BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122729.1 - phase: 0 /pseudo
(119 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 38 0.005
SCG2_XENLA (O13263) Amiloride-sensitive sodium channel gamma-2-s... 31 0.44
ER29_MOUSE (P57759) Endoplasmic reticulum protein ERp29 precursor 28 2.8
MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Act... 28 3.7
MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, iso... 28 3.7
MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, iso... 28 3.7
ER29_HUMAN (P30040) Endoplasmic reticulum protein ERp29 precurso... 28 3.7
DMD_HUMAN (P11532) Dystrophin 28 4.8
RS15_METTH (O27474) 30S ribosomal protein S15P/S13E 27 6.3
RPC2_ANASP (P22705) DNA-directed RNA polymerase beta' chain (EC ... 27 6.3
RPOA_YERPE (Q8ZJ87) DNA-directed RNA polymerase alpha chain (EC ... 27 8.2
RPOA_PHOLL (Q7MYH5) DNA-directed RNA polymerase alpha chain (EC ... 27 8.2
RPOA_ECOLI (P00574) DNA-directed RNA polymerase alpha chain (EC ... 27 8.2
ER29_RAT (P52555) Endoplasmic reticulum protein ERp29 precursor ... 27 8.2
DMD_CANFA (O97592) Dystrophin 27 8.2
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 37.7 bits (86), Expect = 0.005
Identities = 32/112 (28%), Positives = 53/112 (46%), Gaps = 3/112 (2%)
Query: 1 MSQEKMIEMADKATSLIVLCLVDKVSIDVKKETTMHGGHVGKVGVIVYDQVFDS*TIPKT 60
M E ++ ++A S I L L D V ++ E T G + + + + + K
Sbjct: 47 MKAEDWADLDERAASAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQ 106
Query: 61 KFLLIKYEVK*--SHHEEFNKILDNLENIEVHLEDEDKVILLL-CIPKSFES 109
+ L E SH FN ++ L N+ V +E+EDK ILLL +P S+++
Sbjct: 107 LYALHMSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDN 158
>SCG2_XENLA (O13263) Amiloride-sensitive sodium channel
gamma-2-subunit (Epithelial Na(+) channel gamma-2
subunit) (Gamma-2 ENAC) (Nonvoltage-gated sodium channel
1 gamma-2 subunit) (SCNEG2) (Gamma-2 NACH)
Length = 663
Score = 31.2 bits (69), Expect = 0.44
Identities = 16/36 (44%), Positives = 19/36 (52%)
Query: 1 MSQEKMIEMADKATSLIVLCLVDKVSIDVKKETTMH 36
+S E I M KA LIV CL D +S D + T H
Sbjct: 244 ISMEDKIAMGYKADELIVTCLFDGLSCDARNFTLFH 279
>ER29_MOUSE (P57759) Endoplasmic reticulum protein ERp29 precursor
Length = 262
Score = 28.5 bits (62), Expect = 2.8
Identities = 18/57 (31%), Positives = 31/57 (53%), Gaps = 4/57 (7%)
Query: 36 HGGH-VGKVGVIVYDQVFDS*TIPKTKFLLIKYEVK*SHHE---EFNKILDNLENIE 88
HGG + G + D V IPK+KF+L+K++ + + E EF ++ +N + E
Sbjct: 30 HGGSGLHTKGALPLDTVTFYKVIPKSKFVLVKFDTQYPYGEKQDEFKRLAENSASSE 86
>MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Actin
cross-linking family 7)
Length = 5327
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Query: 74 HEEFNKILDNLENIEVHLE-------DEDKVILLLCIPKSFE 108
HE + K++D LE+ E HL+ D DK+ L L K F+
Sbjct: 4486 HEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQ 4527
>MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, isoforms
1/2/3 (Actin cross-linking family protein 7) (Macrophin
1) (Trabeculin-alpha) (620 kDa actin-binding protein)
(ABP620)
Length = 5430
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Query: 74 HEEFNKILDNLENIEVHLE-------DEDKVILLLCIPKSFE 108
HE + K++D LE+ E HL+ D DK+ L L K F+
Sbjct: 4599 HEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQ 4640
>MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, isoform
4
Length = 5938
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Query: 74 HEEFNKILDNLENIEVHLE-------DEDKVILLLCIPKSFE 108
HE + K++D LE+ E HL+ D DK+ L L K F+
Sbjct: 5101 HEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQ 5142
