BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122727.11 + phase: 0 /pseudo/partial
(359 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YC47_HELPJ (Q9ZJY0) Protein JHP1168 33 0.85
PURA_BRUAB (P52004) Adenylosuccinate synthetase (EC 6.3.4.4) (IM... 33 0.85
MDR2_MOUSE (P21440) Multidrug resistance protein 2 (P-glycoprote... 33 0.85
PURA_BRUSU (P65879) Adenylosuccinate synthetase (EC 6.3.4.4) (IM... 33 1.1
PURA_BRUME (P65878) Adenylosuccinate synthetase (EC 6.3.4.4) (IM... 33 1.1
YC47_HELPY (O25842) Protein HP1247 33 1.5
MDR3_CRIGR (P23174) Multidrug resistance protein 3 (P-glycoprote... 33 1.5
SYS_WOLSU (Q7M9J5) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 32 2.5
LUXD_SHEHA (Q9AJA7) Acyl transferase (EC 2.3.1.-) (ACT) (Myristo... 32 2.5
IF2_THEMA (Q9WZN3) Translation initiation factor IF-2 32 2.5
GCSP_STRAW (Q827D7) Glycine dehydrogenase [decarboxylating] (EC ... 32 2.5
CENF_HUMAN (P49454) CENP-F kinetochore protein (Centromere prote... 32 2.5
RRPO_TCV (P17460) Probable RNA-directed RNA polymerase (EC 2.7.7... 31 4.2
VHS_PRVKA (P36314) Virion host shutoff protein 31 5.5
VE2_HPV24 (P50770) Regulatory protein E2 31 5.5
HIS4_METMP (P62357) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 31 5.5
YHAP_BACSU (O07523) Hypothetical protein yhaP 30 7.2
TCMO_RUTGR (Q9AR74) Trans-cinnamate 4-monooxygenase (EC 1.14.13.... 30 7.2
REPA_NEIGO (P17492) Replication protein 30 7.2
PSTB_SULSO (Q97ZT9) Phosphate import ATP-binding protein pstB (E... 30 7.2
>YC47_HELPJ (Q9ZJY0) Protein JHP1168
Length = 340
Score = 33.5 bits (75), Expect = 0.85
Identities = 21/71 (29%), Positives = 38/71 (52%), Gaps = 7/71 (9%)
Query: 136 DELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 195
D+L L P+T++ ++E L D++ L+LG +KK+++ + DYL K G +
Sbjct: 195 DKLAVLNAPITLEDIQE-------LSSNAGDMDLQKLILGLFLKKSVLDIYDYLLKEGKK 247
Query: 196 KSTITQFLRTY 206
+ I + L Y
Sbjct: 248 DADILRGLERY 258
>PURA_BRUAB (P52004) Adenylosuccinate synthetase (EC 6.3.4.4)
(IMP--aspartate ligase) (AdSS) (AMPSase) (Fragment)
Length = 402
Score = 33.5 bits (75), Expect = 0.85
Identities = 61/246 (24%), Positives = 95/246 (37%), Gaps = 60/246 (24%)
Query: 147 VDVMKERVDFL------HSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTIT 200
VD + ER D + H+ G T+ ++ G S K +++P GKL V
Sbjct: 20 VDWLSERADVIVRYQGGHNAGHTL-------VIDGVSYKLSLLPSGLVRGKLSV------ 66
Query: 201 QFLRTYPQVLHASVVVD---LVPVVKYL--QGMDIKPDDIPRVLERYPEVLGFKLE---- 251
+ VVVD V V+ L QG+D+ PD + RV E P +L E
Sbjct: 67 ---------IGNGVVVDPHHFVAEVEKLRGQGIDVTPD-VLRVAENAPLILSIHRELDAM 116
Query: 252 ----------GTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLG 301
GT + VGRR + I PE L +V R++ +G
Sbjct: 117 REGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMG 176
Query: 302 IPRLAIARLI--------ETQPYI--LGFDLDEKVKPNVKSLEEFNVRETSLASIIAQYP 351
