BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122728.13 + phase: 0
(164 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YM40_YEAST (Q03212) Hypothetical 62.5 kDa protein in ALD2-DDR48 ... 38 0.012
DDX1_DROME (Q9VNV3) ATP-dependent helicase DDX1 (DEAD box protei... 36 0.045
AMIB_ECOLI (P26365) N-acetylmuramoyl-L-alanine amidase amiB prec... 32 0.64
KYK1_DICDI (P18160) Non-receptor tyrosine kinase spore lysis A (... 31 1.1
YGX7_YEAST (P53076) Hypothetical 108.2 kDa protein in SAP4-OST5 ... 31 1.4
PKL1_HUMAN (Q16512) Protein kinase N1 (EC 2.7.1.37) (Protein kin... 30 2.4
SYD_VIBVY (Q7MJ74) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 30 3.2
SYD_VIBVU (Q8DAN7) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 30 3.2
PUR1_METTH (O26742) Amidophosphoribosyltransferase precursor (EC... 30 3.2
SYD_VIBPA (Q87QV2) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 29 4.2
YS97_MYCTU (Q10818) Hypothetical protein Rv2897c/MT2965/Mb2921c 29 5.4
PKL1_RAT (Q63433) Protein kinase N1 (EC 2.7.1.37) (Protein kinas... 29 5.4
PKL1_MOUSE (P70268) Serine/threonine-protein kinase N1 (EC 2.7.1... 29 5.4
NUD1_YEAST (P32336) NUD1 protein 29 5.4
NIRA_EMENI (P28348) Nitrogen assimilation transcription factor nirA 29 5.4
ALKH_TREPA (O83578) Putative KHG/KDPG aldolase [Includes: 4-hydr... 29 5.4
VLPE_MYCHR (Q49537) Variant surface antigen E precursor (VLPE pr... 28 7.1
FKB4_YEAST (Q06205) FK506-binding protein 4 (EC 5.2.1.8) (Peptid... 28 7.1
SYD_PHOLL (Q7MB41) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 28 9.3
ODO1_MOUSE (Q60597) 2-oxoglutarate dehydrogenase E1 component, m... 28 9.3
>YM40_YEAST (Q03212) Hypothetical 62.5 kDa protein in ALD2-DDR48
intergenic region
Length = 550
Score = 37.7 bits (86), Expect = 0.012
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVV----HCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
++G G+ R VFFT + + ++ H + +F LYP + +N+ + VNFG
Sbjct: 316 IVGIGYRSRSGTVFFTRNGKKLNEKSVGGHIRGWKFQY-LYPIIGSNVPCQIHVNFGTYG 374
Query: 74 FKYAPANAQR 83
F Y AN ++
Sbjct: 375 FVYIEANVKK 384
>DDX1_DROME (Q9VNV3) ATP-dependent helicase DDX1 (DEAD box protein
1)
Length = 727
Score = 35.8 bits (81), Expect = 0.045
Identities = 26/94 (27%), Positives = 41/94 (42%), Gaps = 6/94 (6%)
Query: 11 EWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFG 70
E G VIGC D + ++V FT + + + V + + + + + NFG
Sbjct: 183 EAFGKADVIGCLLDLKNQEVSFTKNGQNLGVAFRLPDNLAKETFYPAVVLKNAEMQFNFG 242
Query: 71 QNAFKYAPAN-----AQRTPNPCLVSPLVSSPAA 99
+ FKYAP N Q P +P ++ PAA
Sbjct: 243 KTDFKYAPGNGFVGACQAGPEHSKANP-ITGPAA 275
>AMIB_ECOLI (P26365) N-acetylmuramoyl-L-alanine amidase amiB
precursor (EC 3.5.1.28)
Length = 445
Score = 32.0 bits (71), Expect = 0.64
Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 2/58 (3%)
Query: 78 PANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWLNRTTTKGSHNNGNNI 135
P A+R P +V+P VS PA + +S + G I S+ + R + + N G+ I
Sbjct: 137 PVVAKRVETPAVVAPRVSEPARNPFKTESNR--TTGVISSNTVTRPAARATANTGDKI 192
>KYK1_DICDI (P18160) Non-receptor tyrosine kinase spore lysis A (EC
