BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122727.10 - phase: 1 /pseudo
(136 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5) (Methy... 32 0.52
MOKC_SCHPO (Q9UUL4) Cell wall alpha-1,3-glucan synthase mok12 (E... 30 1.5
C1JB_BACTU (Q45716) Pesticidial crystal protein cry1Jb (Insectic... 29 3.4
BIOB_ARATH (P54967) Biotin synthase (EC 2.8.1.6) (Biotin synthet... 29 3.4
MSRB_BACHD (Q9KCX2) Peptide methionine sulfoxide reductase msrB ... 28 4.4
HS72_ANOAL (P41826) Heat shock protein 70 A2 28 5.8
HS71_ANOAL (P41825) Heat shock protein 70 A1 28 5.8
FMO2_MACMU (Q28505) Dimethylaniline monooxygenase [N-oxide-formi... 28 5.8
C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb (Insectic... 28 5.8
YG3H_YEAST (P53285) Hypothetical 54.5 kDa protein in CBF2-SKN1 i... 28 7.5
YE61_METJA (Q58856) Hypothetical protein MJ1461 28 7.5
GSH1_PSESY (P61379) Glutamate--cysteine ligase (EC 6.3.2.2) (Gam... 28 7.5
C1JA_BACTU (Q45738) Pesticidial crystal protein cry1Ja (Insectic... 28 7.5
ST12_ARATH (Q9MAX3) Sulfate transporter 1.2 27 9.8
LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only... 27 9.8
GLS3_YEAST (Q04952) 1,3-beta-glucan synthase component FKS3 (EC ... 27 9.8
>LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5)
(Methylglyoxalase) (Aldoketomutase) (Glyoxalase I)
Length = 219
Score = 31.6 bits (70), Expect = 0.52
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Query: 60 SMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 101
S PS + R+Q R W D + K + D KK WL + H++G++
Sbjct: 82 SPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEEHEEGKK 126
>MOKC_SCHPO (Q9UUL4) Cell wall alpha-1,3-glucan synthase mok12 (EC
2.4.1.183)
Length = 2352
Score = 30.0 bits (66), Expect = 1.5
Identities = 14/43 (32%), Positives = 21/43 (48%)
Query: 39 LNFLRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVV 81
LN+ R F N ++ GY + PS Y + R WS D++
Sbjct: 964 LNYSRQFPNLFVQGAFNRFGYDGSMPSKMSYNLENRTWSYDLI 1006
>C1JB_BACTU (Q45716) Pesticidial crystal protein cry1Jb
(Insecticidal delta-endotoxin CryIJ(b)) (Crystaline
entomocidal protoxin) (134 kDa crystal protein)
Length = 1170
Score = 28.9 bits (63), Expect = 3.4
Identities = 11/28 (39%), Positives = 18/28 (64%)
Query: 99 GRRWGHITTNISECFNNVLKGGPQSSNH 126
G RWG TTN+++ +N +K + S+H
Sbjct: 178 GERWGLTTTNVNDIYNRQVKRIHEYSDH 205
>BIOB_ARATH (P54967) Biotin synthase (EC 2.8.1.6) (Biotin
synthetase)
Length = 378
Score = 28.9 bits (63), Expect = 3.4
Identities = 18/56 (32%), Positives = 25/56 (44%)
Query: 75 DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 130
DWS D +K V D+P L Q R H + +C +K G S + + CP
Sbjct: 47 DWSRDEIKSVYDSPLLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCP 102
>MSRB_BACHD (Q9KCX2) Peptide methionine sulfoxide reductase msrB (EC
1.8.4.6)
Length = 143
Score = 28.5 bits (62), Expect = 4.4
Identities = 22/72 (30%), Positives = 32/72 (43%), Gaps = 4/72 (5%)
Query: 44 TFKNTEMKKKVRNMGYSMTQPSITH--YRNQIRDWSDD--VVKWVDDNPKKQWLQAHDQG 99
T +N E+KKK+ + Y +TQ + T +RN+ D + V V P +D G
Sbjct: 2 TEQNEELKKKLTPLQYEVTQNNGTEPPFRNEYYDLEAEGIYVDIVSGKPLFSSKDKYDAG 61
Query: 100 RRWGHITTNISE 111
W T I E
Sbjct: 62 CGWPSFTKPIDE 73
>HS72_ANOAL (P41826) Heat shock protein 70 A2
Length = 640
Score = 28.1 bits (61), Expect = 5.8
Identities = 12/56 (21%), Positives = 26/56 (46%)
Query: 70 RNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSN 125
R ++D D+ ++W+D N + + Q + + + I + GGPQ ++
Sbjct: 565 RRTVQDRCDETLRWIDGNTMAEKEEYEHQMQELSRVCSPIMTKLHQQAAGGPQPTS 620
>HS71_ANOAL (P41825) Heat shock protein 70 A1
Length = 640
Score = 28.1 bits (61), Expect = 5.8
Identities = 12/56 (21%), Positives = 26/56 (46%)
Query: 70 RNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSN 125
R ++D D+ ++W+D N + + Q + + + I + GGPQ ++
Sbjct: 565 RRTVQDRCDETLRWIDGNTMAEKEEYEHQMQELSRVCSPIMTKLHQQAAGGPQPTS 620
>FMO2_MACMU (Q28505) Dimethylaniline monooxygenase [N-oxide-forming]
2 (EC 1.14.13.8) (Pulmonary flavin-containing