+ +A+ ++ + PYI + LDE+ K + L F R L + YP
Sbjct: 177 LEEIAVETILTELTSVADQILPYIDQVWRVLDERRKRGARIL--FEGRRALLDNDHGTYP 234
Query: 352 DIIGTD 357
+ ++
Sbjct: 235 FVTSSN 240
>MDR2_MOUSE (P21440) Multidrug resistance protein 2 (P-glycoprotein 2)
Length = 1276
Score = 33.5 bits (75), Expect = 0.85
Identities = 31/120 (25%), Positives = 49/120 (40%), Gaps = 13/120 (10%)
Query: 98 ASSLYAGYARPSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFL 157
ASS YA+ LS R+ + + G G+ PD+ +G V V F
Sbjct: 987 ASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEG-------SVTFNEVVFN 1039
Query: 158 HSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVD 217
+ N P++ G S++ L +G G KST+ Q L + + SV++D
Sbjct: 1040 YPTRA------NVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLD 1093
>PURA_BRUSU (P65879) Adenylosuccinate synthetase (EC 6.3.4.4)
(IMP--aspartate ligase) (AdSS) (AMPSase)
Length = 429
Score = 33.1 bits (74), Expect = 1.1
Identities = 57/224 (25%), Positives = 87/224 (38%), Gaps = 58/224 (25%)
Query: 147 VDVMKERVDFL------HSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTIT 200
VD + ER D + H+ G T+ ++ G S K +++P GKL V
Sbjct: 20 VDWLSERADVIVRYQGGHNAGHTL-------VIDGVSYKLSLLPSGLVRGKLSV------ 66
Query: 201 QFLRTYPQVLHASVVVD---LVPVVKYL--QGMDIKPDDIPRVLERYPEVLGFKLE---- 251
+ VVVD V V+ L QG+D+ PD + RV E P +L E
Sbjct: 67 ---------IGNGVVVDPHHFVAEVEKLRGQGIDVTPD-VLRVAENAPLILSIHRELDAM 116
Query: 252 ----------GTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLG 301
GT + VGRR + I PE L +V R++ +G
Sbjct: 117 REGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMG 176
Query: 302 IPRLAIARLI--------ETQPYI--LGFDLDEKVKPNVKSLEE 335
+ +A+ ++ + PYI + LDE+ K + L E
Sbjct: 177 LEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFE 220
>PURA_BRUME (P65878) Adenylosuccinate synthetase (EC 6.3.4.4)
(IMP--aspartate ligase) (AdSS) (AMPSase)
Length = 429
Score = 33.1 bits (74), Expect = 1.1
Identities = 57/224 (25%), Positives = 87/224 (38%), Gaps = 58/224 (25%)
Query: 147 VDVMKERVDFL------HSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTIT 200
VD + ER D + H+ G T+ ++ G S K +++P GKL V
Sbjct: 20 VDWLSERADVIVRYQGGHNAGHTL-------VIDGVSYKLSLLPSGLVRGKLSV------ 66
Query: 201 QFLRTYPQVLHASVVVD---LVPVVKYL--QGMDIKPDDIPRVLERYPEVLGFKLE---- 251
+ VVVD V V+ L QG+D+ PD + RV E P +L E
Sbjct: 67 ---------IGNGVVVDPHHFVAEVEKLRGQGIDVTPD-VLRVAENAPLILSIHRELDAM 116
Query: 252 ----------GTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLG 301
GT + VGRR + I PE L +V R++ +G
Sbjct: 117 REGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMG 176
Query: 302 IPRLAIARLI--------ETQPYI--LGFDLDEKVKPNVKSLEE 335
+ +A+ ++ + PYI + LDE+ K + L E
Sbjct: 177 LEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFE 220