2.7.1.112) (Tyrosine-protein kinase 1)
Length = 1584
Score = 31.2 bits (69), Expect = 1.1
Identities = 15/46 (32%), Positives = 25/46 (53%), Gaps = 2/46 (4%)
Query: 13 IGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLA 58
I + VIGCG + + +FFT + + V + S PLYP+++
Sbjct: 816 IASSCVIGCGINTSTRDIFFTKNGHYLGVAFSRVT--SDPLYPSIS 859
Score = 30.4 bits (67), Expect = 1.9
Identities = 20/63 (31%), Positives = 33/63 (51%), Gaps = 4/63 (6%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLA-ANIDIMVLVN-FGQNAFK 75
V+GCG+D K ++FT + + V + LYPT+ N V++N FG +++
Sbjct: 123 VVGCGYDSSSKTLYFTKNGVYLGVAAQKVNLIG--LYPTVGLQNPGESVVINFFGPFSYR 180
Query: 76 YAP 78
AP
Sbjct: 181 GAP 183
>YGX7_YEAST (P53076) Hypothetical 108.2 kDa protein in SAP4-OST5
intergenic region
Length = 958
Score = 30.8 bits (68), Expect = 1.4
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 2/65 (3%)
Query: 8 EKAEWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLV 67
E A+ G D VIGCG + +FFT + +H+ + T+ P +A +
Sbjct: 528 EYAKPFGRDDVIGCGINFIDGSIFFTKNG--IHLGNAFTDLNDLEFVPYVALRPGNSIKT 585
Query: 68 NFGQN 72
NFG N
Sbjct: 586 NFGLN 590
>PKL1_HUMAN (Q16512) Protein kinase N1 (EC 2.7.1.37) (Protein kinase
C-like 1) (Protein-kinase C-related kinase 1) (Protein
kinase C-like PKN) (Serine-threonine protein kinase N)
Length = 942
Score = 30.0 bits (66), Expect = 2.4
Identities = 16/37 (43%), Positives = 20/37 (53%), Gaps = 3/37 (8%)
Query: 78 PANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGR 114
P+ Q TP P L SPL SP + +D K L +GR
Sbjct: 590 PSETQETPGPALCSPLRKSPLTL---EDFKFLAVLGR 623
>SYD_VIBVY (Q7MJ74) Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS)
Length = 592
Score = 29.6 bits (65), Expect = 3.2
Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 12/85 (14%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKYA 77
++G D ++ K F LD+ +F TP + LA +D +V++ G +
Sbjct: 502 ILGIDADEQKLKFGFLLDAL----------KFGTPPHAGLAFGLDRLVMLLCGTENIRDV 551
Query: 78 PANAQRTPNPCLV--SPLVSSPAAI 100
A + T CL+ +P V++PAA+
Sbjct: 552 IAFPKTTAAACLMTDAPSVANPAAL 576
>SYD_VIBVU (Q8DAN7) Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS)
Length = 592
Score = 29.6 bits (65), Expect = 3.2
Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 12/85 (14%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKYA 77
++G D ++ K F LD+ +F TP + LA +D +V++ G +
Sbjct: 502 ILGIDADEQKLKFGFLLDAL----------KFGTPPHAGLAFGLDRLVMLLCGTENIRDV 551
Query: 78 PANAQRTPNPCLV--SPLVSSPAAI 100
A + T CL+ +P V++PAA+
Sbjct: 552 IAFPKTTAAACLMTDAPSVANPAAL 576
>PUR1_METTH (O26742) Amidophosphoribosyltransferase precursor (EC
2.4.2.14) (Glutamine phosphoribosylpyrophosphate
amidotransferase) (ATASE) (GPATase)
Length = 474
Score = 29.6 bits (65), Expect = 3.2
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 8/61 (13%)
Query: 65 VLVNFGQNAFKYAPANAQRTPNPCLVSPLVSS--PAAIGYDDDSKELFSMGRIDSHWLNR 122
V +N G+ ++ PANA +V P+ S PAAIGY S + G I + ++ R
Sbjct: 272 VRLNIGEALYREHPANAD------VVVPVPDSSIPAAIGYSRASGIPYGEGLIKNRYVGR 325
Query: 123 T 123
T
Sbjct: 326 T 326