>ER29_HUMAN (P30040) Endoplasmic reticulum protein ERp29 precursor
(ERp31) (ERp28)
Length = 261
Score = 28.1 bits (61), Expect = 3.7
Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 4/52 (7%)
Query: 36 HGGH-VGKVGVIVYDQVFDS*TIPKTKFLLIKYEVK*SHHE---EFNKILDN 83
HGG + G + D V IPK+KF+L+K++ + + E EF ++ +N
Sbjct: 28 HGGSGLHTKGALPLDTVTFYKVIPKSKFVLVKFDTQYPYGEKQDEFKRLAEN 79
>DMD_HUMAN (P11532) Dystrophin
Length = 3685
Score = 27.7 bits (60), Expect = 4.8
Identities = 12/27 (44%), Positives = 18/27 (66%)
Query: 75 EEFNKILDNLENIEVHLEDEDKVILLL 101
EE +++LD+LEN+ H ED I +L
Sbjct: 1300 EEISEVLDSLENLMRHSEDNPNQIRIL 1326
>RS15_METTH (O27474) 30S ribosomal protein S15P/S13E
Length = 134
Score = 27.3 bits (59), Expect = 6.3
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 4/64 (6%)
Query: 37 GGHVGKVGVIVYDQ----VFDS*TIPKTKFLLIKYEVK*SHHEEFNKILDNLENIEVHLE 92
G K+G+I+ DQ + T K +L K+E+K + E+ ++ NI HL+
Sbjct: 27 GNSTSKIGIILRDQHGIPSVKAVTGLKITQILEKHEMKPEYPEDLMNLIRKAVNIRDHLK 86
Query: 93 DEDK 96
+ K
Sbjct: 87 EHPK 90
>RPC2_ANASP (P22705) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta' chain)
(RNA polymerase beta' subunit)
Length = 1355
Score = 27.3 bits (59), Expect = 6.3
Identities = 15/38 (39%), Positives = 23/38 (60%)
Query: 3 QEKMIEMADKATSLIVLCLVDKVSIDVKKETTMHGGHV 40
Q + I++ADK +IV + +KV ID +TTM G +
Sbjct: 1135 QSQGIDIADKHIEVIVRQMTNKVRIDDGGDTTMLPGEL 1172
>RPOA_YERPE (Q8ZJ87) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 329
Score = 26.9 bits (58), Expect = 8.2
Identities = 14/39 (35%), Positives = 24/39 (60%)
Query: 63 LLIKYEVK*SHHEEFNKILDNLENIEVHLEDEDKVILLL 101
+L +Y K E+ +IL NL+ + V ++ +D+VIL L
Sbjct: 64 VLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL 102
>RPOA_PHOLL (Q7MYH5) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 329
Score = 26.9 bits (58), Expect = 8.2
Identities = 14/39 (35%), Positives = 24/39 (60%)
Query: 63 LLIKYEVK*SHHEEFNKILDNLENIEVHLEDEDKVILLL 101
+L +Y K E+ +IL NL+ + V ++ +D+VIL L
Sbjct: 64 VLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL 102
>RPOA_ECOLI (P00574) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 329
Score = 26.9 bits (58), Expect = 8.2
Identities = 14/39 (35%), Positives = 24/39 (60%)
Query: 63 LLIKYEVK*SHHEEFNKILDNLENIEVHLEDEDKVILLL 101
+L +Y K E+ +IL NL+ + V ++ +D+VIL L
Sbjct: 64 VLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL 102
>ER29_RAT (P52555) Endoplasmic reticulum protein ERp29 precursor
(ERp31)
Length = 260
Score = 26.9 bits (58), Expect = 8.2
Identities = 14/43 (32%), Positives = 25/43 (57%), Gaps = 3/43 (6%)
Query: 44 GVIVYDQVFDS*TIPKTKFLLIKYEVK*SHHE---EFNKILDN 83
G + D V IPK+KF+L+K++ + + E EF ++ +N
Sbjct: 37 GALPLDTVTFYKVIPKSKFVLVKFDTQYPYGEKQDEFKRLAEN 79
>DMD_CANFA (O97592) Dystrophin
Length = 3680
Score = 26.9 bits (58), Expect = 8.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 75 EEFNKILDNLENIEVHLEDEDKVILLL 101
EE ++LD+LEN+ H ED I +L
Sbjct: 1302 EEIAEVLDSLENLMQHSEDNPNQIRIL 1328
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.330 0.144 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,679,903
Number of Sequences: 164201
Number of extensions: 461685
Number of successful extensions: 1418
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1407
Number of HSP's gapped (non-prelim): 18
length of query: 119
length of database: 59,974,054
effective HSP length: 95
effective length of query: 24
effective length of database: 44,374,959
effective search space: 1064999016
effective search space used: 1064999016
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC122729.1