>YC47_HELPY (O25842) Protein HP1247
Length = 340
Score = 32.7 bits (73), Expect = 1.5
Identities = 21/71 (29%), Positives = 37/71 (51%), Gaps = 7/71 (9%)
Query: 136 DELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 195
D+L L P+T++ ++E L D++ L+LG +KK+ + + DYL K G +
Sbjct: 195 DKLAVLNAPITLEDIQE-------LSSNAGDMDLQKLILGLFLKKSALDIYDYLLKEGKK 247
Query: 196 KSTITQFLRTY 206
+ I + L Y
Sbjct: 248 DADILRGLERY 258
>MDR3_CRIGR (P23174) Multidrug resistance protein 3 (P-glycoprotein 3)
Length = 1281
Score = 32.7 bits (73), Expect = 1.5
Identities = 30/120 (25%), Positives = 49/120 (40%), Gaps = 13/120 (10%)
Query: 98 ASSLYAGYARPSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFL 157
ASS YA+ LS R+ + + G G+ PD+ +G V V F
Sbjct: 992 ASSFAPDYAKAKLSAAHLFSLFERQPLIDSYSGEGLWPDKFEG-------SVTFNEVVFN 1044
Query: 158 HSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVD 217
+ N P++ G S++ L +G G KST+ Q L + + +V++D
Sbjct: 1045 YPTRA------NMPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLD 1098
>SYS_WOLSU (Q7M9J5) Seryl-tRNA synthetase (EC 6.1.1.11)
(Serine--tRNA ligase) (SerRS)
Length = 428
Score = 32.0 bits (71), Expect = 2.5
Identities = 19/71 (26%), Positives = 35/71 (48%), Gaps = 2/71 (2%)
Query: 75 RTSAADSQGSKRDSGEIQRKRRGASSLYAGYAR--PSLSEMKKDKATLRKVVYEFLRGIG 132
R A + KR+S E+Q ++ S L+ Y + + +K + TL+ + E L +
Sbjct: 32 RLLAGQYKAKKRESEELQAEQNAKSKLFGQYKKEGKEIDSLKAELDTLKIRLSEILPEVS 91
Query: 133 IVPDELDGLEL 143
++ EL+ L L
Sbjct: 92 VLERELESLAL 102
>LUXD_SHEHA (Q9AJA7) Acyl transferase (EC 2.3.1.-) (ACT)
(Myristoyl-ACP-specific thioesterase) (C14ACP-TE)
Length = 308
Score = 32.0 bits (71), Expect = 2.5
Identities = 29/105 (27%), Positives = 49/105 (46%), Gaps = 11/105 (10%)
Query: 153 RVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRK------STITQFLRTY 206
R D LH +GL+ DIN + + +G K +++ V+++L GV K S +
Sbjct: 69 RYDSLHHVGLSSGDINEFTMSIG---KDSLLIVIEWLKGRGVNKLGLIAASLSARIAYEI 125
Query: 207 PQVLHASVVVDLVPVVKYLQGMD--IKPDDIPRVLERYPEVLGFK 249
+ S +V V VV ++ +K D + +E PE L F+
Sbjct: 126 SNDVDLSFLVTAVGVVNLRDTLERSLKYDYLQLEIEDLPEDLDFE 170
>IF2_THEMA (Q9WZN3) Translation initiation factor IF-2
Length = 690
Score = 32.0 bits (71), Expect = 2.5
Identities = 24/98 (24%), Positives = 47/98 (47%), Gaps = 10/98 (10%)
Query: 109 SLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMK-ERVDFLHSLGLTIEDI 167
+++++ K A + K E + +G++P+E G + V + + VD L + L + ++
Sbjct: 285 AINKIDKPNANVEKTKQELVEKLGLIPEEWGGDTIVVPISARTGQGVDELLEMILLVAEM 344
Query: 168 NN---YP------LVLGCSVKKNMVPVLDYLGKLGVRK 196
N YP +++ + K M PV + K GV K
Sbjct: 345 NEIKCYPEGPARAVIIESKLDKKMGPVASAIVKDGVLK 382
>GCSP_STRAW (Q827D7) Glycine dehydrogenase [decarboxylating] (EC
1.4.4.2) (Glycine decarboxylase) (Glycine cleavage
system P-protein)
Length = 961
Score = 32.0 bits (71), Expect = 2.5