>SYD_VIBPA (Q87QV2) Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS)
Length = 592
Score = 29.3 bits (64), Expect = 4.2
Identities = 22/85 (25%), Positives = 41/85 (47%), Gaps = 12/85 (14%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKYA 77
++G + +Q K F LD+ +F TP + LA +D +V++ G +
Sbjct: 502 ILGIDAEEQQLKFGFLLDAL----------KFGTPPHAGLAFGLDRLVMLLCGTENIRDV 551
Query: 78 PANAQRTPNPCLV--SPLVSSPAAI 100
A + T CL+ +P +++PAA+
Sbjct: 552 IAFPKTTAAACLLTDAPSIANPAAL 576
>YS97_MYCTU (Q10818) Hypothetical protein Rv2897c/MT2965/Mb2921c
Length = 503
Score = 28.9 bits (63), Expect = 5.4
Identities = 24/73 (32%), Positives = 37/73 (49%), Gaps = 7/73 (9%)
Query: 81 AQRTPNPCLVSPLVSSPAAIGYDD-DSKELFSMGRIDSHWLNRTTTKGSHNNGNNISTMD 139
A+R P +V P + P A D D + + ++G++ S WL +GS I+T D
Sbjct: 129 AKRDGWPAVVVPADNLPEASLVDGIDVRGVRTLGQLQS-WL-----RGSTGLAGRITTAD 182
Query: 140 FDEESEADLFEIV 152
ES ADL ++V
Sbjct: 183 TTPESAADLADVV 195
>PKL1_RAT (Q63433) Protein kinase N1 (EC 2.7.1.37) (Protein kinase
C-like 1) (Protein-kinase C-related kinase 1) (Protein
kinase C-like PKN) (Serine-threonine protein kinase N)
(Protease-activated kinase 1) (PAK-1)
Length = 946
Score = 28.9 bits (63), Expect = 5.4
Identities = 16/37 (43%), Positives = 20/37 (53%), Gaps = 3/37 (8%)
Query: 78 PANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGR 114
P+ Q TP P L SPL SP + +D K L +GR
Sbjct: 594 PSETQETPGPGLCSPLRKSPLTL---EDFKFLAVLGR 627
>PKL1_MOUSE (P70268) Serine/threonine-protein kinase N1 (EC
2.7.1.37) (Protein kinase C-like 1) (Protein-kinase
C-related kinase 1) (Protein kinase C-like PKN)
(Serine-threonine protein kinase N)
Length = 946
Score = 28.9 bits (63), Expect = 5.4
Identities = 16/37 (43%), Positives = 20/37 (53%), Gaps = 3/37 (8%)
Query: 78 PANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGR 114
P+ Q TP P L SPL SP + +D K L +GR
Sbjct: 594 PSETQETPGPGLCSPLRKSPLTL---EDFKFLAVLGR 627
>NUD1_YEAST (P32336) NUD1 protein
Length = 851
Score = 28.9 bits (63), Expect = 5.4
Identities = 23/85 (27%), Positives = 33/85 (38%), Gaps = 15/85 (17%)
Query: 95 SSPAAIGYD------DDSKELFSMGRIDSHWLNRTTTKGSHNNGNN---------ISTMD 139
S PAA +D K + +G S+ N+ ++NN NN T +
Sbjct: 194 SGPAAEAHDVFDGILQKQKSNYLVGSYPSNSNNKNNNNNNNNNNNNSININNKDNARTKE 253
Query: 140 FDEESEADLFEIVIDGSRKSPSTTS 164
DEE ++ FE S S T S
Sbjct: 254 EDEEDTSNSFEFSSSSSMSSSQTQS 278
>NIRA_EMENI (P28348) Nitrogen assimilation transcription factor nirA
Length = 892
Score = 28.9 bits (63), Expect = 5.4
Identities = 17/62 (27%), Positives = 28/62 (44%), Gaps = 3/62 (4%)
Query: 103 DDDSKELFSMGRIDSHWLNRTTTKGSHNNGNNISTMDFDEESEADLFEIVIDGSRKSPST 162
+D ++ L + + S WL+ H + T F+ E + +V+DGSRK
Sbjct: 127 EDVAQSLVNQEKKSSGWLSNAVI---HEENDIAQTDQFESELAGKMSNLVLDGSRKFIGG 183
Query: 163 TS 164
TS
Sbjct: 184 TS 185
>ALKH_TREPA (O83578) Putative KHG/KDPG aldolase [Includes:
4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16)
(2-keto-4-hydroxyglutarate aldolase) (KHG-aldolase);
2-dehydro-3-deoxy-phosphogluconate aldolase (EC
4.1.2.14) (Phospho-2-dehydro-3-deoxygluconate a
Length = 210
