BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC123570.3 + phase: 0 /pseudo
         (1023 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

MT18_YEAST (P40469) DNA repair/transcription protein MET18/MMS19       67  3e-10
YHI2_YEAST (P38763) Hypothetical 29.0 kDa protein in DED81-MYO1 ...    36  0.60
Y255_MYCGE (P47497) Hypothetical protein MG255                         32  6.7
MATK_LOTJA (Q9BBU2) Maturase K (Intron maturase)                       32  6.7
DUHM_BPSP1 (P31654) Deoxyuridylate hydroxymethyltransferase (EC ...    32  8.7

>MT18_YEAST (P40469) DNA repair/transcription protein MET18/MMS19
          Length = 1032

 Score = 66.6 bits (161), Expect = 3e-10
 Identities = 49/177 (27%), Positives = 81/177 (45%), Gaps = 6/177 (3%)

Query: 16  QHLHFQSLGLYDRKLCFELLDCLLEHHA--DSVASLEEDLIFGFCAAIDAERDPECLMPA 73
           Q  H  S  L+  K+  ++ D    + +  + V  + +  I  F    + E+DP  L+ +
Sbjct: 136 QGQHLASTRLWPFKILRKIFDRFFVNGSSTEQVKRINDLFIETFLHVANGEKDPRNLLLS 195

Query: 74  FHIVESLARLYPDPSGLFASFARDVFDLLEPYFPIQFTHTTSGDAHVHRDDLSRTLMNAF 133
           F + +S+     +      +F  D+FD+L  YFPI F         +   DL   L +A 
Sbjct: 196 FALNKSITSSLQNVE----NFKEDLFDVLFCYFPITFKPPKHDPYKISNQDLKTALRSAI 251

Query: 134 SSTPLFEPFVIPLLLQKLSSSLHSAKIDSLQYLRVCSSKYGAERIAKYVGAIWSSLK 190
           ++TPLF       LL KL++S    K D+L  L  C  K+G   I +    +W++LK
Sbjct: 252 TATPLFAEDAYSNLLDKLTASSPVVKNDTLLTLLECVRKFGGSSILENWTLLWNALK 308



 Score = 38.9 bits (89), Expect = 0.071
 Identities = 54/262 (20%), Positives = 101/262 (37%), Gaps = 48/262 (18%)

Query: 766  WIGKGLLLRGHEKIKDITKILTECLISDRNSSLPLIEGLDENNEEHKGDHLARKCAADAF 825
            W+ KGL+++   +  +I K   + L ++   SL                       +  F
Sbjct: 797  WLTKGLIMQNSLESSEIAKKFIDLLSNEEIGSL----------------------VSKLF 834

Query: 826  HVLMSDAEDCLNRK---FHATMRPLYKQRFFSSMMPIFLQLISRSDSSSSRYLLLRAFAR 882
             V + D       K   ++  ++ LYKQ+FF  +    +     +   + +   L A + 
Sbjct: 835  EVFVMDISSLKKFKGISWNNNVKILYKQKFFGDIFQTLVSNYKNTVDMTIKCNYLTALSL 894

Query: 883  VMSVTPLIVILNDAKELISVLLDCLSMLTEDIQDKDILYGLLLVLSGMLTEKNESL---- 938
            V+  TP   +     +L  +LL  L     D+ D ++    L  L    T+K+ +L    
Sbjct: 895  VLKHTPSQSVGPFINDLFPLLLQAL-----DMPDPEVRVSALETLKD-TTDKHHTLITEH 948

Query: 939  ------LFKAIQIKNDVNG*FLFLPILQLVRESCIQCLVALSKLPHVRIYPLRTQVLEAI 992
                  L  ++ + +  N   + L  LQL     ++ +  +  L +   Y  +  VL A+
Sbjct: 949  VSTIVPLLLSLSLPHKYNSVSVRLIALQL-----LEMITTVVPLNYCLSY--QDDVLSAL 1001

Query: 993  SKCLDDTKRSVRNEAVKCRQAW 1014
               L D KR +R + V  RQ +
Sbjct: 1002 IPVLSDKKRIIRKQCVDTRQVY 1023


>YHI2_YEAST (P38763) Hypothetical 29.0 kDa protein in DED81-MYO1
           intergenic region
          Length = 256

 Score = 35.8 bits (81), Expect = 0.60
 Identities = 39/162 (24%), Positives = 68/162 (41%), Gaps = 12/162 (7%)

Query: 179 AKYVGAIWSSLKDTINTYLMEPNFSFTLAPTDGID---FPKNEVVIEALSLLQQLIVQNS 235
           AK  G I+ S  + +   L+  N S T      ID   F  N++ +  LS L  L ++ S
Sbjct: 91  AKNSGTIYESCGNFLEERLINANKS-TAQRRTSIDVQVFDTNQMEVSYLSELTTLQIRQS 149