Identities = 23/65 (35%), Positives = 30/65 (45%), Gaps = 6/65 (9%)
Query: 262 IGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLGIPRLAIARLIE----TQPYI 317
I G+ RE+ G+L ++P G R IKP VE LG A L+ T P
Sbjct: 209 IPAGIAEREINGVLIQYPGASG--AVRDIKPLVEQAHELGAVVTVAADLLALTLLTSPGE 266
Query: 318 LGFDL 322
LG D+
Sbjct: 267 LGADI 271
>CENF_HUMAN (P49454) CENP-F kinetochore protein (Centromere protein F)
(Mitosin) (AH antigen)
Length = 3210
Score = 32.0 bits (71), Expect = 2.5
Identities = 40/173 (23%), Positives = 73/173 (42%), Gaps = 20/173 (11%)
Query: 48 QLPCNYIRKIRFITKLQCSVAGRTDSYRTSAADSQGSKRDSGEIQRKRRGASSLYAGYAR 107
+L + K+ F+ K+ A T+ R +Q + E+Q + G + AG +
Sbjct: 2692 ELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQ----KTAELQEELSGEKNRLAGELQ 2747
Query: 108 PSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDI 167
L E+K K L+++ L+ EL ++D M + D + G E+I
Sbjct: 2748 LLLEEIKSSKDQLKELT-------------LENSELKKSLDCMHK--DQVEKEGKVREEI 2792
Query: 168 NNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHA-SVVVDLV 219
Y L L + KK+ +LD + V T + L + + L + + +DL+
Sbjct: 2793 AEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLL 2845
>RRPO_TCV (P17460) Probable RNA-directed RNA polymerase (EC
2.7.7.48) (Protein p88) [Contains: Protein p28]
Length = 775
Score = 31.2 bits (69), Expect = 4.2
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 1/55 (1%)
Query: 88 SGEIQRKRRGASSLYAGYARPSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLE 142
+G+ ++K + S +G+ R S + M+ K + + F RG G PDE + LE
Sbjct: 688 AGDYKKKMQDVS-FDSGFYRLSKNGMRGSKDVSQDARFSFYRGFGYTPDEQEALE 741
>VHS_PRVKA (P36314) Virion host shutoff protein
Length = 365
Score = 30.8 bits (68), Expect = 5.5
Identities = 21/84 (25%), Positives = 41/84 (48%), Gaps = 9/84 (10%)
Query: 172 LVLGCSVKKNMVPVL-------DYLGKLGVRKST-ITQFLRTYPQVLHASVVVDLVPVVK 223
++ GC + ++ PV D L LGV S + F+R + + A V + V +
Sbjct: 175 ILTGCDMILDIAPVFPLVLRCRDVLASLGVDYSEFLAAFVRCHTDLHRAPDVDSVQQVAE 234
Query: 224 YLQGMDIKPDDIP-RVLERYPEVL 246
L+G ++ P+D+ + R P+++
Sbjct: 235 SLEGREVAPEDVKLKYASRCPDIM 258
>VE2_HPV24 (P50770) Regulatory protein E2
Length = 467
Score = 30.8 bits (68), Expect = 5.5
Identities = 28/93 (30%), Positives = 44/93 (47%), Gaps = 10/93 (10%)
Query: 55 RKIRFITKLQCS---VAGRTDSYRTSAADSQGSKRDSGEIQRKRRGASSLYAGYAR---- 107
R+ R T+ +CS R+ S R S + S+G++R G+ R +RG S+ G R
Sbjct: 268 RRARSRTRSRCSSTQTRSRSTS-RRSRSTSRGNRRCRGDTPRGQRGVSTSSRGRGRGSRR 326
Query: 108 --PSLSEMKKDKATLRKVVYEFLRGIGIVPDEL 138
S S + KA+ R +R GI P ++
Sbjct: 327 SSSSSSPTPRTKASQRGCDTRSVRDSGISPGDV 359
>HIS4_METMP (P62357)
1-(5-phosphoribosyl)-5-[(5-
phosphoribosylamino)methylideneamino]
imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(Phosphoribosylformimino-5-aminoimidazole carboxamide
ribotide isomerase)
Length = 241