Score = 28.9 bits (63), Expect = 5.4
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 15/76 (19%)
Query: 4 VFESEKAEWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDI 63
V ++++A+ G V+ GF+PR V HC P+ P +A+ +I
Sbjct: 73 VEQAQQAQAAGAQFVVSPGFNPRV-------------VAHCLGH--GVPIIPGIASATEI 117
Query: 64 MVLVNFGQNAFKYAPA 79
+ FG + K+ PA
Sbjct: 118 ERALEFGISVVKFFPA 133
>VLPE_MYCHR (Q49537) Variant surface antigen E precursor (VLPE
prolipoprotein)
Length = 243
Score = 28.5 bits (62), Expect = 7.1
Identities = 28/100 (28%), Positives = 41/100 (41%), Gaps = 14/100 (14%)
Query: 68 NFGQNAFKYAPANAQRTP-NPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWLNRTTTK 126
N N+ P P NP P S+P+ D +S+ N TT+
Sbjct: 83 NGSSNSSVSTPDGQHSNPSNPTTSDPKESNPSNPTTSDPK---------ESNPSNPTTSD 133
Query: 127 GSHNNGNNISTMDFDEESEADLFEIVIDGSRKSPS--TTS 164
G H+N +N +T D E + ++ DG +PS TTS
Sbjct: 134 GQHSNPSNPTTSDPKESNPSN--PTTSDGQHSNPSNPTTS 171
>FKB4_YEAST (Q06205) FK506-binding protein 4 (EC 5.2.1.8)
(Peptidyl-prolyl cis-trans isomerase) (PPIase)
(Rotamase)
Length = 392
Score = 28.5 bits (62), Expect = 7.1
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 7/90 (7%)
Query: 62 DIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWLN 121
D++ L + N Y+P A P + +++ +DDD K S RI N
Sbjct: 3 DMLPLATYSLNVEPYSPTPALNFKIPVTIRITMAAIDPEPFDDDKKP--STLRIIKR--N 58
Query: 122 RTTTKGSHNNGNNISTMDFDE---ESEADL 148
T+G + N +N ++ DE E EAD+
Sbjct: 59 PELTRGEYYNQDNNDGLEEDESESEQEADV 88
>SYD_PHOLL (Q7MB41) Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS)
Length = 590
Score = 28.1 bits (61), Expect = 9.3
Identities = 21/86 (24%), Positives = 42/86 (48%), Gaps = 12/86 (13%)
Query: 17 KVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKY 76
+++G +Q+K F LD+ +F TP + LA +D +V++ G + +
Sbjct: 501 RILGINEQEQQEKFGFLLDAL----------KFGTPPHAGLAFGLDRLVMLLTGTDNIRD 550
Query: 77 APANAQRTPNPCLV--SPLVSSPAAI 100
A + T CL+ +P ++PA++
Sbjct: 551 VIAFPKTTAAACLMTEAPSYANPASL 576
>ODO1_MOUSE (Q60597) 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase)
Length = 1019
Score = 28.1 bits (61), Expect = 9.3
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Query: 47 EEFSTPLYPTLAANIDIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVSSPAAIGYDDDS 106
E F P+ + + V+VN Q F P A+ +P P V+ +V++P DD
Sbjct: 418 ETFHLSDLPSYTTHGTVHVVVN-NQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDP 476
Query: 107 KELFSMGRIDSHWLN 121
+ + + ++ + W N
Sbjct: 477 EAVMYVCKVAAEWRN 491
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.132 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,132,915
Number of Sequences: 164201
Number of extensions: 830307
Number of successful extensions: 1731
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 1717
Number of HSP's gapped (non-prelim): 30
length of query: 164
length of database: 59,974,054
effective HSP length: 102
effective length of query: 62
effective length of database: 43,225,552
effective search space: 2679984224
effective search space used: 2679984224
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC122728.13