monooxygenase 2) (FMO 2) (Dimethylaniline oxidase 2)
(FMO 1B1)
Length = 534
Score = 28.1 bits (61), Expect = 5.8
Identities = 16/60 (26%), Positives = 26/60 (42%), Gaps = 1/60 (1%)
Query: 41 FLRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRD-WSDDVVKWVDDNPKKQWLQAHDQG 99
F+ T T + +V GY T +R+ +R+ VVKW+ + QW + G
Sbjct: 210 FISTRHGTWVMSRVSEDGYPWDSVFHTRFRSMLRNVLPRTVVKWMIEQQMNQWFNHENYG 269
>C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb
(Insecticidal delta-endotoxin CryIC(b)) (Crystaline
entomocidal protoxin) (133 kDa crystal protein)
Length = 1176
Score = 28.1 bits (61), Expect = 5.8
Identities = 10/28 (35%), Positives = 18/28 (63%)
Query: 99 GRRWGHITTNISECFNNVLKGGPQSSNH 126
G RWG T N++E +N +++ + +NH
Sbjct: 178 GARWGLTTINVNENYNRLIRHIDEYANH 205
>YG3H_YEAST (P53285) Hypothetical 54.5 kDa protein in CBF2-SKN1
intergenic region
Length = 467
Score = 27.7 bits (60), Expect = 7.5
Identities = 9/29 (31%), Positives = 15/29 (51%)
Query: 71 NQIRDWSDDVVKWVDDNPKKQWLQAHDQG 99
N + DW +V++ P+ W+ AH G
Sbjct: 224 NHVGDWEHSLVRFYKGEPQYLWMSAHGGG 252
>YE61_METJA (Q58856) Hypothetical protein MJ1461
Length = 199
Score = 27.7 bits (60), Expect = 7.5
Identities = 13/49 (26%), Positives = 24/49 (48%)
Query: 41 FLRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPK 89
F+ F+N K RN S T + N++++W + + ++ DD K
Sbjct: 48 FIERFRNNPCICKNRNNHSSCTTNACGEIENRMKNWVEKLFEYSDDEEK 96
>GSH1_PSESY (P61379) Glutamate--cysteine ligase (EC 6.3.2.2)
(Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
Length = 535
Score = 27.7 bits (60), Expect = 7.5
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 4/65 (6%)
Query: 53 KVRNMGY-SMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQA--HDQGRRWGHITTNI 109
++ ++GY S Q +T N + ++D + K V P K +++ HDQ W + TN+
Sbjct: 242 RMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVA-TPYKPYVEVGTHDQNGEWVQLNTNV 300
Query: 110 SECFN 114
+ N
Sbjct: 301 LQIEN 305
>C1JA_BACTU (Q45738) Pesticidial crystal protein cry1Ja
(Insecticidal delta-endotoxin CryIJ(a)) (Crystaline
entomocidal protoxin) (133 kDa crystal protein)
Length = 1167
Score = 27.7 bits (60), Expect = 7.5
Identities = 10/28 (35%), Positives = 17/28 (60%)
Query: 99 GRRWGHITTNISECFNNVLKGGPQSSNH 126
G RWG T N+++ +N ++ + SNH
Sbjct: 178 GERWGLTTKNVNDIYNRQIREIHEYSNH 205
>ST12_ARATH (Q9MAX3) Sulfate transporter 1.2
Length = 653
Score = 27.3 bits (59), Expect = 9.8
Identities = 14/37 (37%), Positives = 23/37 (61%)
Query: 11 FQIDTSQSKVLLGV*TVIGTMSSAFDT*LNFLRTFKN 47
+QID ++ V LG+ V+G+MSS + +F R+ N
Sbjct: 365 YQIDGNKEMVALGMMNVVGSMSSCYVATGSFSRSAVN 401
>LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only
protein 20)
Length = 1683
Score = 27.3 bits (59), Expect = 9.8
Identities = 11/39 (28%), Positives = 19/39 (48%)
Query: 47 NTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVD 85
+ E + +R + Y Q + + Q + W DD+ KW D
Sbjct: 709 SAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKD 747
>GLS3_YEAST (Q04952) 1,3-beta-glucan synthase component FKS3 (EC
2.4.1.34) (1,3-beta-D-glucan-UDP glucosyltransferase)
Length = 1785
Score = 27.3 bits (59), Expect = 9.8
Identities = 10/45 (22%), Positives = 23/45 (50%)
Query: 45 FKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPK 89
F+N + K R M + + QI+ W++ ++++++PK
Sbjct: 105 FQNMRLHGKARQRNVKMAKKRGVSIKEQIKQWNEKEQEFINNHPK 149
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.329 0.139 0.467
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,161,505
Number of Sequences: 164201
Number of extensions: 586143
Number of successful extensions: 1744
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1729
Number of HSP's gapped (non-prelim): 18
length of query: 136
length of database: 59,974,054
effective HSP length: 98
effective length of query: 38
effective length of database: 43,882,356
effective search space: 1667529528
effective search space used: 1667529528
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC122727.10