Score = 30.8 bits (68), Expect = 5.5
Identities = 19/62 (30%), Positives = 34/62 (54%), Gaps = 6/62 (9%)
Query: 128 LRGIGIVPDE--LDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPV 185
L GI I P + +D L++P+ +D L L +++I Y +V+G ++ KNM+ +
Sbjct: 175 LNGINIEPTKELVDNLKIPIVASGGVTTIDDL----LKLKEIGVYGVVVGSAIYKNMINL 230
Query: 186 LD 187
D
Sbjct: 231 KD 232
>YHAP_BACSU (O07523) Hypothetical protein yhaP
Length = 419
Score = 30.4 bits (67), Expect = 7.2
Identities = 28/108 (25%), Positives = 53/108 (48%), Gaps = 5/108 (4%)
Query: 160 LGLTIEDINNY--PLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVD 217
LG++ E I++ P+ +G K + LG+ V I F+ + +++AS++
Sbjct: 149 LGVSQETISSLYAPVTVGQKALKEGAKSEEELGQT-VGLVYIMLFVIYFSVIMYASMIAM 207
Query: 218 LVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIG 265
V K + M+I +P + + + ++LG L G T +A +IG G
Sbjct: 208 EVATEKSSRVMEILISSMPPIQQMFAKLLGIGLVGI--TQLAIIIGAG 253
>TCMO_RUTGR (Q9AR74) Trans-cinnamate 4-monooxygenase (EC 1.14.13.11)
(Cinnamic acid 4-hydroxylase) (CA4H) (C4H) (P450C4H)
(Cytochrome P450 73)
Length = 506
Score = 30.4 bits (67), Expect = 7.2
Identities = 15/32 (46%), Positives = 21/32 (64%), Gaps = 1/32 (3%)
Query: 262 IGIGVGRRELGGILTRFPEILGMRVGRVIKPF 293
I GVGRR GI+ P ILG+ +GR+++ F
Sbjct: 438 IPFGVGRRSCPGIILALP-ILGITIGRMVQNF 468
>REPA_NEIGO (P17492) Replication protein
Length = 328
Score = 30.4 bits (67), Expect = 7.2
Identities = 17/79 (21%), Positives = 35/79 (43%), Gaps = 9/79 (11%)
Query: 162 LTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPV 221
L+I++I L +G K+ + D+ T+ F+R +P++ + +
Sbjct: 20 LSIDEIRILALTIGTMDPKSNQKIFDF---------TVADFVREFPEISQDNAYKQIQAA 70
Query: 222 VKYLQGMDIKPDDIPRVLE 240
+K + +K +D RV E
Sbjct: 71 IKRIYDRSVKTEDKDRVTE 89
>PSTB_SULSO (Q97ZT9) Phosphate import ATP-binding protein pstB (EC
3.6.3.27) (Phosphate-transporting ATPase) (ABC phosphate
transporter)
Length = 257
Score = 30.4 bits (67), Expect = 7.2
Identities = 16/47 (34%), Positives = 25/47 (53%)
Query: 100 SLYAGYARPSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVT 146
S+Y A P S K+K ++K+V E LR +G+ + D L P +
Sbjct: 106 SIYDNIAYPLKSHGIKEKREIKKIVEECLRKVGLWKEVYDRLNSPAS 152
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.141 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,956,044
Number of Sequences: 164201
Number of extensions: 1816166
Number of successful extensions: 4715
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 4707
Number of HSP's gapped (non-prelim): 31
length of query: 359
length of database: 59,974,054
effective HSP length: 111
effective length of query: 248
effective length of database: 41,747,743
effective search space: 10353440264
effective search space used: 10353440264
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)
Medicago: description of AC122727.11