAMPAPDFSLCLFLIPERVIANGRSSSRH*LF---CLTI*RFRQFWDEASKSRHIVEAVP 139
+P DF+LC +I + R L+ L F+ FW ++ ++E +
Sbjct: 75 TNLPHTDFTLCKCMIDQ--AHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGIT 132
Query: 140 GFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQ 199
GFE +++ + HV+ +TYQ I R +LAE + G D L+ + GW ++S GQ
Sbjct: 133 GFEDSVRKFICHVVGITYQHIDRWLLAEML---GDLSDSQLKVWMSKYGWSADES---GQ 186
Query: 200 LIVLPRNEFNDPILKKNTADSVPLEHVTRI 229
+ + + E P KN + + + V+ I
Sbjct: 187 IFICSQEESIKP---KNIVEKIDFDSVSSI 213
>IF3B_CAEEL (Q9XUP3) Probable eukaryotic translation initiation
factor 3 subunit 11 (eIF-3 p25) (eIF3k)
Length = 240
Score = 75.1 bits (183), Expect = 1e-13
Identities = 58/224 (25%), Positives = 101/224 (44%), Gaps = 26/224 (11%)
Query: 21 EQLVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQFESEKMSSQIVARILVK 80
E + NRYNP+ + DL V V+ Y + L +L+LYQ EK +V ++L+K
Sbjct: 12 EAIEGVNRYNPENVADLAACVQAMVNENKYDKDIVLTILKLYQLNPEKYDEAVVRQVLLK 71
Query: 81 ALMAMPAPDFSLCLFLIPERVIANGRSSSRH*L-FCLTI*RFRQFW-------------- 125
LM +P+ DF+L LI + + L L F FW
Sbjct: 72 TLMVLPSSDFALAKCLIDTNRLGSQELRRIFDLGAVLESCNFAVFWKLVKGAYKPTTNPN 131
Query: 126 ---DEASKSRHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEH 182
+ +++ + GFE A++ YA V+S+T+QKI + +L+ + G + DK +
Sbjct: 132 EPFKVPGEVPKMIKPMVGFEDAVKHYACRVISVTFQKIEKKMLSRLL---GGASDKEVTA 188
Query: 183 QVENSGWVIEKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHV 226
++ GW +++ G + + +E I +N + + HV
Sbjct: 189 LAQSFGW---EAKENGDVFFVANHE--GTIKTRNIDEKIQFPHV 227
>ACE_MOUSE (P09470) Angiotensin-converting enzyme, somatic isoform
precursor (EC 3.4.15.1) (ACE) (Dipeptidyl
carboxypeptidase I) (Kininase II)
Length = 1312
Score = 31.2 bits (69), Expect = 2.2
Identities = 17/61 (27%), Positives = 31/61 (49%), Gaps = 1/61 (1%)
Query: 164 VLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQLIVLPRNEFNDPILKKNTADSVPL 223
VL + + + L LE+ S W+ E++Q G+++ P N++ P L N + + L
Sbjct: 589 VLKDLVGSDALDAKALLEYFQPVSQWLEEQNQRNGEVLGWPENQWRPP-LPDNYPEGIDL 647
Query: 224 E 224
E
Sbjct: 648 E 648
>SODM_CHLMU (Q9PKA0) Superoxide dismutase [Mn] (EC 1.15.1.1)
Length = 205
Score = 29.3 bits (64), Expect = 8.3
Identities = 22/73 (30%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
Query: 161 PRTVLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQLIVLPRNEFNDPILKKNTADS 220
PR L + I S D FL++ + +S V + G G L P+ + ++ + TA+
Sbjct: 96 PRHELLKLIEKFWGSFDNFLKNFISSSAAV--QGSGWGWLAFCPKKQ---ELMIQTTANQ 150
Query: 221 VPLEHVTRIFPIL 233
PLE T + P+L
Sbjct: 151 DPLEATTGMIPLL 163
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.139 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,450,570
Number of Sequences: 164201
Number of extensions: 946841
Number of successful extensions: 3017
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3001
Number of HSP's gapped (non-prelim): 9
length of query: 233
length of database: 59,974,054
effective HSP length: 107
effective length of query: 126
effective length of database: 42,404,547
effective search space: 5342972922
effective search space used: 5342972922
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC124967.5