Query: 236 SQLVSLIIDDKDVNFIINSIASYEMYDAVSVQEKKKLHVIGRILYIFAKTSIPSCNAVFQ 295
             ++       D     +S+AS E Y  +    + +  +   I+    K  + S   +  
Sbjct: 150 DAIILCFDSTND-----SSLASLESYICIIHHVRLECELDIPIIIACTKCDLDSERTITH 204

Query: 296 SLLLRMMDSLGFSVSNIDGLKNAGILASQSVNFGFLYLCIEL 337
             +L  +  LGFS  N+D  + +   +  +VN   L+L + L
Sbjct: 205 EKVLTFIQELGFSPGNLDYFETS---SKFNVNVEDLFLAVLL 243


>Y255_MYCGE (P47497) Hypothetical protein MG255
          Length = 365

 Score = 32.3 bits (72), Expect = 6.7
 Identities = 37/132 (28%), Positives = 58/132 (43%), Gaps = 27/132 (20%)

Query: 162 SLQYLRVCSSKYGAERIAKYVG--AIWSSLKD---------TINTYLMEPNFSFTLAPTD 210
           +L +LR C  K    +I+K+V    I S +K          T+ T L  P FS T+A  D
Sbjct: 116 ALLWLRQCWLKLQLTKISKFVNQKGILSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQD 175

Query: 211 GIDFPKNEVVIEALSLLQQLIVQNSSQLVSLIIDDKDVNFIINSIASYEMYDAVSVQEKK 270
           GI       V   L +    +   S  LVS +I      F+++S+         S+++KK
Sbjct: 176 GIKQNAGNDVFIFLIIFS--VFSISLGLVSSLI------FLVSSL--------FSIRQKK 219

Query: 271 KLHVIGRILYIF 282
            L  + ++L  F
Sbjct: 220 TLDALDKVLSKF 231


>MATK_LOTJA (Q9BBU2) Maturase K (Intron maturase)
          Length = 508

 Score = 32.3 bits (72), Expect = 6.7
 Identities = 21/82 (25%), Positives = 38/82 (45%), Gaps = 5/82 (6%)

Query: 409 KSTFENILKKFMSIIIEDFNKTILWNSTLKSLFHIGSLFQNFSESEKAMSYRSFVLDKTM 468
           K+ +  I+ +  +II+E     IL++  L S      + +++       S   F  DK  
Sbjct: 87  KNFYSQIISEGFAIIVE-----ILFSLQLSSSLEEAEIIKSYKNLRSIHSIFPFFEDKVT 141

Query: 469 ELLSLDDISLPFSLKLEVLSDI 490
            L  + DI +P+ + LE+L  I
Sbjct: 142 YLNYISDIRVPYPIHLEILVQI 163


>DUHM_BPSP1 (P31654) Deoxyuridylate hydroxymethyltransferase (EC
           2.1.2.-) (Deoxyuridylate hydroxymethylase) (dUMP
           hydroxymethylase) (dUMP-HMase)
          Length = 383

 Score = 32.0 bits (71), Expect = 8.7
 Identities = 35/169 (20%), Positives = 68/169 (39%), Gaps = 20/169 (11%)

Query: 32  FELLDCLLEHHADSVASLEEDLIFGFCAAIDAERDPECLMPAFHIVESLARLYPDPSGLF 91
           +++ D L  +  D  A + ED+   +   ++ +  P        +VE    L  DP+   
Sbjct: 217 YQITDSLHVYLDDYGAKITEDIQKVYGVNLETDEAP--------VVEDFEELL-DPNPRM 267

Query: 92  ASFARDVFDLLEPYFPIQFTHTTSGDAHVHRDDLSRTLMNAFSSTPLFEPFVIPLLL--- 148
           +    ++   L  YF I  +     + ++H  D ++ L+N     P  E   + LLL   
Sbjct: 268 SYTMNELNQFLNEYFGIVDSLMRDDETYMHDGDCAQMLLNQIRHEP-DEYIKVTLLLMVA 326

Query: 149 -QKLSSSLHSAKIDSLQYLRVCSSKYGAERIAKYVGAIWSSLKDTINTY 196
            Q L+  +      ++ ++ +C  K  A R       ++ S  + IN Y
Sbjct: 327 KQALNRGMKDTVATAMNWIPLCEIKVSALRF------LYKSYPEYINQY 369


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.326    0.141    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,444,429
Number of Sequences: 164201
Number of extensions: 4467285
Number of successful extensions: 13785
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 13779
Number of HSP's gapped (non-prelim): 9
length of query: 1023
length of database: 59,974,054
effective HSP length: 120
effective length of query: 903
effective length of database: 40,269,934
effective search space: 36363750402
effective search space used: 36363750402
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 71 (32.0 bits)